##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554061_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143374
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93058789162601	33.0	31.0	34.0	30.0	34.0
2	32.04461707192922	33.0	31.0	34.0	30.0	34.0
3	32.06931240346553	33.0	31.0	34.0	30.0	34.0
4	35.661795702893365	37.0	35.0	37.0	33.0	37.0
5	35.5354958220145	37.0	35.0	37.0	33.0	37.0
6	35.51817515528602	37.0	35.0	37.0	33.0	37.0
7	35.76471215892612	37.0	35.0	37.0	35.0	37.0
8	35.57723894368772	37.0	35.0	37.0	35.0	37.0
9	37.2839062284082	39.0	37.0	39.0	34.0	39.0
10	36.81984809869736	39.0	37.0	39.0	32.0	39.0
11	36.8148278690962	39.0	37.0	39.0	33.0	39.0
12	36.711327177284076	39.0	35.0	39.0	33.0	39.0
13	36.660585250320544	39.0	35.0	39.0	33.0	39.0
14	37.73835945193786	40.0	36.0	41.0	33.0	41.0
15	37.85270523905564	40.0	36.0	41.0	33.0	41.0
16	37.928249199299614	40.0	36.0	41.0	33.0	41.0
17	37.89048290410662	40.0	36.0	41.0	33.0	41.0
18	37.869127687003555	40.0	36.0	41.0	33.0	41.0
19	37.83999986006329	40.0	36.0	41.0	33.0	41.0
20	37.767592231413346	40.0	36.0	41.0	33.0	41.0
21	37.651391408235625	39.0	36.0	41.0	33.0	41.0
22	37.61554486983262	39.0	36.0	41.0	33.0	41.0
23	37.57586931310315	39.0	36.0	41.0	33.0	41.0
24	37.55402431750241	39.0	36.0	41.0	33.0	41.0
25	37.437238383940866	39.0	35.0	41.0	32.0	41.0
26	37.4032731197316	39.0	35.0	41.0	32.0	41.0
27	37.3389267203907	39.0	35.0	41.0	32.0	41.0
28	37.2490226295158	39.0	35.0	41.0	32.0	41.0
29	37.22523426280465	39.0	35.0	41.0	32.0	41.0
30	37.11493527052391	39.0	35.0	41.0	31.0	41.0
31	37.04833763930262	39.0	35.0	41.0	31.0	41.0
32	36.91963347076285	39.0	35.0	41.0	31.0	41.0
33	36.89583198498479	39.0	35.0	41.0	31.0	41.0
34	36.82212994173385	39.0	35.0	41.0	31.0	41.0
35	36.7276988981733	39.0	35.0	41.0	31.0	41.0
36	36.6422745313432	39.0	35.0	41.0	31.0	41.0
37	36.52448105344358	39.0	35.0	40.0	30.0	41.0
38	36.39159715018883	39.0	35.0	40.0	30.0	41.0
39	36.373700993725585	38.0	35.0	40.0	30.0	41.0
40	36.20839637773817	38.0	35.0	40.0	30.0	41.0
41	36.10039234756081	38.0	35.0	40.0	30.0	41.0
42	35.992129434463266	38.0	35.0	40.0	30.0	41.0
43	35.914239785057205	38.0	35.0	40.0	30.0	41.0
44	35.81343287498229	38.0	35.0	40.0	29.0	41.0
45	35.75503116215692	38.0	35.0	40.0	29.0	41.0
46	35.619528693148524	38.0	34.0	40.0	29.0	41.0
47	35.496886408121924	37.0	34.0	40.0	28.0	41.0
48	35.403236386344275	37.0	34.0	40.0	28.0	41.0
49	35.3319307593141	37.0	34.0	40.0	28.0	41.0
50	35.21253413143906	37.0	34.0	40.0	27.0	41.0
51	33.725682934892696	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	6.0
12	6.0
13	9.0
14	20.0
15	55.0
16	118.0
17	216.0
18	377.0
19	693.0
20	1195.0
21	1810.0
22	2657.0
23	3886.0
24	5333.0
25	6954.0
26	8913.0
27	10981.0
28	13788.0
29	17334.0
30	22014.0
31	28546.0
32	37230.0
33	50251.0
34	81963.0
35	102685.0
36	102054.0
37	146986.0
38	227352.0
39	269857.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.861938438341255	20.292747604895688	23.908274982639103	11.937038974123952
2	30.774269836466456	25.25612791614992	25.53757563142069	18.432026615962933
