##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554098_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1053773 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.978810426913576 33.0 31.0 34.0 30.0 34.0 2 32.18633045257375 34.0 31.0 34.0 30.0 34.0 3 32.324856491863045 34.0 31.0 34.0 30.0 34.0 4 35.92566710287699 37.0 35.0 37.0 35.0 37.0 5 35.725791038487415 37.0 35.0 37.0 33.0 37.0 6 35.72920828299833 37.0 35.0 37.0 33.0 37.0 7 35.881870194055075 37.0 35.0 37.0 35.0 37.0 8 36.05064942829243 37.0 35.0 37.0 35.0 37.0 9 37.691350034589995 39.0 38.0 39.0 35.0 39.0 10 37.180038774954376 39.0 37.0 39.0 34.0 39.0 11 37.13160898979192 39.0 37.0 39.0 34.0 39.0 12 37.15454182257469 39.0 37.0 39.0 34.0 39.0 13 37.15621960327319 39.0 37.0 39.0 33.0 39.0 14 38.41067952965202 40.0 38.0 41.0 34.0 41.0 15 38.386197027253495 40.0 38.0 41.0 34.0 41.0 16 38.30456369635586 40.0 38.0 41.0 33.0 41.0 17 38.3197633645956 40.0 38.0 41.0 34.0 41.0 18 38.2810045427241 40.0 38.0 41.0 33.0 41.0 19 38.294980038395366 40.0 38.0 41.0 34.0 41.0 20 38.23249883988297 40.0 38.0 41.0 34.0 41.0 21 38.203634938454485 40.0 37.0 41.0 34.0 41.0 22 38.205146649230905 40.0 37.0 41.0 34.0 41.0 23 38.09769751170318 40.0 37.0 41.0 33.0 41.0 24 38.02120855250609 40.0 37.0 41.0 33.0 41.0 25 38.03216252456649 40.0 37.0 41.0 33.0 41.0 26 37.98375076985271 40.0 37.0 41.0 33.0 41.0 27 37.935230832446834 40.0 37.0 41.0 33.0 41.0 28 37.917761225615 40.0 37.0 41.0 33.0 41.0 29 37.837036059948396 40.0 37.0 41.0 33.0 41.0 30 37.77839060215056 40.0 37.0 41.0 33.0 41.0 31 37.70310493816031 40.0 37.0 41.0 33.0 41.0 32 37.672773927591614 40.0 37.0 41.0 33.0 41.0 33 37.61080327546824 40.0 37.0 41.0 33.0 41.0 34 37.55984638057722 40.0 37.0 41.0 32.0 41.0 35 37.53015212953834 40.0 37.0 41.0 32.0 41.0 36 37.46613929185887 40.0 36.0 41.0 32.0 41.0 37 37.42839017511361 40.0 36.0 41.0 32.0 41.0 38 37.47085852455889 40.0 36.0 41.0 32.0 41.0 39 37.39791586992644 40.0 36.0 41.0 32.0 41.0 40 37.315815645305015 39.0 36.0 41.0 32.0 41.0 41 37.236496854635675 39.0 36.0 41.0 32.0 41.0 42 37.16979842907343 39.0 36.0 41.0 31.0 41.0 43 37.12779507540998 39.0 36.0 41.0 31.0 41.0 44 37.03272241744664 39.0 36.0 41.0 31.0 41.0 45 36.90704259835847 39.0 35.0 41.0 31.0 41.0 46 36.79983449946051 39.0 35.0 40.0 31.0 41.0 47 36.732875106877856 39.0 35.0 40.0 31.0 41.0 48 36.67536366940508 39.0 35.0 40.0 31.0 41.0 49 36.52153357506788 39.0 35.0 40.0 30.0 41.0 50 36.40289132479196 39.0 35.0 40.0 30.0 41.0 51 35.29036993735843 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 0.0 10 9.0 11 13.0 12 6.0 13 9.0 14 18.0 15 26.0 16 32.0 17 79.0 18 152.0 19 321.0 20 569.0 21 909.0 22 1384.0 23 1970.0 24 2712.0 25 3599.0 26 4872.0 27 6429.0 28 8730.0 29 11682.0 30 15520.0 31 20855.0 32 27186.0 33 36931.0 34 54880.0 35 69578.0 36 90216.0 37 135772.0 38 229361.0 39 329840.0 40 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.520971784245756 32.929293121004235 21.336189103345788 