FastQCFastQC Report
Sat 18 Jun 2016
SRR3554086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880682
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT39080.4437470051619086No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT14070.15976254766192566No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCC12070.13705287493101936No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT11800.13398706911234703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG453.8380676E-1045.01
CGAGTCC207.032388E-445.013
CGTTGCG406.8121153E-945.01
CCGGACC207.032388E-445.05
TAACGCG750.045.01
GTTACGG1100.045.02
ACGATAG207.032388E-445.01
CCGTAAC253.8900605E-545.023
CGCTCAG453.8380676E-1045.01
TATCTCG453.8380676E-1045.01
TCGTCCA207.032388E-445.028
CTTGACG207.032388E-445.01
TTGCGAA253.8900605E-545.024
ATAAGCG253.8900605E-545.01
AGCGAAT207.032388E-445.035
TCGTAAG253.8900605E-545.01
CATAACG551.8189894E-1245.01
ATGCGAT253.8900605E-545.012
ATACGAG207.032388E-445.01
TATCGTC207.032388E-445.015