##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	636883
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.007370270520646	33.0	31.0	34.0	30.0	34.0
2	32.1452103447572	33.0	31.0	34.0	30.0	34.0
3	32.14950312694796	34.0	31.0	34.0	30.0	34.0
4	35.72429315902607	37.0	35.0	37.0	33.0	37.0
5	35.61530924832348	37.0	35.0	37.0	33.0	37.0
6	35.60684144497498	37.0	35.0	37.0	33.0	37.0
7	35.813073986901834	37.0	35.0	37.0	35.0	37.0
8	35.641331296329156	37.0	35.0	37.0	35.0	37.0
9	37.373326026915464	39.0	37.0	39.0	35.0	39.0
10	36.92296701277943	39.0	37.0	39.0	33.0	39.0
11	36.92591260875231	39.0	37.0	39.0	33.0	39.0
12	36.75506019158935	39.0	35.0	39.0	33.0	39.0
13	36.609939345217256	39.0	35.0	39.0	33.0	39.0
14	37.70025263666953	40.0	36.0	41.0	33.0	41.0
15	37.86700068929458	40.0	36.0	41.0	33.0	41.0
16	37.960870363944395	40.0	36.0	41.0	33.0	41.0
17	37.93115690008997	40.0	36.0	41.0	33.0	41.0
18	37.89760128626451	40.0	36.0	41.0	33.0	41.0
19	37.84165537469205	40.0	36.0	41.0	33.0	41.0
20	37.76580470824312	40.0	36.0	41.0	33.0	41.0
21	37.63362187403338	39.0	35.0	41.0	33.0	41.0
22	37.62059907392724	39.0	35.0	41.0	33.0	41.0
23	37.583213871307606	39.0	35.0	41.0	33.0	41.0
24	37.57335020718091	39.0	35.0	41.0	33.0	41.0
25	37.49093475567726	39.0	35.0	41.0	33.0	41.0
26	37.4526561393537	39.0	35.0	41.0	33.0	41.0
27	37.3914470946783	39.0	35.0	41.0	33.0	41.0
28	37.288125134443845	39.0	35.0	41.0	32.0	41.0
29	37.26684179040734	39.0	35.0	41.0	32.0	41.0
30	37.16151161202293	39.0	35.0	41.0	32.0	41.0
31	37.07599983042411	39.0	35.0	41.0	32.0	41.0
32	36.95903957241754	39.0	35.0	41.0	31.0	41.0
33	36.87393602906656	39.0	35.0	41.0	31.0	41.0
34	36.76536035661181	39.0	35.0	41.0	31.0	41.0
35	36.718441220757974	39.0	35.0	41.0	31.0	41.0
36	36.587151800252165	39.0	35.0	41.0	31.0	41.0
37	36.49263836528844	39.0	35.0	41.0	30.0	41.0
38	36.37058769036071	39.0	35.0	40.0	30.0	41.0
39	36.3346485932267	39.0	35.0	40.0	30.0	41.0
40	36.20022044865384	38.0	35.0	40.0	30.0	41.0
41	36.102783399776726	38.0	35.0	40.0	30.0	41.0
42	35.981166085450546	38.0	35.0	40.0	30.0	41.0
43	35.88166429312762	38.0	35.0	40.0	30.0	41.0
44	35.77462893498492	38.0	35.0	40.0	29.0	41.0
45	35.75596930676435	38.0	35.0	40.0	29.0	41.0
46	35.667917027146274	38.0	35.0	40.0	29.0	41.0
47	35.54316255890014	38.0	34.0	40.0	29.0	41.0
48	35.467296190980136	37.0	34.0	40.0	28.0	41.0
49	35.38280186470671	37.0	34.0	40.0	28.0	41.0
50	35.25350653102689	37.0	34.0	40.0	28.0	41.0
51	33.75259192033702	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	2.0
12	11.0
13	8.0
14	21.0
15	27.0
16	51.0
17	106.0
18	241.0
19	458.0
20	709.0
21	1047.0
22	1657.0
23	2217.0
24	3187.0
25	4017.0
26	4831.0
27	6077.0
28	7451.0
29	9306.0
30	11864.0
31	15246.0
32	19817.0
33	27496.0
34	47069.0
35	62483.0
36	53139.0
37	74924.0
38	121208.0
39	162151.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39077821201068	23.31480036364607	25.187043774131197	11.10737765021205
2	31.09613539692534	24.65853225788724	26.659056687021003	17.586275658166414
3	31.83159230188276	26.07951538979687	26.240612482983533	15.848279825336837
4	26.963822240505714	27.43141204899487	26.50753749118755	19.09722821931187
5	23.610616078620406	31.431989863130276	24.69527370019297	20.262120358056347
