##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1058398
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.373173418695046	34.0	31.0	34.0	30.0	34.0
2	32.55184722571282	34.0	31.0	34.0	31.0	34.0
3	32.63059076075351	34.0	31.0	34.0	31.0	34.0
4	36.1253082488818	37.0	35.0	37.0	35.0	37.0
5	35.97499617346216	37.0	35.0	37.0	35.0	37.0
6	35.94769736904265	37.0	35.0	37.0	35.0	37.0
7	35.99678948750848	37.0	35.0	37.0	35.0	37.0
8	36.09724886101448	37.0	35.0	37.0	35.0	37.0
9	37.73948080022827	39.0	38.0	39.0	35.0	39.0
10	37.32359755025992	39.0	37.0	39.0	34.0	39.0
11	37.26756002940293	39.0	37.0	39.0	34.0	39.0
12	37.209261544333984	39.0	37.0	39.0	34.0	39.0
13	37.20187490906068	39.0	37.0	39.0	34.0	39.0
14	38.44999423657263	40.0	38.0	41.0	34.0	41.0
15	38.44370643179598	40.0	38.0	41.0	34.0	41.0
16	38.44206810670466	40.0	38.0	41.0	34.0	41.0
17	38.40950001795166	40.0	38.0	41.0	34.0	41.0
18	38.343622153481014	40.0	38.0	41.0	34.0	41.0
19	38.3384426274426	40.0	38.0	41.0	34.0	41.0
20	38.25840657295271	40.0	37.0	41.0	34.0	41.0
21	38.22798795916092	40.0	37.0	41.0	34.0	41.0
22	38.16589978439113	40.0	37.0	41.0	34.0	41.0
23	38.07266642605145	40.0	37.0	41.0	33.0	41.0
24	38.02375193452747	40.0	37.0	41.0	33.0	41.0
25	37.96435178448939	40.0	37.0	41.0	33.0	41.0
26	37.90492045525407	40.0	37.0	41.0	33.0	41.0
27	37.83132432223039	40.0	37.0	41.0	33.0	41.0
28	37.7226185234666	40.0	36.0	41.0	33.0	41.0
29	37.64424441467199	40.0	36.0	41.0	33.0	41.0
30	37.58717609065777	40.0	36.0	41.0	33.0	41.0
31	37.50619332236077	40.0	36.0	41.0	33.0	41.0
32	37.46491584451218	40.0	36.0	41.0	33.0	41.0
33	37.38268779797392	40.0	36.0	41.0	32.0	41.0
34	37.275641110432936	40.0	36.0	41.0	32.0	41.0
35	37.19075432871188	39.0	35.0	41.0	32.0	41.0
36	37.09898922711494	39.0	35.0	41.0	31.0	41.0
37	37.02773814765334	39.0	35.0	41.0	31.0	41.0
38	36.96112143069053	39.0	35.0	41.0	31.0	41.0
39	36.88366285650577	39.0	35.0	41.0	31.0	41.0
40	36.771330822620605	39.0	35.0	41.0	31.0	41.0
41	36.706687843325476	39.0	35.0	41.0	31.0	41.0
42	36.6204905904962	39.0	35.0	41.0	31.0	41.0
43	36.53790256595345	39.0	35.0	41.0	30.0	41.0
44	36.441330199036656	39.0	35.0	41.0	30.0	41.0
45	36.31595108834295	39.0	35.0	40.0	30.0	41.0
46	36.16830625152353	38.0	35.0	40.0	30.0	41.0
47	36.05834194698025	38.0	35.0	40.0	30.0	41.0
48	35.94797136804869	38.0	35.0	40.0	29.0	41.0
49	35.84084814975085	38.0	35.0	40.0	29.0	41.0
50	35.75357474220473	38.0	34.0	40.0	29.0	41.0
51	34.70162264101028	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	6.0
11	8.0
12	16.0
13	18.0
14	32.0
15	56.0
16	106.0
17	195.0
18	310.0
19	511.0
20	873.0
21	1347.0
22	2089.0
23	2808.0
24	3858.0
25	5011.0
26	6397.0
27	8010.0
28	10084.0
29	12999.0
30	16643.0
31	21375.0
32	27619.0
33	38034.0
34	60444.0
35	75046.0
36	86913.0
37	128462.0
38	213523.0
39	335414.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.696186122800686	32.24165200614514	19.03612818618327	27.02603368487091
