##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471936
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916963317059942	33.0	31.0	34.0	30.0	34.0
2	32.023704485353946	33.0	31.0	34.0	30.0	34.0
3	32.03383721521562	33.0	31.0	34.0	30.0	34.0
4	35.641663276376455	37.0	35.0	37.0	33.0	37.0
5	35.53225649240575	37.0	35.0	37.0	33.0	37.0
6	35.526556566992134	37.0	35.0	37.0	33.0	37.0
7	35.759249135476	37.0	35.0	37.0	35.0	37.0
8	35.60967800718741	37.0	35.0	37.0	35.0	37.0
9	37.31688195009493	39.0	37.0	39.0	34.0	39.0
10	36.81591147952265	39.0	37.0	39.0	32.0	39.0
11	36.843222386086246	39.0	37.0	39.0	33.0	39.0
12	36.65540242744779	39.0	35.0	39.0	33.0	39.0
13	36.48625449213453	39.0	35.0	39.0	32.0	39.0
14	37.52339512137239	39.0	36.0	41.0	33.0	41.0
15	37.73273070924871	39.0	36.0	41.0	33.0	41.0
16	37.853410208163815	40.0	36.0	41.0	33.0	41.0
17	37.811601573094656	39.0	36.0	41.0	33.0	41.0
18	37.78143434703011	39.0	36.0	41.0	33.0	41.0
19	37.72431643273664	39.0	36.0	41.0	33.0	41.0
20	37.66876864659615	39.0	35.0	41.0	33.0	41.0
21	37.522337774613504	39.0	35.0	41.0	33.0	41.0
22	37.514813449281256	39.0	35.0	41.0	33.0	41.0
23	37.456879746406294	39.0	35.0	41.0	33.0	41.0
24	37.46104980336317	39.0	35.0	41.0	33.0	41.0
25	37.359108014646054	39.0	35.0	41.0	33.0	41.0
26	37.33507085706537	39.0	35.0	41.0	33.0	41.0
27	37.2513709486032	39.0	35.0	41.0	33.0	41.0
28	37.19341393748305	39.0	35.0	41.0	32.0	41.0
29	37.15635170870627	39.0	35.0	41.0	32.0	41.0
30	36.98312271155411	39.0	35.0	41.0	32.0	41.0
31	36.87769104285327	39.0	35.0	41.0	31.0	41.0
32	36.76680100691619	39.0	35.0	41.0	31.0	41.0
33	36.65832655275292	39.0	35.0	40.0	31.0	41.0
34	36.59933762205045	39.0	35.0	40.0	31.0	41.0
35	36.5059520782479	39.0	35.0	40.0	31.0	41.0
36	36.40787733930024	38.0	35.0	40.0	31.0	41.0
37	36.269640374966094	38.0	35.0	40.0	30.0	41.0
38	36.19530614320586	38.0	35.0	40.0	30.0	41.0
39	36.11348148901546	38.0	35.0	40.0	30.0	41.0
40	35.989424413479796	38.0	35.0	40.0	30.0	41.0
41	35.87079604014104	38.0	35.0	40.0	29.0	41.0
42	35.81297675956062	38.0	35.0	40.0	29.0	41.0
43	35.65566729387036	38.0	35.0	40.0	29.0	41.0
44	35.50789090046108	38.0	34.0	40.0	29.0	41.0
45	35.467711723623545	38.0	34.0	40.0	28.0	41.0
46	35.33767502373203	37.0	34.0	40.0	28.0	41.0
47	35.24191203892053	37.0	34.0	40.0	28.0	41.0
48	35.22016544616219	37.0	34.0	40.0	28.0	41.0
49	35.134037242337946	37.0	34.0	40.0	27.0	41.0
50	35.03033038378085	37.0	34.0	40.0	27.0	41.0
51	33.44471072348793	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	4.0
13	11.0
14	8.0
15	22.0
16	49.0
17	86.0
18	208.0
19	368.0
20	670.0
21	907.0
22	1345.0
23	1806.0
24	2560.0
25	3204.0
26	3942.0
27	4725.0
28	5656.0
29	7080.0
30	9227.0
31	11681.0
32	15263.0
33	21041.0
34	37057.0
35	50820.0
36	39773.0
37	56515.0
38	88301.0
39	109566.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10846385950637	21.557372186059126	24.13950196636832	12.194661988066178
2	30.39501118795769	25.583341809058858	26.194653512340654	17.8269934906428
3	30.133323162462705	25.705392256577163	26.92441347979387	17.23687110116626
4	27.374898291293736	27.775164429075126	25.943136357472202	18.906800922158936
5	23.029605709248713	30.768366897206402	26.022596284241935	20.179431109302957
6	23.18344012747491	36.36912632221318	25.174599945755354	15.27283360455655
7	79.80171040141036	7.142493897477624	7.868651681583944	5.187144019528072
8	80.26660394629779	5.441627678329265	7.99303295362083	6.298735421752102
9	75.08390968266883	7.6868473691347985	10.810576010306482	6.418666937889883
10	41.175074586384596	21.67433719826417	20.531385611608354	16.61920260374288
11	31.038318755085438	27.703332655275293	22.105963520477353	19.15238506916192
12	26.624796582587468	22.062737320314618	32.51245931651749	18.800006780580418
13	22.783809669107676	30.400732302685107	29.220699416870083	17.59475861133713
14	18.610574315161376	30.427218944941686	31.76723114998644	19.194975589910495
15	16.155792310821806	29.09250406834825	35.49824552481692	19.253458096013016
16	17.918107540005423	23.667615947925142	36.509399579604015	21.90487693246542
17	18.532385747219962	24.97160631950095	28.060160699755897	28.435847233523187
18	20.779936262544073	23.101861269324655	33.03943755085436	23.07876491727692
