##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794550
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86152790887924	33.0	31.0	34.0	30.0	34.0
2	32.05666603737965	33.0	31.0	34.0	30.0	34.0
3	32.215839154238246	34.0	31.0	34.0	30.0	34.0
4	35.80948209678434	37.0	35.0	37.0	35.0	37.0
5	35.648374551633	37.0	35.0	37.0	33.0	37.0
6	35.67053300610409	37.0	35.0	37.0	33.0	37.0
7	35.84951733685734	37.0	35.0	37.0	35.0	37.0
8	36.03560631804166	37.0	35.0	37.0	35.0	37.0
9	37.67004971367441	39.0	37.0	39.0	35.0	39.0
10	37.122909823170346	39.0	37.0	39.0	33.0	39.0
11	37.11722861997357	39.0	37.0	39.0	34.0	39.0
12	37.140622994147634	39.0	37.0	39.0	33.0	39.0
13	37.08658485935435	39.0	37.0	39.0	33.0	39.0
14	38.291830595934805	40.0	38.0	41.0	34.0	41.0
15	38.27797117865458	40.0	38.0	41.0	33.0	41.0
16	38.251862060285696	40.0	38.0	41.0	33.0	41.0
17	38.22882763828582	40.0	38.0	41.0	33.0	41.0
18	38.18906425020452	40.0	37.0	41.0	33.0	41.0
19	38.19932414574287	40.0	37.0	41.0	34.0	41.0
20	38.13865206720785	40.0	37.0	41.0	33.0	41.0
21	38.13069787930275	40.0	37.0	41.0	33.0	41.0
22	38.1207702473098	40.0	37.0	41.0	33.0	41.0
23	38.049002580076774	40.0	37.0	41.0	33.0	41.0
24	37.93972185513813	40.0	37.0	41.0	33.0	41.0
25	37.96705934176578	40.0	37.0	41.0	33.0	41.0
26	37.893717198414194	40.0	37.0	41.0	33.0	41.0
27	37.8453816625763	40.0	37.0	41.0	33.0	41.0
28	37.851434145113586	40.0	37.0	41.0	33.0	41.0
29	37.78231200050343	40.0	37.0	41.0	33.0	41.0
30	37.70187653388711	40.0	37.0	41.0	33.0	41.0
31	37.70897866717009	40.0	37.0	41.0	33.0	41.0
32	37.6480636838462	40.0	37.0	41.0	33.0	41.0
33	37.54818198980555	40.0	37.0	41.0	32.0	41.0
34	37.5804983953181	40.0	37.0	41.0	33.0	41.0
35	37.4940318419231	40.0	37.0	41.0	32.0	41.0
36	37.4553533446605	40.0	37.0	41.0	32.0	41.0
37	37.36492731734944	39.0	36.0	41.0	32.0	41.0
38	37.30788496633314	39.0	36.0	41.0	32.0	41.0
39	37.20057768548235	39.0	36.0	41.0	31.0	41.0
40	37.099504121829966	39.0	36.0	41.0	31.0	41.0
41	37.13980492102448	39.0	36.0	41.0	31.0	41.0
42	37.093270404631554	39.0	36.0	41.0	31.0	41.0
43	37.04382606506828	39.0	36.0	41.0	31.0	41.0
44	36.98382606506828	39.0	36.0	41.0	31.0	41.0
45	36.77991567553961	39.0	35.0	40.0	31.0	41.0
46	36.61595368447549	39.0	35.0	40.0	30.0	41.0
47	36.557362028821345	39.0	35.0	40.0	30.0	41.0
48	36.527876156314896	39.0	35.0	40.0	30.0	41.0
49	36.41863444717136	39.0	35.0	40.0	30.0	41.0
50	36.23923982128249	39.0	35.0	40.0	30.0	41.0
51	34.961721729280725	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	7.0
13	3.0
14	6.0
15	11.0
16	27.0
17	61.0
18	131.0
19	268.0
20	489.0
21	762.0
22	1148.0
23	1590.0
24	2159.0
25	2853.0
26	3832.0
27	5056.0
28	6771.0
29	9199.0
30	12350.0
31	16590.0
32	21901.0
33	28747.0
34	41047.0
35	53625.0
36	70270.0
37	105563.0
38	176756.0
39	233268.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.874394311245357	33.54250833805298	21.33861934428293	25.24447800641873
2	20.83808445031779	32.55352086086464	21.230633692026935	25.377760996790638
3	20.057516833427727	31.977219809955322	22.201371845698823	25.76389151091813
