##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218909
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.931831662576943	33.0	31.0	34.0	30.0	34.0
2	32.141044163264034	34.0	31.0	34.0	30.0	34.0
3	32.28123182288424	34.0	31.0	34.0	30.0	34.0
4	35.88465750929725	37.0	35.0	37.0	35.0	37.0
5	35.68669523319625	37.0	35.0	37.0	33.0	37.0
6	35.69478197305951	37.0	35.0	37.0	33.0	37.0
7	35.83852445096394	37.0	35.0	37.0	35.0	37.0
8	36.01600037410504	37.0	35.0	37.0	35.0	37.0
9	37.66623513322159	39.0	37.0	39.0	35.0	39.0
10	37.123268431031356	39.0	37.0	39.0	33.0	39.0
11	37.08975649535773	39.0	37.0	39.0	33.0	39.0
12	37.071404017855315	39.0	37.0	39.0	33.0	39.0
13	37.04869272439534	39.0	37.0	39.0	33.0	39.0
14	38.272592129519104	40.0	38.0	41.0	33.0	41.0
15	38.270699453363626	40.0	38.0	41.0	33.0	41.0
16	38.19914776246627	40.0	37.0	41.0	33.0	41.0
17	38.220906564805084	40.0	37.0	41.0	33.0	41.0
18	38.18486449767784	40.0	37.0	41.0	33.0	41.0
19	38.18664149661706	40.0	37.0	41.0	34.0	41.0
20	38.11933786689573	40.0	37.0	41.0	33.0	41.0
21	38.09421376000998	40.0	37.0	41.0	33.0	41.0
22	38.099025439963114	40.0	37.0	41.0	33.0	41.0
23	37.99183532158676	40.0	37.0	41.0	33.0	41.0
24	37.89383210723688	40.0	37.0	41.0	33.0	41.0
25	37.91559665241622	40.0	37.0	41.0	33.0	41.0
26	37.82535365642554	40.0	37.0	41.0	33.0	41.0
27	37.805232384041794	40.0	37.0	41.0	33.0	41.0
28	37.77566495940222	40.0	37.0	41.0	33.0	41.0
29	37.63380695359539	40.0	37.0	41.0	32.0	41.0
30	37.65695880496411	40.0	37.0	41.0	33.0	41.0
31	37.60217046555567	40.0	37.0	41.0	33.0	41.0
32	37.561285543055305	40.0	37.0	41.0	32.0	41.0
33	37.52171244941173	40.0	36.0	41.0	32.0	41.0
34	37.47172348386959	40.0	36.0	41.0	32.0	41.0
35	37.41749219999196	40.0	36.0	41.0	32.0	41.0
36	37.37111137911034	39.0	36.0	41.0	32.0	41.0
37	37.32959064212341	39.0	36.0	41.0	32.0	41.0
38	37.32510958570328	39.0	36.0	41.0	32.0	41.0
39	37.25256602420689	39.0	36.0	41.0	32.0	41.0
40	37.15246667306583	39.0	36.0	41.0	31.0	41.0
41	37.12150701980214	39.0	36.0	41.0	31.0	41.0
42	37.10727626098421	39.0	35.0	41.0	31.0	41.0
43	37.05212858384014	39.0	35.0	41.0	31.0	41.0
44	36.935548921207406	39.0	35.0	41.0	31.0	41.0
45	36.7626246093843	39.0	35.0	40.0	31.0	41.0
46	36.65825832773406	39.0	35.0	40.0	31.0	41.0
47	36.59373587363782	39.0	35.0	40.0	30.0	41.0
48	36.57020581520032	39.0	35.0	40.0	31.0	41.0
49	36.42725092685344	39.0	35.0	40.0	30.0	41.0
50	36.29122928783035	38.0	35.0	40.0	30.0	41.0
51	35.06953677427929	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	7.0
12	6.0
13	8.0
14	28.0
15	40.0
16	63.0
17	152.0
18	219.0
19	425.0
20	684.0
21	1093.0
22	1676.0
23	2410.0
24	3278.0
25	4357.0
26	6026.0
27	8062.0
28	10751.0
29	14412.0
30	19129.0
31	25128.0
32	33010.0
33	44725.0
34	66887.0
35	85383.0
36	105763.0
37	157004.0
38	263309.0
39	364754.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.30365597431802	32.102560568508395	21.708593504519204	26.885189952654382
2	22.60464070738669	30.226784772284066	21.154819596869004	26.01375492346024
