SRR3554071_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554071_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195004
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	2997	1.536891550942545	Illumina Single End Adapter 1 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1010	0.5179380935775677	No Hit
AAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	967	0.4958872638509979	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCC	905	0.4640930442452462	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	837	0.4292219646776476	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGT	824	0.42255543476031265	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	770	0.39486369510369024	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	574	0.2943529363500236	No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	447	0.22922606715759675	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	418	0.21435457734200325	No Hit
TACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	372	0.19076531763451007	No Hit
AACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	355	0.1820475477426104	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	337	0.1728169678570696	No Hit
AGCCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	271	0.1389715082767533	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	262	0.1343562183339829	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	258	0.1323049783594183	No Hit
CACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	239	0.12256158848023631	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.12204877848659515	No Hit
AACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	227	0.11640786855654243	No Hit
AATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	217	0.11127976862013086	No Hit
GACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	212	0.10871571865192509	No Hit
AAGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	211	0.10820290865828394	No Hit
GTTTCTGGGGTTCTGCTTGTTCTGTGGTTATGTCTAAGATTATAGTACACG	206	0.10563885869007816	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	202	0.10358761871551354	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	196	0.1005107587536666	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAGG	30	2.1562064E-6	45.000004	2
GTACACG	30	2.1562064E-6	45.000004	45
CCGATTG	30	2.1562064E-6	45.000004	26
TAGGTTC	30	2.1562064E-6	45.000004	14
AAGGTTG	30	2.1562064E-6	45.000004	10
GTAGGTT	30	2.1562064E-6	45.000004	13
TTGAGCG	30	2.1562064E-6	45.000004	1
CGAGGGC	35	1.2050077E-7	45.000004	4
GATATCT	30	2.1562064E-6	45.000004	9
TAGGGTA	30	2.1562064E-6	45.000004	5
ATTGGGC	35	1.2050077E-7	45.000004	4
CGTAAGG	35	1.2050077E-7	45.000004	2
TTCGGGA	30	2.1562064E-6	45.000004	4
ACGTAGG	30	2.1562064E-6	45.000004	2
ACACTAG	60	0.0	45.000004	1
TGGGACA	65	0.0	45.000004	6
CCAGTGG	35	1.2050077E-7	45.000004	42
GACCTAC	35	1.2050077E-7	45.000004	9
TCGAAGG	35	1.2050077E-7	45.000004	2
GTTATGT	30	2.1562064E-6	45.000004	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content