4	19.938959159272542	31.558114656094645	20.441130199483982	28.061795985148823
5	18.3680070480146	33.91806683028129	19.408470203259707	28.3054559184444
6	20.783714051979107	36.20363727896294	19.177647725127432	23.835000943930527
7	86.80523566798817	3.7350701655024854	5.120634321313951	4.3390598451953935
8	88.86489207727645	3.071172361714178	3.6236863633503242	4.440249197659052
9	80.63079730665157	8.271600276886288	7.280221509030269	3.81738090743188
10	29.059593480586493	46.278648291485744	12.366748473978982	12.295009753948776
11	20.467686111635516	26.184381096217983	35.480586495500596	17.8673462966459
12	24.73953810332893	23.420552514001635	33.49984267824555	18.340066704423887
13	24.65483607073186	24.87307280850796	30.35013529670883	20.121955824051348
14	18.319803662450443	28.828267572839973	31.429614247058087	21.4223145176515
15	18.15920961550563	31.196526335661694	30.42149644452835	20.222767604304323
16	20.996916493612737	28.67975583663709	30.282172298785476	20.0411553709647
17	19.98137310427286	29.391101881568183	30.14674973255302	20.48077528160594
18	20.01850103832358	28.761311434145114	28.050594676231828	23.169592851299477
19	20.255238814423258	30.779560757661567	30.160090617330564	18.805109810584607
20	23.439682839343025	29.22043924233843	28.82147127304764	18.518406645270908
21	21.649864703291172	28.467686111635516	28.72758164999056	21.15486753508275
22	20.313636649675917	27.033163425838524	29.719086275250138	22.934113649235417
23	21.289786671700963	27.95305518847146	27.592977156881254	23.164180982946323
24	18.818324837958592	29.542634195456547	29.182304449059217	22.456736517525645
25	19.922723554213075	30.095651626706942	27.26486690579573	22.71675791328425
26	21.93065257063747	31.15562267950412	26.266691838147377	20.64703291171103
27	19.363539110188157	29.41388207161286	28.426782455477944	22.79579636272104
28	20.648669057957335	28.033603926750988	30.292240891070417	21.025486124221256
29	21.5533320747593	27.552199358127243	26.884903404442767	24.009565162670693
30	21.147190233465484	29.37587313573721	27.25605688754641	22.220879743250897
31	22.046944811528537	28.626014725316217	26.078409162418982	23.248631300736267
32	22.047322383739225	29.46070102573784	25.513938707444467	22.97803788307847
33	21.111195016046818	30.00088100182493	26.661758227927756	22.22616575420049
34	20.582468063683844	26.3150210811151	29.573846831539864	23.528664023661193
35	20.64690705430747	27.175759864074006	27.570952111257945	24.60638097036058
36	20.36863633503241	29.072179220942672	29.362028821345415	21.197155622679503
37	22.350135296708828	28.51437920835693	26.714366622616577	22.421118872317663
38	24.1412120067963	26.87470895475426	28.173557359511676	20.810521678937764
39	22.19759612359197	26.960417846579826	28.69108300295765	22.150903026870555
40	22.546095274054494	27.903215656661	28.45220565099742	21.09848341828708
41	19.08841482600214	26.26593669372601	31.86243785790699	22.783210622364862
42	22.040903656157575	27.16166383487509	27.845950538040398	22.95148197092694
