##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716493
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97186015774055	33.0	31.0	34.0	30.0	34.0
2	32.17377141158392	34.0	31.0	34.0	30.0	34.0
3	32.310339389219436	34.0	31.0	34.0	30.0	34.0
4	35.923097643661556	37.0	35.0	37.0	35.0	37.0
5	35.73474130242724	37.0	35.0	37.0	35.0	37.0
6	35.736933926779464	37.0	35.0	37.0	33.0	37.0
7	35.843930087244395	37.0	35.0	37.0	35.0	37.0
8	36.00846763331952	37.0	35.0	37.0	35.0	37.0
9	37.650165458699526	39.0	37.0	39.0	35.0	39.0
10	37.18311972343066	39.0	37.0	39.0	34.0	39.0
11	37.130717257530776	39.0	37.0	39.0	34.0	39.0
12	37.07096789501084	39.0	37.0	39.0	33.0	39.0
13	37.0531435757223	39.0	37.0	39.0	33.0	39.0
14	38.253654955456646	40.0	38.0	41.0	33.0	41.0
15	38.24969678698885	40.0	38.0	41.0	33.0	41.0
16	38.198395518169754	40.0	37.0	41.0	33.0	41.0
17	38.19944367914271	40.0	37.0	41.0	33.0	41.0
18	38.15223037768687	40.0	37.0	41.0	33.0	41.0
19	38.165799247166404	40.0	37.0	41.0	34.0	41.0
20	38.0855374721037	40.0	37.0	41.0	33.0	41.0
21	38.0931593190722	40.0	37.0	41.0	33.0	41.0
22	38.07104047073733	40.0	37.0	41.0	33.0	41.0
23	37.97961878203974	40.0	37.0	41.0	33.0	41.0
24	37.881090254894325	40.0	37.0	41.0	33.0	41.0
25	37.910113567055085	40.0	37.0	41.0	33.0	41.0
26	37.857113747098715	40.0	37.0	41.0	33.0	41.0
27	37.82353630810071	40.0	37.0	41.0	33.0	41.0
28	37.782705483514846	40.0	37.0	41.0	33.0	41.0
29	37.719226845202954	40.0	37.0	41.0	33.0	41.0
30	37.68262774374627	40.0	37.0	41.0	33.0	41.0
31	37.539696828859455	40.0	36.0	41.0	32.0	41.0
32	37.534150368531165	40.0	36.0	41.0	33.0	41.0
33	37.431811615745026	39.0	36.0	41.0	32.0	41.0
34	37.3262962792379	39.0	36.0	41.0	32.0	41.0
35	37.30418161796417	39.0	36.0	41.0	32.0	41.0
36	37.20094543840624	39.0	36.0	41.0	31.0	41.0
37	37.09246147554826	39.0	35.0	41.0	31.0	41.0
38	37.111854547078615	39.0	35.0	41.0	31.0	41.0
39	36.999745984957286	39.0	35.0	41.0	31.0	41.0
40	36.89303733602422	39.0	35.0	41.0	31.0	41.0
41	36.83473809234703	39.0	35.0	41.0	31.0	41.0
42	36.816562059922425	39.0	35.0	40.0	31.0	41.0
43	36.800532594177476	39.0	35.0	40.0	31.0	41.0
44	36.70438790050984	39.0	35.0	40.0	31.0	41.0
45	36.562752183203465	39.0	35.0	40.0	30.0	41.0
46	36.400650111026906	39.0	35.0	40.0	30.0	41.0
47	36.28587159958297	38.0	35.0	40.0	30.0	41.0
48	36.21464410678122	38.0	35.0	40.0	30.0	41.0
49	36.05369487210622	38.0	35.0	40.0	29.0	41.0
50	35.91348275558868	38.0	35.0	40.0	29.0	41.0
51	34.56934959587881	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	5.0
11	3.0
12	7.0
13	8.0
14	16.0
15	21.0
16	36.0
17	75.0
18	150.0
19	236.0
20	464.0
21	734.0
22	1120.0
23	1555.0
24	2042.0
25	2683.0
26	3640.0
27	4830.0
28	6379.0
29	8673.0
30	11458.0
31	15182.0
32	20062.0
33	27319.0
34	42008.0
35	51141.0
36	64434.0
37	96231.0
38	155863.0
39	200067.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.606342281083	32.84093494283964	20.83774719362227	25.714975582455097
2	21.298882194243347	31.971840618121877	21.710749442074103	25.01852774556067
