Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554078_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 647466 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT | 4643 | 0.7171032919103087 | TruSeq Adapter, Index 14 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC | 1079 | 0.16664967735757552 | TruSeq Adapter, Index 21 (95% over 21bp) |
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA | 776 | 0.11985185322472532 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT | 748 | 0.11552730181970945 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 703 | 0.10857712991879111 | No Hit |
AAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT | 662 | 0.10224475107573215 | No Hit |
TACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT | 653 | 0.10085471669554849 | No Hit |
AACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT | 648 | 0.10008247537322423 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTAGACG | 30 | 2.1640262E-6 | 45.000004 | 1 |
CAGCGCG | 95 | 0.0 | 45.000004 | 1 |
CGAACAC | 20 | 7.0310006E-4 | 45.000004 | 29 |
CACGGTT | 20 | 7.0310006E-4 | 45.000004 | 25 |
TTTCGTA | 20 | 7.0310006E-4 | 45.000004 | 45 |
TATGGCG | 20 | 7.0310006E-4 | 45.000004 | 1 |
ATAGCCG | 20 | 7.0310006E-4 | 45.000004 | 10 |
ATTCGCG | 40 | 6.8066583E-9 | 45.000004 | 1 |
TAACGAG | 30 | 2.1640262E-6 | 45.000004 | 1 |
TCGATGG | 30 | 2.1640262E-6 | 45.000004 | 2 |
ATCCGTC | 20 | 7.0310006E-4 | 45.000004 | 27 |
ATCGTAG | 20 | 7.0310006E-4 | 45.000004 | 1 |
CGCCTCA | 20 | 7.0310006E-4 | 45.000004 | 39 |
TACGATT | 20 | 7.0310006E-4 | 45.000004 | 25 |
AATTGCG | 20 | 7.0310006E-4 | 45.000004 | 1 |
ATATACG | 30 | 2.1640262E-6 | 45.000004 | 1 |
GTACTAC | 40 | 6.8066583E-9 | 45.000004 | 34 |
CAGTGCG | 40 | 6.8066583E-9 | 45.000004 | 1 |
GCGATCC | 20 | 7.0310006E-4 | 45.000004 | 24 |
GTATCGG | 20 | 7.0310006E-4 | 45.000004 | 2 |