##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186587
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.927067294686356	33.0	31.0	34.0	30.0	34.0
2	32.123830785269014	34.0	31.0	34.0	30.0	34.0
3	32.27730710011149	34.0	31.0	34.0	30.0	34.0
4	35.8588843464491	37.0	35.0	37.0	35.0	37.0
5	35.69747856667905	37.0	35.0	37.0	33.0	37.0
6	35.71629808855145	37.0	35.0	37.0	33.0	37.0
7	35.923060845938814	37.0	35.0	37.0	35.0	37.0
8	36.08211113049443	37.0	35.0	37.0	35.0	37.0
9	37.72617094237506	39.0	38.0	39.0	35.0	39.0
10	37.156036599086285	39.0	37.0	39.0	34.0	39.0
11	37.10612538313668	39.0	37.0	39.0	33.0	39.0
12	37.130327569744146	39.0	37.0	39.0	33.0	39.0
13	37.10715691306242	39.0	37.0	39.0	33.0	39.0
14	38.31997653775071	40.0	38.0	41.0	34.0	41.0
15	38.29843745127833	40.0	38.0	41.0	34.0	41.0
16	38.23990487001796	40.0	38.0	41.0	33.0	41.0
17	38.22143340522018	40.0	38.0	41.0	33.0	41.0
18	38.16754523688529	40.0	37.0	41.0	33.0	41.0
19	38.20295688390316	40.0	37.0	41.0	34.0	41.0
20	38.12804455130555	40.0	37.0	41.0	33.0	41.0
21	38.12337064201782	40.0	37.0	41.0	33.0	41.0
22	38.13070512318102	40.0	37.0	41.0	34.0	41.0
23	38.03703310418874	40.0	37.0	41.0	33.0	41.0
24	37.96506366579105	40.0	37.0	41.0	33.0	41.0
25	37.993434109761864	40.0	37.0	41.0	33.0	41.0
26	37.91941341005759	40.0	37.0	41.0	33.0	41.0
27	37.86116820764091	40.0	37.0	41.0	33.0	41.0
28	37.87444662717525	40.0	37.0	41.0	33.0	41.0
29	37.75252383516759	40.0	37.0	41.0	33.0	41.0
30	37.761938231246425	40.0	37.0	41.0	33.0	41.0
31	37.700058234246626	40.0	37.0	41.0	33.0	41.0
32	37.63608146726705	40.0	37.0	41.0	33.0	41.0
33	37.594012069911436	40.0	37.0	41.0	33.0	41.0
34	37.5159259287351	40.0	37.0	41.0	32.0	41.0
35	37.403070318484865	40.0	36.0	41.0	32.0	41.0
36	37.41114810797691	39.0	36.0	41.0	32.0	41.0
37	37.34109930413867	39.0	36.0	41.0	32.0	41.0
38	37.34772587260774	39.0	36.0	41.0	32.0	41.0
39	37.309311495912226	39.0	36.0	41.0	32.0	41.0
40	37.20221863209356	39.0	36.0	41.0	31.0	41.0
41	37.13893039448435	39.0	36.0	41.0	31.0	41.0
42	37.03480486470861	39.0	36.0	41.0	31.0	41.0
43	36.9790348284618	39.0	35.0	41.0	31.0	41.0
44	36.94393247187101	39.0	35.0	41.0	31.0	41.0
45	36.80848433363925	39.0	35.0	40.0	31.0	41.0
46	36.669595234062065	39.0	35.0	40.0	31.0	41.0
47	36.5640134267441	39.0	35.0	40.0	30.0	41.0
48	36.4983166004684	39.0	35.0	40.0	30.0	41.0
49	36.370122881845155	39.0	35.0	40.0	30.0	41.0
50	36.20865473833777	38.0	35.0	40.0	29.0	41.0
51	35.025895277800956	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	6.0
12	10.0
13	7.0
14	10.0
15	24.0
16	42.0
17	77.0
18	191.0
19	371.0
20	612.0
21	1021.0
22	1393.0
23	2195.0
24	3038.0
25	4264.0
26	5816.0
27	7938.0
28	10449.0
29	14084.0
30	18768.0
31	24543.0
32	32287.0
33	43095.0
34	64077.0
35	80916.0
36	101255.0
37	152057.0
38	258787.0
39	359145.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.319948726894868	32.5622141486465	21.768736721369777	26.34910040308886
