##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	331112
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.080069583705814	33.0	31.0	34.0	30.0	34.0
2	32.27665563313924	34.0	31.0	34.0	30.0	34.0
3	32.420981420184106	34.0	31.0	34.0	30.0	34.0
4	35.99570236053058	37.0	35.0	37.0	35.0	37.0
5	35.810773997922155	37.0	35.0	37.0	35.0	37.0
6	35.82581120587596	37.0	35.0	37.0	35.0	37.0
7	35.90407173403561	37.0	35.0	37.0	35.0	37.0
8	36.07471792022035	37.0	35.0	37.0	35.0	37.0
9	37.68273273091884	39.0	38.0	39.0	35.0	39.0
10	37.22940878011066	39.0	37.0	39.0	34.0	39.0
11	37.16687102853415	39.0	37.0	39.0	34.0	39.0
12	37.20100147382155	39.0	37.0	39.0	34.0	39.0
13	37.20172630409046	39.0	37.0	39.0	34.0	39.0
14	38.46418432433738	40.0	38.0	41.0	34.0	41.0
15	38.44659510981179	40.0	38.0	41.0	34.0	41.0
16	38.380985889970766	40.0	38.0	41.0	34.0	41.0
17	38.37493053709923	40.0	38.0	41.0	34.0	41.0
18	38.31774142888207	40.0	38.0	41.0	34.0	41.0
19	38.34480477904757	40.0	38.0	41.0	34.0	41.0
20	38.2698180676025	40.0	38.0	41.0	34.0	41.0
21	38.23894633839909	40.0	38.0	41.0	34.0	41.0
22	38.22464604121868	40.0	37.0	41.0	34.0	41.0
23	38.12708690714924	40.0	37.0	41.0	34.0	41.0
24	38.03029790524052	40.0	37.0	41.0	33.0	41.0
25	38.05137234530914	40.0	37.0	41.0	33.0	41.0
26	38.00027181135084	40.0	37.0	41.0	33.0	41.0
27	37.94873335910508	40.0	37.0	41.0	33.0	41.0
28	37.92932904878108	40.0	37.0	41.0	33.0	41.0
29	37.833865882239245	40.0	37.0	41.0	33.0	41.0
30	37.79918879412404	40.0	37.0	41.0	33.0	41.0
31	37.72755140254657	40.0	37.0	41.0	33.0	41.0
32	37.68371125178187	40.0	37.0	41.0	33.0	41.0
33	37.60691548479065	40.0	37.0	41.0	33.0	41.0
34	37.55476394694242	40.0	37.0	41.0	33.0	41.0
35	37.487378892942566	40.0	36.0	41.0	32.0	41.0
36	37.41118413104931	40.0	36.0	41.0	32.0	41.0
37	37.34654135156684	40.0	36.0	41.0	32.0	41.0
38	37.33371789605934	40.0	36.0	41.0	32.0	41.0
39	37.26209862523859	39.0	36.0	41.0	32.0	41.0
40	37.10217086665539	39.0	36.0	41.0	31.0	41.0
41	37.00616105728575	39.0	35.0	41.0	31.0	41.0
42	36.953372272826115	39.0	35.0	41.0	31.0	41.0
43	36.89802544154244	39.0	35.0	41.0	31.0	41.0
44	36.80222704100123	39.0	35.0	41.0	31.0	41.0
45	36.59803027374423	39.0	35.0	41.0	30.0	41.0
46	36.48411111648022	39.0	35.0	40.0	30.0	41.0
47	36.37332685012926	39.0	35.0	40.0	30.0	41.0
48	36.26535130107033	39.0	35.0	40.0	30.0	41.0
49	36.12444429679383	39.0	35.0	40.0	29.0	41.0
50	35.99132015752978	38.0	35.0	40.0	29.0	41.0
51	34.711339365531906	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	1.0
13	7.0
14	8.0
15	11.0
16	15.0
17	30.0
18	63.0
19	142.0
20	236.0
21	401.0
22	543.0
23	788.0
24	1054.0
25	1382.0
26	1712.0
27	2261.0
28	2972.0
29	3806.0
30	4927.0
31	6580.0
32	8529.0
33	11339.0
34	16981.0
35	22287.0
36	27712.0
37	41545.0
38	71303.0
39	104448.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.302797844838	33.85863393655319	19.211022252289254	26.627545966319556
2	21.42960690038416	30.97320544105922	20.62836744062432	26.968820217932297
3	20.76185701514895	31.264647611684264	21.24779530793206	26.725700065234726
4	20.565850829930657	30.949950469931625	19.942798811278358	28.54139988885936
5	18.928036434801516	33.71125178187441	18.1884075478992	29.172304235424875
6	21.18316460895407	34.440914252579184	19.327599120539276	25.04832201792747
7	87.82526758317427	3.9095532629442604	4.373142622435913	3.892036531445553
8	89.9055304549518	3.475561139433183	3.3725748387252654	3.246333566889753
9	81.31025151610332	8.942593442702167	6.202131000990602	3.5450240402039186
10	31.49055304549518	45.27924086109836	11.914095532629442	11.316110560777018
