##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	880682
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.982076390797133	33.0	31.0	34.0	30.0	34.0
2	32.19007314785587	34.0	31.0	34.0	30.0	34.0
3	32.32551590699026	34.0	31.0	34.0	30.0	34.0
4	35.91892646835066	37.0	35.0	37.0	35.0	37.0
5	35.72911561721484	37.0	35.0	37.0	33.0	37.0
6	35.73262653261904	37.0	35.0	37.0	33.0	37.0
7	35.86336157659632	37.0	35.0	37.0	35.0	37.0
8	36.03477872830375	37.0	35.0	37.0	35.0	37.0
9	37.6490674272893	39.0	37.0	39.0	35.0	39.0
10	37.18027392407248	39.0	37.0	39.0	34.0	39.0
11	37.12626237393293	39.0	37.0	39.0	34.0	39.0
12	37.138917339062225	39.0	37.0	39.0	33.0	39.0
13	37.14116219021167	39.0	37.0	39.0	33.0	39.0
14	38.376543406132974	40.0	38.0	41.0	34.0	41.0
15	38.357365087511724	40.0	38.0	41.0	34.0	41.0
16	38.287421566467806	40.0	38.0	41.0	33.0	41.0
17	38.29391085545066	40.0	38.0	41.0	33.0	41.0
18	38.254041754004284	40.0	38.0	41.0	33.0	41.0
19	38.27809016194268	40.0	38.0	41.0	34.0	41.0
20	38.20815799573512	40.0	37.0	41.0	34.0	41.0
21	38.19450039855476	40.0	37.0	41.0	34.0	41.0
22	38.20385224178534	40.0	37.0	41.0	34.0	41.0
23	38.12156147167763	40.0	37.0	41.0	33.0	41.0
24	38.028262187713615	40.0	37.0	41.0	33.0	41.0
25	38.00335535414599	40.0	37.0	41.0	33.0	41.0
26	37.95093915851579	40.0	37.0	41.0	33.0	41.0
27	37.91073395391299	40.0	37.0	41.0	33.0	41.0
28	37.869222943128165	40.0	37.0	41.0	33.0	41.0
29	37.80372143407041	40.0	37.0	41.0	33.0	41.0
30	37.76943891211584	40.0	37.0	41.0	33.0	41.0
31	37.732430093949915	40.0	37.0	41.0	33.0	41.0
32	37.71438839444885	40.0	37.0	41.0	33.0	41.0
33	37.675816015315405	40.0	37.0	41.0	33.0	41.0
34	37.64349106715023	40.0	37.0	41.0	33.0	41.0
35	37.565975005734195	40.0	37.0	41.0	32.0	41.0
36	37.53894254679896	40.0	37.0	41.0	32.0	41.0
37	37.504652076458925	40.0	37.0	41.0	32.0	41.0
38	37.50589543104094	40.0	37.0	41.0	32.0	41.0
39	37.454970125425525	40.0	36.0	41.0	32.0	41.0
40	37.35077360500158	39.0	36.0	41.0	32.0	41.0
41	37.2713238149525	39.0	36.0	41.0	32.0	41.0
42	37.2041894804254	39.0	36.0	41.0	31.0	41.0
43	37.13118015356281	39.0	36.0	41.0	31.0	41.0
44	37.03976350146818	39.0	36.0	41.0	31.0	41.0
45	36.904475168108355	39.0	35.0	41.0	31.0	41.0
46	36.793778003865185	39.0	35.0	41.0	31.0	41.0
47	36.70003701676655	39.0	35.0	40.0	31.0	41.0
48	36.66161906340768	39.0	35.0	40.0	31.0	41.0
49	36.50070059340375	39.0	35.0	40.0	30.0	41.0
50	36.39170551913176	39.0	35.0	40.0	30.0	41.0
51	35.20313688709432	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	8.0
12	10.0
13	7.0
14	21.0
15	36.0
16	48.0
17	77.0
18	140.0
19	298.0
20	550.0
21	898.0
22	1310.0
23	1792.0
24	2349.0
25	3140.0
26	4059.0
27	5501.0
28	7293.0
29	9907.0
30	13050.0
31	17362.0
32	23043.0
33	30470.0
34	45192.0
35	57697.0
36	73069.0
37	110749.0
38	191911.0
39	280602.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.918633513572438	33.18802927731008	22.324743778117416	25.568593431000064