2	21.517596265591987	30.16171591295034	21.866411818096775	26.4542760033609
3	20.362350169014157	30.8341486970614	22.02307963933534	26.780421494589103
4	20.177787216613698	31.244148132416754	20.582814408045934	27.995250242923614
5	18.929669716590524	33.48477608468659	17.838725689730296	29.746828508992596
6	21.008741879019404	35.3729646456602	19.60311380455036	24.01517967077003
7	89.20020192366847	2.991942436584928	4.07782994420131	3.7300256955452906
8	90.86295400168719	2.6450652164569473	3.0393894421563696	3.4525913396994907
9	82.68403412476287	7.737232920974189	6.142238200823033	3.436494753439908
10	31.60771186604943	46.27785404694304	11.104031984169724	11.010402102837803
11	20.698608698730055	25.055052853267394	36.06031416154062	18.186024286461926
12	24.5732508446494	23.107618741820026	32.978449957735926	19.340680455794647
13	24.23395840338719	23.90123943714199	31.08689038393308	20.77791177553774
14	19.961621018939194	27.44324689213686	30.87131411350369	21.72381797542026
15	19.452008154480033	30.143680994313943	30.33127785826071	20.073032992945315
16	22.706046838537755	28.922868698207548	29.520380722188932	18.850703741065765
17	21.47377309881197	28.734850457657128	29.739412280768285	20.051964162762612
18	21.58611210134613	28.261054604508562	28.828227513026857	21.324605781118454
19	21.606759554925176	30.546432752086446	28.88494480387869	18.96186288910969
20	24.542996004507046	29.22095050763239	28.0179203042002	18.218133183660363
21	23.284006988109596	29.058467689263406	27.575222044401293	20.0823032782257
22	22.642756072668924	26.06551394882971	29.652945801698483	21.63878417680288
23	21.639289828727264	28.73206937207301	28.13388314552578	21.49475765367394
24	20.19160836921355	28.106072289684615	29.438802211721516	22.263517129380315
25	21.671483001246433	29.09133506434842	26.90953128594869	22.327650648456455
26	22.77077028485901	30.512722623794126	26.375984230401983	20.34052286094488
27	20.307486935218407	28.865982856714258	28.720439377812163	22.106090830255177
28	21.619990780279913	28.551214533784712	28.459101608225946	21.36969307770943
29	22.131204875832957	28.478400656673298	28.142816329523246	21.2475781379705
30	23.591190532173368	27.831166193460742	26.536023064469777	22.041620209896116
31	23.78266406087375	28.59486914992327	25.04814227696747	22.574324512235513
32	22.95002389205343	29.130775914450435	27.122495021435427	20.796705172060708
33	23.62270950212669	27.92875701486701	26.631338452216312	21.81719503078999
34	22.266803866888814	28.053653040190056	28.34415007074913	21.335393022171996
35	22.070020992982396	28.599251466601267	28.504357455458383	20.82637008495795
36	21.41916269097841	31.02646497896909	27.402626187544616	20.151746142507882
37	22.042547238424152	28.91477826741739	27.57775030402322	21.464924190135235
38	23.1529588643732	26.518409522437043	29.18445929375596	21.144172319433803