27.21354599140422 2 20.2584427575958 30.77199738463597 21.884979022996415 27.084580834771817 3 19.967772945406647 31.614304029425693 22.10001584781542 26.31790717735224 4 20.017309230735652 31.693638003630763 19.580023401624448 28.709029364009137 5 18.566142803051513 34.888348818958164 17.599615856545956 28.94589252144437 6 20.663938058765975 36.108630606401945 18.217680657978523 25.009750676853553 7 88.2768869576275 3.4315739727626347 4.218270917930143 4.073268151679725 8 89.92335161367771 3.008332914204482 3.2503205149496144 3.817994957168195 9 81.85975537425992 8.115030466713419 6.462587293468328 3.562626865558332 10 30.836906999894666 44.78402843876243 12.356171585341436 12.022892976001472 11 19.859780047505488 25.87559180202947 36.10540410505868 18.159224045406365 12 25.424166305266883 22.966521252679655 31.829435751343034 19.77987669071043 13 23.81509110595925 24.27477265027667 29.742553661936675 22.167582581827393 14 18.913561080042857 26.155917830500496 30.61722021725742 24.313300872199232 15 18.23096625174492 31.43475871938264 29.7036458516208 20.63062917725165 16 20.9693169211965 29.81068977853864 29.09307792095641 20.126915379308446 17 20.086204524124266 30.43036783064284 29.57458579789006 19.908841847342835 18 19.71354361897676 29.92039082420977 27.873555310299274 22.49251024651419 19 19.809864173783158 32.14183699905008 28.173145449731585 19.87515337743518 20 21.689396103335348 30.629177251647178 28.064772963437097 19.616653681580377 21 21.713689760508192 30.688867526497642 27.62435553008096 19.973087182913208 22 21.618887559275098 27.70805477080927 28.376225240160828 22.296832429754794 23 20.917598002605875 28.77593181833279 28.06638621410873 22.240083964952603 24 18.877500182676915 29.10911553057442 30.04897639244885 21.964407894299818 25 19.94272011144715 31.432576086121017 25.87948258306106 22.745221219370777 26 21.043431554993344 32.187672297544154 25.522859287531563 21.246036859930932 27 19.317158439246406 31.45184019708229 26.5186145403232 22.7123868233481 28 19.681753091035738 30.874201559538918 28.303723857035624 21.140321492389727 29 21.63872105282637 28.033456920987724 26.33071828562698 23.997103740558927 30 22.008440147925597 30.220265654937066 25.291025676307893 22.480268520829437 31 20.523775044530463 31.866160928397292 23.8955638453443 23.714500181727942 32 22.700619583155003 30.256421449401344 24.475859601640963 22.567099365802694 33 20.723913024911436 30.30244654209208 25.271571771149954 23.702068661846525 34 19.58628661011432 29.732779260808538 26.300446111259255 24.38048801781788 35 20.327243153886084 29.162922185328338 25.762474460818414 24.747360199967165 36 19.666284864007714 32.02122278707084 25.34046706453857 22.97202528438288 37 20.067414898654643 29.038227398120846 27.520348310309718 23.374009392914793 38 19.983620760827996 27.171791268138396 28.860295338749424 23.98429263228418 39 19.125750991911918 28.51809640216631 27.635553387684062 24.720599218237705 40 