##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1474437
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869331819535184	33.0	31.0	34.0	30.0	34.0
2	32.072292000268575	34.0	31.0	34.0	30.0	34.0
3	32.231126864016574	34.0	31.0	34.0	30.0	34.0
4	35.820975056920034	37.0	35.0	37.0	35.0	37.0
5	35.64328350414429	37.0	35.0	37.0	33.0	37.0
6	35.65313675660608	37.0	35.0	37.0	33.0	37.0
7	35.8273123911025	37.0	35.0	37.0	35.0	37.0
8	36.00065991290235	37.0	35.0	37.0	35.0	37.0
9	37.635877287398515	39.0	37.0	39.0	35.0	39.0
10	37.10325093578091	39.0	37.0	39.0	33.0	39.0
11	37.07172500418804	39.0	37.0	39.0	33.0	39.0
12	37.104103464576646	39.0	37.0	39.0	33.0	39.0
13	37.0905376085923	39.0	37.0	39.0	33.0	39.0
14	38.30871037555352	40.0	38.0	41.0	34.0	41.0
15	38.289445395089786	40.0	38.0	41.0	33.0	41.0
16	38.23786706383521	40.0	38.0	41.0	33.0	41.0
17	38.22327640991104	40.0	38.0	41.0	33.0	41.0
18	38.165478755619944	40.0	37.0	41.0	33.0	41.0
19	38.18253136620961	40.0	37.0	41.0	34.0	41.0
20	38.122213427905024	40.0	37.0	41.0	33.0	41.0
21	38.12459806692317	40.0	37.0	41.0	33.0	41.0
22	38.111314352529135	40.0	37.0	41.0	33.0	41.0
23	38.02835048225187	40.0	37.0	41.0	33.0	41.0
24	37.930119767748636	40.0	37.0	41.0	33.0	41.0
25	37.91584313198869	40.0	37.0	41.0	33.0	41.0
26	37.83788049268975	40.0	37.0	41.0	33.0	41.0
27	37.72216717296161	40.0	37.0	41.0	33.0	41.0
28	37.647295204881594	40.0	37.0	41.0	32.0	41.0
29	37.58078982011439	40.0	37.0	41.0	32.0	41.0
30	37.400866907165245	39.0	36.0	41.0	31.0	41.0
31	37.38158157995221	39.0	36.0	41.0	31.0	41.0
32	37.304120148911075	39.0	36.0	41.0	31.0	41.0
33	37.188601479751256	39.0	36.0	41.0	31.0	41.0
34	37.139193468422185	39.0	36.0	41.0	31.0	41.0
35	37.0542112006142	39.0	36.0	41.0	31.0	41.0
36	37.08346914788492	39.0	36.0	41.0	31.0	41.0
37	37.05213311928553	39.0	36.0	41.0	31.0	41.0
38	37.10149772421609	39.0	36.0	41.0	31.0	41.0
39	37.12236467207483	39.0	36.0	41.0	31.0	41.0
40	37.054486559954746	39.0	36.0	41.0	31.0	41.0
41	37.038373969182814	39.0	36.0	41.0	31.0	41.0
42	36.913717574911644	39.0	35.0	41.0	31.0	41.0
43	36.858453769133575	39.0	35.0	40.0	31.0	41.0
44	36.796492491710396	39.0	35.0	40.0	30.0	41.0
45	36.63567314168052	39.0	35.0	40.0	30.0	41.0
46	36.49645932650903	39.0	35.0	40.0	30.0	41.0
47	36.42325511364677	39.0	35.0	40.0	30.0	41.0
48	36.41681604571779	39.0	35.0	40.0	30.0	41.0
49	36.305534926212516	39.0	35.0	40.0	30.0	41.0
50	36.15997699460879	38.0	35.0	40.0	29.0	41.0
51	34.96035164608593	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	6.0
12	5.0
13	7.0
14	8.0
15	25.0
16	68.0
17	110.0
18	250.0
19	419.0
20	803.0
21	1364.0
22	1958.0
23	3048.0
24	4086.0
25	5526.0
26	7191.0
27	9958.0
28	13565.0
29	18167.0
30	24390.0
31	32174.0
32	42308.0
33	56798.0
34	80846.0
35	104550.0
36	137646.0
37	202959.0
38	321814.0
39	404251.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.18856960317735	33.472233808565576	22.135838967687327	26.20335762056975
