##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087218
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29887842180685	34.0	31.0	34.0	30.0	34.0
2	32.49175326383485	34.0	31.0	34.0	30.0	34.0
3	32.580428212189275	34.0	31.0	34.0	31.0	34.0
4	36.09536817823105	37.0	35.0	37.0	35.0	37.0
5	35.92895261115986	37.0	35.0	37.0	35.0	37.0
6	35.890436876504985	37.0	35.0	37.0	35.0	37.0
7	35.96680702490209	37.0	35.0	37.0	35.0	37.0
8	36.06441118524528	37.0	35.0	37.0	35.0	37.0
9	37.710730506669314	39.0	38.0	39.0	35.0	39.0
10	37.27085552299539	39.0	37.0	39.0	34.0	39.0
11	37.20664209017879	39.0	37.0	39.0	34.0	39.0
12	37.13167460435717	39.0	37.0	39.0	33.0	39.0
13	37.12275459015579	39.0	37.0	39.0	33.0	39.0
14	38.36213344517843	40.0	38.0	41.0	34.0	41.0
15	38.35579708945216	40.0	38.0	41.0	34.0	41.0
16	38.34930161200422	40.0	38.0	41.0	34.0	41.0
17	38.316985186043645	40.0	38.0	41.0	34.0	41.0
18	38.25176643506638	40.0	37.0	41.0	34.0	41.0
19	38.235323550566676	40.0	37.0	41.0	34.0	41.0
20	38.1551758708925	40.0	37.0	41.0	33.0	41.0
21	38.12647601492985	40.0	37.0	41.0	33.0	41.0
22	38.057000527952994	40.0	37.0	41.0	33.0	41.0
23	37.97101869174352	40.0	37.0	41.0	33.0	41.0
24	37.88882818349218	40.0	37.0	41.0	33.0	41.0
25	37.83462930157521	40.0	37.0	41.0	33.0	41.0
26	37.7426652244536	40.0	37.0	41.0	33.0	41.0
27	37.66772901110909	40.0	36.0	41.0	33.0	41.0
28	37.59879159469398	40.0	36.0	41.0	33.0	41.0
29	37.48250488862399	40.0	36.0	41.0	32.0	41.0
30	37.424238745127475	40.0	36.0	41.0	32.0	41.0
31	37.37404366005714	40.0	36.0	41.0	32.0	41.0
32	37.321417599782194	39.0	36.0	41.0	32.0	41.0
33	37.228616524008984	39.0	36.0	41.0	32.0	41.0
34	37.11913802015787	39.0	35.0	41.0	31.0	41.0
35	37.06783828082317	39.0	35.0	41.0	31.0	41.0
36	36.94583606967508	39.0	35.0	41.0	31.0	41.0
37	36.873085250612114	39.0	35.0	41.0	31.0	41.0
38	36.807481112343616	39.0	35.0	41.0	31.0	41.0
39	36.730952762003575	39.0	35.0	41.0	31.0	41.0
40	36.63536935554783	39.0	35.0	41.0	30.0	41.0
41	36.56265716719186	39.0	35.0	41.0	30.0	41.0
42	36.45994455573767	39.0	35.0	41.0	30.0	41.0
43	36.39099794153518	39.0	35.0	40.0	30.0	41.0
44	36.28098044734359	39.0	35.0	40.0	30.0	41.0
45	36.158539501737465	38.0	35.0	40.0	30.0	41.0
46	36.044323217606774	38.0	35.0	40.0	29.0	41.0
47	35.88305289279611	38.0	35.0	40.0	29.0	41.0
48	35.76963221727381	38.0	35.0	40.0	29.0	41.0
49	35.64969490939259	38.0	34.0	40.0	28.0	41.0
50	35.532184897601034	38.0	34.0	40.0	28.0	41.0
51	34.43223990036957	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	7.0
12	13.0
13	17.0
14	38.0
15	65.0
16	109.0
17	203.0
18	361.0
19	629.0
20	992.0
21	1614.0
22	2196.0
23	3158.0
24	4290.0
25	5546.0
26	7062.0
27	8783.0
28	10980.0
29	13971.0
30	18051.0
31	23282.0
32	30044.0
33	40888.0
34	64537.0
35	78701.0
36	92075.0
37	134727.0
38	221343.0
39	323404.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.644693152615208	32.335189446826675	19.102332742835383	26.917784657722738
2	24.68851693036723	29.201687242117035	19.261178530892607	26.84861729662312
3	21.71422842521003	30.5707778936699	20.308898491378912	27.40609518974115
4	22.28062817208692	30.76393142865552	19.090927486483853	27.864512912773705
5	20.44107069603336	33.06742530016979	16.590325031410444	29.901178972386404
6	20.938762971179653	38.04370420651608	18.849209634130414	22.16832318817385
7	85.19266605225447	5.918132334085712	4.712210430658801	4.176991183001017
8	86.38837841168929	5.498529273797895	4.4221122166851545	3.6909800978276666
9	78.19719688231798	9.281395267554437	7.285475406036324	5.235932444091249
10	34.82236313232489	33.178534571723425	15.628512405055838	16.370589890895847
11	26.25673967870289	31.43950891173619	24.9279353358756	17.375816073685314
12	26.843466535690176	25.88294159956881	28.71135319687496	18.56223866786606
13	24.781046671412724	26.100193337490733	27.959893967907078	21.158866023189464
