##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839936
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85698791336483	33.0	31.0	34.0	30.0	34.0
2	32.06967078444072	34.0	31.0	34.0	30.0	34.0
3	32.22216216473636	34.0	31.0	34.0	30.0	34.0
4	35.84094264324901	37.0	35.0	37.0	35.0	37.0
5	35.646244475769585	37.0	35.0	37.0	33.0	37.0
6	35.6459539774459	37.0	35.0	37.0	33.0	37.0
7	35.83145263448644	37.0	35.0	37.0	35.0	37.0
8	36.001869190033524	37.0	35.0	37.0	35.0	37.0
9	37.616387439042974	39.0	37.0	39.0	35.0	39.0
10	37.074191366961294	39.0	37.0	39.0	33.0	39.0
11	37.034705025144774	39.0	37.0	39.0	33.0	39.0
12	37.09299875228589	39.0	37.0	39.0	33.0	39.0
13	37.06629314614447	39.0	37.0	39.0	33.0	39.0
14	38.27002533526364	40.0	38.0	41.0	33.0	41.0
15	38.26147468378543	40.0	38.0	41.0	33.0	41.0
16	38.164039879228895	40.0	37.0	41.0	33.0	41.0
17	38.183337778116424	40.0	37.0	41.0	33.0	41.0
18	38.155533278726	40.0	37.0	41.0	33.0	41.0
19	38.1725893401402	40.0	37.0	41.0	34.0	41.0
20	38.10286021792137	40.0	37.0	41.0	33.0	41.0
21	38.0564721597836	40.0	37.0	41.0	33.0	41.0
22	38.07145544422432	40.0	37.0	41.0	33.0	41.0
23	37.9563276249619	40.0	37.0	41.0	33.0	41.0
24	37.85813204815605	40.0	37.0	41.0	33.0	41.0
25	37.89051903954587	40.0	37.0	41.0	33.0	41.0
26	37.85302094445291	40.0	37.0	41.0	33.0	41.0
27	37.803456453825056	40.0	37.0	41.0	33.0	41.0
28	37.79198177003962	40.0	37.0	41.0	33.0	41.0
29	37.695125580996645	40.0	37.0	41.0	33.0	41.0
30	37.66497685537946	40.0	37.0	41.0	33.0	41.0
31	37.633598274154224	40.0	37.0	41.0	33.0	41.0
32	37.59981236665651	40.0	37.0	41.0	33.0	41.0
33	37.51216759372143	40.0	37.0	41.0	32.0	41.0
34	37.521584977903075	40.0	37.0	41.0	32.0	41.0
35	37.424510915117345	40.0	37.0	41.0	32.0	41.0
36	37.40275330501372	40.0	36.0	41.0	32.0	41.0
37	37.325727198262726	39.0	36.0	41.0	32.0	41.0
38	37.35212206644316	39.0	36.0	41.0	32.0	41.0
39	37.260698434166414	39.0	36.0	41.0	31.0	41.0
40	37.17105231827187	39.0	36.0	41.0	31.0	41.0
41	37.11033102522097	39.0	36.0	41.0	31.0	41.0
42	37.043125904830845	39.0	36.0	41.0	31.0	41.0
43	36.99362927651631	39.0	36.0	41.0	31.0	41.0
44	36.89649092311795	39.0	35.0	41.0	31.0	41.0
45	36.72486117989942	39.0	35.0	40.0	31.0	41.0
46	36.6122275982932	39.0	35.0	40.0	30.0	41.0
47	36.52817119399573	39.0	35.0	40.0	30.0	41.0
48	36.50147630295641	39.0	35.0	40.0	30.0	41.0
49	36.33493147096922	39.0	35.0	40.0	30.0	41.0
50	36.21091249809509	39.0	35.0	40.0	29.0	41.0
51	34.97575291450777	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	3.0
11	3.0
12	3.0
13	7.0
14	16.0
15	14.0
16	31.0
17	90.0
18	134.0
19	260.0
20	504.0
21	946.0
22	1282.0
23	1837.0
24	2441.0
25	3252.0
26	4324.0
27	5741.0
28	7594.0
29	10103.0
30	13411.0
31	17343.0
32	23150.0
33	30422.0
34	44298.0
35	57445.0
36	73830.0
37	109308.0
38	183785.0
39	248286.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.55212778116428	32.82821548308443	22.142520382505335	26.47713635324596
2	21.27543050899116	30.632691062176164	21.759395953977446	26.33248247485523
3	19.791150754343185	32.21959768363304	21.714154411764707	26.275097150259068
4	19.601255333739715	32.2791260286498	20.3309537869552	27.788664850655287
5	18.424141839378237	33.3263486741847	18.782264362999086	29.467245123437973
6	20.517277506857663	35.54663688661993	20.05724245656812	23.87884314995428
7	87.49988094330998	3.443238532459616	4.80703291679366	4.249847607436757
8	89.41538402925937	3.223459882657726	3.53824577110637	3.8229103169765315
9	80.76758229198416	8.183837816214568	7.417946129228893	3.630633762572386
10	29.314257276744893	46.57235789393477	11.766372676013411	12.347012153306919
11	19.260753200243826	26.067819452910694	36.810542708015845	17.860884638829624
