##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1039073
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.982474763563292	33.0	31.0	34.0	30.0	34.0
2	32.17060014070234	34.0	31.0	34.0	30.0	34.0
3	32.30477839381834	34.0	31.0	34.0	30.0	34.0
4	35.86877149151215	37.0	35.0	37.0	35.0	37.0
5	35.68690553984176	37.0	35.0	37.0	33.0	37.0
6	35.69251149822967	37.0	35.0	37.0	33.0	37.0
7	35.61218509190403	37.0	35.0	37.0	35.0	37.0
8	35.831215901096456	37.0	35.0	37.0	35.0	37.0
9	37.499668454478176	39.0	37.0	39.0	35.0	39.0
10	37.09217157986013	39.0	37.0	39.0	33.0	39.0
11	37.063620169131525	39.0	37.0	39.0	33.0	39.0
12	36.89537693694283	39.0	37.0	39.0	33.0	39.0
13	36.886332336611574	39.0	37.0	39.0	33.0	39.0
14	38.07416707007111	40.0	37.0	41.0	33.0	41.0
15	38.08919970011732	40.0	37.0	41.0	33.0	41.0
16	38.01237352909757	40.0	37.0	41.0	33.0	41.0
17	37.985443756117235	40.0	37.0	41.0	33.0	41.0
18	37.9077033086222	40.0	37.0	41.0	33.0	41.0
19	37.90051324594133	40.0	37.0	41.0	33.0	41.0
20	37.86606138356015	40.0	37.0	41.0	33.0	41.0
21	37.87038927967525	40.0	37.0	41.0	33.0	41.0
22	37.82371498441399	40.0	37.0	41.0	33.0	41.0
23	37.73998169522257	40.0	36.0	41.0	33.0	41.0
24	37.6321509653316	40.0	36.0	41.0	33.0	41.0
25	37.59165910383582	39.0	36.0	41.0	32.0	41.0
26	37.528753995147596	39.0	36.0	41.0	32.0	41.0
27	37.45533951897509	39.0	36.0	41.0	32.0	41.0
28	37.36399944950932	39.0	36.0	41.0	32.0	41.0
29	37.243496847670954	39.0	36.0	41.0	32.0	41.0
30	37.26638166904539	39.0	36.0	41.0	32.0	41.0
31	37.1874805716249	39.0	36.0	41.0	32.0	41.0
32	37.09257771109441	39.0	35.0	41.0	31.0	41.0
33	37.02781517756693	39.0	35.0	41.0	31.0	41.0
34	36.88959870961906	39.0	35.0	41.0	31.0	41.0
35	36.82953940675968	39.0	35.0	41.0	31.0	41.0
36	36.78755968059992	39.0	35.0	41.0	31.0	41.0
37	36.71001363715543	39.0	35.0	40.0	31.0	41.0
38	36.646388656042454	39.0	35.0	40.0	31.0	41.0
39	36.528363262253954	39.0	35.0	40.0	30.0	41.0
40	36.443608870599085	39.0	35.0	40.0	30.0	41.0
41	36.39545922182561	38.0	35.0	40.0	30.0	41.0
42	36.29899342972053	38.0	35.0	40.0	30.0	41.0
43	36.248138484976515	38.0	35.0	40.0	30.0	41.0
44	36.213348821497625	38.0	35.0	40.0	30.0	41.0
45	36.059068997077205	38.0	35.0	40.0	30.0	41.0
46	35.836162618025874	38.0	35.0	40.0	29.0	41.0
47	35.756227906990176	38.0	34.0	40.0	29.0	41.0
48	35.716404910915784	38.0	34.0	40.0	29.0	41.0
49	35.54714731303768	38.0	34.0	40.0	28.0	41.0
50	35.3798347180612	37.0	34.0	40.0	28.0	41.0
51	34.10761515312206	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	8.0
10	11.0
11	5.0
12	15.0
13	17.0
14	28.0
15	46.0
16	95.0
17	164.0
18	305.0
19	615.0
20	1125.0
21	1742.0
22	2521.0
23	3357.0
24	4238.0
25	5213.0
26	6544.0
27	8560.0
28	10935.0
29	14037.0
30	18698.0
31	24354.0
32	32525.0
33	44531.0
34	68740.0
35	82694.0
36	96315.0
37	137697.0
38	208873.0
39	264992.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.478926889641055	34.54896816681792	20.42830484479916	24.54380009874186
2	22.60206934450226	32.57624825204774	19.832292822544712	24.98938958090529
3	20.59364452738162	29.532381266763743	23.458024604623546	26.415949601231098