3	31.50902504342411	25.011763429988786	26.64569948240908	16.83351204417802
4	28.317418447507116	26.513459288036984	25.43927009010175	19.72985217435415
5	24.97669179113746	31.038138002088555	23.79746259754026	20.187707609233723
6	24.623876351919844	36.19078271851555	23.890258130760365	15.295082798804241
7	81.14142878883025	6.1488191965183745	7.828759443541658	4.880992571109716
8	81.66470463732777	5.803787736995943	7.13108746569364	5.400420159982648
9	76.01852062404777	7.032519543036661	9.859853381308303	7.08910645160726
10	40.64671752200068	23.291941219583443	18.61464402723868	17.4466972311772
11	30.396790551473096	26.853680423028685	21.79470584428192	20.954823181216295
12	25.74573149293232	22.72388562272712	30.132572544066942	21.39781034027361
13	23.81845310458345	25.96289578038332	29.82147573759767	20.397175377435556
14	20.83194125456762	27.799477686216406	29.21817358099799	22.150407478217975
15	20.187707609233723	26.700537182059414	31.76782050317744	21.343934705529424
16	21.82881541822711	23.385348975925638	31.03665117450633	23.749184431340925
17	20.81558615116314	25.0809446427853	27.650182704871725	26.45328650117984
18	22.95425643752613	23.86856794014907	29.347964882881715	23.829210739443088
19	23.63898426936418	27.13530305919148	25.31035339267816	23.915359278766175
20	24.517087147337616	25.997530117004587	26.054554327805253	23.430828407852548
21	24.400852214585953	27.174485339005432	26.290522611149107	22.134139835259504
22	22.248013335968807	24.122465614925652	26.322708055282	27.30681299382354
23	21.730859718692223	26.629956602126686	25.78823726969478	25.85094640948631
24	22.39827038221964	24.62518825861004	28.46085357896891	24.515687780201404
25	22.457830945954694	26.389003073360072	26.14630033567319	25.006865645012045
26	20.90470834565068	25.878146608196445	26.726600394971374	26.4905446511815
27	22.80303732636915	25.434809607355074	25.929310969114216	25.832842097161556
28	20.120188144911463	26.58753828581024	27.663389232219725	25.628884337058565
29	21.82592922350867	25.38189603751703	26.015022206207245	26.777152532767058
30	23.044777999149886	24.51796175179775	26.614651024074366	25.822609224978006
31	24.484376940528644	26.10668075362917	21.963854346871628	27.445087958970554
32	22.827788632590913	26.425211698009576	24.501956490177317	26.2450431792222
33	22.49202798034589	24.959899385502908	24.782791982325993	27.765280651825215
34	21.572818692746207	24.951328261793606	26.578092557640808	26.897760487819383
35	21.909016647221293	24.530031293347584	26.300143260210568	27.260808799220555
36	23.487152935085106	24.26388915612914	27.48243356941823	24.76652433936752
37	22.838458807004532	26.104406782032825	27.15821769604696	23.89891671491568
38	21.385216036047698	26.80811353065576	27.66268954865162	24.143980884644918
39	23.68971132805189	23.98768906761917	27.363487362840154	24.959112241488786
40	23.396893754799393	22.218714086554357	30.533403768145856	23.850988390500397
41	21.236533277825103	23.200020290823474	27.96775158434598	27.595694847005444
42	21.731297020922288	23.512778845767002	28.561608012776222	26.194316120534488