2	24.79492591633771	29.150848735541828	19.14903467315698	26.90519067496348
3	21.71867293777955	30.49523903106393	20.23596038541267	27.55012764574385
4	22.321470751078518	30.634506112067484	19.134295416280075	27.909727720573922
5	20.57732535397837	32.995243755184724	16.599048751036943	29.828382139799963
6	21.002968637506875	37.855041298263984	18.860957787146233	22.281032277082915
7	85.18903096944628	5.8098182347283345	4.734513859625586	4.266636936199804
8	86.39179212356788	5.409307273823269	4.423761193804221	3.7751394088046273
9	78.16681437417682	9.270803610740005	7.254643338328304	5.307738676754869
10	34.906245098724675	33.064499366022986	15.646571516575051	16.382684018677285
11	26.326485877713303	31.377893760192286	24.824120982843883	17.471499379250528
12	26.8975375992774	25.812123605675747	28.699506234894624	18.59083256015223
13	24.856717416321647	26.064958550564153	27.97189715022137	21.106426882892826
14	21.60945126502507	28.340850984223326	28.97189903986969	21.07779871088192
15	21.40518028189774	29.149903911383053	29.46481380350303	19.98010200321618
16	22.66000124716789	28.43004238481176	28.825640260091195	20.084316107929155
17	22.380144331338496	27.73654145227032	29.177776224066935	20.70553799232425
18	22.62513723570906	28.004115654035626	28.51564345359685	20.855103656658457
19	22.963384284550802	28.388470121825627	28.19695426484177	20.4511913287818
20	23.559851775985972	28.325450350435283	27.79341986662862	20.321278006950127
21	24.71395448593062	28.52953236873085	27.04360741422414	19.712905731114382
22	23.281506578810617	27.86503753786383	26.7694194433474	22.084036439978156
23	22.500042517087145	29.3018316361142	27.326393284945738	20.871732561852912
24	21.604727144231187	28.739188849563206	28.35039370822696	21.305690297978643
25	21.89507160822299	29.56175276219343	26.688920425019703	21.85425520456388
26	23.084416259289984	28.790020389305347	27.046441886700467	21.079121464704205
27	21.92492805164031	28.39064321739081	27.59897505475256	22.085453676216318
28	22.123908019478495	29.558918289717102	27.7488241663344	20.56834952447
29	22.26780473886005	30.247600619048786	26.298424600197656	21.186170041893504
30	22.54718924261006	28.600583145470797	27.5583476159252	21.293879995993944
31	22.72292653614236	28.658311901572	26.30730594729015	22.31145561499549
32	22.567219514776106	29.106158552831733	27.369477266585918	20.957144665806247
33	22.269221975098212	28.547861957411108	27.33782565726693	21.84509041022375
34	21.434186383572154	29.195255471004288	28.173522625704127	21.197035519719424
35	22.516482457449843	28.80041345505188	28.64149403154579	20.041610055952486
36	22.68853493676292	31.64537347954172	26.853036381399058	18.8130552022963
37	22.108034973611062	30.804763425478882	27.107477527357382	19.97972407355267
38	22.326005907040642	29.437791832562045	28.41690932900478	19.81929293139254
39	21.797660237453208	28.73172473870888	27.98474675878072	21.48586826505719