6	24.20680093517962	37.44345507730619	24.088097813884183	14.261646173630007
7	80.68954580354634	7.0293915836974765	7.698588280736023	4.582474332020167
8	81.98852850523566	6.085105113498083	7.025466215929771	4.90090016533649
9	75.86118643455706	8.035698864626626	9.499075968427482	6.604038732388838
10	41.27618416569449	24.570604019890624	17.94819456634892	16.20501724806597
11	31.799090256766156	26.891595473579923	21.23309932907614	20.07621494057778
12	27.556081729297215	22.89101765944451	30.08103529219653	19.47186531906174
13	23.640919917787098	28.745468162912186	28.78864720835695	18.824964710943767
14	19.504681393599764	31.42256898048778	28.801836444056445	20.270913181856006
15	18.50858006886665	27.37049034124007	33.62234507751031	20.498584512382966
16	20.71934719563876	23.94066100052914	33.60727166528232	21.73272013854978
17	20.57850500013346	25.811177249196476	26.665808319581462	26.944509431088598
18	21.486521072159253	25.51002303405806	30.66560106016333	22.33785483361936
19	24.05810800413891	28.286043119379855	26.058318403851256	21.59753047262998
20	24.596668461868195	25.955787797758774	27.22085532193511	22.226688418437924
21	23.275546685969008	28.839205945205006	27.243779469698516	20.64146789912747
22	21.66143545988824	25.306217939558756	27.227763969206276	25.80458263134673
23	20.677581282590367	28.63838413020916	25.955316753626644	24.72871783357383
24	21.461869762578058	26.124892641191554	29.65536841146647	22.75786918476392
25	21.044681676226247	28.180686248494624	27.323385928027598	23.45124614725154
26	19.029397864285905	28.216328587825394	28.160745380234676	24.59352816765403
27	21.29778938988794	28.709511794159997	26.92708079819998	23.065618017752083
28	19.124548778975104	27.327939354638136	31.13303385394178	22.414478012444985
29	20.90839290733149	27.034478860324423	28.68438944044668	23.37273879189741
30	20.951257923354838	27.131199922120704	27.968088330195656	23.9494538243288
31	24.016342091090515	28.43960350645252	24.06423157785653	23.47982282460044
32	23.14820775558462	27.310667736460232	26.83507017772495	22.7060543302302
33	23.3229651286029	27.010926653718187	23.943958309454015	25.7221499082249
34	20.068050175620954	26.34973770692576	29.01600450946249	24.56620760799079
35	22.35214317229381	24.9623557231077	27.649505482168625	25.035995622429862
36	24.8595110875938	26.142007244658753	26.348324574529386	22.650157093218063
37	22.38276104088192	28.793357649678196	27.01485202148589	21.809029287953987
38	21.988497102293515	27.92742152012222	26.417411047241018	23.66667033034325
39	22.845326378628414	25.75732120342355	28.097782481240667	23.29956993670737
40	23.09984722468648	23.49128489848214	29.7644936354087	23.64437424142268
41	22.13734704804493	24.938803516501462	26.8435489721032	26.080300463350408
42	23.50651532542084	25.093620021259795	27.388075988839393	24.011788664479976
43	22.75331575815338	24.778177467446923	27.885498592363117	24.583008182036576
44	21.442242923739524	25.147633081743432	28.24160795624942	25.168516038267626
45	21.241264094032967	25.585233080487313	27.511646566166785	25.661856259312938
46	23.09011231262257	25.37954380945951	28.05177717100315	23.478566706914773
