##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	850237
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.840578568093367	33.0	31.0	34.0	30.0	34.0
2	32.03852925713654	33.0	31.0	34.0	30.0	34.0
3	32.19749316955155	34.0	31.0	34.0	30.0	34.0
4	35.795492315671986	37.0	35.0	37.0	35.0	37.0
5	35.624109512994615	37.0	35.0	37.0	33.0	37.0
6	35.6294527290626	37.0	35.0	37.0	33.0	37.0
7	35.83988817235665	37.0	35.0	37.0	35.0	37.0
8	36.003005044475834	37.0	35.0	37.0	35.0	37.0
9	37.60624508225354	39.0	37.0	39.0	35.0	39.0
10	37.05948694305235	39.0	37.0	39.0	33.0	39.0
11	37.02826976478323	39.0	37.0	39.0	33.0	39.0
12	37.08149139592843	39.0	37.0	39.0	33.0	39.0
13	37.054638883040845	39.0	37.0	39.0	33.0	39.0
14	38.247870887764236	40.0	38.0	41.0	33.0	41.0
15	38.2305321927886	40.0	38.0	41.0	33.0	41.0
16	38.169500974434186	40.0	37.0	41.0	33.0	41.0
17	38.156414035145495	40.0	37.0	41.0	33.0	41.0
18	38.115846522793056	40.0	37.0	41.0	33.0	41.0
19	38.13240190676247	40.0	37.0	41.0	33.0	41.0
20	38.057487500543964	40.0	37.0	41.0	33.0	41.0
21	38.035524212660704	40.0	37.0	41.0	33.0	41.0
22	38.032953164823454	40.0	37.0	41.0	33.0	41.0
23	37.94219258865469	40.0	37.0	41.0	33.0	41.0
24	37.8649799996942	40.0	37.0	41.0	33.0	41.0
25	37.88731142022753	40.0	37.0	41.0	33.0	41.0
26	37.83398981695692	40.0	37.0	41.0	33.0	41.0
27	37.764743242178355	40.0	37.0	41.0	33.0	41.0
28	37.75989282988155	40.0	37.0	41.0	33.0	41.0
29	37.652928536396324	40.0	37.0	41.0	33.0	41.0
30	37.6440510116591	40.0	37.0	41.0	33.0	41.0
31	37.61270680998357	40.0	37.0	41.0	33.0	41.0
32	37.58463228488057	40.0	37.0	41.0	32.0	41.0
33	37.49903379881139	40.0	37.0	41.0	32.0	41.0
34	37.487605220662005	40.0	37.0	41.0	32.0	41.0
35	37.39632714172637	40.0	36.0	41.0	32.0	41.0
36	37.390104170954686	39.0	36.0	41.0	32.0	41.0
37	37.282474180728435	39.0	36.0	41.0	31.0	41.0
38	37.283603277674345	39.0	36.0	41.0	31.0	41.0
39	37.21079887137351	39.0	36.0	41.0	31.0	41.0
40	37.132613612439826	39.0	36.0	41.0	31.0	41.0
41	37.10040729820039	39.0	36.0	41.0	31.0	41.0
42	37.01985916867885	39.0	36.0	41.0	31.0	41.0
43	36.97899879680607	39.0	36.0	41.0	31.0	41.0
44	36.93128033712953	39.0	35.0	41.0	31.0	41.0
45	36.76077728915585	39.0	35.0	40.0	31.0	41.0
46	36.62527271807743	39.0	35.0	40.0	30.0	41.0
47	36.533867615735375	39.0	35.0	40.0	30.0	41.0
48	36.516438357775534	39.0	35.0	40.0	30.0	41.0
49	36.35818483552233	39.0	35.0	40.0	30.0	41.0
50	36.198609328928285	39.0	35.0	40.0	29.0	41.0
51	35.00761905209959	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	6.0
12	2.0
13	9.0
14	11.0
15	30.0
16	34.0
17	62.0
18	146.0
19	307.0
20	517.0
21	945.0
22	1438.0
23	1868.0
24	2456.0
25	3285.0
26	4311.0
27	5669.0
28	7669.0
29	10197.0
30	13795.0
31	18023.0
32	23532.0
33	31213.0
34	44949.0
35	58792.0
36	74422.0
37	112024.0
38	186480.0
39	247975.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.663384444572515	32.72534599176465	22.15840994922592	26.452859614436914
