##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893898
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95677023553023	33.0	31.0	34.0	30.0	34.0
2	32.158606462929775	34.0	31.0	34.0	30.0	34.0
3	32.30081508181023	34.0	31.0	34.0	30.0	34.0
4	35.87062617882577	37.0	35.0	37.0	35.0	37.0
5	35.70477056666421	37.0	35.0	37.0	33.0	37.0
6	35.712520891645354	37.0	35.0	37.0	33.0	37.0
7	35.86600708358225	37.0	35.0	37.0	35.0	37.0
8	36.032115521010226	37.0	35.0	37.0	35.0	37.0
9	37.647861389107035	39.0	37.0	39.0	35.0	39.0
10	37.173393384927586	39.0	37.0	39.0	34.0	39.0
11	37.12633879928135	39.0	37.0	39.0	34.0	39.0
12	37.13352977632795	39.0	37.0	39.0	33.0	39.0
13	37.121325923091895	39.0	37.0	39.0	33.0	39.0
14	38.35042588751737	40.0	38.0	41.0	34.0	41.0
15	38.32989781831932	40.0	38.0	41.0	34.0	41.0
16	38.2921843431801	40.0	38.0	41.0	33.0	41.0
17	38.26448431476522	40.0	38.0	41.0	33.0	41.0
18	38.20887282441621	40.0	37.0	41.0	33.0	41.0
19	38.239755542578685	40.0	38.0	41.0	34.0	41.0
20	38.164309574470465	40.0	37.0	41.0	33.0	41.0
21	38.17187867072082	40.0	37.0	41.0	34.0	41.0
22	38.167481077259374	40.0	37.0	41.0	34.0	41.0
23	38.08947777039438	40.0	37.0	41.0	33.0	41.0
24	38.01856475794777	40.0	37.0	41.0	33.0	41.0
25	37.99006933676997	40.0	37.0	41.0	33.0	41.0
26	37.923673618242795	40.0	37.0	41.0	33.0	41.0
27	37.863981125363296	40.0	37.0	41.0	33.0	41.0
28	37.83051981322254	40.0	37.0	41.0	33.0	41.0
29	37.763019941872564	40.0	37.0	41.0	33.0	41.0
30	37.749860722364296	40.0	37.0	41.0	33.0	41.0
31	37.70958431498896	40.0	37.0	41.0	33.0	41.0
32	37.696548152026295	40.0	37.0	41.0	33.0	41.0
33	37.64780657300945	40.0	37.0	41.0	33.0	41.0
34	37.5980514555352	40.0	37.0	41.0	33.0	41.0
35	37.527205564840735	40.0	37.0	41.0	32.0	41.0
36	37.520158899561245	40.0	37.0	41.0	32.0	41.0
37	37.45879395635744	40.0	37.0	41.0	32.0	41.0
38	37.433310064459256	40.0	36.0	41.0	32.0	41.0
39	37.39492649049444	40.0	36.0	41.0	32.0	41.0
40	37.30719947913521	39.0	36.0	41.0	32.0	41.0
41	37.255863644397905	39.0	36.0	41.0	32.0	41.0
42	37.17514190657099	39.0	36.0	41.0	31.0	41.0
43	37.098137595117116	39.0	36.0	41.0	31.0	41.0
44	37.056097004356204	39.0	36.0	41.0	31.0	41.0
45	36.919046692128184	39.0	35.0	41.0	31.0	41.0
46	36.793766179139006	39.0	35.0	41.0	31.0	41.0
47	36.68264947454855	39.0	35.0	40.0	31.0	41.0
48	36.65369762545615	39.0	35.0	40.0	31.0	41.0
49	36.516890070231725	39.0	35.0	40.0	30.0	41.0
50	36.37000082783494	39.0	35.0	40.0	30.0	41.0
51	35.22616226907321	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	9.0
12	10.0
13	18.0
14	11.0
15	24.0
16	51.0
17	91.0
18	164.0
19	285.0
20	526.0
21	941.0
22	1349.0
23	1957.0
24	2468.0
25	3371.0
26	4058.0
27	5622.0
28	7558.0
29	10148.0
30	13434.0
31	17931.0
32	23491.0
33	31240.0
34	45750.0
35	59212.0
36	74596.0
37	112833.0
38	195009.0
39	281664.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.812996561128898	33.285453150135695	22.323128589615372	25.578421699120035
