FastQCFastQC Report
Sat 18 Jun 2016
SRR3554087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186587
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT45960.38732937407876544Illumina Single End Adapter 2 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT15400.12978399392543488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG900.045.0000041
CGCGACG551.8189894E-1245.0000041
TTATCCG453.8562575E-1045.0000041
TTCTACG406.8157533E-945.01
GGTCGAC207.0333795E-445.08
CGTGCCG253.8908824E-545.01
GCGAAGT207.0333795E-445.035
CGTCTAG253.8908824E-545.01
CGCTACA207.0333795E-445.031
AACGCCG253.8908824E-545.01
ACGTGCG351.2119744E-745.01
GCTAGCG502.1827873E-1145.01
CCGAACG351.2119744E-745.01
CGATAGT207.0333795E-445.041
ATCGCCC207.0333795E-445.010
CGCCAAT207.0333795E-445.013
AAACGCG1050.042.8571431
TAAACCG1000.042.751
CGACAAG850.042.3529431
ATACGCG650.041.5384641