2	21.224317455015484	30.509140392619937	21.81768142294443	26.448860729420147
3	19.789776262383313	32.345569529437086	21.721590568276845	26.14306363990276
4	19.727087859032245	32.07964367582215	20.427363194027077	27.765905271118523
5	18.460382222839044	33.295539949449385	18.7941715074738	29.44990632023777
6	20.510869322318364	35.48892838114549	20.16743566793729	23.83276662859885
7	87.62239234472271	3.4642105671712713	4.720095690966166	4.193301397139856
8	89.53138948316763	3.212280811115018	3.513255715759253	3.743073989958094
9	80.89403307548366	8.182071587098656	7.331249992649108	3.592645344768576
10	29.354638765426582	46.55866540740993	11.743549151589498	12.343146675573987
11	19.282153093784437	26.15894156570462	36.72587760824335	17.833027732267592
12	24.152206972879327	22.72672207866748	34.25303768243443	18.86803326601877
13	24.104102738412937	23.61459216665471	30.8891520834779	21.392153011454454
14	18.85627183949887	27.29086125397977	31.847355502054132	22.005511404467228
15	18.51942458396894	30.135950329143522	30.59476357768481	20.749861509202727
16	20.972622927489628	29.816157142067446	28.658362315448514	20.55285761499441
17	20.209659189143732	29.61480152004676	30.149240741111004	20.026298549698495
18	20.45629630326603	28.745984943021767	28.620608136319635	22.17711061739256
19	20.568500312265876	30.494085766674466	30.262503278497643	18.674910642562015
20	22.75212675995046	29.288186705589148	29.060955945224688	18.898730589235708
21	22.77788428402904	28.97004011822586	27.752614859151038	20.499460738594063
22	21.41967474951102	26.993532391556705	28.52910423799482	23.057688620937455
23	20.526276791059434	28.357152182273882	28.347978269588364	22.76859275707832
24	18.763003727196065	28.76021626911085	29.63326695968301	22.843513044010084
25	21.078946223229522	29.3405250536027	26.748424262881997	22.83210446028578
26	21.90142277976611	29.77722682028658	26.444861844403384	21.876488555543926
27	19.06033258961913	28.799381819422116	28.799734662217713	23.340550928741045
28	21.073888809825966	27.922802700893985	28.513461540723352	22.489846948556696
29	20.746803538307553	27.567254777197412	27.366604840767927	24.3193368437271
30	21.5978603612875	28.075230788591888	27.80001340802623	22.52689544209438
31	20.883941771529585	28.47006187686492	25.345168464792756	25.300827886812737
32	21.484597823900863	27.95891028031008	26.487202979875025	24.069288915914033
33	21.13528345625984	28.489585844887955	26.772417572982594	23.60271312586961
34	20.63789272873328	25.666961094377218	28.38961371946881	25.305532457420693
35	20.51945516367789	27.622063024780147	27.432821672074965	24.425660139466995
36	19.05586324754157	28.632957634165535	29.36051947868653	22.950659639606368
37	18.96553549186874	29.660435854943973	27.661346189356617	23.712682463830674
38	19.68239443825663	27.836473830237924	29.144344459250775	23.33678727225468
39	19.739672585408538	28.33951004249403	28.144505590794093	23.77631178130333
