##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	687232
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.999445602067425	33.0	31.0	34.0	30.0	34.0
2	32.11548356304712	33.0	31.0	34.0	30.0	34.0
3	32.13154073151425	34.0	31.0	34.0	30.0	34.0
4	35.72290725693798	37.0	35.0	37.0	33.0	37.0
5	35.598385989010985	37.0	35.0	37.0	33.0	37.0
6	35.58948506472341	37.0	35.0	37.0	33.0	37.0
7	35.82581573617061	37.0	35.0	37.0	35.0	37.0
8	35.649930445613705	37.0	35.0	37.0	35.0	37.0
9	37.36419869854721	39.0	37.0	39.0	34.0	39.0
10	36.89613987707208	39.0	37.0	39.0	33.0	39.0
11	36.8677666348482	39.0	37.0	39.0	33.0	39.0
12	36.74776203669212	39.0	35.0	39.0	33.0	39.0
13	36.64824979046377	39.0	35.0	39.0	33.0	39.0
14	37.71781872788229	40.0	36.0	41.0	33.0	41.0
15	37.89828471316819	40.0	36.0	41.0	33.0	41.0
16	37.978979442168004	40.0	36.0	41.0	33.0	41.0
17	37.96537704879866	40.0	36.0	41.0	33.0	41.0
18	37.93645522909294	40.0	36.0	41.0	33.0	41.0
19	37.879359517601046	40.0	36.0	41.0	33.0	41.0
20	37.820102963773515	40.0	36.0	41.0	33.0	41.0
21	37.706470304060346	39.0	36.0	41.0	33.0	41.0
22	37.69701934717825	39.0	36.0	41.0	33.0	41.0
23	37.64952446917489	39.0	36.0	41.0	33.0	41.0
24	37.62788694356491	39.0	35.0	41.0	33.0	41.0
25	37.52699088517415	39.0	35.0	41.0	33.0	41.0
26	37.50173449431924	39.0	35.0	41.0	33.0	41.0
27	37.46282623626374	39.0	35.0	41.0	33.0	41.0
28	37.387869016576644	39.0	35.0	41.0	33.0	41.0
29	37.365463191469544	39.0	35.0	41.0	33.0	41.0
30	37.237793059694546	39.0	35.0	41.0	32.0	41.0
31	37.17239302011548	39.0	35.0	41.0	32.0	41.0
32	37.0884723062954	39.0	35.0	41.0	32.0	41.0
33	36.97510302197802	39.0	35.0	41.0	31.0	41.0
34	36.92712068122555	39.0	35.0	41.0	31.0	41.0
35	36.84404247764947	39.0	35.0	41.0	31.0	41.0
36	36.71569717358912	39.0	35.0	41.0	31.0	41.0
37	36.648306539858446	39.0	35.0	41.0	31.0	41.0
38	36.55214105280313	39.0	35.0	41.0	31.0	41.0
39	36.45788030825107	39.0	35.0	40.0	30.0	41.0
40	36.3170894254051	38.0	35.0	40.0	30.0	41.0
41	36.21117904870553	38.0	35.0	40.0	30.0	41.0
42	36.09485734075247	38.0	35.0	40.0	30.0	41.0
43	36.00800894021233	38.0	35.0	40.0	30.0	41.0
44	35.92161162460421	38.0	35.0	40.0	30.0	41.0
45	35.87503346759173	38.0	35.0	40.0	30.0	41.0
46	35.744445834885454	38.0	35.0	40.0	29.0	41.0
47	35.629611252095366	38.0	34.0	40.0	29.0	41.0
48	35.54017857142857	37.0	34.0	40.0	29.0	41.0
49	35.44808885500093	37.0	34.0	40.0	28.0	41.0
50	35.3659200968523	37.0	34.0	40.0	28.0	41.0
51	33.83945450735705	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	8.0
12	11.0
13	8.0
14	24.0
15	34.0
16	75.0
17	141.0
18	258.0
19	454.0
20	694.0
21	1026.0
22	1524.0
23	2305.0
24	3102.0
25	3936.0
26	5073.0
27	6212.0
28	7707.0
29	9592.0
30	12475.0
31	16143.0
32	21243.0
33	29227.0
34	50220.0
35	64446.0
36	58691.0
37	84199.0
38	134148.0
39	174201.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7893346060719	21.05271582231328	24.454623766064444	11.703325805550383
2	29.799834699199106	25.182616641832745	26.757921633451293	18.259627025516856
3	31.581620180666793	25.27108749301546	27.170155056807598	15.97713726951015
