Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554061_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1143374 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC | 3943 | 0.3448565386304044 | TruSeq Adapter, Index 14 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC | 3370 | 0.294741703064789 | TruSeq Adapter, Index 14 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT | 2421 | 0.2117417397981763 | Illumina Paired End PCR Primer 2 (95% over 21bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG | 1880 | 0.16442563850498612 | No Hit |
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC | 1837 | 0.16066483932641462 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC | 1775 | 0.15524229167359063 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 1614 | 0.14116115986545086 | No Hit |
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC | 1455 | 0.12725494894933767 | TruSeq Adapter, Index 14 (95% over 21bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC | 1352 | 0.11824652300996875 | No Hit |
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC | 1169 | 0.10224126138953658 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACACGAT | 25 | 3.890792E-5 | 45.000004 | 18 |
TATTACG | 25 | 3.890792E-5 | 45.000004 | 37 |
CGTAGTC | 20 | 7.0332724E-4 | 45.0 | 18 |
CCGCGTA | 35 | 1.2119199E-7 | 45.0 | 1 |
TAATGCG | 55 | 1.8189894E-12 | 45.0 | 1 |
ATCGATC | 20 | 7.0332724E-4 | 45.0 | 38 |
CGTAAGG | 155 | 0.0 | 42.09677 | 2 |
CGCATCG | 65 | 0.0 | 41.53846 | 21 |
TCGTTTA | 60 | 3.6379788E-12 | 41.250004 | 38 |
ATTAGCG | 50 | 1.0822987E-9 | 40.500004 | 1 |
GTTAACG | 50 | 1.0822987E-9 | 40.500004 | 1 |
TCGGAAG | 90 | 0.0 | 40.0 | 1 |
CGTATGG | 130 | 0.0 | 39.80769 | 2 |
TAGAACG | 40 | 3.458208E-7 | 39.375 | 1 |
TCTAGCG | 80 | 0.0 | 39.375 | 1 |
CGTTAGG | 160 | 0.0 | 39.375 | 2 |
ACGCATT | 110 | 0.0 | 38.863636 | 17 |
TACGCAT | 110 | 0.0 | 38.863636 | 16 |
CATACGA | 105 | 0.0 | 38.57143 | 18 |
AGGGTAC | 610 | 0.0 | 37.991802 | 6 |