11	21.117023846915846	26.45358670178067	35.39134794269009	17.0380415086134
12	25.887313054193147	23.10547488463118	32.585952789388486	18.42125927178719
13	24.893087535335475	23.518628137911037	30.35921380076832	21.229070525985165
14	19.046727391335864	27.24244364444659	31.02394355988306	22.686885404334483
15	18.50944695450482	30.29428108917828	31.499009398632488	19.697262557684407
16	23.005206697431685	28.82951992075189	29.61535673729735	18.549916644519072
17	21.75245838266206	28.79025828118582	28.942472637657346	20.51481069849477
18	21.156587499093963	28.666433110246686	28.950928990794655	21.2260503998647
19	22.245040952910195	30.2245161757955	28.66431902196236	18.86612384933195
20	25.005134214404794	29.496363768150957	27.589758148300277	17.908743869143976
21	23.461245741622168	29.002573147454637	28.10468965183986	19.43149145908333
22	21.657324409867357	27.482845683635748	29.675457247094638	21.184372659402257
23	21.99437048491145	28.22036048225374	28.58337964193385	21.201889390900963
24	20.36924061948827	28.160561985068494	29.359854067505857	22.110343327937372
25	21.039406605619853	29.957839039358287	26.80814950832347	22.1946048466984
26	22.344101089661507	30.641595593031965	26.203218246393966	20.81108507091256
27	19.70179274686511	29.30518978472541	28.621735243663775	22.371282224745705
28	21.122460073932686	29.000761071782357	28.878143951291406	20.998634902993548
29	21.68692164584793	27.794522699267922	27.858851385633866	22.659704269250284
30	21.123366111768828	28.022844233975206	28.60693662567349	22.24685302858247
31	21.459204136364736	28.667641160694874	26.133453333011186	23.739701369929207
32	22.83456957162531	28.751600666843846	26.182077363550704	22.231752397980138
33	22.577254826161543	28.89203653144555	27.309490444320954	21.221218198071952
34	19.73531614680229	27.819891758679844	29.267438208219577	23.17735388629829
35	20.566152842542703	28.383447292758945	28.197105511126146	22.853294353572203
36	20.071456184010245	29.90891299620672	28.041569015922107	21.97806180386093
37	20.565850829930657	30.440455193408877	26.68432433738433	22.309369639276134
38	21.919471357123875	28.195293435453866	27.949455169247866	21.935780038174393
39	22.375812413926404	26.57288168353911	27.320060885742585	23.731245016791902
40	22.594469545048202	27.185665273381815	29.003479185290775	21.216385996279204
41	19.752228853076907	27.91955592065525	30.999178525695232	21.329036700572615
42	21.34957355819179	28.79599652081471	28.307944139747278	21.546485781246226
43	20.501522143564717	28.273816714586005	27.92318007199981	23.301481069849476
44	21.916753243615453	27.1007997293967	27.21556452197444	23.76688250501341
45	22.422322356181596	26.612445335717222	27.63808016622774	23.327152141873444
46	19.28682741791297	27.824421947860543	29.32602865495663	23.562721979269856
47	20.749172485442994	28.430259247626182	27.86307956220252	22.95748870472831
48	21.186486747686583	27.016538210635677	29.300055570320616	22.496919471357124
49	20.24662349899732	27.466537002585227	28.533245548334097	23.753593950083353
50	19.805685085409166	26.816605861460772	27.019558336756145	26.358150716373917
51	19.810517287201915	26.23432554543478	29.682705549783762	24.27245161757955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	829.0
1	670.5
2	512.0
3	363.5
4	215.0
5	214.5
6	214.0
7	250.5
8	287.0
9	336.5
10	386.0
11	495.5
12	605.0
13	598.0
14	591.0
15	700.0
16	809.0
17	811.0
18	813.0
19	1020.5
20	1228.0
21	1346.0
22	1464.0
23	1817.5
24	2171.0
25	2434.0
26	3469.5
27	4242.0
28	5167.5
29	6093.0
30	7286.0
31	8479.0
32	9489.0
33	10499.0
34	11059.5
35	11620.0
36	12898.0
37	14176.0
38	15069.5