40	22.517616246440376	29.26951864988407	28.30825455074556	19.904610552929995
41	20.708183499968822	28.875621458090432	30.199320104535342	20.2168749374054
42	21.667274503542146	28.98333141219088	28.249958900149092	21.099435184117883
43	21.40234580942141	28.731913703540634	28.52169032821302	21.34405015882494
44	22.15329205081642	28.732669562867653	27.444874234456222	21.669164151859697
45	22.37882157751621	28.582442521622305	27.910861509564455	21.127874391297034
46	20.680311187284932	29.640929026698842	28.47558290926476	21.203176876751467
47	21.38392173832528	29.768952700212964	28.329229647070385	20.517895914391374
48	21.18560314739824	29.629307689545897	28.177679852002747	21.00740931105312
49	20.38051848170537	29.61362360851022	28.544649555271267	21.461208354513143
50	20.637699617724145	28.917288203492447	28.93675158116323	21.508260597620176
51	20.624283114669527	28.197332194505282	28.93079918896294	22.24758550186225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5405.0
1	4165.0
2	2925.0
3	2072.5
4	1220.0
5	1263.0
6	1306.0
7	1439.0
8	1572.0
9	1783.5
10	1995.0
11	2311.0
12	2627.0
13	2955.5
14	3284.0
15	3708.5
16	4133.0
17	3790.0
18	3447.0
19	3798.5
20	4150.0
21	4977.5
22	5805.0
23	6744.5
24	7684.0
25	9130.5
26	13014.5
27	15452.0
28	17859.5
29	20267.0
30	26343.5
31	32420.0
32	32570.0
33	32720.0
34	38156.5
35	43593.0
36	45581.0
37	47569.0
38	49938.0
39	52307.0
40	55153.0
41	57999.0
42	60669.5
43	63340.0
44	66474.5
45	69609.0
46	73001.5
47	76394.0
48	75837.0
49	75280.0
50	70264.5
51	65249.0
52	60530.0
53	55811.0
54	52786.0
55	49761.0
56	46794.0
57	43827.0
58	42184.5
59	40542.0
60	39619.0
61	38696.0
62	36076.5
63	33457.0
64	29529.5
65	25602.0
66	21581.5
67	17561.0
68	15615.0
69	13669.0
70	12173.0
71	10677.0
72	9172.5
73	7668.0
74	6220.0
75	4199.5
76	3627.0
77	2959.5
78	2292.0
79	1684.5
80	1077.0
81	803.5
82	530.0
83	389.0
84	248.0
85	181.0
86	114.0
87	80.5
88	47.0
89	46.0
90	45.0
91	37.0
92	29.0
93	20.0
94	11.0
95	7.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1058398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.443465246802777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28141124744691	16.945267525693815
2	8.319086791040668	3.9005644414179583
3	3.3965015915967163	2.3887730106992384
4	2.037494382549763	1.9106371499144503
5	1.4726221487133362	1.7261683082516566
6	1.1136976071803095	1.56653586896274
7	0.8981170187744028	1.473848258203988
8	0.7880710545358491	1.4780093103217917
9	0.6329020133565907	1.3353674719281068
>10	7.763264757316028	39.71427256362371
>50	0.9707964483131712	15.734720222169834
>100	0.32075666494299243	10.477629661102874
>500	0.00406021094864547	0.6525912002300344
>1k	0.0012180632845936409	0.695615007479739
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4191	0.39597580494294204	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1998	0.18877586692340687	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1119	0.10572582336701315	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.448241587758102E-5	0.0	0.0	0.10326928055419606	0.0
