SRR3554060_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471936
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2428	0.5144765391917547	RNA PCR Primer, Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2039	0.4320501084892867	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1618	0.3428430973691348	TruSeq Adapter, Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1198	0.25384797938703557	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	789	0.16718368592351507	No Hit
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	732	0.15510577705451586	TruSeq Adapter, Index 7 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	725	0.15362252508814755	No Hit
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	681	0.14429922701383238	Illumina PCR Primer Index 3 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	656	0.13900189856251696	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	603	0.12777156224572822	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	601	0.12734777596962302	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	542	0.11484608082451858	TruSeq Adapter, Index 7 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATC	25	3.8872982E-5	45.000004	34
TATTTCG	25	3.8872982E-5	45.000004	1
CGGCGTA	25	3.8872982E-5	45.000004	20
CAAGTCA	25	3.8872982E-5	45.000004	24
TTTACGG	25	3.8872982E-5	45.000004	2
GAACTCG	25	3.8872982E-5	45.000004	16
GCGCCTA	25	3.8872982E-5	45.000004	21
ATCGTAA	25	3.8872982E-5	45.000004	19
TACTGGC	25	3.8872982E-5	45.000004	28
GCTTACG	35	1.2099008E-7	45.000004	1
ATAAGCA	25	3.8872982E-5	45.000004	27
CGGTGAT	25	3.8872982E-5	45.000004	36
CATACGA	70	0.0	45.000004	18
CGTACGC	25	3.8872982E-5	45.000004	15
GTATACG	30	2.1627711E-6	45.000004	1
CGGTCTA	25	3.8872982E-5	45.000004	31
GCAATAG	25	3.8872982E-5	45.000004	1
GGTCTAT	30	2.1627711E-6	45.000004	8
CCCGGTG	30	2.1627711E-6	45.000004	27
TCAAGTG	25	3.8872982E-5	45.000004	25