3	20.398524479652977	31.651809578041934	22.134759167221453	25.814906775083635
4	20.580382501992343	31.570859729264626	19.784282609878954	28.064475158864077
5	18.89718392224348	33.409537846147835	18.34463141998596	29.348646811622725
6	22.150530430862545	34.094401480544825	20.06690923707559	23.68815885151704
7	86.40531031007978	4.028092388899822	5.28839779313964	4.278199507880747
8	88.17336666234003	3.5710048807176062	3.6833576880723187	4.5722707688700375
9	79.80426884840466	8.307408446418876	7.627708854099064	4.260613851077401
10	33.63522044179078	39.13590223491367	12.711917632133183	14.51695969116237
11	23.563105292026577	25.771221770484846	32.08461213159096	18.581060805897614
12	26.285392878925546	22.20663705018751	31.634084352533797	19.873885718353147
13	24.77874871073409	24.253412105910314	28.949061609813352	22.018777573542238
14	19.885749058260163	25.94540351406085	31.420404665502666	22.748442762176325
15	19.54506184987153	29.030151027295453	30.45724103375748	20.96754608907554
16	23.01739165630369	27.138576371297418	27.858192613186734	21.98583935921216
17	21.92401042299087	27.71108719833969	29.3924713849263	20.972430993743135
18	22.4511614209769	27.008079632320204	28.466014322540484	22.074744624162413
19	22.94411808628975	29.178512560485586	28.035584436972865	19.841784916251797
20	23.754453986291562	28.456802788024447	27.41966774274138	20.369075482942613
21	23.82730885019114	27.39705761256565	27.385473410068208	21.390160127175005
22	22.210824111331164	25.522789475961382	28.96678683532149	23.299599577385962
23	22.907969791749537	26.66711328652199	27.241159369316936	23.18375755241154
24	20.4780786413824	28.500069086508873	28.205858256814793	22.81599401529394
25	21.906285197482738	27.66349427000683	26.568577780941332	23.8616427515691
26	22.294146628089877	28.67620479195191	26.017560534436484	23.01208804552173
27	20.277937118715744	27.043250945926893	28.514025956987716	24.164785978369643
28	20.419878491485612	27.03724949162099	30.11250633292998	22.430365683963416
29	22.355556858196802	26.14973209787116	26.912335500835315	24.58237554309672
30	24.37260377979966	26.77904738776234	26.690421260221665	22.157927572216337
31	22.472794570219108	27.603340158242993	26.939132692154704	22.98473257938319
32	26.02300371392323	25.78364338521102	24.589912253155298	23.603440647710443
33	24.226894052000507	25.701018711976253	27.838513425811556	22.233573810211684
34	21.353453557815637	25.080496250486746	30.39764519681281	23.168404994884806
35	23.23804977857425	26.43668535491624	28.231120192381503	22.09414467412801
36	23.090665226317633	26.876605912409474	29.011588389558586	21.021140471714308
37	23.417535132932212	27.557142916958018	27.25232486570001	21.772997084409756
38	25.027320573962342	25.375823629819134	29.30552008184309	20.291335714375435
39	23.96422574958862	25.569265854655942	27.821067337713	22.645441058042437
40	23.678109904772274	26.891958469936206	27.45344336930019	21.976488255991335