40	21.2811251451066	28.89664881674168	28.084639929807807	21.73758610834391
41	19.669927326145533	27.33332000371661	30.847869476393054	22.148883193744805
42	21.25501477823242	28.53921906480193	27.306621565516437	22.899144591449208
43	19.198764579758347	27.120202955176026	29.339231296685515	24.341801168380112
44	22.113481299919904	25.410679610508602	27.990548517648612	24.48529057192289
45	21.675603390584037	26.77394655843018	27.39130383645972	24.159146214526068
46	18.973298033371872	27.641351764272788	29.38180766068755	24.003542541667795
47	19.523262337442386	27.647702934593532	28.55909587562056	24.26993885234352
48	20.366321390388798	24.779796691981176	31.201182729050842	23.652699188579184
49	20.11462686286294	26.422397519750373	28.28952397978446	25.173451637602223
50	19.00975845558356	24.84295555239304	28.583206799986353	27.564079192037045
51	19.304852646967845	24.056586575272544	30.68473849056204	25.95382228719757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	940.0
1	765.0
2	590.0
3	426.0
4	262.0
5	274.5
6	287.0
7	298.0
8	309.0
9	489.5
10	670.0
11	798.5
12	927.0
13	972.0
14	1017.0
15	1177.5
16	1338.0
17	1525.5
18	1713.0
19	1884.5
20	2056.0
21	2427.5
22	2799.0
23	3731.5
24	4664.0
25	5092.0
26	7134.0
27	8748.0
28	12375.5
29	16003.0
30	17528.5
31	19054.0
32	23036.0
33	27018.0
34	29281.5
35	31545.0
36	34797.5
37	38050.0
38	39517.5
39	40985.0
40	44733.0
41	48481.0
42	50627.5
43	52774.0
44	57775.5
45	62777.0
46	71876.5
47	80976.0
48	86045.0
49	91114.0
50	86090.5
51	81067.0
52	70905.0
53	60743.0
54	52745.0
55	44747.0
56	38523.0
57	32299.0
58	30054.0
59	27809.0
60	24950.0
61	22091.0
62	19189.0
63	16287.0
64	13195.0
65	10103.0
66	8042.5
67	5982.0
68	4463.0
69	2944.0
70	2476.5
71	2009.0
72	1707.5
73	1406.0
74	1218.5
75	756.0
76	481.0
77	338.5
78	196.0
79	146.0
80	96.0
81	127.5
82	159.0
83	145.0
84	131.0
85	72.0
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	3.0
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	850237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.151112187806827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54315424244409	20.42170473536418
2	11.439475529464103	6.440679179992297
3	4.228914892015812	3.5714597267347234
4	2.059956541332862	2.319602707882717
5	1.2637690560808017	1.7788252238604692
6	0.8159881222749882	1.3782583904448615
7	0.6008843935434269	1.1840894782180296
8	0.49016111372022186	1.103886440195064
9	0.39986791582752806	1.0131053902888714
>10	4.906267876660462	32.8290479989705
>50	1.1076834669117048	21.20328563910217
>100	0.1396699242934693	5.475949220865081
>500	0.0037862328874735665	0.739474608224438
>1k	4.206925430526184E-4	0.5406312598565918
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	4565	0.5369091206334233	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	992	0.11667335107740547	Illumina PCR Primer Index 7 (95% over 22bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	974	0.11455629430382352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3522853039799493E-4	0.0	0.0	0.601949809288469	0.0
