##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1145247
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11574097116168	33.0	31.0	34.0	30.0	34.0
2	32.234206245464954	34.0	31.0	34.0	30.0	34.0
3	32.24200630955593	34.0	31.0	34.0	30.0	34.0
4	35.806942083236194	37.0	35.0	37.0	35.0	37.0
5	35.6858834819039	37.0	35.0	37.0	33.0	37.0
6	35.67401791927855	37.0	35.0	37.0	33.0	37.0
7	35.898655923132736	37.0	35.0	37.0	35.0	37.0
8	35.76686950500634	37.0	35.0	37.0	35.0	37.0
9	37.45025832855271	39.0	37.0	39.0	35.0	39.0
10	37.02283175594435	39.0	37.0	39.0	33.0	39.0
11	36.9848399515563	39.0	37.0	39.0	33.0	39.0
12	36.80585760102406	39.0	35.0	39.0	33.0	39.0
13	36.7334391620323	39.0	35.0	39.0	33.0	39.0
14	37.7559329996062	40.0	36.0	41.0	33.0	41.0
15	37.86946833303209	40.0	37.0	41.0	33.0	41.0
16	37.97408157366926	40.0	37.0	41.0	33.0	41.0
17	37.92808887515095	40.0	36.0	41.0	33.0	41.0
18	37.89731909361037	40.0	36.0	41.0	33.0	41.0
19	37.873229966985285	40.0	36.0	41.0	33.0	41.0
20	37.82779959257697	40.0	36.0	41.0	33.0	41.0
21	37.696468971322346	40.0	36.0	41.0	33.0	41.0
22	37.71238082265223	40.0	36.0	41.0	33.0	41.0
23	37.66533944205922	40.0	36.0	41.0	33.0	41.0
24	37.62790908860708	40.0	35.0	41.0	33.0	41.0
25	37.53828737381543	39.0	35.0	41.0	33.0	41.0
26	37.492632593667565	39.0	35.0	41.0	33.0	41.0
27	37.45348994583701	39.0	35.0	41.0	33.0	41.0
28	37.33925258044771	39.0	35.0	41.0	32.0	41.0
29	37.30034918231613	39.0	35.0	41.0	32.0	41.0
30	37.15213574015038	39.0	35.0	41.0	32.0	41.0
31	37.05705930685695	39.0	35.0	41.0	31.0	41.0
32	36.94633559398104	39.0	35.0	41.0	31.0	41.0
33	36.858468085923825	39.0	35.0	41.0	31.0	41.0
34	36.68540148980962	39.0	35.0	41.0	31.0	41.0
35	36.60940914929269	39.0	35.0	41.0	30.0	41.0
36	36.5177677828451	39.0	35.0	41.0	30.0	41.0
37	36.36880341096724	39.0	35.0	41.0	30.0	41.0
38	36.24197050941849	38.0	35.0	41.0	30.0	41.0
39	36.16534773721302	38.0	35.0	40.0	30.0	41.0
40	36.01803366435363	38.0	35.0	40.0	29.0	41.0
41	35.91853547749962	38.0	35.0	40.0	29.0	41.0
42	35.8200964508093	38.0	35.0	40.0	29.0	41.0
43	35.72515710584704	38.0	35.0	40.0	29.0	41.0
44	35.5914112850765	38.0	34.0	40.0	28.0	41.0
45	35.53867157041232	38.0	34.0	40.0	28.0	41.0
46	35.4418758573478	38.0	34.0	40.0	28.0	41.0
47	35.316548307919604	37.0	34.0	40.0	28.0	41.0
48	35.20912170038429	37.0	34.0	40.0	27.0	41.0
49	35.12537295448056	37.0	34.0	40.0	27.0	41.0
50	34.997270457813904	37.0	34.0	40.0	27.0	41.0
51	33.477322359281445	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	9.0
11	14.0
12	12.0
13	30.0
14	40.0
15	69.0
16	154.0
17	267.0
18	426.0
19	816.0
20	1274.0
21	2043.0
22	3058.0
23	4019.0
24	5812.0
25	7705.0
26	9690.0
27	12705.0
28	15067.0
29	18146.0
30	22064.0
31	27435.0
32	35546.0
33	49367.0
34	84608.0
35	97343.0
36	95965.0
37	137949.0
38	218553.0
39	294948.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35510680228806	19.366215322982725	24.061883593670185	11.21679428105902
2	32.19584945430986	24.321914835838907	24.963261200422266	18.518974509428972
