##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343969
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63353965037547	31.0	31.0	34.0	30.0	34.0
2	31.830080617730086	33.0	31.0	34.0	30.0	34.0
3	31.98366422555521	33.0	31.0	34.0	30.0	34.0
4	35.604949864668036	37.0	35.0	37.0	33.0	37.0
5	35.41663638292986	37.0	35.0	37.0	33.0	37.0
6	35.399794748945396	37.0	35.0	37.0	33.0	37.0
7	35.439225627890885	37.0	35.0	37.0	33.0	37.0
8	35.73033907125352	37.0	35.0	37.0	35.0	37.0
9	37.35470929066282	39.0	37.0	39.0	34.0	39.0
10	36.85042547438868	39.0	37.0	39.0	32.0	39.0
11	36.90709046454768	39.0	37.0	39.0	33.0	39.0
12	36.86143809471202	39.0	37.0	39.0	33.0	39.0
13	36.80592146385285	39.0	37.0	39.0	33.0	39.0
14	37.91316659350116	40.0	37.0	41.0	33.0	41.0
15	37.94573929627379	40.0	37.0	41.0	33.0	41.0
16	37.86261843363791	40.0	37.0	41.0	33.0	41.0
17	37.86705197270684	40.0	37.0	41.0	33.0	41.0
18	37.77146487038076	39.0	37.0	41.0	33.0	41.0
19	37.75214917623391	39.0	37.0	41.0	32.0	41.0
20	37.714738828208354	39.0	37.0	41.0	33.0	41.0
21	37.74134878433812	39.0	37.0	41.0	33.0	41.0
22	37.703813425047024	39.0	37.0	41.0	33.0	41.0
23	37.590454372341696	39.0	36.0	41.0	32.0	41.0
24	37.49297175036122	39.0	36.0	41.0	32.0	41.0
25	37.519828821783356	39.0	36.0	41.0	32.0	41.0
26	37.46075082347537	39.0	36.0	41.0	32.0	41.0
27	37.340364974750635	39.0	36.0	41.0	32.0	41.0
28	37.38427300134605	39.0	36.0	41.0	32.0	41.0
29	37.16867217685315	39.0	36.0	41.0	31.0	41.0
30	37.10297439594847	39.0	36.0	41.0	31.0	41.0
31	37.09162453593202	39.0	36.0	40.0	31.0	41.0
32	36.891141352854476	39.0	36.0	40.0	31.0	41.0
33	36.873860725821224	39.0	36.0	40.0	31.0	41.0
34	36.86190616014815	39.0	35.0	40.0	31.0	41.0
35	36.766743514677195	39.0	35.0	40.0	30.0	41.0
36	36.762987362233225	39.0	35.0	40.0	30.0	41.0
37	36.59491407655923	39.0	35.0	40.0	30.0	41.0
38	36.539167773840084	39.0	35.0	40.0	30.0	41.0
39	36.37469364971843	39.0	35.0	40.0	30.0	41.0
40	36.38854373504589	39.0	35.0	40.0	30.0	41.0
41	36.52337856027724	39.0	35.0	40.0	30.0	41.0
42	36.45190118876992	39.0	35.0	40.0	30.0	41.0
43	36.40708028921211	39.0	35.0	40.0	30.0	41.0
44	36.304411153330676	39.0	35.0	40.0	30.0	41.0
45	36.080106056069006	38.0	35.0	40.0	28.0	41.0
46	35.80053435047926	38.0	34.0	40.0	28.0	41.0
47	35.787637839456465	38.0	34.0	40.0	28.0	41.0
48	35.815620012268546	38.0	35.0	40.0	28.0	41.0
49	35.6770435707869	38.0	34.0	40.0	28.0	41.0
50	35.546479479255396	38.0	34.0	40.0	28.0	41.0
51	34.23509095296379	37.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	8.0
15	12.0
16	25.0
17	53.0
18	93.0
19	225.0
20	409.0
21	635.0
22	909.0
23	1203.0
24	1510.0
25	1849.0
26	2275.0
27	2968.0
28	3797.0
29	5081.0
30	6621.0
31	8581.0
32	11310.0
33	15014.0
34	20950.0
35	27171.0
36	33555.0
37	47348.0
38	71821.0
39	80523.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.82573429582317	35.332835226430284	19.954995944401965	26.886434533344573
2	21.214121039977442	34.95111478069244	21.001020440795536	22.833743738534576
3	20.58441313025302	31.233919335754095	23.994894888783584	24.186772645209306
4	22.002564184563145	29.34799356918792	20.57481924243173	28.074623003817205
