FastQCFastQC Report
Sat 18 Jun 2016
SRR3554103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058398
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41910.39597580494294204No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA19980.18877586692340687No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11190.10572582336701315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG351.2118107E-745.0000041
CGACTTA253.890595E-545.00000427
CGCAATG950.045.01
CGATCTC207.033034E-445.036
CGATAGT207.033034E-445.016
TACGTCC207.033034E-445.041
CGTATAG302.1653377E-644.9999961
GCGATTC302.1653377E-644.9999969
TTCGATC302.1653377E-644.99999613
CGCGACG850.042.3529431
TACGGGT2500.041.44
CGTAACG603.6379788E-1241.2499961
TTAACGG1500.040.52
AGTACGG1850.040.1351362
CTTGCGG2250.040.02
TACGGGA7200.039.3754
ACGTACG403.4578807E-739.3751
TCGAACG403.4578807E-739.3751
TATGCGG1200.039.3749962
CCGTATA356.2478193E-638.5714333
Produced by FastQC (version 0.11.5)