12	24.06611932337702	22.66934623590369	34.402859265467846	18.861675175251445
13	23.958253962206644	23.753238341968913	30.871995009143554	21.41651268668089
14	18.908702567814693	27.21004933709235	31.855284212130446	22.02596388296251
15	18.430451843950017	30.15265448796099	30.628643134715027	20.788250533373972
16	20.899092311795183	29.878347874123744	28.644206225236207	20.578353588844863
17	20.080934737884792	29.60642239408717	30.290284021639746	20.022358846388297
18	20.450605760438894	28.668255676622984	28.768739523011277	22.112399039926853
19	20.574543774763793	30.41410297927461	30.311237999085645	18.700115246875953
20	22.738161002743066	29.33711616123133	29.04828463121	18.876438204815603
21	22.608984494056692	28.986494209082597	27.7954510819872	20.609070214873515
22	21.301622980798538	27.049560918927156	28.544912945748248	23.10390315452606
23	20.47394087168546	28.259057832977753	28.41061699939043	22.85638429594636
24	18.72368847150259	28.79100312404755	29.69238132429137	22.79292708015849
25	21.0941071700701	29.277111589454435	26.798589416336483	22.830191824138982
26	22.010605569948186	29.70714435385553	26.469516725083814	21.812733351112463
27	19.003471693081377	28.81779087930509	28.86946148277964	23.309275944833892
28	21.096250190490704	27.844383381590976	28.592416565071627	22.466949862846693
29	20.69169555775678	27.57162450472417	27.348988494361475	24.387691443157575
30	21.629028878390734	28.08404449862847	27.684014020115814	22.60291260286498
31	20.932547241694603	28.437047584577872	25.30288021944529	25.327524954282232
32	21.408893058518743	27.850455272782686	26.519163364827797	24.22148830387077
33	21.118513791526976	28.39942567052728	26.794065262115208	23.687995275830538
34	20.5589473483694	25.63873914202987	28.41537926699177	25.38693424260896
35	20.392267982322462	27.570672051203903	27.506143325205727	24.530916641267904
36	19.000971502590673	28.598488456263333	29.463197195976836	22.937342845169155
37	19.008829244132887	29.582611056080466	27.724969521487353	23.683590178299298
38	19.7162640963121	27.724493294727214	29.130076577263033	23.429166031697655
39	19.664474436147515	28.30620428223103	28.26262953367876	23.7666917479427
40	21.33317300365742	28.795289164888754	28.151787755257544	21.71975007619628
41	19.613161002743066	27.275173346540686	30.936166565071627	22.17549908564462
42	21.395082482474855	28.403116427918317	27.292674679975615	22.90912640963121
43	19.195391077415422	27.065395458701612	29.315685957025295	24.423527506857663
44	22.057156735751295	25.403959349283756	27.97808404449863	24.560799870466322
45	21.759991237427613	26.727393515696434	27.233265391648885	24.279349855227064
46	18.935847493142337	27.580791869856753	29.443552842121306	24.039807794879607
47	19.512081872904602	27.65782154830844	28.579677499238038	24.25041907954892
48	20.375600045717768	24.63199577110637	31.36381819567205	23.628585987503808
49	20.10355550899116	26.321172089302042	28.418236627552574	25.157035774154224
50	19.028116427918317	24.677118256629075	28.54800841968912	27.746756895763486
51	19.322543622371228	23.910274116123134	30.740318309966476	26.026863951539163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	908.0
1	759.0
2	610.0
3	434.0
4	258.0
5	267.0
6	276.0
7	302.0
8	328.0
9	485.0
10	642.0
11	779.0
12	916.0
13	935.0
14	954.0
15	1092.5
16	1231.0
17	1479.0
18	1727.0
19	1870.0
20	2013.0
21	2410.0
22	2807.0
23	3699.0
24	4591.0
25	4975.5
26	7012.5
27	8665.0
28	12351.0
29	16037.0
30	17368.5
31	18700.0
32	22532.0
33	26364.0
34	28784.5
35	31205.0
36	34472.5
37	37740.0
38	39136.5
39	40533.0
40	44071.0
41	47609.0
42	49898.5
43	52188.0
44	57087.5
45	61987.0
46	71144.5
47	80302.0
48	85181.5
49	90061.0
