##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813240
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97604643155772	33.0	31.0	34.0	30.0	34.0
2	32.08469086616497	33.0	31.0	34.0	30.0	34.0
3	32.102614234420344	34.0	31.0	34.0	30.0	34.0
4	35.695718361123404	37.0	35.0	37.0	33.0	37.0
5	35.583641975308645	37.0	35.0	37.0	33.0	37.0
6	35.57380355122719	37.0	35.0	37.0	33.0	37.0
7	35.80882396340564	37.0	35.0	37.0	35.0	37.0
8	35.663319561261126	37.0	35.0	37.0	35.0	37.0
9	37.36557842703261	39.0	37.0	39.0	35.0	39.0
10	36.88058014854163	39.0	37.0	39.0	32.0	39.0
11	36.87194432147951	39.0	37.0	39.0	33.0	39.0
12	36.70569819487482	39.0	35.0	39.0	33.0	39.0
13	36.574232698834294	39.0	35.0	39.0	32.0	39.0
14	37.65336800944371	40.0	36.0	41.0	33.0	41.0
15	37.8188788057646	40.0	36.0	41.0	33.0	41.0
16	37.915505877723675	40.0	36.0	41.0	33.0	41.0
17	37.85324504451331	40.0	36.0	41.0	33.0	41.0
18	37.823615414883676	40.0	36.0	41.0	33.0	41.0
19	37.780601052579804	40.0	36.0	41.0	33.0	41.0
20	37.728459003492205	39.0	36.0	41.0	33.0	41.0
21	37.592574147853036	39.0	35.0	41.0	33.0	41.0
22	37.57622964930402	39.0	35.0	41.0	33.0	41.0
23	37.53617382322562	39.0	35.0	41.0	33.0	41.0
24	37.51653140524323	39.0	35.0	41.0	33.0	41.0
25	37.42107741872018	39.0	35.0	41.0	33.0	41.0
26	37.40315896906203	39.0	35.0	41.0	33.0	41.0
27	37.35973882248783	39.0	35.0	41.0	33.0	41.0
28	37.24286065614087	39.0	35.0	41.0	32.0	41.0
29	37.19746692243372	39.0	35.0	41.0	32.0	41.0
30	37.05989744724805	39.0	35.0	41.0	32.0	41.0
31	36.95710491367862	39.0	35.0	41.0	31.0	41.0
32	36.84934459692096	39.0	35.0	41.0	31.0	41.0
33	36.74848384240814	39.0	35.0	41.0	31.0	41.0
34	36.650628350794356	39.0	35.0	41.0	31.0	41.0
35	36.6078648369485	39.0	35.0	41.0	31.0	41.0
36	36.47335718852983	39.0	35.0	41.0	30.0	41.0
37	36.409303526634204	39.0	35.0	40.0	30.0	41.0
38	36.306632728345875	38.0	35.0	40.0	30.0	41.0
39	36.213933156263835	38.0	35.0	40.0	30.0	41.0
40	36.09549579459938	38.0	35.0	40.0	30.0	41.0
41	35.99006320397422	38.0	35.0	40.0	30.0	41.0
42	35.93806133490728	38.0	35.0	40.0	30.0	41.0
43	35.82424376567803	38.0	35.0	40.0	29.0	41.0
44	35.6807522994442	38.0	34.0	40.0	29.0	41.0
45	35.58746126604692	38.0	34.0	40.0	28.0	41.0
46	35.408585411440654	38.0	34.0	40.0	28.0	41.0
47	35.321400816487134	37.0	34.0	40.0	28.0	41.0
48	35.25794230485466	37.0	34.0	40.0	28.0	41.0
49	35.15584698244061	37.0	34.0	40.0	27.0	41.0
50	35.02580787959274	37.0	34.0	40.0	27.0	41.0
51	33.506822094338695	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	10.0
12	6.0
13	19.0
14	18.0
15	43.0
16	91.0
17	171.0
18	310.0
19	545.0
20	916.0
21	1414.0
22	1983.0
23	2956.0
24	4046.0
25	5370.0
26	6624.0
27	8348.0
28	10564.0
29	12539.0
30	15675.0
31	19937.0
32	25909.0
33	35787.0
34	62001.0
35	78229.0
36	68718.0
37	99756.0
38	156291.0
39	194893.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58253406128572	20.44673159214992	24.003738133884216	10.966996212680144
2	30.974251143573856	25.220599085140915	25.22797698096503	18.5771727903202