14	21.495688997054867	28.392557886274876	28.908645736181704	21.20310738048855
15	21.316240165265842	29.29688434150281	29.431447970876125	19.955427522355222
16	22.62388959711852	28.439466601914244	28.837914751227444	20.098729049739795
17	22.311072848315607	27.778145689272986	29.25834561237949	20.652435850031917
18	22.561620576554102	28.10954196858404	28.42208278376554	20.90675467109632
19	22.938821837018885	28.502747379090486	28.13805510946287	20.42037567442776
20	23.533918680522213	28.417943779444414	27.763889118833575	20.284248421199795
21	24.66129147972164	28.607418199477934	27.024203057712437	19.707087263087992
22	23.186702206917104	27.974610427715508	26.787176076922936	22.051511288444452
23	22.505973962903482	29.273521961556924	27.27475078595093	20.945753289588655
24	21.50911776663006	28.780152646479362	28.368735616959984	21.34199396993059
25	21.923754021732535	29.710876751488662	26.566613135544117	21.79875609123469
26	23.06565932499278	28.921706594261686	26.946205820727766	21.06642826001777
27	21.953738808592206	28.411045438909216	27.508558541157342	22.12665721134124
28	21.99963576761974	29.641249501020035	27.800588290480842	20.558526440879383
29	22.233719456447556	30.321885767159852	26.276882833065677	21.167511943326915
30	22.479668290995917	28.614408517886936	27.542038487221514	21.36388470389563
31	22.592249208530397	28.787418898509777	26.357179516895417	22.26315237606441
32	22.56290826678734	29.13941822155262	27.401588273924826	20.89608523773521
33	22.336366763611345	28.624526084005232	27.299308878256245	21.739798274127175
34	21.38890268556996	29.26395626268145	28.167120117584517	21.18002093416408
35	22.439290004396543	28.86458833463022	28.67299842349924	20.023123237473996
36	22.760200806094087	31.69897849373355	26.81228603647107	18.7285346637013
37	22.03872636398588	30.947335309018065	27.168332385961236	19.845605941034826
38	22.25091931884866	29.458305510026506	28.44038638065227	19.850388790472564
39	21.738326628146336	28.787694832131184	28.034120112065843	21.439858427656645
40	22.466791388663545	29.394104954112237	28.277493566147726	19.86161009107649
41	20.681868769648773	28.910025404288742	30.16506349232629	20.2430423337362
42	21.50442689506612	29.027573127008566	28.29266991532517	21.175330062600143
43	21.38034874330631	28.762124983214036	28.525741847541152	21.331784425938498
44	22.051235354823042	28.732508107849576	27.48252880287118	21.7337277344562
45	22.273729831551723	28.64476121624182	27.89808483671168	21.183424115494777
46	20.709002242420564	29.72191409634498	28.485363560941778	21.083720100292673
47	21.33086464720047	29.874321433236023	28.3485924625972	20.446221456966313
48	21.182688292504356	29.755118108787748	28.13014501231584	20.932048586392057
49	20.227590050937348	29.653574536109595	28.621858725664957	21.496976687288104
50	20.56294137882191	29.000531632110583	28.93890645666278	21.497620532404724
51	20.579589373980195	28.31575636164964	28.812896769553113	22.29175749481705
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5621.0
1	4384.0
2	3147.0
3	2238.0
4	1329.0
5	1305.5
6	1282.0
7	1415.0
8	1548.0
9	1777.0
10	2006.0
11	2376.0
12	2746.0
13	3138.5
14	3531.0
15	3883.0
16	4235.0
17	3953.5
18	3672.0
19	3972.0
20	4272.0
21	5162.0
22	6052.0
23	6996.0
24	7940.0
25	9542.0
26	13514.0
27	15884.0
28	18628.0
29	21372.0
30	27371.0
31	33370.0
32	33534.5
33	33699.0
34	39359.0
35	45019.0
36	47047.5
37	49076.0
38	51278.5
39	53481.0
40	56660.5
41	59840.0
42	62444.0
43	65048.0
44	68409.0
45	71770.0
46	75210.0
47	78650.0
48	77750.5
49	76851.0
50	71840.0
51	66829.0
52	62096.0
53	57363.0
54	54358.0
55	51353.0
56	47878.0
57	44403.0
58	43216.0
59	42029.0
60	40647.0
61	39265.0
62	36719.5
63	34174.0
64	30279.0
65	26384.0
66	21961.5
67	17539.0
68	15624.0
69	13709.0
70	12153.0
71	10597.0
72	9216.5
73	7836.0
74	6338.5
75	4298.5
76	3756.0
77	3026.0
78	2296.0
79	1718.0
80	1140.0
81	849.5
82	559.0
83	425.5
84	292.0