47	21.1751609008248	25.387237530284214	30.00362703981736	23.433974529073627
48	22.24662928669787	24.285151275823033	28.801522414635027	24.66669702284407
49	22.136090930359266	23.666513315632542	30.113537337313133	24.083858416695062
50	21.154748988432726	23.332543025956102	30.56401254233509	24.94869544327608
51	20.680093517961698	24.850090204951304	27.115812480471295	27.354003796615707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2948.0
1	2393.0
2	1838.0
3	1778.5
4	1719.0
5	1637.5
6	1556.0
7	2074.5
8	2593.0
9	2982.5
10	3372.0
11	3278.0
12	3184.0
13	2865.0
14	2546.0
15	2432.5
16	2319.0
17	2347.5
18	2376.0
19	2272.5
20	2169.0
21	2335.5
22	2502.0
23	2771.5
24	3041.0
25	3457.0
26	4277.0
27	4681.0
28	6306.5
29	7932.0
30	8954.0
31	9976.0
32	10298.5
33	10621.0
34	12137.5
35	13654.0
36	15436.5
37	17219.0
38	18179.0
39	19139.0
40	22767.5
41	26396.0
42	30727.5
43	35059.0
44	40461.5
45	45864.0
46	51209.0
47	56554.0
48	58678.0
49	60802.0
50	59100.0
51	57398.0
52	50923.5
53	44449.0
54	40280.5
55	36112.0
56	32873.0
57	29634.0
58	27949.5
59	26265.0
60	24835.5
61	23406.0
62	21838.0
63	20270.0
64	17836.5
65	15403.0
66	13734.0
67	12065.0
68	10428.5
69	8792.0
70	7572.5
71	6353.0
72	5539.5
73	4726.0
74	4258.0
75	2868.0
76	1946.0
77	1533.5
78	1121.0
79	911.5
80	702.0
81	470.0
82	238.0
83	185.5
84	133.0
85	109.5
86	86.0
87	59.5
88	33.0
89	24.5
90	16.0
91	13.0
92	10.0
93	5.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	636883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.53507660603934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.25525207701006	20.72159130947273
2	10.248791320600544	5.64402508264094
3	3.754009564388806	3.1010082280575033
4	1.790231971341207	1.971766978938438
5	1.1170451754927333	1.537896223979953
6	0.6812218706241312	1.1254497836006923
7	0.501036451366746	0.9657253949561018
8	0.40237886717106286	0.8863626345765233
9	0.31979674570371097	0.7925065102182386
>10	4.425684979922316	30.755603509771085
>50	1.4032170635860686	25.787623055102415
>100	0.08988375315504393	3.892774788169446
>500	0.007442603764430389	1.4209715696347043
>1k	0.004007555873154825	1.396694930881238
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	1751	0.2749327584501392	No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT	1390	0.2182504478844623	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	1370	0.21511015367029737	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1110	0.17428632888615336	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	1105	0.17350125533261213	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1076	0.16894782872207298	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCG	1058	0.16612156392932453	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGC	964	0.1513621811227494	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC	949	0.1490069604621257	No Hit
ACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	751	0.11791804774189295	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	723	0.11352163584206205	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCC	701	0.11006731220648064	No Hit
TCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	697	0.10943925336364764	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	690	0.10834015038868991	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	654	0.10268762080319306	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5701471070824627E-4	0.0	0.0	0.22311790391641792	0.0
2	1.5701471070824627E-4	0.0	0.0	0.9664255444092557	0.0
3	1.5701471070824627E-4	0.0	0.0	1.309031643174649	0.0
4	1.5701471070824627E-4	0.0	0.0	1.714443626223341	0.0
5	1.5701471070824627E-4	0.0	0.0	3.072620873849671	0.0
6	1.5701471070824627E-4	0.0	0.0	3.9076251053961246	0.0
7	1.5701471070824627E-4	0.0	0.0	4.69144254125169	0.0
8	1.5701471070824627E-4	0.0	0.0	5.9397094913822475	0.0
9	1.5701471070824627E-4	0.0	0.0	6.407299299871405	0.0
10	1.5701471070824627E-4	0.0	0.0	7.532466716806698	0.0
11	1.5701471070824627E-4	0.0	0.0	8.854216551548715	0.0
12	1.5701471070824627E-4	0.0	0.0	10.019736749136026	0.0
13	1.5701471070824627E-4	0.0	0.0	10.410389349378143	0.0
14	1.5701471070824627E-4	0.0	0.0	10.578709119257383	0.0
15	1.5701471070824627E-4	0.0	0.0	10.932934306615186	0.0
16	1.5701471070824627E-4	0.0	0.0	11.719420992552791	0.0
17	1.5701471070824627E-4	0.0	0.0	12.679094904401593	0.0
18	1.5701471070824627E-4	0.0	0.0	13.774743555723736	0.0
19	1.5701471070824627E-4	0.0	0.0	14.308279542710357	0.0
20	1.5701471070824627E-4	0.0	0.0	14.81025557284462	0.0
21	1.5701471070824627E-4	0.0	0.0	15.354939604291527	0.0
22	1.5701471070824627E-4	0.0	0.0	15.962900564153855	0.0
23	1.5701471070824627E-4	0.0	0.0	16.5292526256785	0.0
24	1.5701471070824627E-4	0.0	0.0	17.005007199124485	0.0
25	1.5701471070824627E-4	0.0	0.0	17.42737677092967	0.0
26	1.5701471070824627E-4	0.0	0.0	17.809393562082832	0.0
27	1.5701471070824627E-4	0.0	0.0	18.222970310088353	0.0
28	1.5701471070824627E-4	0.0	0.0	18.61346589561976	0.0
29	1.5701471070824627E-4	0.0	0.0	19.014481466768622	0.0
30	1.5701471070824627E-4	0.0	0.0	19.449412215430463	0.0
31	1.5701471070824627E-4	0.0	0.0	19.845560330547368	0.0
32	1.5701471070824627E-4	0.0	0.0	20.262120358056347	0.0
33	1.5701471070824627E-4	0.0	0.0	20.667846370526455	0.0
34	1.5701471070824627E-4	0.0	0.0	21.04593779391191	0.0
35	1.5701471070824627E-4	0.0	0.0	21.463596924395848	0.0
36	1.5701471070824627E-4	0.0	0.0	21.84655580381326	0.0
37	1.5701471070824627E-4	0.0	0.0	22.227787521412882	0.0
38	1.5701471070824627E-4	0.0	0.0	22.61749803339075	0.0
39	1.5701471070824627E-4	0.0	0.0	23.005481383550826	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	25	3.888839E-5	45.000004	1
GACGTGA	25	3.888839E-5	45.000004	23
GGATCGT	35	1.2107921E-7	45.000004	8
GTTAGCG	45	3.8380676E-10	45.000004	1
CTACGTG	25	3.888839E-5	45.000004	30
CACTAGC	30	2.1639717E-6	45.000004	38
ACGGACT	35	1.2107921E-7	45.000004	5
TCGTAAG	35	1.2107921E-7	45.000004	1
ATGCGTC	25	3.888839E-5	45.000004	1
CGTAAGG	145	0.0	45.000004	2
CTCGAAT	25	3.888839E-5	45.000004	15
CGCAATA	25	3.888839E-5	45.000004	10
TATACGG	30	2.1639717E-6	45.000004	2
TGAGCGT	35	1.2107921E-7	45.000004	15
TCGACAG	25	3.888839E-5	45.000004	1
GCGTACG	30	2.1639717E-6	45.000004	1
TACGTGA	25	3.888839E-5	45.000004	31
TATCCGA	25	3.888839E-5	45.000004	30
CTTCGTA	20	7.0309156E-4	45.0	32
TCGTTTA	40	6.8066583E-9	45.0	38
>>END_MODULE