2	21.904274187504683	30.92591877658451	21.489254918347374	25.680552117563433
3	20.095335206124346	31.28166579991416	22.142952847906507	26.480046146054992
4	20.383066759624928	32.28883978552985	19.754008824978822	27.574084629866398
5	19.309807626362296	33.62496338065272	17.865245343949347	29.199983649035634
6	20.4625506141831	36.841788522985595	18.576171648790368	24.119489214040936
7	88.30258822140115	3.66000440567651	4.263627506863998	3.77377986605835
8	90.08098269295841	2.8808355342791154	3.180262569236115	3.8579192035263583
9	81.63718572651649	8.416772455892138	6.392999970477425	3.553041847113941
10	30.781939451470564	45.65904605748727	11.244013162526315	12.315001328515855
11	20.62923961202795	26.732918351913632	34.79632830011287	17.84151373594555
12	24.81747100542534	24.194431134052927	32.30541784662341	18.68268001389832
13	24.68836651595014	25.124278684019885	29.24642492977034	20.940929870259637
14	19.620248852593786	27.81412587063208	30.140164100095152	22.425461176678983
15	18.814282567373922	30.802491705292034	29.970182199704322	20.413043527629725
16	22.242080569376917	29.030342393735765	29.115163021385698	19.61241401550162
17	21.438271703066487	29.266409441773533	28.743519227144414	20.55179962801556
18	20.995660181541123	28.390043171087857	28.568995392207402	22.04530125516361
19	21.564764580177634	30.701547210003156	28.877052102802146	18.85663610701706
20	23.203608112803487	29.346688134877287	28.232438042335374	19.217265709983852
21	22.757817237095797	29.540855836726536	28.00409228302611	19.697234643151557
22	21.997951587519673	26.609264183893842	29.15467785193748	22.238106376649007
23	21.20754142812048	29.07723786792509	28.09708839285917	21.618132311095266
24	19.45424114493086	29.53529196690747	28.94461337917659	22.065853508985082
25	20.942519547350802	30.66384915326985	26.450410023141156	21.943221276238187
26	21.82297355912804	31.021526498781625	26.512066784605565	20.64343315748477
27	19.55370951149223	29.658491941472633	28.0840303310389	22.703768215996238
28	20.04537392611635	29.88184157278109	28.53935926929357	21.533425231808984
29	21.99942771624718	27.824685868451947	28.01942131211947	22.156465103181397
30	20.601079617841627	28.35200446926359	28.198373533239014	22.84854237965577
31	21.356062687780604	29.794409332767106	26.70055706827209	22.1489709111802
32	22.286023786111215	27.656974935334205	27.437372400026344	22.619628878528232
33	21.139753055018723	28.453857351461707	28.283648354343566	22.122741239176
34	20.784687321871004	27.27772340072807	28.85207146279815	23.085517814602774
35	21.793450984577863	27.54399431349795	29.05657206573996	21.60598263618423
36	20.124857780674525	28.84332823879675	29.447859726893476	21.58395425363525
37	21.458937505251612	27.209367285808046	28.87160178134673	22.460093427593613
38	21.072305327007932	26.615736440622157	31.594150896691424	20.717807335678483
39	20.776171194596916	26.371266813673948	30.45174080996319	22.400821181765952