5	17.04921083004573	36.32682014949021	19.485186165032314	27.138782855431735
6	21.49844898813556	33.83299076370254	23.364896255185787	21.30366399297611
7	78.22245609342703	7.574229072968786	8.060319389247287	6.142995444356904
8	79.9150504842006	4.458541322037742	6.331675238175532	9.294732955586115
9	73.28509255194508	9.268277083109233	11.624303352918432	5.822327012027246
10	28.008628684561693	40.23647479860104	15.026063395247826	16.728833121589446
11	19.909061572409144	24.570237434187383	35.85701037012056	19.66369062328291
12	22.72181504728624	24.11554529623309	35.87445380252291	17.288185853957767
13	24.126302079547866	25.61975061706142	30.01607703019749	20.237870273193224
14	17.17218702848222	26.190150856617894	35.15055135782585	21.48711075707404
15	16.026734967395317	30.442278228561293	30.865281464318006	22.66570533972538
16	18.300777104913525	29.256996996822387	30.56147501664394	21.880750881620145
17	18.022845081969596	28.39122130191965	32.18778436428864	21.39814925182211
18	17.618738897982087	26.784390453790895	30.60275780666281	24.994112841564213
19	18.18710407042495	30.947265596608997	33.01809174663996	17.847538586326092
20	20.29310780913396	30.60217635891607	30.398378923682074	18.706336908267897
21	19.332846855385224	29.148266268181146	28.68601530951917	22.832871566914463
22	17.298942637272543	28.110672764115368	31.529585514973736	23.06079908363835
23	20.63877849457364	28.201669336480904	27.05970596187447	24.099846207070986
24	15.962194267506666	31.54935473836305	27.43503048239812	25.05342051173216
25	17.248647407179135	28.726716651791296	28.21504263465603	25.80959330637354
26	22.598257401103005	29.01947559227721	26.5029697443665	21.87929726225328
27	15.803459032645383	29.79396399094104	29.45701502170254	24.945561954711035
28	21.305699060089715	25.31245548290689	28.395291436146863	24.98655402085653
29	22.6014553637101	29.69715294110806	26.33057048745672	21.370821207725115
30	20.58441313025302	30.49984155548901	24.525175233814675	24.390570080443297
31	24.1658405263265	29.825943617011998	22.148507568996042	23.859708287665455
32	21.627530387912863	30.495771421261804	23.29076166747585	24.58593652334949
33	21.64235730545485	30.302440045469215	24.272536187854136	23.782666461221798
34	20.067215359523676	26.596001383845636	24.604542851245313	28.73224040538537
35	21.765042780017968	27.987987289552258	26.514017251554645	23.73295267887513
36	22.49185246344874	28.724390860804316	28.076948794804185	20.70680788094276
37	20.26403542179673	31.87438402879329	24.306260157165326	23.55532039224465
38	22.679660085647253	30.32104637336504	28.233358238678484	18.76593530230922
39	19.18893853806593	28.905221110041897	28.00106986385401	23.904770488038167
40	19.595370513040418	29.854434556602484	26.75095720835308	23.79923772200402
41	18.372295177763114	23.91029424163224	32.36192796443866	25.355482616165993
42	22.523832089519697	25.410138704359987	28.3644747055694	23.701554500550923
43	19.513386380749427	24.940910372737076	30.18033601865284	25.365367227860652
44	21.461527056217275	24.783338033369287	26.630597524776945	27.1245373856365
45	25.09935488372499	25.756972285293152	27.147795295506285	21.99587753547558
