FastQCFastQC Report
Sat 18 Jun 2016
SRR3554061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143374
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC39430.3448565386304044TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC33700.294741703064789TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT24210.2117417397981763Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG18800.16442563850498612No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC18370.16066483932641462No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC17750.15524229167359063No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16140.14116115986545086No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC14550.12725494894933767TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC13520.11824652300996875No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC11690.10224126138953658No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT253.890792E-545.00000418
TATTACG253.890792E-545.00000437
CGTAGTC207.0332724E-445.018
CCGCGTA351.2119199E-745.01
TAATGCG551.8189894E-1245.01
ATCGATC207.0332724E-445.038
CGTAAGG1550.042.096772
CGCATCG650.041.5384621
TCGTTTA603.6379788E-1241.25000438
ATTAGCG501.0822987E-940.5000041
GTTAACG501.0822987E-940.5000041
TCGGAAG900.040.01
CGTATGG1300.039.807692
TAGAACG403.458208E-739.3751
TCTAGCG800.039.3751
CGTTAGG1600.039.3752
ACGCATT1100.038.86363617
TACGCAT1100.038.86363616
CATACGA1050.038.5714318
AGGGTAC6100.037.9918026