FastQCFastQC Report
Sat 18 Jun 2016
SRR3554062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813240
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC26450.32524224091289167No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT16650.20473660911907923No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC13290.16342039250405785No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC12280.15100093453347105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11850.14571344252619153No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC11400.1401800206581083No Hit
TCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC10030.12333382519305494No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8830.108578033544833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAT253.8897953E-545.00000423
AACGAAG253.8897953E-545.00000436
TCGAGTA253.8897953E-545.00000420
ATAACGG502.1827873E-1145.0000042
ATCGAGT253.8897953E-545.00000419
TGCGTAG351.2113378E-745.0000041
AGCGTAA207.0320687E-445.045
ATTCGCC207.0320687E-445.011
TAAGTCG207.0320687E-445.01
TCTAGCG207.0320687E-445.01
CCGTATT207.0320687E-445.016
GTATGCG453.8380676E-1045.01
GTGTCGC207.0320687E-445.014
GTCGTAC207.0320687E-445.014
CTCGCTA207.0320687E-445.039
ACTCGAC207.0320687E-445.011
TATAGCG207.0320687E-445.01
TCGACCA207.0320687E-445.013
TAATGCG207.0320687E-445.01
GTCGGAC207.0320687E-445.09