##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647466
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858471332857633	33.0	31.0	34.0	30.0	34.0
2	32.06515863381243	34.0	31.0	34.0	30.0	34.0
3	32.21954048552357	34.0	31.0	34.0	30.0	34.0
4	35.84018929179293	37.0	35.0	37.0	35.0	37.0
5	35.641987378487826	37.0	35.0	37.0	33.0	37.0
6	35.653237390071446	37.0	35.0	37.0	33.0	37.0
7	35.79888828139238	37.0	35.0	37.0	35.0	37.0
8	35.98026768973197	37.0	35.0	37.0	35.0	37.0
9	37.627115555102506	39.0	37.0	39.0	35.0	39.0
10	37.08762313387884	39.0	37.0	39.0	33.0	39.0
11	37.08393954277136	39.0	37.0	39.0	33.0	39.0
12	37.11723858858998	39.0	37.0	39.0	33.0	39.0
13	37.089091936873906	39.0	37.0	39.0	33.0	39.0
14	38.27033388625812	40.0	38.0	41.0	33.0	41.0
15	38.250482650826456	40.0	38.0	41.0	33.0	41.0
16	38.18019942359908	40.0	37.0	41.0	33.0	41.0
17	38.193571554336444	40.0	37.0	41.0	33.0	41.0
18	38.15931801824343	40.0	37.0	41.0	33.0	41.0
19	38.18624143970494	40.0	37.0	41.0	34.0	41.0
20	38.114722626361846	40.0	37.0	41.0	33.0	41.0
21	38.0822838573763	40.0	37.0	41.0	33.0	41.0
22	38.08924175169044	40.0	37.0	41.0	33.0	41.0
23	37.98766421711719	40.0	37.0	41.0	33.0	41.0
24	37.882010792844724	40.0	37.0	41.0	33.0	41.0
25	37.92248550503038	40.0	37.0	41.0	33.0	41.0
26	37.87709007113887	40.0	37.0	41.0	33.0	41.0
27	37.858086756679114	40.0	37.0	41.0	33.0	41.0
28	37.82800023476136	40.0	37.0	41.0	33.0	41.0
29	37.750467206	40.0	37.0	41.0	33.0	41.0
30	37.71269533844248	40.0	37.0	41.0	33.0	41.0
31	37.63331819740342	40.0	37.0	41.0	33.0	41.0
32	37.65262114149623	40.0	37.0	41.0	33.0	41.0
33	37.587346671485456	40.0	37.0	41.0	33.0	41.0
34	37.59456712784918	40.0	37.0	41.0	33.0	41.0
35	37.558540525680115	40.0	37.0	41.0	33.0	41.0
36	37.48199905477662	40.0	37.0	41.0	32.0	41.0
37	37.40327831886153	40.0	36.0	41.0	32.0	41.0
38	37.38969922744978	39.0	36.0	41.0	32.0	41.0
39	37.330326225624205	39.0	36.0	41.0	32.0	41.0
40	37.25032356911405	39.0	36.0	41.0	32.0	41.0
41	37.23347480794358	39.0	36.0	41.0	32.0	41.0
42	37.167103755255106	39.0	36.0	41.0	31.0	41.0
43	37.09915733026908	39.0	36.0	41.0	31.0	41.0
44	36.99315176395363	39.0	36.0	41.0	31.0	41.0
45	36.79785656698576	39.0	35.0	40.0	31.0	41.0
46	36.69648908205218	39.0	35.0	40.0	30.0	41.0
47	36.627220579922344	39.0	35.0	40.0	30.0	41.0
48	36.57819715629856	39.0	35.0	40.0	30.0	41.0
49	36.43942384619424	39.0	35.0	40.0	30.0	41.0
50	36.322348972764594	39.0	35.0	40.0	30.0	41.0
51	35.04746195167005	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	2.0
13	7.0
14	6.0
15	13.0
16	24.0
17	50.0
18	126.0
19	229.0
20	373.0
21	648.0
22	942.0
23	1313.0
24	1834.0
25	2414.0
26	3058.0
27	4066.0
28	5468.0
29	7452.0
30	9910.0
31	13210.0
32	17441.0
33	23620.0
34	34039.0
35	43501.0
36	56853.0
37	85093.0
38	144028.0
39	191670.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.992246697123864	32.56510766588516	20.763252433332408	26.67939320365857
2	21.457034037308524	31.110205014626253	22.03559723599386	25.397163712071368
3	20.83785094506893	30.59450225957811	21.992815066737094	26.574831728615866
4	20.02622531530613	30.67342532271965	21.146438577469706	28.153910784504514
5	18.517265771484524	33.36638526192881	19.115907244550293	29.000441722036367
6	21.42351876391965	35.044929000132825	19.783123747038456	23.74842848890907
7	85.67770353964532	3.9412108126140986	5.7337373699931735	4.647348277747403
8	87.32952772809692	3.645596834428372	4.361155643694032	4.663719793780677
9	78.77849956600038	8.464846030525155	8.509018234162102	4.247636169312365
10	28.375544044011576	44.91957878869315	13.252433332406643	13.452443834888628
11	19.667596445218745	25.132284938514143	37.175079463632066	18.025039152635042
12	23.64788266874245	23.20708732195976	34.46049676739783	18.684533241899963
13	23.239675905761846	24.356800202636123	31.334463894629216	21.069059996972815