2	20.31338063274321	31.849987486749182	21.009917683834576	26.826714196673034
3	19.998480776052148	30.99630570855181	22.68832103372338	26.316892481672667
4	20.1889263495151	31.17433976494079	20.37937192297806	28.25736196256605
5	18.44704114180531	34.321981881897976	18.575293484903053	28.655683491393663
6	21.107446435486903	35.39208525016667	19.010374807468885	24.490093506877542
7	86.43509353061542	3.7701170005907336	5.366116015808068	4.428673452985784
8	88.3152688110784	3.243339661172366	3.8665605922803077	4.574830935468928
9	80.07978638626133	8.144328987945908	7.4945216377505455	4.281362988042216
10	29.883677634242765	44.455951661549456	12.562150841304174	13.098219862903605
11	19.548749793989163	25.411665605244576	36.207922074663074	18.831662526103184
12	24.024017302875606	23.222491025387995	33.00242736719168	19.75106430454472
13	23.724445330658416	24.257462339862602	30.677539969493438	21.34055235998554
14	18.796259182318405	26.411504865925096	31.917945629416515	22.874290322339984
15	17.72269686666843	31.099056792524877	29.825553753737864	21.352692587068827
16	20.32023070500808	30.118682588676222	29.016227889017976	20.54485881729772
17	19.981389506638806	29.994974352922505	30.120174683625006	19.903461456813684
18	19.500867110632736	29.151262481882917	28.864034204242028	22.483836203242323
19	19.760220341730435	31.72688965347451	29.026401263668777	19.486488741126273
20	21.359203546845336	30.89029914469048	28.27194379956553	19.47855350889865
21	20.97946538237985	30.194643786068852	27.819296450102648	21.006594381448647
22	20.571377413887472	27.594532692817666	29.26981620781356	22.564273685481307
23	20.021947360246656	28.691765060155166	27.756153704770025	23.530133874828156
24	17.820089973325413	30.42056052581426	29.488069005322032	22.2712804955383
25	19.859783768312923	31.01068407805827	26.608868334150593	22.520663819478216
26	21.257876735323382	31.87175850850189	26.05876005553306	20.81160470064167
27	18.724774269772123	29.635922050246975	27.72176769845032	23.917535981530577
28	19.988036111410658	29.66366145179482	28.8742754013905	21.474027035404024
29	20.601015845370128	27.657132858168914	28.899437548026807	22.842413748434147
30	20.22826339816486	28.928736867021108	26.904642246498156	23.93835748831588
31	19.363390907851606	30.001688780192033	27.66045616055484	22.97446415140152
32	20.298052748269342	27.831097564697576	27.209165261045403	24.66168442598768
33	19.375259844944207	28.738698228544184	27.7910822910711	24.09495963544051
34	18.162186651582942	27.051410131460347	29.71737686995104	25.069026347005668
35	18.56430624028019	26.56383419569639	29.214473049713213	25.65738651431021
36	18.024167868820438	27.61786363201683	30.251479039117985	24.10648946004475
37	18.109217280901117	26.751838159243157	29.278497487515576	25.860447072340154
38	19.206720938229303	25.69997904284822	31.134731426300345	23.958568592622136
39	18.447380254293673	25.35659373713492	29.917995818064792	26.278030190506612
40	20.27553567904224	26.41957574314806	27.311984167516144	25.992904410293555