41	20.85379759467294	25.321112697542052	31.11363265237762	22.71145705540738
42	22.752769392024767	26.70521554292924	27.057626522520106	23.484388542525885
43	20.994203711690133	25.9495905752045	28.60572259603374	24.450483117071624
44	23.8084670750447	23.34607595608052	27.497965786127708	25.347491182747074
45	24.654811700881936	24.313426648969354	27.548768794670707	23.482992855478003
46	20.871662388885863	25.739958380612233	29.529388284323783	23.85899094617812
47	22.4257599167054	25.71986048712269	28.179340202904985	23.67503939326693
48	22.62352877139065	23.74370719602285	31.168762290769063	22.464001741817434
49	23.168404994884806	24.48635227420226	28.940966624935623	23.40427610597731
50	21.922056461123834	23.17817480422	28.603349928052335	26.296418806603832
51	21.685766643916967	22.549557357852766	30.571408234274443	25.193267763955824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	918.0
1	722.5
2	527.0
3	374.0
4	221.0
5	229.5
6	238.0
7	255.0
8	272.0
9	342.0
10	412.0
11	468.5
12	525.0
13	624.0
14	723.0
15	782.0
16	841.0
17	908.0
18	975.0
19	1028.0
20	1081.0
21	1345.5
22	1610.0
23	2040.0
24	2470.0
25	3274.0
26	5481.5
27	6885.0
28	7685.0
29	8485.0
30	10740.5
31	12996.0
32	15435.5
33	17875.0
34	20426.5
35	22978.0
36	24596.5
37	26215.0
38	28307.0
39	30399.0
40	33081.5
41	35764.0
42	38306.0
43	40848.0
44	47568.5
45	54289.0
46	59248.5
47	64208.0
48	69161.5
49	74115.0
50	68765.0
51	63415.0
52	58759.5
53	54104.0
54	47187.0
55	40270.0
56	38057.0
57	35844.0
58	32822.0
59	29800.0
60	28133.0
61	26466.0
62	22558.0
63	18650.0
64	16491.0
65	14332.0
66	11519.5
67	8707.0
68	6999.0
69	5291.0
70	4472.0
71	3653.0
72	3033.0
73	2413.0
74	2098.0
75	1229.0
76	675.0
77	609.5
78	544.0
79	421.0
80	298.0
81	221.0
82	144.0
83	97.5
84	51.0
85	37.0
86	23.0
87	20.5
88	18.0
89	13.5
90	9.0
91	12.5
92	16.0
93	9.5
94	3.0
95	6.5
96	10.0
97	17.5
98	25.0
99	13.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	716493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.947616454673124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03418273533015	20.411313271675166
2	11.118820031610202	6.214890353439568
3	4.044357036803551	3.3909041781103184
4	2.0151497633846387	2.252745307431967
5	1.1852707021647242	1.6562745489530406
6	0.7705026235237942	1.292022707973744
7	0.5609635206658009	1.0974315324441632
8	0.49230216357104223	1.100693763783139
9	0.3625453845272723	0.9119051418762172
>10	5.169580693341954	34.901954733866255
>50	1.1532263433965173	21.573068419808124
>100	0.08859421127645462	3.686297961316306
>500	0.002502661335493068	0.4702995501648991
>1k	0.0020021290683944547	1.0401985291571059
