FastQCFastQC Report
Sat 18 Jun 2016
SRR3554099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778752
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27520.3533859303090072No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT8100.10401257396449705No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7830.10054548816568047No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA207.0318824E-445.00000440
CGTTACG253.8896425E-545.01
CTACGAA351.2112469E-745.010
CGTAACG253.8896425E-545.01
CAATGCG253.8896425E-545.01
ATACGCG253.8896425E-545.01
AATGCGG603.6379788E-1241.2499962
TAACACG1100.040.909091
ACTCACG501.0804797E-940.51
ACGGGTA1850.040.1351365
CGAACAG403.4562981E-739.3750041
TATACCG403.4562981E-739.3750041
TAATCGC403.4562981E-739.37500443
ATGGTCG750.039.044
AACGGGA8550.038.9473654
TAACGCC700.038.57142612
CGTTCGA356.2456966E-638.57142614
ATGTACG700.038.5714261
TACGAAG700.038.5714261
CAAACGG2300.038.1521762
Produced by FastQC (version 0.11.5)