##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564326
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071653618653045	33.0	31.0	34.0	30.0	34.0
2	32.2644552971155	34.0	31.0	34.0	30.0	34.0
3	32.38401030609967	34.0	31.0	34.0	30.0	34.0
4	35.98458160708527	37.0	35.0	37.0	35.0	37.0
5	35.79703221187753	37.0	35.0	37.0	35.0	37.0
6	35.79076101402381	37.0	35.0	37.0	35.0	37.0
7	35.739570744569626	37.0	35.0	37.0	35.0	37.0
8	35.91899540336614	37.0	35.0	37.0	35.0	37.0
9	37.54791379450885	39.0	37.0	39.0	35.0	39.0
10	37.193508716592895	39.0	37.0	39.0	34.0	39.0
11	37.15809656120753	39.0	37.0	39.0	34.0	39.0
12	37.03965438416802	39.0	37.0	39.0	33.0	39.0
13	36.97966423662918	39.0	37.0	39.0	33.0	39.0
14	38.21378954717663	40.0	37.0	41.0	33.0	41.0
15	38.20909013584347	40.0	37.0	41.0	33.0	41.0
16	38.132341944195375	40.0	37.0	41.0	33.0	41.0
17	38.14683356783136	40.0	37.0	41.0	33.0	41.0
18	38.10010525830814	40.0	37.0	41.0	33.0	41.0
19	38.099695920443146	40.0	37.0	41.0	33.0	41.0
20	38.03501876574887	40.0	37.0	41.0	33.0	41.0
21	37.98937670778947	40.0	37.0	41.0	33.0	41.0
22	37.98567671877603	40.0	37.0	41.0	33.0	41.0
23	37.859671537373785	40.0	37.0	41.0	33.0	41.0
24	37.747231210328785	40.0	36.0	41.0	33.0	41.0
25	37.74877818849389	40.0	36.0	41.0	33.0	41.0
26	37.68372359239163	40.0	36.0	41.0	33.0	41.0
27	37.62674588801509	40.0	36.0	41.0	33.0	41.0
28	37.57969684189635	40.0	36.0	41.0	33.0	41.0
29	37.43113909336093	39.0	36.0	41.0	32.0	41.0
30	37.42364342596301	39.0	36.0	41.0	32.0	41.0
31	37.33908591842304	39.0	36.0	41.0	32.0	41.0
32	37.30305001010054	39.0	36.0	41.0	32.0	41.0
33	37.235654568458656	39.0	36.0	41.0	32.0	41.0
34	37.13974901032382	39.0	35.0	41.0	31.0	41.0
35	37.05491683884847	39.0	35.0	41.0	31.0	41.0
36	36.95642057959407	39.0	35.0	41.0	31.0	41.0
37	36.869793346399064	39.0	35.0	41.0	31.0	41.0
38	36.82765281060947	39.0	35.0	41.0	31.0	41.0
39	36.72615119629434	39.0	35.0	41.0	31.0	41.0
40	36.60077153985462	39.0	35.0	40.0	31.0	41.0
41	36.49063307379068	39.0	35.0	40.0	30.0	41.0
42	36.38993064292625	38.0	35.0	40.0	30.0	41.0
43	36.29147868430659	38.0	35.0	40.0	30.0	41.0
44	36.15679589457158	38.0	35.0	40.0	30.0	41.0
45	35.96415369839419	38.0	35.0	40.0	30.0	41.0
46	35.8314750693748	38.0	35.0	40.0	29.0	41.0
47	35.72097511013138	38.0	34.0	40.0	29.0	41.0
48	35.64790209914128	38.0	34.0	40.0	29.0	41.0
49	35.49209499473709	38.0	34.0	40.0	28.0	41.0
50	35.30250777033133	37.0	34.0	40.0	28.0	41.0
51	34.05461736655763	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	2.0
12	7.0
13	6.0
14	20.0
15	30.0
16	47.0
17	114.0
18	176.0
19	311.0
20	529.0
21	839.0
22	1164.0
23	1579.0
24	2092.0
25	2653.0
26	3481.0
27	4330.0
28	5573.0
29	7341.0
30	9187.0
31	12526.0
32	16530.0
33	22672.0
34	36276.0
35	43798.0
36	52099.0
37	76263.0
38	117834.0
39	146794.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.90577786598526	31.957060280759702	19.840659476968987	26.296502376286046
2	23.12705776448365	30.0345899356046	20.391404968050384	26.44694733186137
3	21.28326534662589	30.76360118087772	20.92035454683995	27.032778925656448
4	21.190411216211906	30.53394669038818	19.6554828237579	28.620159269642016
5	20.233517505838826	32.41264800842066	17.408731832309694	29.945102653430816
6	22.147304926584987	35.84399797280295	18.77017893912384	23.238518161488216
7	84.29507057977126	5.528010405333088	5.459610225295308	4.717308789600337
8	85.72970233517506	4.399407434709725	4.809454109858486	5.061436120256731
9	77.95051087491981	8.797574451646744	7.920067478726835	5.331847194706605
10	38.179527436269105	31.70206582719917	14.104967695977146	16.013439040554573
11	28.42523647678824	27.376551851234925	24.27940587532738	19.918805796649455