3	33.23641101002666	24.704015814929008	25.031892683412398	17.027680491631937
4	28.047050112333842	26.601772368755388	25.281882423616914	20.069295095293853
5	25.17666494651372	30.41405041881795	24.313095777592082	20.096188857076246
6	25.686511294070186	35.29081499449463	23.965485174813818	15.057188536621357
7	83.61864296522934	5.387571414725382	6.792159245996715	4.201626374048568
8	84.9105913396848	4.543386710465079	6.347451685095005	4.198570264755114
9	79.1885942508472	6.75784350450165	8.560074813555504	5.49348743109565
10	41.848701633796026	24.642195089792857	16.712290012547513	16.7968132638636
11	33.91381946427277	26.32550008862717	20.763468491949773	18.997211955150288
12	28.740699604539454	24.018312206886375	27.52829738912217	19.712690799451995
13	26.382081769260257	25.328247967469025	28.207539508944357	20.08213075432636
14	22.948499319360803	27.055560940129077	29.104114658235297	20.891825082274828
15	21.3238716189608	26.192079088615817	31.091371555655677	21.392677736767702
16	25.58950165335513	24.3722969804767	29.277963618328624	20.76023774783955
17	25.634819388306628	24.259832158477604	26.669268725436524	23.436079727779248
18	27.25246169603588	23.14574934490114	27.39767054617912	22.204118412883858
19	26.595922102393633	26.666125298734684	24.671533739010012	22.066418859861674
20	28.05106671311953	26.211201601051997	24.6839328110006	21.053798874827876
21	26.032200913863996	27.113539699296307	25.977889485848905	20.87636990099079
22	24.83656364085651	24.434554292654774	26.556891220845806	24.171990845642906
23	23.366837022930426	27.43967021961201	26.422422411933844	22.771070345523718
24	23.836604680038455	25.570641093144097	28.136812408153	22.455941818664446
25	24.655118066233747	26.237134871342167	25.866821742383955	23.24092532004013
26	23.32099538352862	26.161168726047745	26.090703577481538	24.4271323129421
27	23.626955582507527	25.466471424941517	26.966584500985373	23.939988491565575
28	21.60852637029392	26.750473915234007	27.761784139142037	23.879215575330036
29	23.74107943526593	26.783479895603307	26.66071161941485	22.81472904971591
30	24.626041369241744	24.84285049426019	28.096908352521332	22.434199783976734
31	26.64621692962304	26.42250972934223	24.885723341776927	22.0455499992578
32	25.97238848912069	26.33231084648115	26.100483127220592	21.59481753717757
33	26.205176699873476	25.748943241065025	24.457256818834715	23.58862324022678
34	23.7714658933837	26.82416980791043	27.576321963733584	21.82804233497228
35	23.86576869443884	27.316727308606794	27.023166181618464	21.794337815335908
36	26.16413751793281	28.016838289032847	25.152739976616402	20.666284216417942
37	24.881444788766093	28.737032273387314	24.57085676714281	21.810666170703787
38	25.20879775279918	28.89219530808638	24.2430672160678	21.655939723046643
39	25.911964842518685	26.205525969507015	23.793120610663028	24.08938857731127
40	26.49402268680905	24.713708047259676	26.816485875972607	21.97578338995867