4	23.10251541518257	29.249051799055504	19.424333035311285	28.224099750450645
5	18.928025268676983	34.454268371904575	17.832529572031994	28.78517678738645
6	21.470483786990904	34.72489420858785	21.135184919635098	22.66943708478615
7	82.24080502524846	6.915394779770045	5.898623099628226	4.945177095353262
8	83.47045876468738	4.349165073098811	4.887337078338096	7.293039083875724
9	78.11924667468023	7.441729310645162	8.431265175786494	6.007758838888124
10	39.96802919525385	29.30304223091159	14.442969839462675	16.285958734371885
11	28.93386701415589	26.885695230267746	23.53184040004889	20.648597355527475
12	29.32354127188369	21.95543527740592	28.599819261976783	20.121204188733614
13	27.095208902550638	23.422127223015128	25.79106569028355	23.69159818415068
14	21.66710134899088	24.294924418207383	30.037061881119037	24.0009123516827
15	21.006031337547988	27.775911798304836	27.51240769416586	23.705649169981317
16	23.04323180373275	25.434497864923834	28.59529599941486	22.926974331928555
17	22.39457670442789	25.919160636451917	27.76397808431169	23.922284574808508
18	23.28383087617521	26.140992981243862	26.15870107297562	24.416475069605312
19	24.23949039191664	28.004962115270054	26.415275923828258	21.34027156898505
20	26.17255957954831	27.166137509106676	25.373481940152427	21.28782097119259
21	25.445276703369252	26.04889165631289	25.828021707810713	22.67780993250715
22	24.232657378259276	25.72158067816217	26.06688846693158	23.97887347664697
23	25.720810761130352	25.600318745651172	24.079636368185874	24.599234125032602
24	23.167477164742035	27.758107466944093	25.042995054245466	24.031420314068406
25	23.49488438252173	25.925416212335417	24.55977587715204	26.019923527990812
26	25.831486334453885	27.50278373126816	23.56850769868912	23.097222235588838
27	22.35203878842006	26.299307170910996	25.396675690735876	25.951978349933064
28	24.267688603206896	28.419369957644943	24.54245274393618	22.77048869521198
29	23.679279511641628	28.618393510369337	24.73839662853332	22.963930349455715
30	24.555541333477052	25.350480668826926	24.391452766071296	25.70252523162473
31	26.600248490722016	27.73241148600724	22.029828510605125	23.637511512665615
32	24.977648346170096	25.878547513023626	24.86716525210452	24.276638888701758
33	25.744966908003576	26.602654481446443	24.51155982303457	23.14081878751541
34	24.93385931498557	27.06508589868084	25.03500716504038	22.96604762129321
35	25.026826796577335	26.794845020513474	24.935302909420223	23.243025273488964
36	24.864085583977257	28.612330413743788	24.50126218273403	22.02232181954492
37	25.578761068760326	26.69956778782626	23.80602710300431	23.915644040409095
38	26.349640496865955	25.530641254271835	26.302194359780305	21.8175238890819
39	24.994875239756976	24.174528642357178	27.061525032408696	23.76907108547715
40	25.859780785373115	25.32391853122928	26.233575504319717	22.58272517907789
41	24.940018651240095	24.190504420767354	28.155384655361075	22.71409227263147
42	25.865170204595827	25.421216796125005	24.30839796626416	24.405215033015004