41	18.186127993261156	25.708049920071186	30.431751237930136	25.674070848737518
42	20.034019764832273	27.20631671614318	27.67707267248448	25.08259084654007
43	18.717856375009582	27.177899089618613	29.478980790633987	24.625263744737822
44	21.336143897636862	25.49610461484621	27.81902516011196	25.348726327404968
45	21.18428932534927	26.23781144938712	28.88146458614373	23.696434639119882
46	17.71014970459911	26.71996158533732	30.239677924523058	25.330210785540512
47	19.19844659351332	26.14211390517194	29.018194741450465	25.641244759864275
48	19.769579846409172	24.258750967318374	31.30639016790816	24.6652790183643
49	19.365832517767796	24.788309029141292	29.407360233092362	26.438498219998547
50	18.81660593162	23.96589342237071	28.90737278025443	28.310127865754858
51	18.243912761277695	22.899723758966985	31.99126174939994	26.865101730355384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1362.0
1	1133.0
2	904.0
3	703.5
4	503.0
5	564.0
6	625.0
7	730.0
8	835.0
9	1020.5
10	1206.0
11	1420.5
12	1635.0
13	1997.5
14	2360.0
15	2494.5
16	2629.0
17	2814.5
18	3000.0
19	3429.0
20	3858.0
21	4470.5
22	5083.0
23	6334.0
24	7585.0
25	8594.5
26	13214.0
27	16824.0
28	20241.0
29	23658.0
30	27689.5
31	31721.0
32	37804.5
33	43888.0
34	50436.5
35	56985.0
36	61136.0
37	65287.0
38	69521.5
39	73756.0
40	77910.5
41	82065.0
42	88235.0
43	94405.0
44	107670.0
45	120935.0
46	134663.5
47	148392.0
48	154507.5
49	160623.0
50	150989.0
51	141355.0
52	122291.5
53	103228.0
54	88566.5
55	73905.0
56	63586.5
57	53268.0
58	47195.5
59	41123.0
60	38368.0
61	35613.0
62	31463.5
63	27314.0
64	21586.5
65	15859.0
66	12370.0
67	8881.0
68	6974.5
69	5068.0
70	4154.0
71	3240.0
72	2766.0
73	2292.0
74	1782.5
75	1077.0
76	881.0
77	747.0
78	613.0
79	492.5
80	372.0
81	287.5
82	203.0
83	181.5
84	160.0
85	99.5
86	39.0
87	23.0
88	7.0
89	8.0
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1474437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.137463404463066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99083303293567	20.256394299210385
2	11.566889753442226	6.509258742818788
3	4.48557703603258	3.7863827909779983
4	2.190019443459151	2.464863677815777
5	1.259744639588942	1.7723009347701182
6	0.8596386269412251	1.4512830243972958
7	0.6213920044828913	1.2239076350174312
8	0.5036596501584566	1.1337363979710593
9	0.42488163939145596	1.0759582421644829
>10	4.829402488335469	31.94925108957686
>50	1.1640899724853317	22.20760440981115
>100	0.09779023092685205	3.9450107464631525
>500	0.004135407637433689	0.847989744977995
>1k	0.0017028149095315189	0.9905648227719382
>5k	2.43259272790217E-4	0.3854934412556355
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	5632	0.3819763068886633	TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	4013	0.27217168315770696	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC	3113	0.21113143525291347	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGT	2652	0.1798652638261248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.782249767199277E-5	0.0	0.0	0.43874373744012124	0.0