4	28.389830508474578	26.64907920469361	25.863900400447008	19.097189886384804
5	24.15399748556528	30.546307506053267	24.612212469733656	20.687482538647792
6	24.069746461165952	35.89937604768113	24.92011431365245	15.110763177500466
7	81.81341963121625	6.0631635313838705	7.460508241758242	4.662908595641647
8	81.9325933600298	5.160411622276029	7.298408688768858	5.608586328925312
9	76.78018485751537	7.163228720432111	9.588319519463587	6.468266902588936
10	40.9189327621531	23.712807319798845	18.622095595082882	16.74616432296517
11	31.680858865710558	26.513026168746507	21.716101694915256	20.090013270627676
12	27.182232492084186	22.46664881728441	29.899073384242875	20.452045306388527
13	23.380896116595267	27.89305503818216	29.201055829763455	19.524993015459117
14	20.0658584000745	29.775970851182716	28.922110728254797	21.236060020487987
15	18.878340938722292	26.76097737008754	32.93938582603837	21.421295865151798
16	19.910306854162787	23.99247997764947	33.60684601415534	22.49036715403241
17	20.618801219966475	26.077656453715775	26.36547774259639	26.938064583721367
18	22.48381914695474	23.843767461352208	30.56624254982306	23.106170841869993
19	23.18241874650773	27.000634429130194	26.178350251443472	23.63859657291861
20	24.348109517601042	25.649998835909855	27.513707161482586	22.488184485006517
21	22.889504563233377	28.806283758614263	26.72707324455206	21.5771384336003
22	21.613661761966846	24.764562767740735	25.97376140808344	27.648014062208976
23	21.465531290743158	27.990693099273606	25.27967265785062	25.26410295213261
24	21.592999161855094	25.245914043583532	29.713692028310675	23.4473947662507
25	20.796470478673868	27.91531826224623	26.9472317936301	24.340979465449806
26	20.028462004097598	27.664456835537344	27.932052058111378	24.375029102253677
27	21.28931714471969	27.914299683367478	26.617794281989198	24.178588889923635
28	19.299887083255726	26.238300894021233	29.801871856956602	24.659940165766436
29	21.013136757310484	25.554688955112688	28.96561859750419	24.46655569007264
30	21.408345362264853	24.990396256286086	28.704862404544606	24.89639597690445
31	24.104087120506613	26.210217219221455	24.531308204507358	25.154387455764578
32	24.130715682622462	25.37309089215869	26.917110961072826	23.579082464146023
33	22.719692913019184	25.075811370832557	26.021343592847828	26.18315212330043
34	21.72585094989756	23.85220711491898	28.82578226857888	25.596159666604585
35	22.323902262991247	23.49235774818402	28.37280569007264	25.810934298752098
36	24.235047262059975	23.983894812814306	28.359855187185694	23.421202737940025
37	22.352713494132985	25.70020022350531	28.0915324082697	23.85555387409201
38	21.53726834606072	26.015232119575337	28.286663019184203	24.160836515179735
39	24.11892926988266	24.13566306574781	28.660481467684857	23.08492619668467
40	24.809089215868877	22.784008893648725	30.108609610728255	22.298292279754143
41	21.777216427640155	23.78236170609052	27.35000698454088	27.090414881728442
42	22.241251862544235	23.895278450363197	28.905231421121254	24.958238265971318
43	22.559048472713727	23.141966613894578	28.49212493015459	25.8068599832371