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	3524	0.4918401156745425	TruSeq Adapter, Index 15 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	1449	0.20223505323848245	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	1264	0.17641484285261685	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGT	1199	0.16734287704136677	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	777	0.10844488362063551	TruSeq Adapter, Index 21 (95% over 22bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	751	0.10481609729613547	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	726	0.10132687967642391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5598100749065239	0.0
2	0.0	0.0	0.0	0.819268297108276	0.0
3	0.0	0.0	0.0	1.129669096557817	0.0
4	0.0	0.0	0.0	2.5884411990068292	0.0
5	0.0	0.0	0.0	2.8057496723624653	0.0
6	0.0	0.0	0.0	4.047632007570207	0.0
7	0.0	0.0	0.0	4.915609782649656	0.0
8	0.0	0.0	0.0	5.600054710932277	0.0
9	0.0	0.0	0.0	6.777177167118172	0.0
10	0.0	0.0	0.0	8.322761003945608	0.0
11	0.0	0.0	0.0	10.003307778303487	0.0
12	0.0	0.0	0.0	10.743301051091915	0.0
13	1.395687047884627E-4	0.0	0.0	11.056632793342015	0.0
14	1.395687047884627E-4	0.0	0.0	11.425373311393132	0.0
15	1.395687047884627E-4	0.0	0.0	11.739961171986328	0.0
16	1.395687047884627E-4	0.0	0.0	12.341502289624602	0.0
17	1.395687047884627E-4	0.0	0.0	13.15965403709457	0.0
18	1.395687047884627E-4	0.0	0.0	14.025538281602193	0.0
19	1.395687047884627E-4	0.0	0.0	14.52966044329812	0.0
20	1.395687047884627E-4	0.0	0.0	15.085562594470566	0.0
21	1.395687047884627E-4	0.0	0.0	15.693105166414744	0.0
22	1.395687047884627E-4	0.0	0.0	16.367640716657384	0.0
23	1.395687047884627E-4	0.0	0.0	17.01817044967641	0.0
24	1.395687047884627E-4	0.0	0.0	17.520757355619665	0.0
25	1.395687047884627E-4	0.0	0.0	17.954676458806993	0.0
26	1.395687047884627E-4	0.0	0.0	18.35635519118819	0.0
27	1.395687047884627E-4	0.0	0.0	18.812605287141675	0.0
28	1.395687047884627E-4	0.0	0.0	19.268994951799947	0.0
29	1.395687047884627E-4	0.0	0.0	19.72719900962047	0.0
30	1.395687047884627E-4	0.0	0.0	20.27919323705884	0.0
31	1.395687047884627E-4	0.0	0.0	20.736280745241057	0.0
32	1.395687047884627E-4	0.0	0.0	21.175922165324714	0.0
33	1.395687047884627E-4	0.0	0.0	21.623519001581315	0.0
34	1.395687047884627E-4	0.0	0.0	22.060229478864414	0.0
35	1.395687047884627E-4	0.0	0.0	22.553325712882053	0.0
36	1.395687047884627E-4	0.0	0.0	23.005947022511037	0.0
37	1.395687047884627E-4	0.0	0.0	23.46066186271185	0.0
38	1.395687047884627E-4	0.0	0.0	23.903932069120007	0.0
39	1.395687047884627E-4	0.0	0.0	24.37734911576247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAA	30	2.1643518E-6	45.000004	39
TTCACCG	30	2.1643518E-6	45.000004	1
CTAGGCG	30	2.1643518E-6	45.000004	1
AACGTTG	20	7.0315064E-4	45.0	17
GCGAATG	35	1.211065E-7	45.0	1
CTATCGT	20	7.0315064E-4	45.0	23
GCGCGAC	20	7.0315064E-4	45.0	9
GTTTACG	20	7.0315064E-4	45.0	42
CGCATCA	20	7.0315064E-4	45.0	20
GGCCGTA	20	7.0315064E-4	45.0	30
TAGCGAG	40	6.8084773E-9	45.0	1
ATTCGGC	20	7.0315064E-4	45.0	10
CCTAACG	20	7.0315064E-4	45.0	1
TCTAGCG	35	1.211065E-7	45.0	1
AACGGTC	35	1.211065E-7	45.0	4
TACGCGG	70	0.0	45.0	2
CGTAGTT	20	7.0315064E-4	45.0	15
CAAATCG	20	7.0315064E-4	45.0	1
TTGCGAT	20	7.0315064E-4	45.0	28
CGTACCG	20	7.0315064E-4	45.0	1
>>END_MODULE