39	23.59919668764279	25.268185139395595	28.786005577340728	22.346612595620886
40	25.108567681931454	26.103774944441493	28.151749513520713	20.63590786010634
41	21.853517694024966	26.1924325818503	30.370297331759073	21.583752392365668
42	23.25737598675866	27.203652155299192	28.104976710515118	21.433995147427034
43	21.269068344756853	27.47459731144872	28.455730595396712	22.800603748397716
44	23.45576009175897	26.585408402418025	26.93641511326182	23.022416392561183
45	23.703445259386797	25.526657548076965	27.890496019255224	22.879401173281018
46	20.823167622770182	27.106314159855117	28.997536632375038	23.072981584999667
47	22.659948238098007	26.868152103469868	28.16287385585718	22.309025802574947
48	22.428612482691957	25.852718763984434	30.05291647388687	21.665752279436738
49	22.047098105743615	25.07822856646837	29.022566402632087	23.85210692515593
50	20.55542492880842	25.252341379098205	28.41831235299224	25.773921339101136
51	20.820217986544602	24.796496169265296	30.370887259004185	24.012398585185917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	617.0
1	516.0
2	415.0
3	328.0
4	241.0
5	256.5
6	272.0
7	297.0
8	322.0
9	435.0
10	548.0
11	619.5
12	691.0
13	875.5
14	1060.0
15	1080.5
16	1101.0
17	1491.5
18	1882.0
19	2245.5
20	2609.0
21	3158.0
22	3707.0
23	4729.5
24	5752.0
25	7615.5
26	11791.0
27	14103.0
28	15976.5
29	17850.0
30	21791.5
31	25733.0
32	30222.5
33	34712.0
34	38853.5
35	42995.0
36	47290.5
37	51586.0
38	55009.5
39	58433.0
40	62928.5
41	67424.0
42	71103.5
43	74783.0
44	83177.0
45	91571.0
46	97470.5
47	103370.0
48	111250.5
49	119131.0
50	115222.5
51	111314.0
52	98640.5
53	85967.0
54	75098.0
55	64229.0
56	57377.5
57	50526.0
58	47592.0
59	44658.0
60	40850.5
61	37043.0
62	32393.0
63	27743.0
64	21765.0
65	15787.0
66	12360.5
67	8934.0
68	6753.0
69	4572.0
70	3362.5
71	2153.0
72	1613.0
73	1073.0
74	1044.5
75	852.0
76	688.0
77	473.0
78	258.0
79	172.5
80	87.0
81	73.0
82	59.0
83	41.0
84	23.0
85	20.5
86	18.0
87	17.5
88	17.0
89	18.5
90	20.0
91	16.5
92	13.0
93	7.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1186587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.350688191926725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.09497638017433	19.991979073703973
2	10.401730596738131	5.689889804156171
3	3.581026618005873	2.9383062730820564
4	1.83789428774511	2.0107069437535903
5	1.1324255464439266	1.5486309010680037
6	0.8380334077209382	1.3752474257395908
7	0.6392238758162043	1.2238249038598725
8	0.5389450588529245	1.1792414605812749
9	0.4890469116905569	1.2038192633585831
>10	6.354129818667096	40.50716366346578
>50	1.017764064102501	18.29350132949252
>100	0.07169955711133866	3.0138773259883154
>500	0.001862326158658041	0.32531062793257004
>1k	0.001241550772438694	0.6985010038176374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	4596	0.38732937407876544	Illumina Single End Adapter 2 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1540	0.12978399392543488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.427532073080188E-5	0.0	0.0	0.4460692726281343	0.0