50	85317.5
51	80574.0
52	70168.5
53	59763.0
54	52142.5
55	44522.0
56	38151.0
57	31780.0
58	29661.0
59	27542.0
60	24690.5
61	21839.0
62	18893.5
63	15948.0
64	12941.0
65	9934.0
66	7843.0
67	5752.0
68	4328.5
69	2905.0
70	2398.0
71	1891.0
72	1636.5
73	1382.0
74	1198.5
75	738.5
76	462.0
77	333.0
78	204.0
79	148.5
80	93.0
81	120.0
82	147.0
83	139.0
84	131.0
85	74.5
86	18.0
87	13.0
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	839936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.46153160582413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60772772787466	20.665271375539778
2	11.436711222565984	6.510126358554906
3	4.200809923132284	3.5868445319186733
4	2.0272316670867205	2.3079247266046496
5	1.272177292912401	1.8104057115219043
6	0.8313625733693252	1.4197111294710199
7	0.6036092075974961	1.2025749777721817
8	0.5013388114339583	1.1415096341483126
9	0.4061653084677298	1.040407808772924
>10	4.892282468370792	32.89967349743193
>50	1.0891621500674278	21.083534727750415
>100	0.12680299498594208	5.005048013251372
>500	0.0037788972015678104	0.6749560951922962
>1k	8.397549336817357E-4	0.6520114120696119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	4422	0.5264686833282536	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1034	0.12310461749466625	Illumina PCR Primer Index 7 (95% over 22bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	949	0.11298479884181653	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6064747790307833	0.0
2	0.0	0.0	0.0	0.8931632886315147	0.0
3	0.0	0.0	0.0	1.1925908640658336	0.0
4	0.0	0.0	0.0	2.5525754343188054	0.0
5	0.0	0.0	0.0	2.759495961597074	0.0
6	0.0	0.0	0.0	4.088763905821396	0.0
7	0.0	0.0	0.0	5.102174451386772	0.0
8	0.0	0.0	0.0	6.0291498399878085	0.0
9	0.0	0.0	0.0	7.315676432490094	0.0
10	0.0	0.0	0.0	9.185104579396526	0.0
11	0.0	0.0	0.0	10.886067509905516	0.0
12	0.0	0.0	0.0	11.746252095397745	0.0
13	0.0	0.0	0.0	12.09889801127705	0.0
14	0.0	0.0	0.0	12.461782802499238	0.0
15	0.0	0.0	0.0	12.761210377933557	0.0
16	0.0	0.0	0.0	13.410188395306308	0.0
17	0.0	0.0	0.0	14.30358979731789	0.0
18	0.0	0.0	0.0	15.321405440414507	0.0
19	0.0	0.0	0.0	15.850255257543433	0.0
20	0.0	0.0	0.0	16.441728893629993	0.0
21	0.0	0.0	0.0	17.072848407497712	0.0
22	0.0	0.0	0.0	17.87017106065224	0.0
23	0.0	0.0	0.0	18.572962701920147	0.0
24	0.0	0.0	0.0	19.081096654983238	0.0
25	0.0	0.0	0.0	19.530773773239865	0.0
26	0.0	0.0	0.0	19.957830120390124	0.0
27	0.0	0.0	0.0	20.449653306918623	0.0
28	0.0	0.0	0.0	20.90230684242609	0.0
29	0.0	0.0	0.0	21.415679289850654	0.0
30	0.0	0.0	0.0	22.05799013258153	0.0
31	0.0	0.0	0.0	22.54576539164889	0.0
32	0.0	0.0	0.0	23.02425422889363	0.0
33	0.0	0.0	0.0	23.506552880219445	0.0
34	1.1905669003352636E-4	0.0	0.0	23.96885000761963	0.0
35	1.1905669003352636E-4	0.0	0.0	24.47531716702225	0.0
36	1.1905669003352636E-4	0.0	0.0	24.96321148277964	0.0
37	1.1905669003352636E-4	0.0	0.0	25.466702224931424	0.0
38	1.1905669003352636E-4	0.0	0.0	25.93042803261201	0.0
39	1.1905669003352636E-4	0.0	0.0	26.410702720207254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	95	0.0	45.000004	1
TTCGTTA	70	0.0	45.000004	20
ACGTCCG	35	1.2114106E-7	45.000004	1
TACTACG	70	0.0	45.000004	1
CGGGTCG	25	3.8899052E-5	45.0	18
AGCCGAC	25	3.8899052E-5	45.0	10
TCGCACG	40	6.8121153E-9	45.0	1
CGAGTTA	25	3.8899052E-5	45.0	32
CATTGCG	20	7.032201E-4	45.0	1
TCGGGTA	25	3.8899052E-5	45.0	5
CGCGAAG	20	7.032201E-4	45.0	1
TGCGGCG	25	3.8899052E-5	45.0	17
CCGCTAG	20	7.032201E-4	45.0	1
TTATCCG	20	7.032201E-4	45.0	1
CGTTAAG	25	3.8899052E-5	45.0	1
CACTCCG	75	0.0	45.0	1
ACGGCAC	20	7.032201E-4	45.0	39
GTTCGCA	20	7.032201E-4	45.0	28
CACTACG	40	6.8121153E-9	45.0	1
TACGAAG	20	7.032201E-4	45.0	1
>>END_MODULE