46	18.13884390744515	26.793402893865437	32.246219862836476	22.82153333585294
47	20.453005939488733	25.63079812424957	27.292575784445695	26.623620151816006
48	20.120708552224183	23.16255243931866	33.84723623349778	22.869502774959372
49	21.84470112132198	22.31044076646442	30.21057130148356	25.63428681073004
50	19.766897598330083	22.115946495178346	27.385316700051458	30.731839206440114
51	19.5273411266713	22.036578877747704	33.619308716773894	24.8167712788071
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1103.0
1	900.0
2	697.0
3	487.5
4	278.0
5	265.5
6	253.0
7	261.5
8	270.0
9	317.0
10	364.0
11	438.5
12	513.0
13	553.5
14	594.0
15	613.0
16	632.0
17	744.5
18	857.0
19	832.0
20	807.0
21	1031.0
22	1255.0
23	1671.0
24	2087.0
25	2376.0
26	3322.5
27	3980.0
28	4853.0
29	5726.0
30	6349.0
31	6972.0
32	8298.0
33	9624.0
34	10004.5
35	10385.0
36	10710.0
37	11035.0
38	12894.5
39	14754.0
40	15305.5
41	15857.0
42	18225.0
43	20593.0
44	27699.0
45	34805.0
46	37011.0
47	39217.0
48	41414.0
49	43611.0
50	38837.5
51	34064.0
52	28585.0
53	23106.0
54	19963.0
55	16820.0
56	14221.0
57	11622.0
58	10562.5
59	9503.0
60	8035.0
61	6567.0
62	5870.5
63	5174.0
64	4118.0
65	3062.0
66	2457.5
67	1853.0
68	1565.5
69	1278.0
70	1140.0
71	1002.0
72	727.0
73	452.0
74	326.5
75	164.0
76	127.0
77	121.5
78	116.0
79	74.0
80	32.0
81	37.0
82	42.0
83	26.0
84	10.0
85	6.0
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	343969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.42946440018649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29511004529925	21.27606367385699
2	12.033376830537685	7.082716700966754
3	4.836075951624178	4.269693751647652
4	2.3822300020398375	2.8043101215235033
5	1.3845429955805444	2.03731793994826
6	0.91569742510891	1.6169090864151057
7	0.6707783416416204	1.3818453128030737
8	0.47884176652751265	1.1273645377075072
9	0.35688206814587514	0.9452563307607563
>10	3.4658547066062244	24.02092566139961
>50	1.017663712525265	20.661779447706
>100	0.14995044880038286	6.441452649222179
>500	0.008997026928022971	2.008191632043416
>1k	0.0039986786346768765	4.326173153999185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	4908	1.426872770511296	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCC	3556	1.0338140937119333	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	3270	0.9506670659274528	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	2971	0.8637406277891322	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	929	0.2700824783628757	No Hit
CTGCTAGGGCAAAGGGCAGTCCAGAGTTATAACACAAAAATAATGACTCTG	919	0.2671752396291526	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	891	0.25903497117472796	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	790	0.2296718599641247	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	742	0.2157171140422538	No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	685	0.19914585326003217	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	681	0.19798295776654293	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	609	0.17705083888373663	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	580	0.16861984655593962	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	465	0.13518660111812403	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTTC	461	0.13402370562463478	No Hit
AACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	398	0.11570810160217927	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	376	0.10931217638798846	No Hit
ACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	371	0.1078585570211269	No Hit
TACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	344	0.10000901244007455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5829914905122264	0.0
2	0.0	0.0	0.0	2.096119127014353	0.0
3	0.0	0.0	0.0	2.7624582447836867	0.0
4	0.0	0.0	0.0	6.090955871023261	0.0
5	0.0	0.0	0.0	6.487212510429719	0.0
6	0.0	0.0	0.0	8.492916512825284	0.0
7	0.0	0.0	0.0	10.084048271791934	0.0
8	0.0	0.0	0.0	11.216999206323825	0.0
9	0.0	0.0	0.0	13.778567254607246	0.0
10	0.0	0.0	0.0	16.42241015905503	0.0
11	0.0	0.0	0.0	19.76137384473601	0.0
12	0.0	0.0	0.0	21.041431059194288	0.0
13	0.0	0.0	0.0	21.612994194244248	0.0
14	2.907238733723097E-4	0.0	0.0	22.247353685942628	0.0
15	2.907238733723097E-4	0.0	0.0	22.72210577115961	0.0
16	2.907238733723097E-4	0.0	0.0	23.64224683038297	0.0
17	2.907238733723097E-4	0.0	0.0	24.87433460573482	0.0
18	2.907238733723097E-4	0.0	0.0	26.473606633155896	0.0
19	2.907238733723097E-4	0.0	0.0	27.163494384668386	0.0
20	2.907238733723097E-4	0.0	0.0	27.881000904151247	0.0
21	2.907238733723097E-4	0.0	0.0	28.633394288438783	0.0
22	2.907238733723097E-4	0.0	0.0	29.48783175228	0.0
23	2.907238733723097E-4	0.0	0.0	30.27191403876512	0.0
24	2.907238733723097E-4	0.0	0.0	30.85714119586358	0.0
25	2.907238733723097E-4	0.0	0.0	31.35311612383674	0.0
26	2.907238733723097E-4	0.0	0.0	31.80868043341115	0.0
27	2.907238733723097E-4	0.0	0.0	32.32820399512747	0.0
28	2.907238733723097E-4	0.0	0.0	32.84220380324971	0.0
29	2.907238733723097E-4	0.0	0.0	33.3620180888394	0.0
30	2.907238733723097E-4	0.0	0.0	34.06004610880632	0.0
31	2.907238733723097E-4	0.0	0.0	34.61620087856755	0.0
32	2.907238733723097E-4	0.0	0.0	35.1153737691478	0.0
33	2.907238733723097E-4	0.0	0.0	35.621814756562365	0.0
34	2.907238733723097E-4	0.0	0.0	36.063715044088276	0.0
35	2.907238733723097E-4	0.0	0.0	36.57044675537621	0.0
36	2.907238733723097E-4	0.0	0.0	37.07630629504403	0.0
37	2.907238733723097E-4	0.0	0.0	37.58274728245859	0.0
38	2.907238733723097E-4	0.0	0.0	38.04848692760103	0.0
39	2.907238733723097E-4	0.0	0.0	38.50812137140266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	30	2.1610504E-6	45.000004	36
ACCGGGT	60	0.0	45.000004	4
CGAACAC	35	1.2086093E-7	45.000004	24
ATTAGCG	30	2.1610504E-6	45.000004	1
AATCACG	30	2.1610504E-6	45.000004	1
CATGCGG	35	1.2086093E-7	45.000004	2
CTATCCG	30	2.1610504E-6	45.000004	1
TTTCGAA	35	1.2086093E-7	45.000004	21
AGCTTAG	30	2.1610504E-6	45.000004	1
ATAGCGG	35	1.2086093E-7	45.000004	2
TCCTCGG	35	1.2086093E-7	45.000004	2
TTCCGCG	35	1.2086093E-7	45.000004	40
ACGGGTC	30	2.1610504E-6	45.000004	5
TCTAGCG	35	1.2086093E-7	45.000004	1
TCAGGTA	30	2.1610504E-6	45.000004	12
CATTAGA	30	2.1610504E-6	45.000004	25
GGCCATA	30	2.1610504E-6	45.000004	14
CCACTCA	30	2.1610504E-6	45.000004	43
TACGAAT	35	1.2086093E-7	45.000004	11
TAACACG	30	2.1610504E-6	45.000004	1
>>END_MODULE