FastQCFastQC Report
Sat 18 Jun 2016
SRR3554075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343969
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT49081.426872770511296No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCC35561.0338140937119333No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT32700.9506670659274528No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT29710.8637406277891322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9290.2700824783628757No Hit
CTGCTAGGGCAAAGGGCAGTCCAGAGTTATAACACAAAAATAATGACTCTG9190.2671752396291526No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT8910.25903497117472796No Hit
AATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT7900.2296718599641247No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC7420.2157171140422538No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG6850.19914585326003217No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT6810.19798295776654293No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT6090.17705083888373663No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT5800.16861984655593962No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4650.13518660111812403No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTTC4610.13402370562463478No Hit
AACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT3980.11570810160217927No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT3760.10931217638798846No Hit
ACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG3710.1078585570211269No Hit
TACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT3440.10000901244007455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC302.1610504E-645.00000436
ACCGGGT600.045.0000044
CGAACAC351.2086093E-745.00000424
ATTAGCG302.1610504E-645.0000041
AATCACG302.1610504E-645.0000041
CATGCGG351.2086093E-745.0000042
CTATCCG302.1610504E-645.0000041
TTTCGAA351.2086093E-745.00000421
AGCTTAG302.1610504E-645.0000041
ATAGCGG351.2086093E-745.0000042
TCCTCGG351.2086093E-745.0000042
TTCCGCG351.2086093E-745.00000440
ACGGGTC302.1610504E-645.0000045
TCTAGCG351.2086093E-745.0000041
TCAGGTA302.1610504E-645.00000412
CATTAGA302.1610504E-645.00000425
GGCCATA302.1610504E-645.00000414
CCACTCA302.1610504E-645.00000443
TACGAAT351.2086093E-745.00000411
TAACACG302.1610504E-645.0000041
Produced by FastQC (version 0.11.5)