44	21.101898631029986	23.859191655801826	29.623911575712427	25.414998137455765
45	22.36901075619296	23.541686068169117	27.979925265412554	26.10937791022537
46	24.362660644440307	24.07527588936487	28.172873207301173	23.38919025889365
47	22.288397513503448	24.128678524864966	29.34482096293537	24.238102998696217
48	21.837603604023094	22.660469826783388	29.986525656546842	25.515400912646673
49	21.73138037809648	22.69612008753958	31.22206183646862	24.350437697895323
50	21.581358260383684	22.31138829390948	30.086055364127397	26.02119808157944
51	20.75660039113429	23.696364546470477	27.59330182529335	27.95373323710188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2171.0
1	1826.0
2	1481.0
3	1441.0
4	1401.0
5	1364.0
6	1327.0
7	1863.5
8	2400.0
9	2829.5
10	3259.0
11	3100.0
12	2941.0
13	2657.5
14	2374.0
15	2243.0
16	2112.0
17	2018.5
18	1925.0
19	2062.0
20	2199.0
21	2260.0
22	2321.0
23	2517.5
24	2714.0
25	3143.0
26	4218.5
27	4865.0
28	5754.0
29	6643.0
30	7843.0
31	9043.0
32	11045.5
33	13048.0
34	13488.0
35	13928.0
36	15371.0
37	16814.0
38	18390.5
39	19967.0
40	22733.5
41	25500.0
42	31767.5
43	38035.0
44	42378.0
45	46721.0
46	52369.0
47	58017.0
48	63139.0
49	68261.0
50	66293.0
51	64325.0
52	57088.5
53	49852.0
54	43914.0
55	37976.0
56	36852.0
57	35728.0
58	32674.0
59	29620.0
60	27752.0
61	25884.0
62	24630.5
63	23377.0
64	20590.0
65	17803.0
66	16023.0
67	14243.0
68	12099.0
69	9955.0
70	9114.5
71	8274.0
72	7514.5
73	6755.0
74	5476.5
75	3498.5
76	2799.0
77	2145.5
78	1492.0
79	1259.0
80	1026.0
81	696.5
82	367.0
83	331.0
84	295.0
85	208.5
86	122.0
87	95.5
88	69.0
89	40.0
90	11.0
91	8.0
92	5.0
93	6.5
94	8.0
95	5.5
96	3.0
97	3.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	687232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.14908159662201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45746311032339	20.959092045699666
2	10.539148350800707	5.933346937711868
3	3.7609722851423375	3.176037472113168
4	1.8451064565920767	2.0775220880425795
5	1.0630037385488793	1.4961289486963327
6	0.7369772923563439	1.244714036243777
7	0.5361127670301365	1.0563757416885486
8	0.40631181560955393	0.9149843561011975
9	0.34652035256950364	0.8778806711432268
>10	5.079964248473598	35.93550650971225
>50	1.153749924517352	20.50867861939027
>100	0.06585449007356388	3.37760807980233
>500	0.006222471503013909	1.19471573609257
>1k	0.002592696459589129	1.2474087575622606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	2405	0.3499546004842615	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	2169	0.31561394114360214	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	1635	0.23791092382194076	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1296	0.18858260383684114	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	1041	0.15147723039672192	No Hit
ACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	853	0.12412111193890855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	825	0.12004679642391508	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	822	0.1196102626187372	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	820	0.11931924008195195	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	753	0.1095699850996461	No Hit
AAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAATTAAT	739	0.10753282734214938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4551126839262434E-4	0.0	0.0	0.24620506612032036	0.0
2	1.4551126839262434E-4	0.0	0.0	1.2211305643509034	0.0
3	1.4551126839262434E-4	0.0	0.0	1.6346735891227417	0.0
4	1.4551126839262434E-4	0.0	0.0	2.143089960886571	0.0
5	1.4551126839262434E-4	0.0	0.0	3.6740140156453718	0.0
6	1.4551126839262434E-4	0.0	0.0	4.704961352207115	0.0
7	1.4551126839262434E-4	0.0	0.0	5.6634440771093315	0.0
8	1.4551126839262434E-4	0.0	0.0	7.126996414602347	0.0
9	1.4551126839262434E-4	0.0	0.0	7.687360309182343	0.0
10	1.4551126839262434E-4	0.0	0.0	8.971060718942075	0.0
11	2.910225367852487E-4	0.0	0.0	10.453675032594525	0.0
12	2.910225367852487E-4	0.0	0.0	11.636536133358167	0.0
13	2.910225367852487E-4	0.0	0.0	12.097806854162787	0.0
14	2.910225367852487E-4	0.0	0.0	12.276058157943751	0.0
15	2.910225367852487E-4	0.0	0.0	12.6124802104675	0.0
16	2.910225367852487E-4	0.0	0.0	13.450479605140622	0.0
17	2.910225367852487E-4	0.0	0.0	14.445049124604209	0.0
18	2.910225367852487E-4	0.0	0.0	15.582219687092568	0.0
19	2.910225367852487E-4	0.0	0.0	16.10344105047495	0.0
20	2.910225367852487E-4	0.0	0.0	16.600944077109332	0.0
21	2.910225367852487E-4	0.0	0.0	17.192301871856955	0.0
22	2.910225367852487E-4	0.0	0.0	17.781476997578693	0.0
23	2.910225367852487E-4	0.0	0.0	18.428711119389085	0.0
24	2.910225367852487E-4	0.0	0.0	18.93567237846899	0.0
25	2.910225367852487E-4	0.0	0.0	19.363621018811696	0.0
26	4.3653380517787296E-4	0.0	0.0	19.781529381635313	0.0
27	4.3653380517787296E-4	0.0	0.0	20.17557389644254	0.0
28	4.3653380517787296E-4	0.0	0.0	20.583150959210283	0.0
29	4.3653380517787296E-4	0.0	0.0	20.999313186813186	0.0
30	4.3653380517787296E-4	0.0	0.0	21.482701620413486	0.0
31	4.3653380517787296E-4	0.0	0.0	21.912832929782084	0.0
32	4.3653380517787296E-4	0.0	0.0	22.322738172844105	0.0
33	4.3653380517787296E-4	0.0	0.0	22.749668234308064	0.0
34	4.3653380517787296E-4	0.0	0.0	23.14967871111939	0.0
35	4.3653380517787296E-4	0.0	0.0	23.600181598062953	0.0
36	4.3653380517787296E-4	0.0	0.0	23.97647373812628	0.0
37	4.3653380517787296E-4	0.0	0.0	24.354512013410318	0.0
38	4.3653380517787296E-4	0.0	0.0	24.743463633823804	0.0
39	4.3653380517787296E-4	0.0	0.0	25.098365617433416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	30	2.1642227E-6	45.000004	1
CGGTCTA	30	2.1642227E-6	45.000004	31
AACCGTT	25	3.8891623E-5	45.0	29
TCGATAG	20	7.0313044E-4	45.0	1
TCTAGCG	25	3.8891623E-5	45.0	1
GCCGATT	45	3.8380676E-10	45.0	9
CCCGTCA	25	3.8891623E-5	45.0	12
CGTAGTT	20	7.0313044E-4	45.0	26
TACGATT	75	0.0	45.0	11
ACCGTTA	25	3.8891623E-5	45.0	30
GATTACG	25	3.8891623E-5	45.0	1
CGTCAAT	25	3.8891623E-5	45.0	14
GTACGAT	75	0.0	45.0	10
GCTACGA	150	0.0	43.5	10
CGAATAT	145	0.0	43.448273	14
CCGATTT	55	6.002665E-11	40.909092	11
GCACTAG	55	6.002665E-11	40.909092	1
AACGGGA	105	0.0	40.714287	4
GACCTAT	150	0.0	40.5	33
AAGCTAC	145	0.0	40.344826	8
>>END_MODULE