FastQCFastQC Report
Sat 18 Jun 2016
SRR3554084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266759
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT26730.21101093420295416TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG302.1656779E-645.0000041
CGTATCG207.03356E-445.01
TCGTAAG207.03356E-445.01
ATACGTC207.03356E-445.020
CCAACCG1400.040.178571
CGTTATG451.9284926E-840.01
TTACCGG2200.039.8863642
TATACGG850.039.7058832
TATAACG850.039.7058831
ATAGCGG2100.039.6428532
CCACGCG1250.039.6000021
ATTACCG1200.039.3750041
GTCGACG403.45859E-739.37535
TACTACG750.039.01
ATAACGG2150.038.7209322
TAACACG1400.038.5714261
TATAGCG356.2487943E-638.5714261
CGTAACG700.038.5714261
TATACGT356.2487943E-638.57142624
CGCATAG1000.038.2500041
Produced by FastQC (version 0.11.5)