2	9.448241587758102E-5	0.0	0.0	0.15825804659494822	0.0
3	9.448241587758102E-5	0.0	0.0	0.21825438067721217	0.0
4	9.448241587758102E-5	0.0	0.0	0.45427145553940956	0.0
5	9.448241587758102E-5	0.0	0.0	0.5189919104155526	0.0
6	9.448241587758102E-5	0.0	0.0	0.910810489059881	0.0
7	9.448241587758102E-5	0.0	0.0	1.2061625210931994	0.0
8	9.448241587758102E-5	0.0	0.0	1.5535743642750648	0.0
9	9.448241587758102E-5	0.0	0.0	1.9182764895625275	0.0
10	9.448241587758102E-5	0.0	0.0	2.5394983739576227	0.0
11	9.448241587758102E-5	0.0	0.0	2.9619292553462877	0.0
12	9.448241587758102E-5	0.0	0.0	3.1662002384736176	0.0
13	9.448241587758102E-5	0.0	0.0	3.275988805723367	0.0
14	9.448241587758102E-5	0.0	0.0	3.3795415335251957	0.0
15	9.448241587758102E-5	0.0	0.0	3.462213647418079	0.0
16	9.448241587758102E-5	0.0	0.0	3.613574477653964	0.0
17	9.448241587758102E-5	0.0	0.0	3.7831704141542217	0.0
18	9.448241587758102E-5	0.0	0.0	4.006432362872946	0.0
19	9.448241587758102E-5	0.0	0.0	4.109134748931877	0.0
20	9.448241587758102E-5	0.0	0.0	4.23593015103959	0.0
21	9.448241587758102E-5	0.0	0.0	4.362631070731426	0.0
22	9.448241587758102E-5	0.0	0.0	4.511818805402126	0.0
23	9.448241587758102E-5	0.0	0.0	4.671588570651116	0.0
24	9.448241587758102E-5	0.0	0.0	4.800273621076381	0.0
25	9.448241587758102E-5	0.0	0.0	4.912140801475437	0.0
26	9.448241587758102E-5	0.0	0.0	5.025330735696779	0.0
27	9.448241587758102E-5	0.0	0.0	5.146173745604206	0.0
28	9.448241587758102E-5	0.0	0.0	5.269662263156204	0.0
29	9.448241587758102E-5	0.0	0.0	5.403071434375349	0.0
30	9.448241587758102E-5	0.0	0.0	5.570683240142177	0.0
31	9.448241587758102E-5	0.0	0.0	5.704659305856587	0.0
32	9.448241587758102E-5	0.0	0.0	5.847233271415857	0.0
33	9.448241587758102E-5	0.0	0.0	5.99009068422276	0.0
34	9.448241587758102E-5	0.0	0.0	6.129452247642191	0.0
35	9.448241587758102E-5	0.0	0.0	6.285253751424323	0.0
36	9.448241587758102E-5	0.0	0.0	6.429622882885266	0.0
37	1.8896483175516205E-4	0.0	0.0	6.574842356089108	0.0
38	1.8896483175516205E-4	0.0	0.0	6.737352111398548	0.0
39	1.8896483175516205E-4	0.0	0.0	6.9059087413241524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAG	35	1.2118107E-7	45.000004	1
CGACTTA	25	3.890595E-5	45.000004	27
CGCAATG	95	0.0	45.0	1
CGATCTC	20	7.033034E-4	45.0	36
CGATAGT	20	7.033034E-4	45.0	16
TACGTCC	20	7.033034E-4	45.0	41
CGTATAG	30	2.1653377E-6	44.999996	1
GCGATTC	30	2.1653377E-6	44.999996	9
TTCGATC	30	2.1653377E-6	44.999996	13
CGCGACG	85	0.0	42.352943	1
TACGGGT	250	0.0	41.4	4
CGTAACG	60	3.6379788E-12	41.249996	1
TTAACGG	150	0.0	40.5	2
AGTACGG	185	0.0	40.135136	2
CTTGCGG	225	0.0	40.0	2
TACGGGA	720	0.0	39.375	4
ACGTACG	40	3.4578807E-7	39.375	1
TCGAACG	40	3.4578807E-7	39.375	1
TATGCGG	120	0.0	39.374996	2
CCGTATA	35	6.2478193E-6	38.57143	33
>>END_MODULE