FastQCFastQC Report
Sat 18 Jun 2016
SRR3554085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893898
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT38800.4340539972122099No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCC12460.13938950529031277No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT12180.13625715685682258No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT11420.127755068251635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.032443E-445.0000041
TCCGCTC207.032443E-445.00000423
GATCGCG207.032443E-445.0000049
CCGATAG207.032443E-445.0000041
CGGTTGC207.032443E-445.00000431
GCCGGTT207.032443E-445.00000428
TTCCGCG207.032443E-445.0000041
CGACACT207.032443E-445.00000429
TAACGCG650.045.0000041
TCGGATC207.032443E-445.00000424
ACGATAG207.032443E-445.0000041
CGCTCCG207.032443E-445.0000041
CCGACCG207.032443E-445.0000041
ACCGTAT207.032443E-445.00000426
CGACTCG406.8121153E-945.0000041
TGTACGA207.032443E-445.00000438
TTACCCG207.032443E-445.0000041
CCACGTA207.032443E-445.00000419
TATCGCG406.8121153E-945.0000041
TAGTCCG207.032443E-445.0000041