2	21.89746481142144	30.980939659782216	21.50446695260533	25.617128576191018
3	20.00205840039915	31.240588971001166	22.136753857822704	26.620598770776976
4	20.46978514327138	32.32270348518511	19.76545422408373	27.442057147459774
5	19.2826250869786	33.67162696415027	17.838276850378904	29.20747109849222
6	20.479405927745674	36.95287381781814	18.438904662500644	24.128815591935545
7	88.37283448447138	3.6591423182510754	4.225873645538977	3.7421495517385654
8	90.19272892432917	2.85994598936344	3.1765369203197684	3.770788165987618
9	81.73941545903448	8.457005161662739	6.281029826669263	3.522549552633522
10	30.756976746787668	45.70622151520644	11.275111925521704	12.261689812484198
11	20.79264077109469	26.72542057371199	34.67509715873623	17.80684149645709
12	24.848696383703732	24.12143219919946	32.43513242003003	18.594738997066777
13	24.63200499385836	25.13083148189167	29.292715723717915	20.94444780053205
14	19.52202600296678	27.92074710984922	30.090010269628074	22.46721661755592
15	18.882467574600234	30.70887282441621	30.05298143636075	20.35567816462281
16	22.269543057485304	28.995366361710172	29.116185515573367	19.618905065231157
17	21.471241685292952	29.141132433454374	28.780576754842276	20.607049126410395
18	21.0021725073778	28.427628208140078	28.448435951305406	22.121763333176716
19	21.54418065595851	30.69063808174982	28.94547252594815	18.81970873634352
20	23.232292722435893	29.356033909909186	28.114393364791063	19.29728000286386
21	22.74756180235329	29.453136711347376	28.09448057832102	19.704820907978316
22	22.051173623836277	26.678659086383455	29.103432382665584	22.166734907114684
23	21.16225788624653	29.019306453308992	28.206685773992113	21.61174988645237
24	19.420895896399813	29.55751103593475	28.8715267290004	22.150066338665038
25	21.015708727393953	30.735945264448517	26.386567594960496	21.86177841319703
26	21.783693441533597	31.08106294006699	26.483334787637965	20.65190883076145
27	19.57035366451206	29.74847242079074	28.133075585805088	22.54809832889211
28	20.071305674696667	29.787850515383184	28.540280882158815	21.60056292776133
29	22.057214581529436	27.864700446807127	27.979590512564073	22.09849445909936
30	20.593289167220423	28.206909513165932	28.32761679744221	22.87218452217143
31	21.261262470662203	29.757421987743566	26.86123025222117	22.12008528937306
32	22.229717484545215	27.58256534861919	27.585138349118132	22.602578817717458
33	21.240678466670694	28.442506863199156	28.266088524641514	22.050726145488635
34	20.874081830365434	27.21820610405214	28.93864848114662	22.96906358443581
35	21.66097250469293	27.515107987712245	29.064054288073137	21.75986521952169
36	20.062915455678386	28.913142215331057	29.513546288278974	21.51039604071158
37	21.56554774705839	27.172787051766534	28.79109249601185	22.470572705163228
38	21.10061774385892	26.7071858310456	31.50426558734889	20.68793083774659
39	20.785145508771695	26.447089041479003	30.44161638128735	22.32614906846195