85	208.5
86	125.0
87	91.0
88	57.0
89	47.5
90	38.0
91	31.5
92	25.0
93	18.5
94	12.0
95	10.5
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1087218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.02937538929817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24595609077355	17.600545746532482
2	8.171554124182618	3.927146831279036
3	3.3585492874292675	2.4211152477329327
4	1.9750345563998	1.8983538705026715
5	1.343950935087504	1.614715076200797
6	1.0425786547014226	1.5031508320013978
7	0.8419325778754088	1.41617797763734
8	0.714650740074737	1.3738088724396487
9	0.6387961097517958	1.3814884366604294
>10	7.398821486385604	38.86341392742681
>50	0.9353563460784204	15.545257167693007
>100	0.32781718584213115	11.107003982800643
>500	0.003847619552137666	0.6529235274781932
>1k	0.0011542858656412999	0.6948985036145711
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4303	0.3957807909729235	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2062	0.1896583757811221	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1151	0.1058665327468824	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10062379394012977	0.0
2	0.0	0.0	0.0	0.15479876160990713	0.0
3	9.197787380267803E-5	0.0	0.0	0.20998548589151395	0.0
4	9.197787380267803E-5	0.0	0.0	0.43560721032948313	0.0
5	9.197787380267803E-5	0.0	0.0	0.49410513806798634	0.0
6	9.197787380267803E-5	0.0	0.0	0.8897019732933046	0.0
7	9.197787380267803E-5	0.0	0.0	1.1718900901199207	0.0
8	9.197787380267803E-5	0.0	0.0	1.5175429398703848	0.0
9	9.197787380267803E-5	0.0	0.0	1.8729454442439326	0.0
10	9.197787380267803E-5	0.0	0.0	2.5054772823849496	0.0
11	9.197787380267803E-5	0.0	0.0	2.927839678886847	0.0
12	9.197787380267803E-5	0.0	0.0	3.1289952888933037	0.0
13	1.8395574760535606E-4	0.0	0.0	3.234034020775962	0.0
14	1.8395574760535606E-4	0.0	0.0	3.333830013851868	0.0
15	1.8395574760535606E-4	0.0	0.0	3.418541635624134	0.0
16	1.8395574760535606E-4	0.0	0.0	3.5710409503889746	0.0
17	1.8395574760535606E-4	0.0	0.0	3.7437753973904035	0.0
18	1.8395574760535606E-4	0.0	0.0	3.9636025157788044	0.0
19	2.759336214080341E-4	0.0	0.0	4.059167526659786	0.0
20	2.759336214080341E-4	0.0	0.0	4.1852691916432585	0.0
21	2.759336214080341E-4	0.0	0.0	4.315049971578837	0.0
22	2.759336214080341E-4	0.0	0.0	4.460742923682279	0.0
23	2.759336214080341E-4	0.0	0.0	4.613978061437541	0.0
24	3.679114952107121E-4	0.0	0.0	4.7464261997133965	0.0
25	3.679114952107121E-4	0.0	0.0	4.857903382762243	0.0
26	3.679114952107121E-4	0.0	0.0	4.964873649994757	0.0
27	3.679114952107121E-4	0.0	0.0	5.083249173578804	0.0
28	3.679114952107121E-4	0.0	0.0	5.209258860688473	0.0
29	3.679114952107121E-4	0.0	0.0	5.337384038895603	0.0
30	3.679114952107121E-4	0.0	0.0	5.504507835595069	0.0
31	3.679114952107121E-4	0.0	0.0	5.633184881045016	0.0
32	3.679114952107121E-4	0.0	0.0	5.778693877400852	0.0
33	3.679114952107121E-4	0.0	0.0	5.919512002192753	0.0
34	3.679114952107121E-4	0.0	0.0	6.058674525256205	0.0
35	3.679114952107121E-4	0.0	0.0	6.208046592311754	0.0
36	3.679114952107121E-4	0.0	0.0	6.357602615114908	0.0
37	3.679114952107121E-4	0.0	0.0	6.504215345956377	0.0
38	3.679114952107121E-4	0.0	0.0	6.666740248965708	0.0
39	3.679114952107121E-4	0.0	0.0	6.8364394261316495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	30	2.1653923E-6	45.000004	1
CGACTTA	20	7.0331176E-4	45.0	27
TAGGACG	20	7.0331176E-4	45.0	1
GTAACGC	20	7.0331176E-4	45.0	22
ATGCCCG	65	0.0	44.999996	1
ATACGCG	85	0.0	42.35294	1
ATATCCG	65	0.0	41.53846	1
AGTACGG	195	0.0	41.53846	2
TGCGACG	45	1.9281288E-8	40.0	1
ACGGGTA	335	0.0	39.626865	5
CTCGTAG	40	3.4579898E-7	39.375	1
TGACGGG	400	0.0	39.375	3
TAGTACG	40	3.4579898E-7	39.375	1
CGAGGGA	610	0.0	39.098362	4
CGGTAGT	105	0.0	38.57143	12
TCGAACG	35	6.2479776E-6	38.571426	1
AGCACGT	35	6.2479776E-6	38.571426	18
ATAGGGC	375	0.0	38.399998	4
TAGGGCG	160	0.0	37.96875	5
TACGGGA	580	0.0	37.62931	4
>>END_MODULE