FastQCFastQC Report
Sat 18 Jun 2016
SRR3554064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636883
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC17510.2749327584501392No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT13900.2182504478844623Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC13700.21511015367029737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11100.17428632888615336No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT11050.17350125533261213No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC10760.16894782872207298No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCG10580.16612156392932453No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGC9640.1513621811227494No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC9490.1490069604621257No Hit
ACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC7510.11791804774189295No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7230.11352163584206205No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCC7010.11006731220648064No Hit
TCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC6970.10943925336364764No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6900.10834015038868991No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.10268762080319306No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG253.888839E-545.0000041
GACGTGA253.888839E-545.00000423
GGATCGT351.2107921E-745.0000048
GTTAGCG453.8380676E-1045.0000041
CTACGTG253.888839E-545.00000430
CACTAGC302.1639717E-645.00000438
ACGGACT351.2107921E-745.0000045
TCGTAAG351.2107921E-745.0000041
ATGCGTC253.888839E-545.0000041
CGTAAGG1450.045.0000042
CTCGAAT253.888839E-545.00000415
CGCAATA253.888839E-545.00000410
TATACGG302.1639717E-645.0000042
TGAGCGT351.2107921E-745.00000415
TCGACAG253.888839E-545.0000041
GCGTACG302.1639717E-645.0000041
TACGTGA253.888839E-545.00000431
TATCCGA253.888839E-545.00000430
CTTCGTA207.0309156E-445.032
TCGTTTA406.8066583E-945.038