2	2.3522853039799493E-4	0.0	0.0	0.8765791185281281	0.0
3	2.3522853039799493E-4	0.0	0.0	1.1766131090507705	0.0
4	2.3522853039799493E-4	0.0	0.0	2.5140049186285705	0.0
5	2.3522853039799493E-4	0.0	0.0	2.7135963266712695	0.0
6	2.3522853039799493E-4	0.0	0.0	4.040167623850762	0.0
7	2.3522853039799493E-4	0.0	0.0	5.040241720837837	0.0
8	2.3522853039799493E-4	0.0	0.0	5.950576133478077	0.0
9	2.3522853039799493E-4	0.0	0.0	7.219869283505658	0.0
10	2.3522853039799493E-4	0.0	0.0	9.065589947273525	0.0
11	2.3522853039799493E-4	0.0	0.0	10.741828454889637	0.0
12	2.3522853039799493E-4	0.0	0.0	11.596648934355951	0.0
13	2.3522853039799493E-4	0.0	0.0	11.948786044361748	0.0
14	2.3522853039799493E-4	0.0	0.0	12.318800522677794	0.0
15	2.3522853039799493E-4	0.0	0.0	12.625891369112377	0.0
16	2.3522853039799493E-4	0.0	0.0	13.265830586060122	0.0
17	2.3522853039799493E-4	0.0	0.0	14.138646048101883	0.0
18	2.3522853039799493E-4	0.0	0.0	15.124371204734679	0.0
19	2.3522853039799493E-4	0.0	0.0	15.660339411246511	0.0
20	2.3522853039799493E-4	0.0	0.0	16.263112520391374	0.0
21	2.3522853039799493E-4	0.0	0.0	16.88293969799009	0.0
22	2.3522853039799493E-4	0.0	0.0	17.66919106084539	0.0
23	2.3522853039799493E-4	0.0	0.0	18.351824256060368	0.0
24	2.3522853039799493E-4	0.0	0.0	18.85733036788566	0.0
25	2.3522853039799493E-4	0.0	0.0	19.317790216139734	0.0
26	2.3522853039799493E-4	0.0	0.0	19.738731671286946	0.0
27	2.3522853039799493E-4	0.0	0.0	20.21942117315525	0.0
28	2.3522853039799493E-4	0.0	0.0	20.678822493022533	0.0
29	2.3522853039799493E-4	0.0	0.0	21.16492225108999	0.0
30	2.3522853039799493E-4	0.0	0.0	21.807213753341717	0.0
31	2.3522853039799493E-4	0.0	0.0	22.303545952481485	0.0
32	2.3522853039799493E-4	0.0	0.0	22.77906042668103	0.0
33	2.3522853039799493E-4	0.0	0.0	23.253163529698192	0.0
34	2.3522853039799493E-4	0.0	0.0	23.71174154970908	0.0
35	2.3522853039799493E-4	0.0	0.0	24.213248776517606	0.0
36	2.3522853039799493E-4	0.0	0.0	24.699230920319863	0.0
37	2.3522853039799493E-4	0.0	0.0	25.209088759957517	0.0
38	2.3522853039799493E-4	0.0	0.0	25.690366333151815	0.0
39	2.3522853039799493E-4	0.0	0.0	26.15788303731783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	7.0322497E-4	45.0	38
AACCGAA	35	1.2114288E-7	45.0	29
TTGGCCG	25	3.889946E-5	45.0	26
CTCAACG	50	2.1827873E-11	45.0	1
TTTACCG	20	7.0322497E-4	45.0	1
CGAAACG	55	1.8189894E-12	45.0	1
CGTGCAC	20	7.0322497E-4	45.0	18
TTTCGCG	20	7.0322497E-4	45.0	1
TAGCGCG	45	3.8380676E-10	45.0	1
TTCCGCA	40	6.8121153E-9	45.0	34
CCGTCAG	35	1.2114288E-7	45.0	1
ATCCGCG	25	3.889946E-5	45.0	1
CACTCCG	95	0.0	45.0	1
CGCTCGT	20	7.0322497E-4	45.0	45
CGCTACG	25	3.889946E-5	45.0	1
TCGCTAT	20	7.0322497E-4	45.0	19
ACGTGCG	20	7.0322497E-4	45.0	1
AGCGACG	20	7.0322497E-4	45.0	1
CGTACCG	40	6.8121153E-9	45.0	1
TTACGTC	20	7.0322497E-4	45.0	34
>>END_MODULE