39	15963.0
40	17257.5
41	18552.0
42	19549.0
43	20546.0
44	21960.0
45	23374.0
46	24989.5
47	26605.0
48	28210.5
49	29816.0
50	28602.0
51	27388.0
52	25600.0
53	23812.0
54	20717.0
55	17622.0
56	15854.5
57	14087.0
58	13227.0
59	12367.0
60	11853.5
61	11340.0
62	10118.5
63	8897.0
64	7069.5
65	5242.0
66	3985.0
67	2728.0
68	2361.5
69	1995.0
70	1628.0
71	1261.0
72	992.0
73	723.0
74	513.5
75	282.0
76	260.0
77	185.5
78	111.0
79	88.5
80	66.0
81	80.0
82	94.0
83	56.0
84	18.0
85	11.5
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	331112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.286525408292327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89940238999641	22.494824797156568
2	9.393178345296182	5.877598259294606
3	3.4026475692432196	3.1937105889177633
4	1.9773268361182788	2.4745474519485122
5	1.5026151700623254	2.3505803848520217
6	1.1541334772745266	2.166529581678615
7	0.9903944616362835	2.169020104175091
8	0.9055315859905868	2.2664749578484593
9	0.7268213372876314	2.046574281270464
>10	7.819319589879081	49.54883731578143
>50	0.20766323729614605	4.003501574999385
>100	0.018969238022244112	0.9161480938966371
>500	0.0019967618970783275	0.491652608180466
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGCT	953	0.28781801928048517	Illumina Paired End PCR Primer 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.18754983208098772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3581869578873614	0.0
2	0.0	0.0	0.0	0.5424146512358357	0.0
3	0.0	0.0	0.0	0.7804005895286187	0.0
4	0.0	0.0	0.0	1.8033173065307206	0.0
5	0.0	0.0	0.0	1.9670081422600207	0.0
6	0.0	0.0	0.0	2.9560994467128947	0.0
7	0.0	0.0	0.0	3.6905941192104184	0.0
8	0.0	0.0	0.0	4.245693300152214	0.0
9	0.0	0.0	0.0	5.12001981202735	0.0
10	0.0	0.0	0.0	6.391492908743869	0.0
11	0.0	0.0	0.0	7.580214549759598	0.0
12	0.0	0.0	0.0	8.200850467515524	0.0
13	0.0	0.0	0.0	8.449406847229941	0.0
14	0.0	0.0	0.0	8.726956437700839	0.0
15	0.0	0.0	0.0	8.977928918311628	0.0
16	0.0	0.0	0.0	9.42792771026118	0.0
17	0.0	0.0	0.0	10.050677716301433	0.0
18	0.0	0.0	0.0	10.680676025030806	0.0
19	0.0	0.0	0.0	11.083560849501076	0.0
20	0.0	0.0	0.0	11.49882819106526	0.0
21	0.0	0.0	0.0	11.980236294667666	0.0
22	0.0	0.0	0.0	12.49728188649158	0.0
23	0.0	0.0	0.0	12.97566986397352	0.0
24	0.0	0.0	0.0	13.30848776244896	0.0
25	0.0	0.0	0.0	13.613822513228152	0.0
26	0.0	0.0	0.0	13.916741163110972	0.0
27	0.0	0.0	0.0	14.240800695837057	0.0
28	0.0	0.0	0.0	14.578450796105246	0.0
29	0.0	0.0	0.0	14.949926308922661	0.0
30	0.0	0.0	0.0	15.445831017903307	0.0
31	0.0	0.0	0.0	15.80492401362681	0.0
32	0.0	0.0	0.0	16.168245185918963	0.0
33	0.0	0.0	0.0	16.511029500591945	0.0
34	0.0	0.0	0.0	16.851397714368552	0.0
35	0.0	0.0	0.0	17.20988668486796	0.0
36	0.0	0.0	0.0	17.579550122013096	0.0
37	0.0	0.0	0.0	17.93834110512455	0.0
38	0.0	0.0	0.0	18.30770252965764	0.0
39	0.0	0.0	0.0	18.67343980284617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	25	3.8847695E-5	45.000004	9
ACGCACG	30	2.160803E-6	45.000004	1
ACGGGCG	25	3.8847695E-5	45.000004	5
AGAACCG	30	2.160803E-6	45.000004	1
CTACGCG	25	3.8847695E-5	45.000004	1
GGACGTA	25	3.8847695E-5	45.000004	8
TTAACGG	25	3.8847695E-5	45.000004	2
ACTCGCT	25	3.8847695E-5	45.000004	18
CCGGTTC	30	2.160803E-6	45.000004	28
TATCGTG	25	3.8847695E-5	45.000004	1
TATCGGG	25	3.8847695E-5	45.000004	3
TTAGGCG	20	7.026005E-4	45.0	1
CGAACGG	40	6.7902874E-9	45.0	2
CTCCGCG	20	7.026005E-4	45.0	1
TTTACCG	20	7.026005E-4	45.0	1
CCGATAC	20	7.026005E-4	45.0	24
CGTGACG	20	7.026005E-4	45.0	1
AGCTTCG	20	7.026005E-4	45.0	1
TGCGGGT	20	7.026005E-4	45.0	4
CGACACG	20	7.026005E-4	45.0	1
>>END_MODULE