20.979660704914625 28.914101993503344 27.466541655555798 22.63969564602623 41 18.910334578699587 28.215944041079055 30.643886301888546 22.229835078332812 42 20.035624370713617 28.88193187716899 27.839961737489954 23.242482014627438 43 19.27236700883397 29.586163243886492 27.99616236134348 23.14530738593606 44 21.673073802422344 28.044844572787497 26.03359547075129 24.24848615403887 45 21.162147825005956 28.38362721383068 26.39999316740892 24.054231793754443 46 19.0571403898183 29.557883908583726 27.06303919345058 24.32193650814739 47 20.027463220257115 28.409249430380168 27.503741318101717 24.059546031261004 48 20.27894053083539 26.708693428281045 29.679067503152957 23.333298537730613 49 20.15965487823279 27.78387755237608 27.717544480642413 24.338923088748714 50 19.336422550207683 25.99231523297712 28.28882501259759 26.382437204217606 51 18.81259056741822 25.445043666899796 29.618238463122516 26.12412730255947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 838.0 1 731.0 2 624.0 3 495.5 4 367.0 5 442.5 6 518.0 7 610.0 8 702.0 9 835.0 10 968.0 11 1215.5 12 1463.0 13 1618.5 14 1774.0 15 1872.0 16 1970.0 17 2244.0 18 2518.0 19 2757.0 20 2996.0 21 3273.0 22 3550.0 23 4397.0 24 5244.0 25 6236.0 26 10038.0 27 12848.0 28 15712.5 29 18577.0 30 21461.0 31 24345.0 32 29254.0 33 34163.0 34 38788.0 35 43413.0 36 46093.5 37 48774.0 38 52075.0 39 55376.0 40 57553.5 41 59731.0 42 62140.0 43 64549.0 44 70917.5 45 77286.0 46 84736.5 47 92187.0 48 98116.5 49 104046.0 50 99360.5 51 94675.0 52 85262.5 53 75850.0 54 65933.0 55 56016.0 56 48153.0 57 40290.0 58 37720.5 59 35151.0 60 32208.0 61 29265.0 62 25694.0 63 22123.0 64 18275.5 65 14428.0 66 11286.5 67 8145.0 68 6363.5 69 4582.0 70 3634.0 71 2686.0 72 2179.0 73 1672.0 74 1403.0 75 925.5 76 717.0 77 609.0 78 501.0 79 358.0 80 215.0 81 165.5 82 116.0 83 100.5 84 85.0 85 60.0 86 35.0 87 21.5 88 8.0 89 7.5 90 7.0 91 4.5 92 2.0 93 3.0 94 4.0 95 4.5 96 5.0 97 5.0 98 5.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1053773.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.421002779618927 #Duplication Level Percentage of deduplicated Percentage of total 1 73.09823157351002 21.50623274309466 2 10.1643245429428 5.980892412617379 3 3.6943376484282213 3.260733546697726 4 1.9510030321019487 2.296018625220655 5 1.2465709815204216 1.8337684156152307 6 0.9136241219945193 1.6127842699636592 7 0.7306561229878294 1.5047645077759362 8 0.6306290767349251 1.4842991855621397 9 0.5308256259305131 1.405567999439512 >10 6.2479752502909385 42.11816419269226 >50 0.736699352761079 13.513415286545834 >100 0.05220441175466582 2.4239651674615814 >500 0.0016212550234300728 0.3618442237064244 >1k 0.0012970040187440584 0.6975494236070063 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT 3101 0.2942759019257468 No Hit ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA 1661 0.15762408032849579 No Hit AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT 1460 0.13854976356387952 