41	23.360986756568668	25.596574363434264	26.1312188549719	24.91122002502517
42	25.198756250834975	25.316896704379054	26.575751781056837	22.908595263729133
43	24.563172835204984	25.046212738387442	26.361212908656384	24.029401517751193
44	24.266031694472893	24.967015848982797	26.883807597836974	23.883144858707336
45	24.562037708895986	25.02045410291404	25.968721157968545	24.44878703022143
46	24.74304669647683	25.04210882019337	26.226394830110884	23.988449653218915
47	23.256467818732553	26.577323494407757	27.231592835432007	22.934615851427683
48	23.71165346864039	26.407491135100113	26.892539338675412	22.988316057584086
49	25.393823341165707	25.040711741659223	27.302319936223363	22.263144980951708
50	22.581678886737972	25.774789193946805	27.33628640808489	24.30724551123033
51	22.822238346836972	25.524624818925524	25.901137483879022	25.751999350358485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1768.0
1	1773.5
2	1779.0
3	2301.5
4	2824.0
5	2366.5
6	1909.0
7	2314.5
8	2720.0
9	3042.0
10	3364.0
11	3408.0
12	3452.0
13	3250.5
14	3049.0
15	2758.0
16	2467.0
17	2388.5
18	2310.0
19	2576.5
20	2843.0
21	3216.5
22	3590.0
23	3846.5
24	4103.0
25	4791.5
26	7025.0
27	8570.0
28	9904.5
29	11239.0
30	12793.5
31	14348.0
32	17417.5
33	20487.0
34	23044.5
35	25602.0
36	28273.5
37	30945.0
38	32943.0
39	34941.0
40	39521.0
41	44101.0
42	49511.0
43	54921.0
44	59842.0
45	64763.0
46	72269.0
47	79775.0
48	87584.0
49	95393.0
50	96115.5
51	96838.0
52	92255.0
53	87672.0
54	82393.5
55	77115.0
56	72811.0
57	68507.0
58	63766.0
59	59025.0
60	55943.0
61	52861.0
62	50750.0
63	48639.0
64	41393.5
65	34148.0
66	30706.0
67	27264.0
68	23653.0
69	20042.0
70	17267.5
71	14493.0
72	13178.5
73	11864.0
74	9781.5
75	6840.0
76	5981.0
77	4422.5
78	2864.0
79	2252.5
80	1641.0
81	1297.0
82	953.0
83	754.0
84	555.0
85	366.0
86	177.0
87	126.0
88	75.0
89	59.5
90	44.0
91	30.5
92	17.0
93	15.0
94	13.0
95	12.5
96	12.0
97	8.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1145247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.48866752816213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95703310556176	19.855119288306675
2	9.401895601863556	4.980873734645069
3	3.3094718306770297	2.6299049704986572
4	1.6410770888358672	1.7387978157702988
5	0.9940369091724688	1.316535659889571
6	0.727897178488544	1.1568615813402194
7	0.5806564268662667	1.076657052754579
8	0.46479067141123087	0.9849348452162676
9	0.4116537303469272	0.9813742919898715
>10	6.280427519932406	41.854290529675005
>50	1.1638631094374865	19.138730424234886
>100	0.06024543146786978	2.7949668520018207
>500	0.005627320521718857	0.9859018472798057
>1k	0.0013240754168750252	0.5050511063973391
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	1882	0.1643313625794261	Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	1457	0.12722146401605944	Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	1269	0.11080579123979369	Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.10058965445881979	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6195222515317657E-4	0.0	0.0	0.11639410537639479	0.0