43	22.631877233521138	22.658377748663167	28.119407997733028	26.590337020082664
44	21.93630430637744	23.74953427312498	27.541644291369227	26.772517129128353
45	23.11955668049125	22.939213240811842	26.80067939274463	27.14055068595228
46	23.988388751187276	22.99956094856101	27.76571795405528	25.246332346196432
47	22.07982689828525	23.890870353882455	29.08864466045231	24.940658087379983
48	21.063711436502842	23.5555470038675	28.54096734751709	26.839774212112573
49	21.749138951909	23.643794593894913	29.523410537584375	25.083655916611715
50	21.525502591453016	22.100117721760334	30.146741136321097	26.227638550465553
51	21.279388896371614	23.060783260770318	27.347569561665736	28.31225828119233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1930.0
1	1797.0
2	1664.0
3	1938.5
4	2213.0
5	1986.0
6	1759.0
7	2274.0
8	2789.0
9	3197.5
10	3606.0
11	3592.0
12	3578.0
13	3392.0
14	3206.0
15	2943.0
16	2680.0
17	2600.5
18	2521.0
19	2672.5
20	2824.0
21	2943.0
22	3062.0
23	3244.0
24	3426.0
25	4503.0
26	6310.5
27	7041.0
28	9022.0
29	11003.0
30	13082.0
31	15161.0
32	16398.5
33	17636.0
34	20643.5
35	23651.0
36	25164.0
37	26677.0
38	27806.0
39	28935.0
40	34694.5
41	40454.0
42	47300.5
43	54147.0
44	61326.0
45	68505.0
46	82551.0
47	96597.0
48	106011.0
49	115425.0
50	112975.0
51	110525.0
52	97257.0
53	83989.0
54	76131.0
55	68273.0
56	64920.0
57	61567.0
58	57796.0
59	54025.0
60	51891.0
61	49757.0
62	46348.0
63	42939.0
64	38793.5
65	34648.0
66	30551.0
67	26454.0
68	22815.5
69	19177.0
70	17077.0
71	14977.0
72	13436.0
73	11895.0
74	10081.5
75	6627.5
76	4987.0
77	3743.5
78	2500.0
79	2030.5
80	1561.0
81	1253.5
82	946.0
83	666.5
84	387.0
85	287.5
86	188.0
87	141.5
88	95.0
89	83.0
90	71.0
91	47.5
92	24.0
93	28.0
94	32.0
95	25.5
96	19.0
97	9.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1143374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.064180634532075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53379550633716	19.16598128819915
2	10.830891251297725	5.645966120135941
3	3.9986740333206425	3.126664869092465
4	2.0176266942430776	2.103511464472216
5	1.1652494848789652	1.51856365290904
6	0.7952480930777057	1.24364939683467
7	0.5608031541638318	1.0231812297379015
8	0.4364584623545248	0.9100745761822762
9	0.3601891067557375	0.8449230546965071
>10	4.683950306679969	30.98237916599096
>50	1.5136213409413997	25.99497868926518
>100	0.09236795788303924	4.129469217069461
>500	0.007416405377467206	1.3882636203600367
>1k	0.003708202688733603	1.9223936550542557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3943	0.3448565386304044	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3370	0.294741703064789	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	2421	0.2117417397981763	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	1880	0.16442563850498612	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1837	0.16066483932641462	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	1775	0.15524229167359063	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1614	0.14116115986545086	No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1455	0.12725494894933767	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	1352	0.11824652300996875	No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1169	0.10224126138953658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4984178405316195E-4	0.0	0.0	0.21961317993937243	0.0