2	6.782249767199277E-5	0.0	0.0	0.6947058436542219	0.0
3	6.782249767199277E-5	0.0	0.0	1.0372772793954574	0.0
4	6.782249767199277E-5	0.0	0.0	2.5718291117219656	0.0
5	6.782249767199277E-5	0.0	0.0	2.8874071933897483	0.0
6	6.782249767199277E-5	0.0	0.0	4.613150646653604	0.0
7	6.782249767199277E-5	0.0	0.0	5.8741743458689655	0.0
8	6.782249767199277E-5	0.0	0.0	6.911655092757439	0.0
9	6.782249767199277E-5	0.0	0.0	8.452853529855803	0.0
10	6.782249767199277E-5	0.0	0.0	10.372772793954574	0.0
11	6.782249767199277E-5	0.0	0.0	12.393679757086943	0.0
12	6.782249767199277E-5	0.0	0.0	13.301687355919581	0.0
13	6.782249767199277E-5	0.0	0.0	13.732631506127424	0.0
14	6.782249767199277E-5	0.0	0.0	14.214849464575293	0.0
15	6.782249767199277E-5	0.0	0.0	14.596893593961628	0.0
16	6.782249767199277E-5	0.0	0.0	15.250634649021965	0.0
17	6.782249767199277E-5	0.0	0.0	16.162711597714924	0.0
18	6.782249767199277E-5	0.0	0.0	17.122399939773622	0.0
19	6.782249767199277E-5	0.0	0.0	17.71252349201763	0.0
20	1.3564499534398554E-4	0.0	0.0	18.291659799638776	0.0
21	1.3564499534398554E-4	0.0	0.0	18.962153011624096	0.0
22	1.3564499534398554E-4	0.0	0.0	19.744417699772864	0.0
23	1.3564499534398554E-4	0.0	0.0	20.480020509523296	0.0
24	1.3564499534398554E-4	0.0	0.0	21.03901353533586	0.0
25	1.3564499534398554E-4	0.0	0.0	21.52340181370923	0.0
26	1.3564499534398554E-4	0.0	0.0	21.98201754296725	0.0
27	2.034674930159783E-4	0.0	0.0	22.47556185852634	0.0
28	2.034674930159783E-4	0.0	0.0	22.98334889859655	0.0
29	2.034674930159783E-4	0.0	0.0	23.549666754157688	0.0
30	2.034674930159783E-4	0.0	0.0	24.237997283030744	0.0
31	2.034674930159783E-4	0.0	0.0	24.78383274429494	0.0
32	2.712899906879711E-4	0.0	0.0	25.29738469666727	0.0
33	2.712899906879711E-4	0.0	0.0	25.803950931779383	0.0
34	2.712899906879711E-4	0.0	0.0	26.30312451464525	0.0
35	2.712899906879711E-4	0.0	0.0	26.838176198779603	0.0
36	2.712899906879711E-4	0.0	0.0	27.359799028374898	0.0
37	2.712899906879711E-4	0.0	0.0	27.880336698007444	0.0
38	2.712899906879711E-4	0.0	0.0	28.391718330454268	0.0
39	2.712899906879711E-4	0.0	0.0	28.88356708357156	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACG	40	6.8175723E-9	45.000004	1
AACCGCG	40	6.8175723E-9	45.000004	1
CGTATAG	20	7.0339354E-4	45.000004	1
ATCCGTC	20	7.0339354E-4	45.000004	15
ACGTCGG	40	6.8175723E-9	45.000004	2
CGCGTAG	20	7.0339354E-4	45.000004	1
TTACCGT	20	7.0339354E-4	45.000004	28
CGTCCGA	20	7.0339354E-4	45.000004	39
CGTCCCG	20	7.0339354E-4	45.000004	1
ATACCCG	40	6.8175723E-9	45.000004	1
TCAACGA	20	7.0339354E-4	45.000004	19
ATCGACG	40	6.8175723E-9	45.000004	1
CGACGGA	35	1.2122473E-7	45.0	3
TGTGACG	25	3.8913444E-5	45.0	1
TGCGACG	25	3.8913444E-5	45.0	1
GAACGTA	35	1.2122473E-7	45.0	9
ACGTCCG	25	3.8913444E-5	45.0	1
CGTAACG	35	1.2122473E-7	45.0	1
CGCGGAC	30	2.1659216E-6	44.999996	4
TAGCCCG	30	2.1659216E-6	44.999996	1
>>END_MODULE