##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030208
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0089273234143	33.0	31.0	34.0	30.0	34.0
2	32.21052544728831	34.0	31.0	34.0	30.0	34.0
3	32.33081863080077	34.0	31.0	34.0	30.0	34.0
4	35.92485303938622	37.0	35.0	37.0	35.0	37.0
5	35.71625341678573	37.0	35.0	37.0	33.0	37.0
6	35.7147508076039	37.0	35.0	37.0	33.0	37.0
7	35.5951536000497	37.0	35.0	37.0	35.0	37.0
8	35.82445875007765	37.0	35.0	37.0	35.0	37.0
9	37.49385852177424	39.0	37.0	39.0	35.0	39.0
10	37.113640158104	39.0	37.0	39.0	33.0	39.0
11	37.07767848822762	39.0	37.0	39.0	33.0	39.0
12	36.90888830216811	39.0	37.0	39.0	33.0	39.0
13	36.907401223830526	39.0	37.0	39.0	33.0	39.0
14	38.09265701683543	40.0	37.0	41.0	33.0	41.0
15	38.108583897620676	40.0	37.0	41.0	33.0	41.0
16	38.00864485618438	40.0	37.0	41.0	33.0	41.0
17	38.00701314685966	40.0	37.0	41.0	33.0	41.0
18	37.94154190221781	40.0	37.0	41.0	33.0	41.0
19	37.93730392309126	40.0	37.0	41.0	33.0	41.0
20	37.90457363949805	40.0	37.0	41.0	33.0	41.0
21	37.88548040783997	40.0	37.0	41.0	33.0	41.0
22	37.86143380754178	40.0	37.0	41.0	33.0	41.0
23	37.766551997266575	40.0	36.0	41.0	33.0	41.0
24	37.64448732683109	40.0	36.0	41.0	33.0	41.0
25	37.599754612660746	40.0	36.0	41.0	32.0	41.0
26	37.54797866061999	40.0	36.0	41.0	32.0	41.0
27	37.483319873268314	39.0	36.0	41.0	32.0	41.0
28	37.38767899298006	39.0	36.0	41.0	32.0	41.0
29	37.26949315089768	39.0	36.0	41.0	32.0	41.0
30	37.286050001553086	39.0	36.0	41.0	32.0	41.0
31	37.199899437783436	39.0	36.0	41.0	32.0	41.0
32	37.10608634372865	39.0	35.0	41.0	31.0	41.0
33	37.05312228210226	39.0	35.0	41.0	31.0	41.0
34	36.92854355625272	39.0	35.0	41.0	31.0	41.0
35	36.867224871093995	39.0	35.0	41.0	31.0	41.0
36	36.802656356774555	39.0	35.0	41.0	31.0	41.0
37	36.75074742187985	39.0	35.0	41.0	31.0	41.0
38	36.703108498477974	39.0	35.0	41.0	31.0	41.0
39	36.57216697987202	39.0	35.0	40.0	30.0	41.0
40	36.46891210318693	39.0	35.0	40.0	30.0	41.0
41	36.39392724576008	38.0	35.0	40.0	30.0	41.0
42	36.31324548052432	38.0	35.0	40.0	30.0	41.0
43	36.2629808737653	38.0	35.0	40.0	30.0	41.0
44	36.18633518668075	38.0	35.0	40.0	30.0	41.0
45	36.03174795769398	38.0	35.0	40.0	30.0	41.0
46	35.820869183698825	38.0	35.0	40.0	29.0	41.0
47	35.75966115580543	38.0	34.0	40.0	29.0	41.0
48	35.70511197738709	38.0	34.0	40.0	29.0	41.0
49	35.53076271976145	38.0	34.0	40.0	28.0	41.0
50	35.39131709324719	37.0	34.0	40.0	28.0	41.0
51	34.08386558830838	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	9.0
11	9.0
12	14.0
13	14.0
14	33.0
15	44.0
16	76.0
17	168.0
18	317.0
19	614.0
20	1016.0
21	1711.0
22	2534.0
23	3323.0
24	4313.0
25	5206.0
26	6642.0
27	8201.0
28	10598.0
29	14083.0
30	18210.0
31	24061.0
32	31930.0
33	43618.0
34	67829.0
35	81311.0
36	95289.0
37	136099.0
38	207840.0
39	265012.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.462469714853697	34.46847626886998	20.36918758153693	24.69986643473939
2	22.572917313785176	32.73736954090825	19.794934615145678	24.8947785301609
3	20.657770081381624	29.408236006709327	23.50554451139964	26.428449400509415
