FastQCFastQC Report
Sat 18 Jun 2016
SRR3554089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232414
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT48870.3965388254271698No Hit
AATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCT19470.1579826259682217No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC16510.13396472289344327No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16130.13088134344465416No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGT15540.12609399114258685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG650.045.0000041
CGTTACG302.1656306E-645.0000041
CTTCGCG207.0334855E-445.01
CGTATAG253.8909708E-545.01
TTTCGCG253.8909708E-545.01
CAATGCG406.8157533E-945.01
CCGAACG700.045.01
TTGCACG351.212029E-745.01
TAGTGCG351.212029E-745.01
TATCGCG351.212029E-745.01
GCGTACG253.8909708E-545.01
TCGAACG453.8562575E-1045.01
TACCGCG351.212029E-745.01
TCACGCG1100.042.9545441
ACCGCGC1000.042.7535
CGTAGCG900.042.51
TATAGCG750.042.01
ACACGAC2200.041.9318226
ATTACCG650.041.5384641
CTCGATC650.041.53846416