3	20.774233351300218	30.696220964813612	21.606206862038103	26.923338821848063
4	20.71893800111411	31.11725321578559	19.769892584270032	28.393916198830265
5	18.990753206350924	33.93862872453973	18.051142456081624	29.01947561302772
6	21.151127770818	36.14043378135693	18.96302349067896	23.745414957146103
7	87.43597758323222	3.7761637661219996	4.675902795040483	4.1119558556052995
8	89.19025128208915	3.149373743240882	3.574261901421681	4.08611307324829
9	81.3061516487285	7.691140191761649	6.750873116861062	4.251835042648795
10	32.06252476599976	43.2352210050135	12.027887233583474	12.674366995403267
11	23.172443554030693	26.858116561613706	32.364352055813846	17.605087828541755
12	28.534861913399606	22.97029556759364	29.921183615840068	18.573658903166685
13	25.253895081585252	24.155453770543986	28.58769604621838	22.00295510165238
14	18.628954253352795	27.249450123019848	29.780812185323104	24.340783438304253
15	18.05007592855578	30.888277960044597	30.250330418431563	20.811315692968055
16	21.05374560365048	27.024248733908767	31.34220848315994	20.579797179280813
17	19.626239530596624	28.42148183334441	30.099375753235062	21.8529028828239
18	20.398733621623926	28.874920112986285	27.426001448836622	23.300344816553164
19	21.687016832265577	31.35935496415237	27.020064664384297	19.933563539197756
20	25.64186497925604	28.332303724067998	26.905371935066526	19.12045936160944
21	23.51734214777313	28.823972913482464	26.15322390760918	21.50546103113522
22	21.58897834046676	27.280543502427168	27.421899419891066	23.708578737215003
23	21.700553527785914	29.4656122811465	26.343476010104116	22.49035818096347
24	20.462233029701153	27.5834373197671	28.810272136804304	23.14405751372744
25	21.55952577263766	28.656446051345917	25.33191567212975	24.45211250388667
26	23.036748436511665	30.219483160760973	25.340283811178686	21.403484591548672
27	20.05309666267129	29.11095085851364	27.172414019422288	23.663538459392786
28	21.371324684615505	28.951217851373645	27.210398807458148	22.467058656552705
29	22.311673800095004	29.07641177479205	26.093744487898608	22.518169937214346
30	22.431124882989625	26.452754061213756	26.297287164177146	24.818833891619473
31	22.564358783141316	29.015947868134536	23.800874388490033	24.618818960234112
32	22.089671993561456	27.967797431965796	26.585249596155254	23.357280978317497
33	22.872175035215918	28.642417112352113	25.16496309404558	23.320444758386394
34	20.365999430638382	27.506073053853896	27.32205603535621	24.805871480151513
35	20.75208239499421	27.450859744246696	27.01743936585914	24.779618494899946
36	19.139656857074648	31.333922384689917	25.451448795603284	24.07497196263216
37	21.136934750666374	29.727731930767597	26.22558369820881	22.90974962035722
38	22.123226590336113	26.61699930019386	28.839970826370138	22.41980328309989
39	21.036927284973693	26.542506454542547	27.515261598691943	24.905304661791817
40	22.379521358854518	26.486226617409503	28.539948429292096	22.594303594443886
