SRR3554080_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785326
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	5001	0.6368056068435274	TruSeq Adapter, Index 1 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	1840	0.23429760379765854	RNA PCR Primer, Index 40 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	1380	0.17572320284824391	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGT	1164	0.14821870153286662	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1063	0.13535780045484297	No Hit
AAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	985	0.12542561942429004	RNA PCR Primer, Index 40 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	878	0.11180070442083924	RNA PCR Primer, Index 1 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.211265E-7	45.000004	1
TTTACCG	30	2.1646192E-6	45.000004	1
TAGCGGA	35	1.211265E-7	45.000004	3
TAGCGCG	35	1.211265E-7	45.000004	1
ATTAACG	35	1.211265E-7	45.000004	1
TCTACCG	35	1.211265E-7	45.000004	1
CTACGCG	35	1.211265E-7	45.000004	1
CACTACG	60	0.0	45.000004	1
TCTAACG	35	1.211265E-7	45.000004	1
TCTCGCG	35	1.211265E-7	45.000004	1
TTACGCG	35	1.211265E-7	45.000004	1
CGCGTAG	30	2.1646192E-6	45.000004	1
CTTACCG	30	2.1646192E-6	45.000004	1
TAATCGG	30	2.1646192E-6	45.000004	2
GCATAAC	25	3.889673E-5	45.0	36
TGATCCG	25	3.889673E-5	45.0	3
TTTAGCG	20	7.0319214E-4	45.0	1
AGCGTAT	20	7.0319214E-4	45.0	37
TACCCGA	20	7.0319214E-4	45.0	31
CGAACAC	20	7.0319214E-4	45.0	16