2	3.4984178405316195E-4	0.0	0.0	1.1268403864352348	0.0
3	3.4984178405316195E-4	0.0	0.0	1.5022206207242774	0.0
4	3.4984178405316195E-4	0.0	0.0	2.0151761365922263	0.0
5	3.4984178405316195E-4	0.0	0.0	3.6182386515698277	0.0
6	3.4984178405316195E-4	0.0	0.0	4.699687066524165	0.0
7	3.4984178405316195E-4	0.0	0.0	5.7113420455598956	0.0
8	3.4984178405316195E-4	0.0	0.0	7.284405627554938	0.0
9	3.4984178405316195E-4	0.0	0.0	7.87240220610229	0.0
10	3.4984178405316195E-4	0.0	0.0	9.196028596067428	0.0
11	3.4984178405316195E-4	0.0	0.0	10.568457914907983	0.0
12	3.4984178405316195E-4	0.0	0.0	11.73867868256581	0.0
13	3.4984178405316195E-4	0.0	0.0	12.18175330206914	0.0
14	4.3730223006645247E-4	0.0	0.0	12.354750064283428	0.0
15	4.3730223006645247E-4	0.0	0.0	12.697857393993566	0.0
16	4.3730223006645247E-4	0.0	0.0	13.469433448722816	0.0
17	4.3730223006645247E-4	0.0	0.0	14.389779722120672	0.0
18	4.3730223006645247E-4	0.0	0.0	15.401172319818363	0.0
19	4.3730223006645247E-4	0.0	0.0	15.923223722071693	0.0
20	4.3730223006645247E-4	0.0	0.0	16.394635526083327	0.0
21	4.3730223006645247E-4	0.0	0.0	16.94012632786822	0.0
22	4.3730223006645247E-4	0.0	0.0	17.57587630993883	0.0
23	4.3730223006645247E-4	0.0	0.0	18.193172137900635	0.0
24	5.24762676079743E-4	0.0	0.0	18.710588136515263	0.0
25	6.122231220930334E-4	0.0	0.0	19.154537360478724	0.0
26	6.122231220930334E-4	0.0	0.0	19.55099556225697	0.0
27	6.122231220930334E-4	0.0	0.0	19.955412664622425	0.0
28	6.122231220930334E-4	0.0	0.0	20.395601089407315	0.0
29	6.122231220930334E-4	0.0	0.0	20.81943441078772	0.0
30	6.122231220930334E-4	0.0	0.0	21.286910494728758	0.0
31	6.996835681063239E-4	0.0	0.0	21.69071537397212	0.0
32	6.996835681063239E-4	0.0	0.0	22.115860602042726	0.0
33	6.996835681063239E-4	0.0	0.0	22.542755039033597	0.0
34	6.996835681063239E-4	0.0	0.0	22.945422932478788	0.0
35	6.996835681063239E-4	0.0	0.0	23.412024411959692	0.0
36	7.871440141196144E-4	0.0	0.0	23.81574183075704	0.0
37	7.871440141196144E-4	0.0	0.0	24.22951720084592	0.0
38	7.871440141196144E-4	0.0	0.0	24.588542331730476	0.0
39	7.871440141196144E-4	0.0	0.0	24.97782877693563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	25	3.890792E-5	45.000004	18
TATTACG	25	3.890792E-5	45.000004	37
CGTAGTC	20	7.0332724E-4	45.0	18
CCGCGTA	35	1.2119199E-7	45.0	1
TAATGCG	55	1.8189894E-12	45.0	1
ATCGATC	20	7.0332724E-4	45.0	38
CGTAAGG	155	0.0	42.09677	2
CGCATCG	65	0.0	41.53846	21
TCGTTTA	60	3.6379788E-12	41.250004	38
ATTAGCG	50	1.0822987E-9	40.500004	1
GTTAACG	50	1.0822987E-9	40.500004	1
TCGGAAG	90	0.0	40.0	1
CGTATGG	130	0.0	39.80769	2
TAGAACG	40	3.458208E-7	39.375	1
TCTAGCG	80	0.0	39.375	1
CGTTAGG	160	0.0	39.375	2
ACGCATT	110	0.0	38.863636	17
TACGCAT	110	0.0	38.863636	16
CATACGA	105	0.0	38.57143	18
AGGGTAC	610	0.0	37.991802	6
>>END_MODULE