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC 1090 0.1034378371812525 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.489709833142433E-5 0.0 0.0 0.34343259886142463 0.0 2 9.489709833142433E-5 0.0 0.0 0.5347451490975761 0.0 3 9.489709833142433E-5 0.0 0.0 0.7345035410852242 0.0 4 9.489709833142433E-5 0.0 0.0 1.6765470362212735 0.0 5 9.489709833142433E-5 0.0 0.0 1.9094245155265888 0.0 6 9.489709833142433E-5 0.0 0.0 3.3279463413847195 0.0 7 9.489709833142433E-5 0.0 0.0 4.520138587722403 0.0 8 9.489709833142433E-5 0.0 0.0 5.686708617510602 0.0 9 9.489709833142433E-5 0.0 0.0 6.95377467443178 0.0 10 9.489709833142433E-5 0.0 0.0 8.587807810600575 0.0 11 9.489709833142433E-5 0.0 0.0 10.070290280734087 0.0 12 9.489709833142433E-5 0.0 0.0 10.765791114405095 0.0 13 9.489709833142433E-5 0.0 0.0 11.135035724012667 0.0 14 9.489709833142433E-5 0.0 0.0 11.499915067096993 0.0 15 9.489709833142433E-5 0.0 0.0 11.781284963649666 0.0 16 9.489709833142433E-5 0.0 0.0 12.24789399614528 0.0 17 9.489709833142433E-5 0.0 0.0 12.885033114342463 0.0 18 9.489709833142433E-5 0.0 0.0 13.567343251345404 0.0 19 9.489709833142433E-5 0.0 0.0 14.008330067291533 0.0 20 9.489709833142433E-5 0.0 0.0 14.428724212899743 0.0 21 9.489709833142433E-5 0.0 0.0 14.931489039859628 0.0 22 9.489709833142433E-5 0.0 0.0 15.537501909804105 0.0 23 9.489709833142433E-5 0.0 0.0 16.09900804063114 0.0 24 9.489709833142433E-5 0.0 0.0 16.521110334009318 0.0 25 9.489709833142433E-5 0.0 0.0 16.90629765613657 0.0 26 9.489709833142433E-5 0.0 0.0 17.267191321090976 0.0 27 9.489709833142433E-5 0.0 0.0 17.646779714416674 0.0 28 9.489709833142433E-5 0.0 0.0 18.03898942182045 0.0 29 9.489709833142433E-5 0.0 0.0 18.48604965205979 0.0 30 9.489709833142433E-5 0.0 0.0 19.05182615231174 0.0 31 9.489709833142433E-5 0.0 0.0 19.49879148545275 0.0 32 9.489709833142433E-5 0.0 0.0 19.9153897471277 0.0 33 9.489709833142433E-5 0.0 0.0 20.31111064716974 0.0 34 9.489709833142433E-5 0.0 0.0 20.727234423353035 0.0 35 9.489709833142433E-5 0.0 0.0 21.192325102275348 0.0 36 9.489709833142433E-5 0.0 0.0 21.637961876039718 0.0 37 9.489709833142433E-5 0.0 0.0 22.07467832256093 0.0 38 9.489709833142433E-5 0.0 0.0 22.5169936978837 0.0 39 9.489709833142433E-5 0.0 0.0 22.955702983469873 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTC 20 7.033018E-4 45.000004 6 TCTTGCG 40 6.8139343E-9 45.000004 1 TACCCGT 20 7.033018E-4 45.000004 10 TTATACG 30 2.1653286E-6 45.000004 1 TCTCGCG 65 0.0 45.000004 1 GCGATAG 30 2.1653286E-6 45.000004 1 CGTACCG 40 6.8139343E-9 45.000004 1 CCGAACG 30 2.1653286E-6 45.000004 1 GCTCGCG 20 7.033018E-4 45.000004 1 ATACCCG 30 2.1653286E-6 45.000004 1 CTATGCG 25 3.8905844E-5 45.0 1 TAATACG 25 3.8905844E-5 45.0 1 CGTCTAC 25 3.8905844E-5 45.0 38 CGATTAG 55 1.8189894E-12 45.0 1 TAACGCG 45 3.8562575E-10 45.0 1 TCTACGA 25 3.8905844E-5 45.0 40 ACGTCTA 25 3.8905844E-5 45.0 37 CTCGACG 45 3.8562575E-10 45.0 1 CAATGCG 25 3.8905844E-5 45.0 1 CCACGCG 55 1.8189894E-12 45.0 1 >>END_MODULE