3	32.219639958683786	24.600978800846	26.268137327234275	16.911243913235946
4	27.956937681373272	27.340514485268802	24.76390733362845	19.938640499729477
5	23.814618070926173	31.450248389159412	24.316069057104915	20.419064482809503
6	23.98332595543751	37.12188283901431	24.278318823471544	14.616472382076632
7	81.22915744429689	6.738355221090944	7.293172987064089	4.7393143475480795
8	82.56246618464414	5.437263292508977	6.8148394078008945	5.185431115045989
9	76.79774728247504	7.506886036102503	9.657911563622056	6.037455117800404
10	40.76115291918745	24.917859426491564	17.88389651271457	16.437091141606412
11	32.35613103142983	27.437164920564655	21.53792238453593	18.668781663469577
12	28.77920417097044	22.518321774629875	28.59328119620284	20.10919285819684
13	24.083419408784614	28.764817274113426	28.178766415818206	18.972996901283754
14	20.376026757168855	29.966184644139492	28.521838571639368	21.135950027052285
15	19.538143721410652	27.474915154198023	33.044980571541	19.941960552850325
16	22.286532880822392	23.42297476759628	32.604643155771974	21.685849195809357
17	21.0171659042841	25.070090010329054	26.89550440214451	27.017239683242337
18	23.066130539570114	24.139122522256653	29.857237715803453	22.93750922236978
19	25.46161034872854	27.470488416703553	25.120013772072202	21.947887462495697
20	26.46205302247799	26.158821504107028	25.905882642270424	21.473242831144557
21	24.76784221140131	28.598568688210126	26.241208007476267	20.392381092912302
22	22.426712901480496	25.007377895824114	26.04999754070139	26.515911661994
23	21.4691849884413	29.2753676651419	25.251709212532585	24.003738133884216
24	22.494958437853523	25.41660518420147	29.344596920958143	22.743839456986866
25	21.737740396438934	27.101962520289213	27.062121882839012	24.098175200432838
26	21.01200137720722	28.076213663863065	26.234321971373763	24.67746298755595
27	22.954355417834833	27.990507107372974	25.104151296050365	23.95098617874182
28	19.789852933943237	28.03711081599528	28.70739265161576	23.465643598445723
29	22.530987162461265	27.097535782794747	26.613914711524274	23.757562343219714
30	23.871181938911022	26.661871034380997	26.26211204564458	23.204834981063403
31	25.68417687275589	28.8629432885741	22.002360926663716	23.450518912006295
32	24.95819192366337	27.35514731198662	23.8827406423688	23.80392012198121
33	25.169199744232944	27.651123899463876	23.093551719049728	24.086124637253455
34	20.935640155427674	29.20773695342088	25.49075303723378	24.365869853917662
35	22.937878117160988	26.906448280950272	24.477890905513746	25.677782696374997
36	24.011238994638727	28.50609906054793	24.779032020067877	22.703629924745464
37	22.97550538586395	30.537479710786485	24.968275047956322	21.518739855393243
38	22.402980669912942	30.018075844769072	24.28385224533963	23.29509123997836
39	22.7927794992868	27.56959815060745	25.233879297624316	24.403743052481435
40	23.914465594412473	25.40836653386454	26.747823520731888	23.929344350991098
41	20.12112045644582	26.89218434902366	25.525552112537504	27.46114308199302