40	22.66777338471775	26.544768713338073	28.648479246765575	22.1389786551786
41	20.61902025929904	27.038590546871628	29.85334093350381	22.48904826032552
42	23.292743578272294	27.790621359355587	26.451091313323083	22.465543749049033
43	21.429414930701434	27.327571132372412	28.621227639488488	22.62178629743767
44	21.84409355476778	26.120324929997434	27.63119945678463	24.404382058450157
45	21.567603289268998	26.77118415046521	28.694125688954696	22.9670868713111
46	19.609802403137568	27.09093634251637	30.531451761248668	22.767809493097396
47	20.570989301473176	27.100928598517964	28.73659277696149	23.591489323047366
48	21.361285912508716	25.224996082581452	30.773082679105514	22.640635325804322
49	21.464047181616067	25.609925035370317	28.976520469363514	23.949507313650102
50	20.195371314503987	25.06307611601009	29.28412298650364	25.45742958298228
51	19.805219136987017	24.29548917770546	30.97633425004712	24.9229574352604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	703.0
1	595.5
2	488.0
3	357.0
4	226.0
5	234.5
6	243.0
7	284.5
8	326.0
9	417.0
10	508.0
11	520.5
12	533.0
13	752.0
14	971.0
15	999.0
16	1027.0
17	1300.0
18	1573.0
19	1692.0
20	1811.0
21	2244.5
22	2678.0
23	3530.0
24	4382.0
25	5865.0
26	9329.0
27	11310.0
28	13191.5
29	15073.0
30	18000.0
31	20927.0
32	24132.0
33	27337.0
34	31853.0
35	36369.0
36	38415.0
37	40461.0
38	43184.5
39	45908.0
40	48343.5
41	50779.0
42	53273.5
43	55768.0
44	62677.0
45	69586.0
46	74181.0
47	78776.0
48	84168.0
49	89560.0
50	82841.5
51	76123.0
52	68239.5
53	60356.0
54	53626.5
55	46897.0
56	40699.0
57	34501.0
58	30995.5
59	27490.0
60	25800.0
61	24110.0
62	22036.0
63	19962.0
64	16122.5
65	12283.0
66	9229.5
67	6176.0
68	4713.0
69	3250.0
70	2762.0
71	2274.0
72	1627.5
73	981.0
74	818.5
75	532.5
76	409.0
77	362.0
78	315.0
79	186.0
80	57.0
81	38.5
82	20.0
83	22.0
84	24.0
85	50.0
86	76.0
87	44.0
88	12.0
89	10.5
90	9.0
91	9.0
92	9.0
93	12.5
94	16.0
95	9.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	880682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.229681790297892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15685476666404	19.188810213086356
2	11.016912617953711	5.779402245608871
3	4.106031720287269	3.230997163320136
4	1.9997712545515898	2.09813454641092
5	1.184802848961377	1.553850085624765
6	0.7490354633419278	1.1788177111864266
7	0.5658061501202907	1.038864069085412
8	0.45981678517076907	0.9648678365493631
9	0.363601232185885	0.8583430156916385
>10	4.778292632916267	30.46821887236505
>50	1.4147379516312864	25.689611032594996
>100	0.20129972688758793	6.869612006362276
>500	0.0013015068548766463	0.20403210799263796
>1k	0.0017353424731688617	0.8764390941211921
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT	3908	0.4437470051619086	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT	1407	0.15976254766192566	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCC	1207	0.13705287493101936	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT	1180	0.13398706911234703	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2709672730906276E-4	0.0	0.0	0.5040411862624647	0.0