2	8.427532073080188E-5	0.0	0.0	0.6488356943064436	0.0
3	8.427532073080188E-5	0.0	0.0	0.8831210859380728	0.0
4	8.427532073080188E-5	0.0	0.0	1.957041498010681	0.0
5	8.427532073080188E-5	0.0	0.0	2.1426157542599067	0.0
6	8.427532073080188E-5	0.0	0.0	3.224710872443403	0.0
7	8.427532073080188E-5	0.0	0.0	4.059205098319803	0.0
8	8.427532073080188E-5	0.0	0.0	4.838836090400451	0.0
9	8.427532073080188E-5	0.0	0.0	5.991891028639282	0.0
10	8.427532073080188E-5	0.0	0.0	7.53547780314465	0.0
11	8.427532073080188E-5	0.0	0.0	8.897029884871484	0.0
12	8.427532073080188E-5	0.0	0.0	9.569041292378898	0.0
13	8.427532073080188E-5	0.0	0.0	9.853301949203892	0.0
14	8.427532073080188E-5	0.0	0.0	10.162255275003012	0.0
15	8.427532073080188E-5	0.0	0.0	10.421401886250228	0.0
16	8.427532073080188E-5	0.0	0.0	10.909271717960841	0.0
17	8.427532073080188E-5	0.0	0.0	11.626538972700695	0.0
18	8.427532073080188E-5	0.0	0.0	12.35113818034413	0.0
19	8.427532073080188E-5	0.0	0.0	12.812966937948923	0.0
20	8.427532073080188E-5	0.0	0.0	13.24867034612717	0.0
21	8.427532073080188E-5	0.0	0.0	13.761822774057022	0.0
22	8.427532073080188E-5	0.0	0.0	14.413692379909774	0.0
23	8.427532073080188E-5	0.0	0.0	15.027722366754396	0.0
24	8.427532073080188E-5	0.0	0.0	15.47244323425084	0.0
25	8.427532073080188E-5	0.0	0.0	15.871402602590456	0.0
26	8.427532073080188E-5	0.0	0.0	16.244489447465714	0.0
27	8.427532073080188E-5	0.0	0.0	16.628110707432324	0.0
28	8.427532073080188E-5	0.0	0.0	17.037941592146215	0.0
29	8.427532073080188E-5	0.0	0.0	17.475920433984193	0.0
30	1.6855064146160375E-4	0.0	0.0	18.001124232778547	0.0
31	1.6855064146160375E-4	0.0	0.0	18.436996191598258	0.0
32	1.6855064146160375E-4	0.0	0.0	18.875143584077694	0.0
33	1.6855064146160375E-4	0.0	0.0	19.300144026523128	0.0
34	1.6855064146160375E-4	0.0	0.0	19.730032437570948	0.0
35	1.6855064146160375E-4	0.0	0.0	20.18284373585755	0.0
36	1.6855064146160375E-4	0.0	0.0	20.634306629012453	0.0
37	1.6855064146160375E-4	0.0	0.0	21.07944887311255	0.0
38	2.528259621924056E-4	0.0	0.0	21.53478843102107	0.0
39	2.528259621924056E-4	0.0	0.0	22.006814502434292	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGCG	90	0.0	45.000004	1
CGCGACG	55	1.8189894E-12	45.000004	1
TTATCCG	45	3.8562575E-10	45.000004	1
TTCTACG	40	6.8157533E-9	45.0	1
GGTCGAC	20	7.0333795E-4	45.0	8
CGTGCCG	25	3.8908824E-5	45.0	1
GCGAAGT	20	7.0333795E-4	45.0	35
CGTCTAG	25	3.8908824E-5	45.0	1
CGCTACA	20	7.0333795E-4	45.0	31
AACGCCG	25	3.8908824E-5	45.0	1
ACGTGCG	35	1.2119744E-7	45.0	1
GCTAGCG	50	2.1827873E-11	45.0	1
CCGAACG	35	1.2119744E-7	45.0	1
CGATAGT	20	7.0333795E-4	45.0	41
ATCGCCC	20	7.0333795E-4	45.0	10
CGCCAAT	20	7.0333795E-4	45.0	13
AAACGCG	105	0.0	42.857143	1
TAAACCG	100	0.0	42.75	1
CGACAAG	85	0.0	42.352943	1
ATACGCG	65	0.0	41.538464	1
>>END_MODULE