2	2.6195222515317657E-4	0.0	0.0	0.5360415700717837	0.0
3	3.4926963353756874E-4	0.0	0.0	0.7401896708744926	0.0
4	3.4926963353756874E-4	0.0	0.0	1.0076428927558858	0.0
5	3.4926963353756874E-4	0.0	0.0	1.8227509000241868	0.0
6	3.4926963353756874E-4	0.0	0.0	2.4242805263842646	0.0
7	3.4926963353756874E-4	0.0	0.0	2.9532493863769127	0.0
8	3.4926963353756874E-4	0.0	0.0	3.7777003563423435	0.0
9	3.4926963353756874E-4	0.0	0.0	4.1153567745647885	0.0
10	3.4926963353756874E-4	0.0	0.0	4.837733694128865	0.0
11	3.4926963353756874E-4	0.0	0.0	5.785214892507905	0.0
12	3.4926963353756874E-4	0.0	0.0	6.489604425944796	0.0
13	3.4926963353756874E-4	0.0	0.0	6.780109443639669	0.0
14	3.4926963353756874E-4	0.0	0.0	6.904449433179043	0.0
15	3.4926963353756874E-4	0.0	0.0	7.144223036602584	0.0
16	3.4926963353756874E-4	0.0	0.0	7.638701520283397	0.0
17	3.4926963353756874E-4	0.0	0.0	8.229403788003811	0.0
18	3.4926963353756874E-4	0.0	0.0	8.849881291983301	0.0
19	3.4926963353756874E-4	0.0	0.0	9.215391963480368	0.0
20	3.4926963353756874E-4	0.0	0.0	9.575401638249216	0.0
21	3.4926963353756874E-4	0.0	0.0	10.012687219438252	0.0
22	5.239044503063531E-4	0.0	0.0	10.451631831386592	0.0
23	5.239044503063531E-4	0.0	0.0	10.87206515275744	0.0
24	6.112218586907454E-4	0.0	0.0	11.226573830798072	0.0
25	6.112218586907454E-4	0.0	0.0	11.544671149542413	0.0
26	6.112218586907454E-4	0.0	0.0	11.83744641985528	0.0
27	6.112218586907454E-4	0.0	0.0	12.18654141857608	0.0
28	6.985392670751375E-4	0.0	0.0	12.515728048185238	0.0
29	6.985392670751375E-4	0.0	0.0	12.856615210517905	0.0
30	6.985392670751375E-4	0.0	0.0	13.270063139218003	0.0
31	6.985392670751375E-4	0.0	0.0	13.588771679821035	0.0
32	6.985392670751375E-4	0.0	0.0	13.919180753147574	0.0
33	6.985392670751375E-4	0.0	0.0	14.251860079092108	0.0
34	6.985392670751375E-4	0.0	0.0	14.593358463283467	0.0
35	6.985392670751375E-4	0.0	0.0	14.971879428629807	0.0
36	6.985392670751375E-4	0.0	0.0	15.296350918186208	0.0
37	8.731740838439219E-4	0.0	0.0	15.659242067431741	0.0
38	8.731740838439219E-4	0.0	0.0	16.03156349678279	0.0
39	8.731740838439219E-4	0.0	0.0	16.567867019079728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTA	50	2.1827873E-11	45.000004	26
TAATGCG	35	1.2119199E-7	45.000004	1
CCCATCG	25	3.890796E-5	45.000004	11
AACGAAG	40	6.8157533E-9	45.0	1
CATCGAA	20	7.0332765E-4	45.0	14
CAATCGA	20	7.0332765E-4	45.0	43
GTTTACG	55	6.184564E-11	40.909092	1
ACGGGTA	155	0.0	40.645164	5
TCGACGT	50	1.0822987E-9	40.500004	26
CTAGACG	45	1.9283107E-8	40.0	1
CGTAAGG	165	0.0	39.545452	2
CGTTATG	35	6.2482686E-6	38.57143	2
CAAATCG	35	6.2482686E-6	38.57143	37
CGTTTAT	35	6.2482686E-6	38.57143	39
ATTGATC	65	9.094947E-12	38.07692	12
ACACGAC	705	0.0	37.97872	26
CGTGATT	30	1.1399194E-4	37.499996	15
TACGCAT	60	1.5643309E-10	37.499996	16
GTATACG	30	1.1399194E-4	37.499996	1
TACGTAG	30	1.1399194E-4	37.499996	1
>>END_MODULE