40	22.66220530754068	26.654495255610822	28.679111039514577	22.004188397333923
41	20.641840567939518	27.065951596267134	29.870298400936125	22.42190943485722
42	23.241466028562545	27.84624196496692	26.566118281951635	22.346173724518906
43	21.422802154160767	27.340032084197524	28.576862237078505	22.660303524563204
44	21.733016518663202	26.153766984600036	27.77251990719299	24.34069658954377
45	21.58825727320119	26.743431577204557	28.64711633765821	23.021194811936038
46	19.614877760102384	27.093024036299447	30.448664165262702	22.84343403833547
47	20.54954815873847	27.181848488306272	28.85306824716019	23.41553510579507
48	21.460166596188827	25.273465205202385	30.59789819420113	22.668470004407663
49	21.427948155158642	25.613213140649155	29.08497390082537	23.873864803366825
50	20.19212482855986	25.220103412246143	29.133860910305202	25.4539108488888
51	19.85774663328478	24.282636273937293	31.020429623961572	24.839187468816355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	758.0
1	639.0
2	520.0
3	384.5
4	249.0
5	249.5
6	250.0
7	289.5
8	329.0
9	422.0
10	515.0
11	536.0
12	557.0
13	792.5
14	1028.0
15	1051.5
16	1075.0
17	1344.0
18	1613.0
19	1717.5
20	1822.0
21	2324.0
22	2826.0
23	3574.5
24	4323.0
25	5987.5
26	9565.5
27	11479.0
28	13493.0
29	15507.0
30	18454.5
31	21402.0
32	24663.5
33	27925.0
34	32603.0
35	37281.0
36	39232.5
37	41184.0
38	43825.5
39	46467.0
40	49015.0
41	51563.0
42	53849.5
43	56136.0
44	63108.0
45	70080.0
46	74618.0
47	79156.0
48	84974.0
49	90792.0
50	83915.5
51	77039.0
52	69103.5
53	61168.0
54	54609.0
55	48050.0
56	41595.0
57	35140.0
58	31749.5
59	28359.0
60	26407.0
61	24455.0
62	22290.5
63	20126.0
64	16385.5
65	12645.0
66	9428.5
67	6212.0
68	4753.5
69	3295.0
70	2764.5
71	2234.0
72	1637.0
73	1040.0
74	875.0
75	550.5
76	391.0
77	361.5
78	332.0
79	189.5
80	47.0
81	36.5
82	26.0
83	19.0
84	12.0
85	39.5
86	67.0
87	41.5
88	16.0
89	19.0
90	22.0
91	15.5
92	9.0
93	9.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	893898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.86752264912311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0892554224581	18.90637970047979
2	11.147594832869835	5.767213236450164
3	4.032248916459942	3.129128705202889
4	1.9685575876791366	2.036868319415728
5	1.1795684457501026	1.525625674331586
6	0.7562902213524778	1.173801265808733
7	0.5559641917143091	1.006699142488992
8	0.451472256652327	0.9342775059523831
9	0.3566126972660975	0.8302218321146094
>10	4.772709213999604	29.9042777549218
>50	1.4549816407957175	26.02885733982514
>100	0.2312668756245443	7.641602565563301
>500	0.001738848688906348	0.2732517251503741
>1k	0.001738848688906348	0.8417952322945269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT	3880	0.4340539972122099	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCC	1246	0.13938950529031277	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT	1218	0.13625715685682258	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT	1142	0.127755068251635	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48819887727682576	0.0
