FastQCFastQC Report
Sat 18 Jun 2016
SRR3554065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145247
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC18820.1643313625794261Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC14570.12722146401605944Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT12690.11080579123979369Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.10058965445881979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA502.1827873E-1145.00000426
TAATGCG351.2119199E-745.0000041
CCCATCG253.890796E-545.00000411
AACGAAG406.8157533E-945.01
CATCGAA207.0332765E-445.014
CAATCGA207.0332765E-445.043
GTTTACG556.184564E-1140.9090921
ACGGGTA1550.040.6451645
TCGACGT501.0822987E-940.50000426
CTAGACG451.9283107E-840.01
CGTAAGG1650.039.5454522
CGTTATG356.2482686E-638.571432
CAAATCG356.2482686E-638.5714337
CGTTTAT356.2482686E-638.5714339
ATTGATC659.094947E-1238.0769212
ACACGAC7050.037.9787226
CGTGATT301.1399194E-437.49999615
TACGCAT601.5643309E-1037.49999616
GTATACG301.1399194E-437.4999961
TACGTAG301.1399194E-437.4999961