41	18.621406520092968	26.416328044177213	31.218491290161936	23.74377414556788
42	21.60973460693128	27.807900343667985	27.062561684260267	23.519803365140465
43	21.474859895201366	27.618878849856713	27.227955491345128	23.67830576359679
44	23.007706071577125	26.3882701661896	26.24207385456995	24.361949907663327
45	23.37557602741468	25.458832447705284	26.672868934432348	24.492722590447688
46	18.563567911960615	27.856222244646645	28.187091899395277	25.393117943997463
47	20.131035212636874	27.925546533826562	27.386457889801452	24.55696036373511
48	20.66790876103138	24.76033895885583	29.63756933454425	24.934182945568537
49	20.82296545517344	25.811771018181012	27.33838211055952	26.026881416086024
50	19.063933402739664	25.531848562936197	27.824718662344765	27.579499371979367
51	18.815760651533463	24.704797486932986	29.947354560512725	26.53208730102083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	816.0
1	653.5
2	491.0
3	396.5
4	302.0
5	330.5
6	359.0
7	386.5
8	414.0
9	511.0
10	608.0
11	727.0
12	846.0
13	958.5
14	1071.0
15	1214.0
16	1357.0
17	1600.5
18	1844.0
19	2171.5
20	2499.0
21	3081.0
22	3663.0
23	4359.0
24	5055.0
25	6013.0
26	9581.0
27	12191.0
28	14378.5
29	16566.0
30	21111.5
31	25657.0
32	29852.0
33	34047.0
34	38469.0
35	42891.0
36	48298.5
37	53706.0
38	56136.0
39	58566.0
40	60955.5
41	63345.0
42	67873.0
43	72401.0
44	79995.5
45	87590.0
46	94878.5
47	102167.0
48	108716.0
49	115265.0
50	110948.0
51	106631.0
52	96498.0
53	86365.0
54	78169.0
55	69973.0
56	62174.0
57	54375.0
58	52616.0
59	50857.0
60	49758.0
61	48659.0
62	43870.5
63	39082.0
64	30203.5
65	21325.0
66	16389.5
67	11454.0
68	9585.0
69	7716.0
70	6275.5
71	4835.0
72	3781.5
73	2728.0
74	2498.0
75	1672.5
76	1077.0
77	773.5
78	470.0
79	315.0
80	160.0
81	129.5
82	99.0
83	80.5
84	62.0
85	48.0
86	34.0
87	23.5
88	13.0
89	16.5
90	20.0
91	14.5
92	9.0
93	8.0
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1218909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.081834001124534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81440651210951	19.71947670051351
2	10.67590100389609	5.782459575999053
3	4.004378736122285	3.2533776062788977
4	1.9798347567027703	2.144702249227248
5	1.182034733243505	1.600583421463206
6	0.8242084577713459	1.339264598141187
7	0.6210966908124192	1.1774306235460794
8	0.5067514885450635	1.0979007754080137
9	0.44062739336165074	1.0739698131031583
>10	5.695184721753735	36.48223229416789
>50	1.14500476673826	20.745875992279757
>100	0.1069155079039858	4.2149270472482305
>500	0.0021322181062253774	0.3925792703206498
>1k	0.001523012933018127	0.9752200323031568
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT	4892	0.4013425120333019	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCT	1979	0.16235830566514808	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC	1810	0.14849344782916526	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGT	1598	0.13110084510000336	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1543	0.12658861325989063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.204057891114102E-5	0.0	0.0	0.44720319564462974	0.0