2	2.2709672730906276E-4	0.0	0.0	0.702183080839622	0.0
3	2.2709672730906276E-4	0.0	0.0	0.9346165812404478	0.0
4	2.2709672730906276E-4	0.0	0.0	2.0581776396020355	0.0
5	2.2709672730906276E-4	0.0	0.0	2.216350510172798	0.0
6	2.2709672730906276E-4	0.0	0.0	3.2580431983394686	0.0
7	2.2709672730906276E-4	0.0	0.0	3.9875914348198327	0.0
8	2.2709672730906276E-4	0.0	0.0	4.6926132247508185	0.0
9	2.2709672730906276E-4	0.0	0.0	5.711369143459273	0.0
10	2.2709672730906276E-4	0.0	0.0	7.0730411204044135	0.0
11	2.2709672730906276E-4	0.0	0.0	8.519760821726798	0.0
12	2.2709672730906276E-4	0.0	0.0	9.188787780379297	0.0
13	2.2709672730906276E-4	0.0	0.0	9.47254514115197	0.0
14	2.2709672730906276E-4	0.0	0.0	9.793659913566986	0.0
15	2.2709672730906276E-4	0.0	0.0	10.063110180519189	0.0
16	2.2709672730906276E-4	0.0	0.0	10.610072648243067	0.0
17	2.2709672730906276E-4	0.0	0.0	11.349840237452337	0.0
18	2.2709672730906276E-4	0.0	0.0	12.178516195403109	0.0
19	2.2709672730906276E-4	0.0	0.0	12.619197394746344	0.0
20	2.2709672730906276E-4	0.0	0.0	13.09519213518614	0.0
21	3.406450909635941E-4	0.0	0.0	13.642835893091945	0.0
22	3.406450909635941E-4	0.0	0.0	14.281999632103302	0.0
23	3.406450909635941E-4	0.0	0.0	14.884146604563282	0.0
24	3.406450909635941E-4	0.0	0.0	15.317901353723592	0.0
25	3.406450909635941E-4	0.0	0.0	15.702830306512453	0.0
26	3.406450909635941E-4	0.0	0.0	16.06266507093366	0.0
27	3.406450909635941E-4	0.0	0.0	16.472461115362865	0.0
28	3.406450909635941E-4	0.0	0.0	16.907237799796068	0.0
29	3.406450909635941E-4	0.0	0.0	17.33031900277285	0.0
30	3.406450909635941E-4	0.0	0.0	17.893405338135672	0.0
31	3.406450909635941E-4	0.0	0.0	18.317508476385346	0.0
32	3.406450909635941E-4	0.0	0.0	18.718674845176807	0.0
33	3.406450909635941E-4	0.0	0.0	19.125177987060027	0.0
34	3.406450909635941E-4	0.0	0.0	19.522824356578198	0.0
35	3.406450909635941E-4	0.0	0.0	19.975087489014197	0.0
36	3.406450909635941E-4	0.0	0.0	20.409523528356434	0.0
37	3.406450909635941E-4	0.0	0.0	20.862581499338013	0.0
38	3.406450909635941E-4	0.0	0.0	21.30031044122623	0.0
39	3.406450909635941E-4	0.0	0.0	21.73531422238674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	45	3.8380676E-10	45.0	1
CGAGTCC	20	7.032388E-4	45.0	13
CGTTGCG	40	6.8121153E-9	45.0	1
CCGGACC	20	7.032388E-4	45.0	5
TAACGCG	75	0.0	45.0	1
GTTACGG	110	0.0	45.0	2
ACGATAG	20	7.032388E-4	45.0	1
CCGTAAC	25	3.8900605E-5	45.0	23
CGCTCAG	45	3.8380676E-10	45.0	1
TATCTCG	45	3.8380676E-10	45.0	1
TCGTCCA	20	7.032388E-4	45.0	28
CTTGACG	20	7.032388E-4	45.0	1
TTGCGAA	25	3.8900605E-5	45.0	24
ATAAGCG	25	3.8900605E-5	45.0	1
AGCGAAT	20	7.032388E-4	45.0	35
TCGTAAG	25	3.8900605E-5	45.0	1
CATAACG	55	1.8189894E-12	45.0	1
ATGCGAT	25	3.8900605E-5	45.0	12
ATACGAG	20	7.032388E-4	45.0	1
TATCGTC	20	7.032388E-4	45.0	15
>>END_MODULE