43	21.032408281417155	26.60990497766031	28.426908312881505	23.93077842804103
44	23.59071172361714	26.283682587628217	26.91850733119376	23.207098357560884
45	24.002391290667674	25.89881064753634	27.68258762821723	22.416210433578758
46	19.559624944937386	27.51091812975898	29.81926876848531	23.110188156818325
47	20.6589893650494	27.029891133345917	28.65747907620666	23.653640425398024
48	21.173746145617017	24.84802718519917	31.286640236611916	22.691586432571896
49	21.475929771568815	24.732993518343715	28.900006292870177	24.891070417217293
50	20.072493864451577	24.679126549619284	27.923478698634447	27.324900887294696
51	19.701340381347933	24.662513372349128	31.013781385690013	24.622364860612926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1275.0
1	1033.5
2	792.0
3	555.5
4	319.0
5	299.5
6	280.0
7	314.0
8	348.0
9	409.5
10	471.0
11	538.0
12	605.0
13	659.5
14	714.0
15	822.5
16	931.0
17	1061.0
18	1191.0
19	1524.0
20	1857.0
21	2162.5
22	2468.0
23	3303.0
24	4138.0
25	5200.5
26	7670.5
27	9078.0
28	11089.0
29	13100.0
30	15780.0
31	18460.0
32	22347.5
33	26235.0
34	28289.0
35	30343.0
36	31911.5
37	33480.0
38	37511.0
39	41542.0
40	41423.0
41	41304.0
42	44988.0
43	48672.0
44	56332.0
45	63992.0
46	70175.5
47	76359.0
48	81854.0
49	87349.0
50	80182.0
51	73015.0
52	64910.0
53	56805.0
54	48281.0
55	39757.0
56	35191.5
57	30626.0
58	28053.0
59	25480.0
60	22726.5
61	19973.0
62	18397.5
63	16822.0
64	13102.5
65	9383.0
66	7082.0
67	4781.0
68	3673.5
69	2566.0
70	1911.5
71	1257.0
72	1089.5
73	922.0
74	813.0
75	566.0
76	428.0
77	324.0
78	220.0
79	154.0
80	88.0
81	70.0
82	52.0
83	47.0
84	42.0
85	36.5
86	31.0
87	23.0
88	15.0
89	12.5
90	10.0
91	7.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	794550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.814632390957783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40394541373617	19.146705475333516
2	12.115971236680414	6.49770629542007
3	4.691522501216456	3.7740435367207814
4	2.348668028217779	2.5191467914022163
5	1.3490222185532061	1.8086767438869265
6	0.8487128654156847	1.3654754094958805
7	0.6331961719234148	1.188524580704165
8	0.47067283303696206	1.009673519543743
9	0.38114711391394446	0.9198287771829237
>10	4.247830930323256	27.61621983529207
>50	1.2771452677402473	23.930655367139682
>100	0.2260434102157888	8.208349906658606
>500	0.003767390170263147	0.6716645870731678
>1k	0.0018836950851315736	0.6935104177863955
>5k	4.709237712828934E-4	0.6498187563599401
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	5146	0.6476621987288402	TruSeq Adapter, Index 1 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	1809	0.227676043043232	RNA PCR Primer, Index 40 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	1428	0.17972437228619972	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGT	1208	0.15203574350261154	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1047	0.13177270152916745	No Hit
AAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	953	0.11994210559436158	RNA PCR Primer, Index 40 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	852	0.10723050783462337	RNA PCR Primer, Index 1 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5171480712352903E-4	0.0	0.0	0.7458309735070165	0.0
2	2.5171480712352903E-4	0.0	0.0	1.042225158894972	0.0
3	2.5171480712352903E-4	0.0	0.0	1.3877037316720156	0.0
4	2.5171480712352903E-4	0.0	0.0	3.132213202441634	0.0
5	2.5171480712352903E-4	0.0	0.0	3.3728525580517275	0.0
6	2.5171480712352903E-4	0.0	0.0	4.738153671889749	0.0
7	2.5171480712352903E-4	0.0	0.0	5.731672015606318	0.0
8	2.5171480712352903E-4	0.0	0.0	6.456862374929205	0.0
9	2.5171480712352903E-4	0.0	0.0	7.833238940280662	0.0
10	2.5171480712352903E-4	0.0	0.0	9.706500534893966	0.0
11	2.5171480712352903E-4	0.0	0.0	11.593228871688376	0.0
12	2.5171480712352903E-4	0.0	0.0	12.499528034736644	0.0
13	2.5171480712352903E-4	0.0	0.0	12.87168837706878	0.0
14	2.5171480712352903E-4	0.0	0.0	13.276319929519854	0.0
15	2.5171480712352903E-4	0.0	0.0	13.63476181486376	0.0
16	2.5171480712352903E-4	0.0	0.0	14.34812157825184	0.0
17	2.5171480712352903E-4	0.0	0.0	15.34667421811088	0.0
18	2.5171480712352903E-4	0.0	0.0	16.446038638222895	0.0
19	2.5171480712352903E-4	0.0	0.0	17.03001699074948	0.0
20	2.5171480712352903E-4	0.0	0.0	17.6277138002643	0.0
21	2.5171480712352903E-4	0.0	0.0	18.327103391857026	0.0
22	2.5171480712352903E-4	0.0	0.0	19.182178591655653	0.0
23	2.5171480712352903E-4	0.0	0.0	19.941098735133096	0.0
24	2.5171480712352903E-4	0.0	0.0	20.509344912214463	0.0
25	2.5171480712352903E-4	0.0	0.0	20.980555031149706	0.0
26	2.5171480712352903E-4	0.0	0.0	21.434774400604116	0.0
27	2.5171480712352903E-4	0.0	0.0	21.929519854005413	0.0
28	2.5171480712352903E-4	0.0	0.0	22.43093574979548	0.0
29	2.5171480712352903E-4	0.0	0.0	22.988358190170537	0.0
30	2.5171480712352903E-4	0.0	0.0	23.616512491347304	0.0
31	2.5171480712352903E-4	0.0	0.0	24.15052545465987	0.0
32	2.5171480712352903E-4	0.0	0.0	24.63696431942609	0.0
33	2.5171480712352903E-4	0.0	0.0	25.094078409162417	0.0
34	2.5171480712352903E-4	0.0	0.0	25.59964759927003	0.0
35	2.5171480712352903E-4	0.0	0.0	26.113271663205587	0.0
36	2.5171480712352903E-4	0.0	0.0	26.63193002328362	0.0
37	2.5171480712352903E-4	0.0	0.0	27.1484488075011	0.0
38	2.5171480712352903E-4	0.0	0.0	27.658423006733372	0.0
39	2.5171480712352903E-4	0.0	0.0	28.15342017494179	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACCG	30	2.164652E-6	45.000004	1
CGAAACG	35	1.2113014E-7	45.000004	1
CCGGCCG	30	2.164652E-6	45.000004	1
CGCGACG	30	2.164652E-6	45.000004	1
CGACAAG	35	1.2113014E-7	45.000004	1
TGACCTA	30	2.164652E-6	45.000004	28
CGGATCA	35	1.2113014E-7	45.000004	6
CTACGCG	30	2.164652E-6	45.000004	1
CACTACG	65	0.0	45.000004	1
CTTGCGT	30	2.164652E-6	45.000004	18
CGCTACG	60	0.0	45.000004	1
AAACCCG	35	1.2113014E-7	45.000004	1
ACGTGAC	30	2.164652E-6	45.000004	37
GAACGTA	35	1.2113014E-7	45.000004	9
CGTACTA	35	1.2113014E-7	45.000004	12
CGAGCAA	30	2.164652E-6	45.000004	1
ACGTACG	30	2.164652E-6	45.000004	1
ACCAACG	30	2.164652E-6	45.000004	1
CGCGTTG	35	1.2113014E-7	45.000004	1
CGCGTAG	35	1.2113014E-7	45.000004	1
>>END_MODULE