FastQCFastQC Report
Sat 18 Jun 2016
SRR3554067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039073
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT93570.9005142083376241No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC82550.7944581372049895No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGT73440.7067838352069585No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT40330.3881344236641699Illumina Single End Adapter 2 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG20090.19334541461475757No Hit
AATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT19480.18747479724716165No Hit
AATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT17140.16495472406654776No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTAT11750.11308156404795427No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGTC11040.10624855039058853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG453.8562575E-1045.0000041
ATTCGCT207.032972E-445.00000425
TAAATCG207.032972E-445.00000444
GCTCGAA207.032972E-445.00000421
TTCGATA207.032972E-445.00000443
TATCGAG207.032972E-445.0000041
TAATGCG207.032972E-445.0000041
ACGGTAC207.032972E-445.00000413
TAATCCG600.045.0000041
AGCGTAA253.890546E-545.011
CGGTTTA253.890546E-545.043
ATACGGT502.1827873E-1145.027
TAATCGC253.890546E-545.030
TCAACCG800.042.1875041
CATACCG700.041.7857131
TCGTTGA2000.041.62524
TCACGCG650.041.538461
ATATACG603.6379788E-1241.2500041
CATAACG603.6379788E-1241.2500041
TATCGCG501.0804797E-940.51