FastQCFastQC Report
Sat 18 Jun 2016
SRR3554078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647466
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT46430.7171032919103087TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC10790.16664967735757552TruSeq Adapter, Index 21 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA7760.11985185322472532No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT7480.11552730181970945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7030.10857712991879111No Hit
AAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT6620.10224475107573215No Hit
TACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT6530.10085471669554849No Hit
AACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT6480.10008247537322423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG302.1640262E-645.0000041
CAGCGCG950.045.0000041
CGAACAC207.0310006E-445.00000429
CACGGTT207.0310006E-445.00000425
TTTCGTA207.0310006E-445.00000445
TATGGCG207.0310006E-445.0000041
ATAGCCG207.0310006E-445.00000410
ATTCGCG406.8066583E-945.0000041
TAACGAG302.1640262E-645.0000041
TCGATGG302.1640262E-645.0000042
ATCCGTC207.0310006E-445.00000427
ATCGTAG207.0310006E-445.0000041
CGCCTCA207.0310006E-445.00000439
TACGATT207.0310006E-445.00000425
AATTGCG207.0310006E-445.0000041
ATATACG302.1640262E-645.0000041
GTACTAC406.8066583E-945.00000434
CAGTGCG406.8066583E-945.0000041
GCGATCC207.0310006E-445.00000424
GTATCGG207.0310006E-445.0000042