4	23.125524165993664	29.260304715164313	19.448402652668197	28.165768466173823
5	18.871431788532025	34.56253494439957	17.843775237621916	28.72225802944648
6	21.428682363173262	34.6794045474312	21.23425560663478	22.65765748276076
7	82.06061300242281	6.934716096166987	5.989470087593961	5.0152008138162385
8	83.27726051438155	4.376494843759707	4.907940920668447	7.438303721190284
9	77.98823150276449	7.459173293160217	8.523812666956577	6.028782537118717
10	39.951058427036095	29.296413928061128	14.454847953034728	16.29767969186805
11	28.8077747406349	26.860012735292287	23.590381748151827	20.74183077592098
12	29.309129806796296	21.87927098217059	28.615483475181712	20.1961157358514
13	26.93727868546934	23.39585792383674	25.959029632850843	23.707833757843076
14	21.568265825930297	24.267623625520283	30.150027955519658	24.014082593029755
15	20.985956234080884	27.742941231285336	27.482508386655898	23.788594147977886
16	22.911392650804498	25.499219575076097	28.609562340808846	22.979825433310555
17	22.336363142200412	25.897294526930487	27.721295117102567	24.04504721376654
18	23.134939740324285	26.162677828166743	26.248291607131762	24.454090824377214
19	24.107655929676337	27.97454494626328	26.453881157979747	21.463917966080636
20	25.956700006212337	27.304389016586946	25.4991225072995	21.239788469901224
21	25.340804963657824	26.053961918369883	25.90544822016525	22.699784897807046
22	24.23869742809219	25.733152916692553	26.06638659377524	23.96176306144002
23	25.642200410014286	25.626281294651175	24.08426259551469	24.64725569981984
24	23.09339473193763	27.759442753308072	25.117646145244453	24.029516369509846
25	23.47690951730136	25.912242964527554	24.60241038702864	26.008437131142447
26	25.68248353730509	27.56025967571597	23.65881453065789	23.098442256321054
27	22.42702444554886	26.192089364477855	25.45398599117848	25.926900198794804
28	24.150559886935454	28.441732155059952	24.62230928123253	22.78539867677207
29	23.594555662545815	28.539964744983536	24.81887152885631	23.046608063614336
30	24.566301174131826	25.337892930359697	24.426232372491768	25.66957352301671
31	26.639474746847235	27.667131297757347	21.996237652978817	23.6971563024166
32	24.853039386221035	25.804400664720134	24.97699493694477	24.36556501211406
33	25.80770096912468	26.498920606324162	24.609399266944152	23.083979157607008
34	24.885460023606885	27.085598248120768	24.99951466111698	23.02942706715537
35	24.927587438653166	26.786532428402808	24.96282304156054	23.323057091383486
36	24.88157731254271	28.589954649934768	24.574357799589986	21.954110237932532
37	25.564157917624403	26.635106696900046	23.876828756911227	23.92390662856433
38	26.274985245697955	25.527272162514752	26.443494905883085	21.754247685904204
39	25.01523964092688	24.066013853513077	27.207709511089025	23.71103699447102
40	25.82391128781761	25.325662390507546	26.263240044728832	22.587186276946014
41	24.89506973349071	24.144735820339193	28.2436168230105	22.716577623159594
42	25.910981083431693	25.419526930483944	24.33625054357955	24.333241442504814