43	23.74520365749086	24.409353337061017	26.709672948868846	25.135770056579275
44	24.1706790571981	23.14206990269211	25.613888533336926	27.073362506772863
45	26.369947058580102	24.201379498841757	25.869308508641836	23.559364933936305
46	21.528901241779934	25.03539212355628	28.9308835856576	24.50482304900618
47	23.907367432317077	26.01309051433345	25.704834982720175	24.374707070629302
48	23.902747930126182	23.500754999889324	29.05358911260325	23.542907957381242
49	24.241896382641066	24.767268517226412	26.070738052090665	24.92009704804186
50	22.362913866494463	23.949327910551037	26.5033351843422	27.184423038612305
51	22.567038119554642	23.478523645595644	28.90085682141678	25.053581413432934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	947.0
1	798.5
2	650.0
3	517.0
4	384.0
5	405.5
6	427.0
7	445.0
8	463.0
9	491.5
10	520.0
11	604.0
12	688.0
13	843.0
14	998.0
15	1009.5
16	1021.0
17	1186.0
18	1351.0
19	1738.5
20	2126.0
21	2251.0
22	2376.0
23	3189.0
24	4002.0
25	4686.5
26	6797.5
27	8224.0
28	10544.5
29	12865.0
30	14366.5
31	15868.0
32	18751.5
33	21635.0
34	22578.5
35	23522.0
36	27727.5
37	31933.0
38	32521.5
39	33110.0
40	35914.0
41	38718.0
42	43891.5
43	49065.0
44	62991.0
45	76917.0
46	82286.5
47	87656.0
48	87147.0
49	86638.0
50	81836.5
51	77035.0
52	71208.5
53	65382.0
54	62836.0
55	60290.0
56	57256.5
57	54223.0
58	53875.5
59	53528.0
60	52973.0
61	52418.0
62	48069.0
63	43720.0
64	38907.5
65	34095.0
66	29539.0
67	24983.0
68	22288.5
69	19594.0
70	17662.5
71	15731.0
72	13468.5
73	11206.0
74	9423.5
75	6364.0
76	5087.0
77	3826.0
78	2565.0
79	2242.0
80	1919.0
81	1459.0
82	999.0
83	722.5
84	446.0
85	420.0
86	394.0
87	300.0
88	206.0
89	155.5
90	105.0
91	62.0
92	19.0
93	12.5
94	6.0
95	4.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1039073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.889437040494773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80724676562824	18.6191025854586
2	9.2842043716313	4.621572403576072
3	3.241006211497944	2.420004601467917
4	1.5568311375435706	1.5499460232229023
5	0.9539953993581229	1.1872204214622835
6	0.7330368968885297	1.0946925416080031
7	0.5621080351773157	0.9793386786051415
8	0.4504428938434952	0.8969016037324756
9	0.4146291403740253	0.9287897296044395
>10	6.715280860890966	41.47819994647787
>50	1.2065388978588163	19.385467900357053
>100	0.06884506201801098	2.8352377977886154
>500	0.0023337309158647773	0.4275074304310622
>1k	0.0023337309158647773	1.1600592252842885
>5k	0.0011668654579323886	2.415959110923367
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	9357	0.9005142083376241	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC	8255	0.7944581372049895	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGT	7344	0.7067838352069585	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	4033	0.3881344236641699	Illumina Single End Adapter 2 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	2009	0.19334541461475757	No Hit
AATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	1948	0.18747479724716165	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	1714	0.16495472406654776	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTAT	1175	0.11308156404795427	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGTC	1104	0.10624855039058853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.623962897698237E-5	0.0	0.0	0.42932498486631837	0.0
2	9.623962897698237E-5	0.0	0.0	0.5759941794272395	0.0
3	9.623962897698237E-5	0.0	0.0	0.9205320511648364	0.0
4	9.623962897698237E-5	0.0	0.0	2.477208049867526	0.0
5	9.623962897698237E-5	0.0	0.0	2.7145349749247645	0.0
6	9.623962897698237E-5	0.0	0.0	3.6808770894826446	0.0
7	9.623962897698237E-5	0.0	0.0	4.425194379990626	0.0
8	9.623962897698237E-5	0.0	0.0	4.952683786413466	0.0
9	9.623962897698237E-5	0.0	0.0	6.389348967781859	0.0
10	9.623962897698237E-5	0.0	0.0	7.442402988048	0.0
11	9.623962897698237E-5	0.0	0.0	9.234962317373274	0.0
12	9.623962897698237E-5	0.0	0.0	9.72020252667522	0.0
13	9.623962897698237E-5	0.0	0.0	9.990828363358494	0.0
14	9.623962897698237E-5	0.0	0.0	10.365489238965885	0.0
15	9.623962897698237E-5	0.0	0.0	10.610130375825376	0.0
16	9.623962897698237E-5	0.0	0.0	11.000574550584993	0.0
17	9.623962897698237E-5	0.0	0.0	11.448954981988754	0.0
18	9.623962897698237E-5	0.0	0.0	12.083559095462975	0.0
19	9.623962897698237E-5	0.0	0.0	12.381805705662643	0.0
20	9.623962897698237E-5	0.0	0.0	12.688136444696378	0.0
21	9.623962897698237E-5	0.0	0.0	13.059428933289576	0.0
22	9.623962897698237E-5	0.0	0.0	13.455070048013951	0.0
23	9.623962897698237E-5	0.0	0.0	13.841087199840628	0.0
24	9.623962897698237E-5	0.0	0.0	14.153192316612982	0.0
25	9.623962897698237E-5	0.0	0.0	14.433730835080885	0.0
26	9.623962897698237E-5	0.0	0.0	14.69377031257669	0.0
27	9.623962897698237E-5	0.0	0.0	14.964492388888942	0.0
28	9.623962897698237E-5	0.0	0.0	15.219431166048969	0.0
29	9.623962897698237E-5	0.0	0.0	15.49939224674301	0.0
30	9.623962897698237E-5	0.0	0.0	15.867605067208945	0.0
31	9.623962897698237E-5	0.0	0.0	16.14900974233764	0.0
32	9.623962897698237E-5	0.0	0.0	16.453511928420813	0.0
33	9.623962897698237E-5	0.0	0.0	16.75464572748979	0.0
34	9.623962897698237E-5	0.0	0.0	17.019497186434446	0.0
35	9.623962897698237E-5	0.0	0.0	17.32303697622785	0.0
36	9.623962897698237E-5	0.0	0.0	17.620417429766725	0.0
37	9.623962897698237E-5	0.0	0.0	17.925304574365803	0.0
38	9.623962897698237E-5	0.0	0.0	18.236543534477367	0.0
39	9.623962897698237E-5	0.0	0.0	18.550669683458235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	45	3.8562575E-10	45.000004	1
ATTCGCT	20	7.032972E-4	45.000004	25
TAAATCG	20	7.032972E-4	45.000004	44
GCTCGAA	20	7.032972E-4	45.000004	21
TTCGATA	20	7.032972E-4	45.000004	43
TATCGAG	20	7.032972E-4	45.000004	1
TAATGCG	20	7.032972E-4	45.000004	1
ACGGTAC	20	7.032972E-4	45.000004	13
TAATCCG	60	0.0	45.000004	1
AGCGTAA	25	3.890546E-5	45.0	11
CGGTTTA	25	3.890546E-5	45.0	43
ATACGGT	50	2.1827873E-11	45.0	27
TAATCGC	25	3.890546E-5	45.0	30
TCAACCG	80	0.0	42.187504	1
CATACCG	70	0.0	41.785713	1
TCGTTGA	200	0.0	41.625	24
TCACGCG	65	0.0	41.53846	1
ATATACG	60	3.6379788E-12	41.250004	1
CATAACG	60	3.6379788E-12	41.250004	1
TATCGCG	50	1.0804797E-9	40.5	1
>>END_MODULE