14	18.418882227020415	27.09609462118474	32.92991446655114	21.555108685243702
15	17.804178134450304	30.78771703842364	30.827873587184502	20.580231239941558
16	20.90596262969793	28.673165849635346	29.864888658246148	20.555982862420574
17	20.433505388699945	29.53452382055583	29.446179413281932	20.585791377462293
18	19.41847139463694	28.59455168302274	29.152573262534247	22.834403659806075
19	19.778335850840044	31.370141443720595	30.143976672134137	18.70754603330522
20	21.54630513416921	29.683875292293337	29.726039668492245	19.04377990504521
21	20.40215239101358	29.466412135926827	29.229334049973282	20.902101423086307
22	19.85077208687406	26.85623646647083	31.304500931323037	21.988490515332078
23	20.53188893316406	28.753942291950462	28.064794135908294	22.649374638977182
24	17.87630547395539	29.20956467212178	30.466310200072282	22.44781965385055
25	19.481022941745206	29.563096749481826	28.266966914092784	22.688913394680185
26	20.948744798954692	30.48885964668415	28.071126514751356	20.4912690396098
27	19.39375967232256	28.803829081372612	28.687375089966118	23.11503615633871
28	19.915949254478228	27.122968619201625	31.880901854305865	21.080180272014285
29	20.697611920934843	26.522010422168883	30.78709924536578	21.99327841153049
30	22.57894623038121	26.91585349655426	29.102532024847637	21.402668248216894
31	20.49065124655194	28.04487030979233	29.156125572616943	22.30835287103879
32	22.021233547398626	26.142531036378745	29.110872231128738	22.72536318509389
33	21.91188417615751	26.187012136544624	29.41899651873612	22.482107168561747
34	18.49919532454214	24.845937856196308	32.92991446655114	23.724952352710414
35	21.133001578461265	24.503217157348804	31.444431058928192	22.919350205261743
36	20.806652395647028	26.842027226140058	31.945924573645566	20.405395804567345
37	21.11508557978334	26.789669264486477	29.892226001056425	22.203019154673758
38	21.338417770199516	24.740449691566816	32.44402022654471	21.477112311688952
39	20.989055795980022	24.788483101815384	30.576586260900186	23.64587484130441
40	22.73463008096178	25.81031281951485	27.891812079707663	23.563245019815714
41	19.248423855461137	24.184127042964416	31.389910821572098	25.17753828000235
42	21.427071074002342	25.707141378852327	28.912097314762477	23.95369023238286
43	20.77483605316727	25.275149583144135	29.8404858324607	24.109528531227895
44	22.57477612724066	24.133931357013342	29.43969258617441	23.85159992957159
45	22.708373876002756	24.733808416194826	30.525000540568925	22.032817167233492
46	19.69385265017777	24.530400051894617	32.399230229849906	23.376517068077707
47	21.174548161602306	24.75296000098847	29.503788615927323	24.5687032214819
48	20.867659460110648	23.168011911050154	32.8791009875422	23.085227641296996
49	21.043267136807184	23.625642118659513	30.65350149660368	24.67758924792962
50	20.147930547704433	22.52998613054585	29.600936574275714	27.721146747473995
51	19.634235620094337	21.79280456425511	33.31866074820917	25.254299067441377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	978.0
1	803.5
2	629.0
3	470.5
4	312.0
5	316.0
6	320.0
7	343.0
8	366.0
9	404.0
10	442.0
11	495.5
12	549.0
13	662.0
14	775.0
15	849.5
16	924.0
17	1075.0
18	1226.0
19	1459.5
20	1693.0
21	2124.0
22	2555.0
23	2914.0
24	3273.0
25	4253.5
26	6151.5
27	7069.0
28	8396.5
29	9724.0
30	12146.0
31	14568.0
32	17764.0
33	20960.0
34	21922.5
35	22885.0
36	25673.0
37	28461.0
38	31554.5
39	34648.0
40	36748.5
41	38849.0
42	40728.0
43	42607.0
44	46949.0
45	51291.0
46	56432.0
47	61573.0
48	67142.5
49	72712.0
50	67108.5
51	61505.0
52	53391.0
53	45277.0
54	39624.5
55	33972.0
56	28957.0
57	23942.0
58	20794.0
59	17646.0
60	15915.5
61	14185.0
62	12092.5
63	10000.0
64	8440.0
65	6880.0
66	5334.0
67	3788.0
68	2905.0
69	2022.0
70	1661.5
71	1301.0
72	1057.5
73	814.0
74	824.5