12	28.146142477929427	22.4373854828592	28.565403685104002	20.851068354107376
13	26.560711361872393	23.862093896081344	26.320956326662248	23.256238415384015
14	22.309622452270496	24.73357598267668	28.918213940169334	24.03858762488349
15	22.148545344357697	26.558584931404898	28.70663410865351	22.5862356155839
16	24.93080240853691	25.730517466854263	27.093736599057994	22.244943525550834
17	24.653126029989757	25.94581855168821	26.138437711535527	23.262617706786504
18	24.746688970559568	25.52017805311115	26.278782122390247	23.454350853939037
19	24.268242115373027	28.624057725499092	25.38904817428224	21.71865198484564
20	25.085854630125144	26.674120986805498	25.6257907663301	22.61423361673926
21	25.989055971193952	26.659590378610943	25.342621109075253	22.008732541119848
22	24.733930387754597	25.354848084263352	26.053380492835704	23.85784103514635
23	25.766666784801696	25.871570687864814	24.18371650429007	24.178046023043418
24	23.288489277474365	26.08846659554938	26.185396384359393	24.437647742616857
25	22.95889255501253	27.154871474998494	24.692819398716345	25.193416571272632
26	24.579586976322197	26.47352771270507	24.788331567214694	24.15855374375804
27	23.534978009164913	25.529924192753832	25.4769406336054	25.458157164475853
28	23.6923338637596	26.409025988524366	26.662071214156356	23.236568933559678
29	24.81526635313631	26.47317330762715	25.31976198154967	23.39179835768687
30	24.830682974025652	25.524608116585096	25.61533581653158	24.029373092857675
31	25.805828545911407	26.87170181774364	23.96487136867697	23.357598267667978
32	25.64971310908943	26.299514819448333	23.892572732782117	24.158199338680124
33	25.03145345066504	24.84840322792145	26.517651144905603	23.602492176507905
34	23.713775370973515	26.809149321491475	26.13188121759409	23.34519408994092
35	24.712843285618597	26.51463870174332	27.05457483794828	21.717943174689808
36	25.115801859209043	28.027771181905493	25.072741642242253	21.78368531664322
37	26.496032435152735	27.29893713917133	23.83888036347785	22.36615006219809
38	26.121603470334527	27.008502177819203	25.057502223891863	21.812392127954407
39	24.695654639339672	24.591105141354465	26.392900557479187	24.320339661826676
40	25.0139103993082	24.54609569645914	26.095377494568744	24.344616409663917
41	23.631376190358054	24.287025584502576	27.654759837398952	24.426838387740418
42	25.496432912890775	24.97634346104911	25.770033633041894	23.757189993018223
43	23.763392081881747	24.858326570103095	26.083504924458556	25.2947764235566
44	24.89128624234928	24.07278771490238	25.988169958499164	25.047756084249173
45	25.343861526847956	23.769771373284236	26.71363715299313	24.17272994687468
46	22.316887756367773	25.259158713226043	28.591098053252907	23.832855477153277
47	23.54153450310636	24.542020038063107	27.506086907213206	24.41035855161733
48	24.265052469671787	24.047802156909302	27.756296892221872	23.93084848119704
49	24.71603293131984	24.119923590265202	26.30500809815603	24.859035380258927
50	23.124754131477196	24.051346207688464	26.816591828127713	26.007307832706626
51	23.825590173056	22.81677611876823	28.581529116149174	24.77610459202659
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	754.0
1	643.5
2	533.0
3	392.5
4	252.0
5	246.0
6	240.0
7	275.0
8	310.0
9	340.0
10	370.0
11	454.5
12	539.0
13	649.5
14	760.0
15	717.5
16	675.0
17	730.0
18	785.0
19	921.0
20	1057.0
21	1114.0
22	1171.0
23	1736.0
24	2301.0
25	2664.5
26	3785.5
27	4543.0
28	5265.5
29	5988.0
30	7179.0
31	8370.0
32	9643.5
33	10917.0
34	11530.5
35	12144.0
36	13829.0
37	15514.0
38	17680.5
39	19847.0
40	20991.5
41	22136.0
42	24102.5
43	26069.0
44	29090.0
45	32111.0
46	36623.0
47	41135.0
48	44822.0
49	48509.0
50	48478.0
51	48447.0
52	44158.0
53	39869.0
54	37422.0