42	22.42486842752447	25.124563474497073	26.52464217205253	25.925925925925924
43	23.26853081501156	24.806699129408294	27.253332349613892	24.67143770596626
44	22.808396045447836	25.49124489695539	26.330972406669616	25.369386650927154
45	22.71063892577837	26.735772957552506	25.10857803354483	25.44501008312429
46	23.71268014362304	27.010230682209436	25.38266686341056	23.894422310756973
47	21.293468102897055	26.769342383552207	26.8019280901087	25.135261423442035
48	21.570754020953224	25.00282819339924	27.744454281638873	25.68196350400866
49	22.651123899463872	25.27728591805617	28.481629039397966	23.589961143081993
50	21.899562244847772	24.993605823619102	27.945378977915496	25.16145295361763
51	21.057498401455906	26.0105257980424	24.988441296542227	27.943534503959473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2254.0
1	2088.0
2	1922.0
3	1992.0
4	2062.0
5	1945.5
6	1829.0
7	2372.5
8	2916.0
9	3341.0
10	3766.0
11	3743.0
12	3720.0
13	3373.0
14	3026.0
15	2924.0
16	2822.0
17	2762.0
18	2702.0
19	2701.0
20	2700.0
21	2738.0
22	2776.0
23	2939.5
24	3103.0
25	3513.0
26	4567.5
27	5212.0
28	6327.0
29	7442.0
30	8791.0
31	10140.0
32	11413.5
33	12687.0
34	14917.5
35	17148.0
36	18328.5
37	19509.0
38	21667.0
39	23825.0
40	27379.5
41	30934.0
42	36684.5
43	42435.0
44	48650.5
45	54866.0
46	60865.0
47	66864.0
48	73004.5
49	79145.0
50	77730.5
51	76316.0
52	68298.0
53	60280.0
54	53283.5
55	46287.0
56	43214.5
57	40142.0
58	38532.5
59	36923.0
60	35470.5
61	34018.0
62	31119.0
63	28220.0
64	25114.5
65	22009.0
66	19060.0
67	16111.0
68	14172.5
69	12234.0
70	10815.5
71	9397.0
72	8504.0
73	7611.0
74	6397.5
75	4501.0
76	3818.0
77	3082.0
78	2346.0
79	1779.5
80	1213.0
81	907.0
82	601.0
83	496.0
84	391.0
85	309.5
86	228.0
87	163.5
88	99.0
89	64.5
90	30.0
91	25.5
92	21.0
93	27.0
94	33.0
95	16.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	813240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.98194855457504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91678566493874	20.21400856685502
2	10.400753987574118	5.612652180430321
3	3.8944216657314894	3.152372551037684
4	1.9272244528935736	2.0800108416437375
5	1.1053817127913959	1.4912676253852741
6	0.7357336971558345	1.1910917259915612
7	0.4947978035451245	0.9345426216119893
8	0.4246003808742031	0.9165236506400573
9	0.29727085490985644	0.721885222255706
>10	4.121957506851503	27.7166289166221
>50	1.53644421986879	28.079483740864486
>100	0.13684742976819872	5.7876987956811465
>500	0.004576837115993267	0.8428327881136797
>1k	0.0032037859811952876	1.2590007728672474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	2645	0.32524224091289167	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	1665	0.20473660911907923	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	1329	0.16342039250405785	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1228	0.15100093453347105	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1185	0.14571344252619153	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1140	0.1401800206581083	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	1003	0.12333382519305494	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	883	0.108578033544833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.918597216073976E-4	0.0	0.0	0.21445083862082534	0.0