75	579.5
76	324.0
77	248.0
78	172.0
79	128.0
80	84.0
81	60.5
82	37.0
83	39.0
84	41.0
85	25.0
86	9.0
87	6.5
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	647466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.2223886955858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96590598627591	21.030156775609395
2	11.905012000176153	6.957857761895219
3	4.435403542085291	3.888392589857833
4	2.248456371850661	2.628210642531464
5	1.307304659623715	1.9101282453537345
6	0.8105244493443969	1.4211276303610565
7	0.6022570205344515	1.23195721240922
8	0.4914265719376894	1.1488526640401933
9	0.39697784603721287	1.0440576828392265
>10	4.722481327808197	33.083036951347886
>50	1.0221021544438498	19.852028408662086
>100	0.08632261718877332	4.004514313463756
>500	0.004766279476680736	0.9141546626074513
>1k	0.0010591732170401635	0.885524459021472
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	4643	0.7171032919103087	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	1079	0.16664967735757552	TruSeq Adapter, Index 21 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	776	0.11985185322472532	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	748	0.11552730181970945	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.10857712991879111	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	662	0.10224475107573215	No Hit
TACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	653	0.10085471669554849	No Hit
AACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	648	0.10008247537322423	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8040576648040206	0.0
2	0.0	0.0	0.0	1.1824559127429084	0.0
3	0.0	0.0	0.0	1.5798512972109733	0.0
4	0.0	0.0	0.0	3.4374005739297506	0.0
5	0.0	0.0	0.0	3.7039782783960855	0.0
6	0.0	0.0	0.0	5.237495096267604	0.0
7	0.0	0.0	0.0	6.370682012646224	0.0
8	0.0	0.0	0.0	7.264783015633253	0.0
9	0.0	0.0	0.0	8.74285290656189	0.0
10	0.0	0.0	0.0	10.777245446092921	0.0
11	0.0	0.0	0.0	12.779049401821872	0.0
12	0.0	0.0	0.0	13.776630742000352	0.0
13	0.0	0.0	0.0	14.156727920848354	0.0
14	0.0	0.0	0.0	14.594743198870674	0.0
15	0.0	0.0	0.0	14.946885241850538	0.0
16	0.0	0.0	0.0	15.671402050455159	0.0
17	0.0	0.0	0.0	16.731843834270833	0.0
18	0.0	0.0	0.0	17.853292682550126	0.0
19	0.0	0.0	0.0	18.457803189665558	0.0
20	0.0	0.0	0.0	19.05397349049989	0.0
21	0.0	0.0	0.0	19.763045472658025	0.0
22	0.0	0.0	0.0	20.620696685231348	0.0
23	0.0	0.0	0.0	21.385833387390225	0.0
24	0.0	0.0	0.0	21.95451189714981	0.0
25	0.0	0.0	0.0	22.450908619139845	0.0
26	0.0	0.0	0.0	22.929852687245354	0.0
27	0.0	0.0	0.0	23.467178199318575	0.0
28	0.0	0.0	0.0	23.982263161308857	0.0
29	0.0	0.0	0.0	24.552949498506486	0.0
30	0.0	0.0	0.0	25.219857104465717	0.0
31	0.0	0.0	0.0	25.724902929265784	0.0
32	0.0	0.0	0.0	26.21682065158634	0.0
33	0.0	0.0	0.0	26.67877541060071	0.0
34	0.0	0.0	0.0	27.16575078845839	0.0
35	0.0	0.0	0.0	27.688249267142986	0.0
36	0.0	0.0	0.0	28.209666607976327	0.0
37	0.0	0.0	0.0	28.696950882362934	0.0
38	0.0	0.0	0.0	29.189331949476884	0.0
39	0.0	0.0	0.0	29.70966815245897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1640262E-6	45.000004	1
CAGCGCG	95	0.0	45.000004	1
CGAACAC	20	7.0310006E-4	45.000004	29
CACGGTT	20	7.0310006E-4	45.000004	25
TTTCGTA	20	7.0310006E-4	45.000004	45
TATGGCG	20	7.0310006E-4	45.000004	1
ATAGCCG	20	7.0310006E-4	45.000004	10
ATTCGCG	40	6.8066583E-9	45.000004	1
TAACGAG	30	2.1640262E-6	45.000004	1
TCGATGG	30	2.1640262E-6	45.000004	2
ATCCGTC	20	7.0310006E-4	45.000004	27
ATCGTAG	20	7.0310006E-4	45.000004	1
CGCCTCA	20	7.0310006E-4	45.000004	39
TACGATT	20	7.0310006E-4	45.000004	25
AATTGCG	20	7.0310006E-4	45.000004	1
ATATACG	30	2.1640262E-6	45.000004	1
GTACTAC	40	6.8066583E-9	45.000004	34
CAGTGCG	40	6.8066583E-9	45.000004	1
GCGATCC	20	7.0310006E-4	45.000004	24
GTATCGG	20	7.0310006E-4	45.000004	2
>>END_MODULE