2	8.204057891114102E-5	0.0	0.0	0.6386859068232329	0.0
3	8.204057891114102E-5	0.0	0.0	0.8852998870301229	0.0
4	8.204057891114102E-5	0.0	0.0	1.9722555170238303	0.0
5	8.204057891114102E-5	0.0	0.0	2.1506117355766508	0.0
6	8.204057891114102E-5	0.0	0.0	3.2777672492368177	0.0
7	8.204057891114102E-5	0.0	0.0	4.1678254898437865	0.0
8	8.204057891114102E-5	0.0	0.0	5.015550791732607	0.0
9	8.204057891114102E-5	0.0	0.0	6.25534802023777	0.0
10	8.204057891114102E-5	0.0	0.0	7.851201361217285	0.0
11	8.204057891114102E-5	0.0	0.0	9.392907920115448	0.0
12	8.204057891114102E-5	0.0	0.0	10.096652006015216	0.0
13	8.204057891114102E-5	0.0	0.0	10.40463233924764	0.0
14	8.204057891114102E-5	0.0	0.0	10.740260347573116	0.0
15	8.204057891114102E-5	0.0	0.0	11.027566454919933	0.0
16	8.204057891114102E-5	0.0	0.0	11.553856768634903	0.0
17	8.204057891114102E-5	0.0	0.0	12.256288205272092	0.0
18	8.204057891114102E-5	0.0	0.0	12.986941601054713	0.0
19	8.204057891114102E-5	0.0	0.0	13.429468483701408	0.0
20	8.204057891114102E-5	0.0	0.0	13.867401093929079	0.0
21	8.204057891114102E-5	0.0	0.0	14.413709308898367	0.0
22	8.204057891114102E-5	0.0	0.0	15.071182508292251	0.0
23	8.204057891114102E-5	0.0	0.0	15.704781899222994	0.0
24	8.204057891114102E-5	0.0	0.0	16.144683483344533	0.0
25	8.204057891114102E-5	0.0	0.0	16.542662331642475	0.0
26	8.204057891114102E-5	0.0	0.0	16.91118861211132	0.0
27	8.204057891114102E-5	0.0	0.0	17.298912388045373	0.0
28	8.204057891114102E-5	0.0	0.0	17.702716117446013	0.0
29	8.204057891114102E-5	0.0	0.0	18.13457772483426	0.0
30	8.204057891114102E-5	0.0	0.0	18.67030270512401	0.0
31	8.204057891114102E-5	0.0	0.0	19.12341282245024	0.0
32	8.204057891114102E-5	0.0	0.0	19.59867389608248	0.0
33	8.204057891114102E-5	0.0	0.0	20.018311457212967	0.0
34	8.204057891114102E-5	0.0	0.0	20.438851464711476	0.0
35	8.204057891114102E-5	0.0	0.0	20.88695710672413	0.0
36	8.204057891114102E-5	0.0	0.0	21.341461913891848	0.0
37	8.204057891114102E-5	0.0	0.0	21.828536830887295	0.0
38	8.204057891114102E-5	0.0	0.0	22.283944084423037	0.0
39	8.204057891114102E-5	0.0	0.0	22.762568821790634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCA	20	7.033454E-4	45.000004	9
TTCGTAA	20	7.033454E-4	45.000004	31
TAACGCG	85	0.0	45.000004	1
CGTTCCG	20	7.033454E-4	45.000004	1
ATCGTAG	20	7.033454E-4	45.000004	1
CATACCG	40	6.8157533E-9	45.000004	1
TAGGCGT	20	7.033454E-4	45.000004	30
CGACTCG	20	7.033454E-4	45.000004	1
CGACTAG	40	6.8157533E-9	45.000004	1
ATCGCGC	20	7.033454E-4	45.000004	1
ACGACCG	20	7.033454E-4	45.000004	1
TGCGTGA	20	7.033454E-4	45.000004	31
CGAACCG	50	2.1827873E-11	45.0	1
GTATTCG	25	3.890945E-5	45.0	28
GATCGCG	25	3.890945E-5	45.0	13
ATACGCA	35	1.2120108E-7	45.0	16
TCGAACG	25	3.890945E-5	45.0	1
CAATGCG	30	2.1656106E-6	44.999996	1
CGCAATG	140	0.0	43.392857	1
TATACGG	125	0.0	43.199997	2
>>END_MODULE