2	4.918597216073976E-4	0.0	0.0	0.9526093158231272	0.0
3	4.918597216073976E-4	0.0	0.0	1.2815405046480743	0.0
4	4.918597216073976E-4	0.0	0.0	1.7361418523437115	0.0
5	4.918597216073976E-4	0.0	0.0	3.009443706654862	0.0
6	4.918597216073976E-4	0.0	0.0	3.9447149672913286	0.0
7	4.918597216073976E-4	0.0	0.0	4.75333234961389	0.0
8	4.918597216073976E-4	0.0	0.0	6.175667699572082	0.0
9	4.918597216073976E-4	0.0	0.0	6.717697112783434	0.0
10	4.918597216073976E-4	0.0	0.0	7.863607299198269	0.0
11	4.918597216073976E-4	0.0	0.0	9.208597707933697	0.0
12	4.918597216073976E-4	0.0	0.0	10.249003984063744	0.0
13	4.918597216073976E-4	0.0	0.0	10.71602478972997	0.0
14	4.918597216073976E-4	0.0	0.0	10.902439624219173	0.0
15	4.918597216073976E-4	0.0	0.0	11.215262407161477	0.0
16	4.918597216073976E-4	0.0	0.0	11.963012148935123	0.0
17	4.918597216073976E-4	0.0	0.0	12.88229796861935	0.0
18	4.918597216073976E-4	0.0	0.0	13.927008017313462	0.0
19	4.918597216073976E-4	0.0	0.0	14.391815454232454	0.0
20	6.14824652009247E-4	0.0	0.0	14.860188874133097	0.0
21	6.14824652009247E-4	0.0	0.0	15.419310412670306	0.0
22	6.14824652009247E-4	0.0	0.0	15.980891249815553	0.0
23	6.14824652009247E-4	0.0	0.0	16.56116275638188	0.0
24	6.14824652009247E-4	0.0	0.0	17.038758546062663	0.0
25	6.14824652009247E-4	0.0	0.0	17.458314888593772	0.0
26	6.14824652009247E-4	0.0	0.0	17.84971226206286	0.0
27	7.377895824110963E-4	0.0	0.0	18.218238158477202	0.0
28	7.377895824110963E-4	0.0	0.0	18.62303870936009	0.0
29	7.377895824110963E-4	0.0	0.0	19.028700014755792	0.0
30	7.377895824110963E-4	0.0	0.0	19.479243519748167	0.0
31	7.377895824110963E-4	0.0	0.0	19.905685898381783	0.0
32	7.377895824110963E-4	0.0	0.0	20.318110274949586	0.0
33	7.377895824110963E-4	0.0	0.0	20.739757021297525	0.0
34	7.377895824110963E-4	0.0	0.0	21.13152328955782	0.0
35	7.377895824110963E-4	0.0	0.0	21.566942108110766	0.0
36	7.377895824110963E-4	0.0	0.0	21.96215139442231	0.0
37	7.377895824110963E-4	0.0	0.0	22.366706015444397	0.0
38	7.377895824110963E-4	0.0	0.0	22.750848458019775	0.0
39	8.607545128129458E-4	0.0	0.0	23.158969062023512	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTAT	25	3.8897953E-5	45.000004	23
AACGAAG	25	3.8897953E-5	45.000004	36
TCGAGTA	25	3.8897953E-5	45.000004	20
ATAACGG	50	2.1827873E-11	45.000004	2
ATCGAGT	25	3.8897953E-5	45.000004	19
TGCGTAG	35	1.2113378E-7	45.000004	1
AGCGTAA	20	7.0320687E-4	45.0	45
ATTCGCC	20	7.0320687E-4	45.0	11
TAAGTCG	20	7.0320687E-4	45.0	1
TCTAGCG	20	7.0320687E-4	45.0	1
CCGTATT	20	7.0320687E-4	45.0	16
GTATGCG	45	3.8380676E-10	45.0	1
GTGTCGC	20	7.0320687E-4	45.0	14
GTCGTAC	20	7.0320687E-4	45.0	14
CTCGCTA	20	7.0320687E-4	45.0	39
ACTCGAC	20	7.0320687E-4	45.0	11
TATAGCG	20	7.0320687E-4	45.0	1
TCGACCA	20	7.0320687E-4	45.0	13
TAATGCG	20	7.0320687E-4	45.0	1
GTCGGAC	20	7.0320687E-4	45.0	9
>>END_MODULE