55	34975.0
56	33842.5
57	32710.0
58	32005.0
59	31300.0
60	29759.0
61	28218.0
62	25392.0
63	22566.0
64	20328.5
65	18091.0
66	16783.5
67	15476.0
68	12989.0
69	10502.0
70	8988.0
71	7474.0
72	6764.0
73	6054.0
74	4912.0
75	2855.0
76	1940.0
77	1644.5
78	1349.0
79	1013.5
80	678.0
81	521.5
82	365.0
83	277.0
84	189.0
85	186.0
86	183.0
87	113.5
88	44.0
89	35.0
90	26.0
91	21.0
92	16.0
93	19.5
94	23.0
95	13.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	564326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.065047509974054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3780956137746	20.401117389981763
2	9.793173516930679	5.301054130182971
3	3.3363015954983024	2.708914835692914
4	1.6255071962093037	1.7597771797283808
5	0.9343380713596521	1.2643952145863255
6	0.7065882058099187	1.1474306016139664
7	0.489399195455975	0.9271928733451328
8	0.3965946330584667	0.8587082068742502
9	0.3398169220296717	0.8277445025483592
>10	5.609366453379037	38.33081993570859
>50	1.3198651678588948	23.062965150606416
>100	0.06766854768017232	2.649560492727236
>500	0.002627904764278536	0.43119147673736985
>1k	6.56976191069634E-4	0.3291280096663388
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	1851	0.3280018996112176	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	671	0.11890290364080336	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	642	0.11376403001102199	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT	568	0.10065104212813163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3843523069998547	0.0
2	0.0	0.0	0.0	0.5647444916590765	0.0
3	0.0	0.0	0.0	0.7734890825515748	0.0
4	0.0	0.0	0.0	1.7546595407619001	0.0
5	0.0	0.0	0.0	1.9155594461357441	0.0
6	0.0	0.0	0.0	2.883971321541095	0.0
7	0.0	0.0	0.0	3.5894146291328064	0.0
8	0.0	0.0	0.0	4.230710617621729	0.0
9	0.0	0.0	0.0	5.099357463593738	0.0
10	0.0	0.0	0.0	6.452653253615818	0.0
11	0.0	0.0	0.0	7.699981925341026	0.0
12	0.0	0.0	0.0	8.270396898246759	0.0
13	0.0	0.0	0.0	8.535137491449978	0.0
14	0.0	0.0	0.0	8.839748655918742	0.0
15	0.0	0.0	0.0	9.098287160258431	0.0
16	0.0	0.0	0.0	9.55245726760773	0.0
17	0.0	0.0	0.0	10.108518834857867	0.0
18	0.0	0.0	0.0	10.77515478641778	0.0
19	0.0	0.0	0.0	11.115029256139183	0.0
20	0.0	0.0	0.0	11.50788728500902	0.0
21	0.0	0.0	0.0	11.92431325156027	0.0
22	0.0	0.0	0.0	12.398684448350776	0.0
23	0.0	0.0	0.0	12.844348833830091	0.0
24	0.0	0.0	0.0	13.193260633038351	0.0
25	0.0	0.0	0.0	13.498935012740862	0.0
26	0.0	0.0	0.0	13.801065341664215	0.0
27	0.0	0.0	0.0	14.1494455332556	0.0
28	0.0	0.0	0.0	14.462208014516431	0.0
29	0.0	0.0	0.0	14.76823679929686	0.0
30	0.0	0.0	0.0	15.19157366486747	0.0
31	0.0	0.0	0.0	15.519752767017646	0.0
32	0.0	0.0	0.0	15.84810907170678	0.0
33	0.0	0.0	0.0	16.172566920538838	0.0
34	0.0	0.0	0.0	16.47452004692323	0.0
35	0.0	0.0	0.0	16.815457731878382	0.0
36	0.0	0.0	0.0	17.125562175054846	0.0
37	0.0	0.0	0.0	17.472524746334564	0.0
38	3.5440507791595637E-4	0.0	0.0	17.825689406477817	0.0
39	3.5440507791595637E-4	0.0	0.0	18.173892395530242	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAA	20	7.030231E-4	45.0	44
CTCGTAA	25	3.8882732E-5	45.0	33
CGAAATA	20	7.030231E-4	45.0	22
CGTGATT	20	7.030231E-4	45.0	17
GACAACG	20	7.030231E-4	45.0	1
GAATCGG	25	3.8882732E-5	45.0	26
ATTCGCC	20	7.030231E-4	45.0	34
CTGCGTT	35	1.2104647E-7	45.0	45
CGTTAAG	35	1.2104647E-7	45.0	1
CTACGTC	20	7.030231E-4	45.0	30
TTGCGTA	20	7.030231E-4	45.0	36
AACGACT	20	7.030231E-4	45.0	26
GTTCACG	20	7.030231E-4	45.0	1
GATTGCC	20	7.030231E-4	45.0	17
TTCGCTA	20	7.030231E-4	45.0	13
CGTCCAA	25	3.8882732E-5	45.0	21
TCGACAA	20	7.030231E-4	45.0	10
CGTCATT	20	7.030231E-4	45.0	22
TATAACG	20	7.030231E-4	45.0	1
GCGTACT	20	7.030231E-4	45.0	38
>>END_MODULE