FastQCFastQC Report
Sat 18 Jun 2016
SRR3554090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218909
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT48920.4013425120333019No Hit
AATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCT19790.16235830566514808No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC18100.14849344782916526No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGT15980.13110084510000336No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA15430.12658861325989063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA207.033454E-445.0000049
TTCGTAA207.033454E-445.00000431
TAACGCG850.045.0000041
CGTTCCG207.033454E-445.0000041
ATCGTAG207.033454E-445.0000041
CATACCG406.8157533E-945.0000041
TAGGCGT207.033454E-445.00000430
CGACTCG207.033454E-445.0000041
CGACTAG406.8157533E-945.0000041
ATCGCGC207.033454E-445.0000041
ACGACCG207.033454E-445.0000041
TGCGTGA207.033454E-445.00000431
CGAACCG502.1827873E-1145.01
GTATTCG253.890945E-545.028
GATCGCG253.890945E-545.013
ATACGCA351.2120108E-745.016
TCGAACG253.890945E-545.01
CAATGCG302.1656106E-644.9999961
CGCAATG1400.043.3928571
TATACGG1250.043.1999972