2	0.0	0.0	0.0	0.6887810466071073	0.0
3	1.1186958691036338E-4	0.0	0.0	0.9204629610984698	0.0
4	1.1186958691036338E-4	0.0	0.0	2.0224902617524596	0.0
5	1.1186958691036338E-4	0.0	0.0	2.182128162273548	0.0
6	1.1186958691036338E-4	0.0	0.0	3.2031618820044345	0.0
7	1.1186958691036338E-4	0.0	0.0	3.942507981895026	0.0
8	1.1186958691036338E-4	0.0	0.0	4.606453980208033	0.0
9	1.1186958691036338E-4	0.0	0.0	5.6056731304913985	0.0
10	1.1186958691036338E-4	0.0	0.0	6.939046736876019	0.0
11	1.1186958691036338E-4	0.0	0.0	8.37030623180721	0.0
12	1.1186958691036338E-4	0.0	0.0	9.034475969294036	0.0
13	1.1186958691036338E-4	0.0	0.0	9.308780196398247	0.0
14	1.1186958691036338E-4	0.0	0.0	9.634208824720494	0.0
15	1.1186958691036338E-4	0.0	0.0	9.898556658589683	0.0
16	1.1186958691036338E-4	0.0	0.0	10.437096849976172	0.0
17	1.1186958691036338E-4	0.0	0.0	11.157984468026553	0.0
18	1.1186958691036338E-4	0.0	0.0	11.981120888513008	0.0
19	1.1186958691036338E-4	0.0	0.0	12.40063183942687	0.0
20	1.1186958691036338E-4	0.0	0.0	12.871602800319499	0.0
21	1.1186958691036338E-4	0.0	0.0	13.406786904098677	0.0
22	1.1186958691036338E-4	0.0	0.0	14.008980890437163	0.0
23	1.1186958691036338E-4	0.0	0.0	14.605693267017042	0.0
24	1.1186958691036338E-4	0.0	0.0	15.026211044213099	0.0
25	1.1186958691036338E-4	0.0	0.0	15.397953681516235	0.0
26	1.1186958691036338E-4	0.0	0.0	15.764326578647676	0.0
27	1.1186958691036338E-4	0.0	0.0	16.160680525071093	0.0
28	1.1186958691036338E-4	0.0	0.0	16.588917303763964	0.0
29	1.1186958691036338E-4	0.0	0.0	17.030466563299168	0.0
30	1.1186958691036338E-4	0.0	0.0	17.56374888410087	0.0
31	1.1186958691036338E-4	0.0	0.0	17.993439967423576	0.0
32	1.1186958691036338E-4	0.0	0.0	18.396841697822346	0.0
33	1.1186958691036338E-4	0.0	0.0	18.80091464574258	0.0
34	1.1186958691036338E-4	0.0	0.0	19.19234633034194	0.0
35	1.1186958691036338E-4	0.0	0.0	19.649557332044594	0.0
36	1.1186958691036338E-4	0.0	0.0	20.072088761805038	0.0
37	1.1186958691036338E-4	0.0	0.0	20.53075406813753	0.0
38	1.1186958691036338E-4	0.0	0.0	20.964136847828275	0.0
39	1.1186958691036338E-4	0.0	0.0	21.394499148672445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.032443E-4	45.000004	1
TCCGCTC	20	7.032443E-4	45.000004	23
GATCGCG	20	7.032443E-4	45.000004	9
CCGATAG	20	7.032443E-4	45.000004	1
CGGTTGC	20	7.032443E-4	45.000004	31
GCCGGTT	20	7.032443E-4	45.000004	28
TTCCGCG	20	7.032443E-4	45.000004	1
CGACACT	20	7.032443E-4	45.000004	29
TAACGCG	65	0.0	45.000004	1
TCGGATC	20	7.032443E-4	45.000004	24
ACGATAG	20	7.032443E-4	45.000004	1
CGCTCCG	20	7.032443E-4	45.000004	1
CCGACCG	20	7.032443E-4	45.000004	1
ACCGTAT	20	7.032443E-4	45.000004	26
CGACTCG	40	6.8121153E-9	45.000004	1
TGTACGA	20	7.032443E-4	45.000004	38
TTACCCG	20	7.032443E-4	45.000004	1
CCACGTA	20	7.032443E-4	45.000004	19
TATCGCG	40	6.8121153E-9	45.000004	1
TAGTCCG	20	7.032443E-4	45.000004	1
>>END_MODULE