SRR3554079_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794550
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	5146	0.6476621987288402	TruSeq Adapter, Index 1 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	1809	0.227676043043232	RNA PCR Primer, Index 40 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	1428	0.17972437228619972	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGT	1208	0.15203574350261154	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1047	0.13177270152916745	No Hit
AAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	953	0.11994210559436158	RNA PCR Primer, Index 40 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	852	0.10723050783462337	RNA PCR Primer, Index 1 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACCG	30	2.164652E-6	45.000004	1
CGAAACG	35	1.2113014E-7	45.000004	1
CCGGCCG	30	2.164652E-6	45.000004	1
CGCGACG	30	2.164652E-6	45.000004	1
CGACAAG	35	1.2113014E-7	45.000004	1
TGACCTA	30	2.164652E-6	45.000004	28
CGGATCA	35	1.2113014E-7	45.000004	6
CTACGCG	30	2.164652E-6	45.000004	1
CACTACG	65	0.0	45.000004	1
CTTGCGT	30	2.164652E-6	45.000004	18
CGCTACG	60	0.0	45.000004	1
AAACCCG	35	1.2113014E-7	45.000004	1
ACGTGAC	30	2.164652E-6	45.000004	37
GAACGTA	35	1.2113014E-7	45.000004	9
CGTACTA	35	1.2113014E-7	45.000004	12
CGAGCAA	30	2.164652E-6	45.000004	1
ACGTACG	30	2.164652E-6	45.000004	1
ACCAACG	30	2.164652E-6	45.000004	1
CGCGTTG	35	1.2113014E-7	45.000004	1
CGCGTAG	35	1.2113014E-7	45.000004	1