43	23.65124324408275	24.419825899235885	26.61462539603653	25.314305460644842
44	24.19006647201342	23.105431136236565	25.571049729763313	27.133452661986706
45	26.369626327887186	24.230932005963844	25.827211592222156	23.57223007392682
46	21.440524631918993	25.073965645772507	28.943766695657576	24.541743026650927
47	23.911287817605764	25.969707088277318	25.70752702366901	24.41147807044791
48	23.87333431695347	23.482830651674224	29.05520050319935	23.588634528172953
49	24.179777287693362	24.739373019817357	26.122006429769524	24.958843262719764
50	22.37625799838479	23.844116916195564	26.489796235323354	27.289828850096292
51	22.627081133130396	23.372852860781514	28.895815214015037	25.104250792073056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	913.0
1	783.0
2	653.0
3	533.5
4	414.0
5	430.0
6	446.0
7	437.0
8	428.0
9	504.5
10	581.0
11	654.5
12	728.0
13	898.5
14	1069.0
15	1065.5
16	1062.0
17	1200.5
18	1339.0
19	1707.5
20	2076.0
21	2199.0
22	2322.0
23	3178.0
24	4034.0
25	4695.0
26	6703.5
27	8051.0
28	10351.0
29	12651.0
30	14116.5
31	15582.0
32	18314.5
33	21047.0
34	22207.0
35	23367.0
36	27617.5
37	31868.0
38	32620.0
39	33372.0
40	35743.0
41	38114.0
42	43304.0
43	48494.0
44	62533.0
45	76572.0
46	82094.5
47	87617.0
48	87044.0
49	86471.0
50	81334.0
51	76197.0
52	70562.0
53	64927.0
54	62499.5
55	60072.0
56	56849.5
57	53627.0
58	53213.5
59	52800.0
60	52065.0
61	51330.0
62	47451.5
63	43573.0
64	38572.0
65	33571.0
66	29004.5
67	24438.0
68	21791.5
69	19145.0
70	17315.0
71	15485.0
72	13318.0
73	11151.0
74	9309.5
75	6280.5
76	5093.0
77	3808.0
78	2523.0
79	2255.5
80	1988.0
81	1506.0
82	1024.0
83	731.0
84	438.0
85	408.0
86	378.0
87	293.5
88	209.0
89	157.0
90	105.0
91	66.0
92	27.0
93	17.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1030208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.07496722109414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73984502966246	18.740991642284424
2	9.381816650859003	4.704974899892094
3	3.172885717413945	2.386800160812973
4	1.5842184138362163	1.5889689919198753
5	1.0004361138408011	1.2542951380678447
6	0.6969523387466366	1.0485634229242087
7	0.552760357239509	0.9702313487230684
8	0.4808926121644918	0.9646693189512776
9	0.4213640222211715	0.9509120140809832
>10	6.702427232785982	41.4432733201835
>50	1.196067460708351	19.1954398525166
>100	0.06489384771869629	2.799582601888272
>500	0.0019429295724160552	0.38273564180067965
>1k	0.0023315154868992663	1.1543455062150336
>5k	0.0011657577434496331	2.414216139739165
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	9148	0.8879760203764677	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC	8384	0.8138162390507547	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGT	7245	0.7032560414984158	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	3931	0.38157342983164566	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	1981	0.19229126545319003	No Hit
AACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	1943	0.18860268994222526	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	1702	0.16520935578058024	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTAT	1196	0.1160930608187861	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGTC	1094	0.10619214760514382	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.706777660433622E-5	0.0	0.0	0.4285542337081444	0.0
2	9.706777660433622E-5	0.0	0.0	0.5810477107535565	0.0
3	9.706777660433622E-5	0.0	0.0	0.9276767410076412	0.0
4	9.706777660433622E-5	0.0	0.0	2.492700503199354	0.0
5	9.706777660433622E-5	0.0	0.0	2.7263426414859913	0.0
6	9.706777660433622E-5	0.0	0.0	3.7111923029135863	0.0
7	9.706777660433622E-5	0.0	0.0	4.466864943778344	0.0
8	9.706777660433622E-5	0.0	0.0	5.011706373858483	0.0
9	9.706777660433622E-5	0.0	0.0	6.45034789091135	0.0
10	9.706777660433622E-5	0.0	0.0	7.51566673914394	0.0
11	9.706777660433622E-5	0.0	0.0	9.351606665838355	0.0
12	9.706777660433622E-5	0.0	0.0	9.84888488538237	0.0
13	9.706777660433622E-5	0.0	0.0	10.120286388768093	0.0
14	9.706777660433622E-5	0.0	0.0	10.499821395291049	0.0
15	9.706777660433622E-5	0.0	0.0	10.748411971174754	0.0
16	9.706777660433622E-5	0.0	0.0	11.133285705410946	0.0
17	9.706777660433622E-5	0.0	0.0	11.580768155556935	0.0
18	9.706777660433622E-5	0.0	0.0	12.213650059017208	0.0
19	9.706777660433622E-5	0.0	0.0	12.513298285394795	0.0
20	9.706777660433622E-5	0.0	0.0	12.83041871156116	0.0
21	9.706777660433622E-5	0.0	0.0	13.207915294775423	0.0
22	9.706777660433622E-5	0.0	0.0	13.608611076598123	0.0
23	9.706777660433622E-5	0.0	0.0	14.003094520718147	0.0
24	9.706777660433622E-5	0.0	0.0	14.305557712617258	0.0
25	9.706777660433622E-5	0.0	0.0	14.584433434801516	0.0
26	9.706777660433622E-5	0.0	0.0	14.840207026153942	0.0
27	9.706777660433622E-5	0.0	0.0	15.104134310741133	0.0
28	9.706777660433622E-5	0.0	0.0	15.369517611977388	0.0
29	9.706777660433622E-5	0.0	0.0	15.655576349630365	0.0
30	9.706777660433622E-5	0.0	0.0	16.02928728955706	0.0
31	9.706777660433622E-5	0.0	0.0	16.325246940423682	0.0
32	9.706777660433622E-5	0.0	0.0	16.64062014661117	0.0
33	9.706777660433622E-5	0.0	0.0	16.93949183077592	0.0
34	9.706777660433622E-5	0.0	0.0	17.21797928185376	0.0
35	9.706777660433622E-5	0.0	0.0	17.511318102752064	0.0
36	9.706777660433622E-5	0.0	0.0	17.811742871342485	0.0
37	9.706777660433622E-5	0.0	0.0	18.126048331987327	0.0
38	9.706777660433622E-5	0.0	0.0	18.42967633720569	0.0
39	9.706777660433622E-5	0.0	0.0	18.736410511275395	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	20	7.032944E-4	45.000004	38
CGTACGT	20	7.032944E-4	45.000004	12
TATAGCG	20	7.032944E-4	45.000004	1
GTGCGAA	20	7.032944E-4	45.000004	16
CAATACG	20	7.032944E-4	45.000004	1
ATTCGCG	55	1.8189894E-12	45.0	1
ATATACG	35	1.2117562E-7	45.0	1
ACTACCG	55	1.8189894E-12	45.0	1
TAATCCG	90	0.0	45.0	1
TATCGCG	70	0.0	41.785713	1
CATACCG	60	3.6379788E-12	41.249996	1
TTACCGG	110	0.0	40.909092	2
TATCTCG	50	1.0804797E-9	40.5	1
TACGGCT	1020	0.0	40.14706	6
TGCGATG	45	1.9279469E-8	40.0	1
ATTACGG	130	0.0	39.807693	2
TAAACGG	160	0.0	39.375004	2
ATGTACG	80	0.0	39.375004	1
AATTGCG	40	3.4577533E-7	39.375004	1
TTACGCG	80	0.0	39.375004	1
>>END_MODULE