FastQCFastQC Report
Sat 18 Jun 2016
SRR3554070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564326
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT18510.3280018996112176No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC6710.11890290364080336No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT6420.11376403001102199No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT5680.10065104212813163No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA207.030231E-445.044
CTCGTAA253.8882732E-545.033
CGAAATA207.030231E-445.022
CGTGATT207.030231E-445.017
GACAACG207.030231E-445.01
GAATCGG253.8882732E-545.026
ATTCGCC207.030231E-445.034
CTGCGTT351.2104647E-745.045
CGTTAAG351.2104647E-745.01
CTACGTC207.030231E-445.030
TTGCGTA207.030231E-445.036
AACGACT207.030231E-445.026
GTTCACG207.030231E-445.01
GATTGCC207.030231E-445.017
TTCGCTA207.030231E-445.013
CGTCCAA253.8882732E-545.021
TCGACAA207.030231E-445.010
CGTCATT207.030231E-445.022
TATAACG207.030231E-445.01
GCGTACT207.030231E-445.038