19	22.458977488473014	28.604090385136967	25.93402495253594	23.00290717385408
20	23.459960672633578	26.274325332248438	27.95993524545701	22.30577874966097
21	21.343148223487933	29.55167649850827	28.19450094928126	20.91067432872254
22	19.87790717385408	25.58058719826417	26.441720911310007	28.09978471657174
23	18.66714978302143	29.61715147816653	25.64436703281801	26.071331705994034
24	20.539013764578247	24.396316449688094	31.370779088689993	23.693890697043667
25	18.280021019799296	28.555143070246814	28.218656767019258	24.946179142934636
26	18.00816212367779	28.587562720368865	29.966139476539194	23.438135679414156
27	19.82832417954977	30.559016476810413	26.880975386493084	22.731683957146732
28	19.475098318416055	26.22177583401139	31.432863778139406	22.870262069433142
29	19.51747694602658	25.663649308380798	30.48337062652563	24.335503119066992
30	20.624618592351503	27.60819263628967	28.888874762679684	22.878314008679144
31	22.91030987252509	30.641654800650937	23.23281122864117	23.215224098182805
32	22.939974911852453	26.88542514239219	27.18504203959859	22.989557906156765
33	22.442661716842963	27.376381543260102	24.323637103336047	25.857319636560888
34	20.15612286411717	25.33860523460808	29.04737082994304	25.457901071331708
35	18.086562584757253	27.031631407648494	28.111650732302685	26.770155275291565
36	23.66592080282072	25.23901545972335	26.77396935177651	24.321094385679416
37	21.910386154054788	28.51594283970708	26.899833875779766	22.67383713045837
38	20.821255424464336	26.430066788717117	26.296786004882016	26.45189178193653
39	21.277249457553566	25.82045023053973	26.415446162191486	26.486854149715217
40	22.374644019528073	23.221368999186332	28.65663988337402	25.747347097911582
41	18.567348114998644	26.185118321128286	25.535242066720908	29.712291497152155
42	22.30726200162734	22.87005017629509	28.269299227013832	26.553388595063737
43	22.651800244100894	23.543870355302413	28.466359845402767	25.337969555193922
44	21.002424057499322	23.528614049362627	29.889434160564143	25.579527732573908
45	21.076798548955793	25.8662191483591	26.994550108489285	26.06243219419582
46	22.746728369948467	25.66788717114185	26.99243117710876	24.592953281800924
47	19.554982031461893	25.34665717385408	28.93167717656631	26.16668361811771
48	20.375008475725522	22.705197314890153	29.45992676973149	27.459867439652836
49	21.208172294548415	21.236989761323567	30.903130933007866	26.651707011120152
50	20.89711316788717	22.350064415513966	30.420226471385952	26.332595945212912
51	19.832773935448873	23.87633068890697	26.655309194467048	29.63558618117711
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1858.0
1	1597.0
2	1336.0
3	1323.0
4	1310.0
5	1323.0
6	1336.0
7	1969.5
8	2603.0
9	2988.5
10	3374.0
11	3246.0
12	3118.0
13	2879.0
14	2640.0
15	2573.0
16	2506.0
17	2334.5
18	2163.0
19	2118.0
20	2073.0
21	2053.0
22	2033.0
23	1878.0
24	1723.0
25	2191.0
26	2775.0
27	2891.0
28	3627.0
29	4363.0
30	4957.0
31	5551.0
32	6221.0
33	6891.0
34	7781.5
35	8672.0
36	9795.0
37	10918.0
38	11693.5
39	12469.0
40	15505.0
41	18541.0
42	23347.0
43	28153.0
44	31691.5
45	35230.0
46	39187.0
47	43144.0
48	47436.5
49	51729.0
50	51261.0
51	50793.0
52	42103.0
53	33413.0
54	29274.0
55	25135.0
56	21909.5
57	18684.0
58	17558.0
59	16432.0
60	15900.0
61	15368.0
62	13740.0
63	12112.0
64	11519.0
65	10926.0
66	9344.0
67	7762.0
68	6986.0
69	6210.0
70	5510.0
71	4810.0
72	4540.5
73	4271.0
74	3419.5
75	2042.0
76	1516.0
77	1199.0
78	882.0
79	878.5
80	875.0
81	725.0
82	575.0
83	391.0
84	207.0
85	126.0
86	45.0
87	35.0
88	25.0
89	15.5
90	6.0
91	4.5
92	3.0
93	5.5
94	8.0
95	12.5
96	17.0
97	12.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	471936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.66458843331938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69432098813245	23.96829520828783
2	10.396492377072688	6.58401304540298
3	3.927497493654915	3.730877751284288
4	1.9244104247042975	2.4374265630020373
5	1.1412037235322994	1.8067873112110926
6	0.7883321887753499	1.4977328583785579
7	0.5602276339740636	1.241756422113271
8	0.40576912261465015	1.0278809813233603
9	0.30150477479298365	0.8592322144050427
>10	3.7304487345337813	28.89710249753385
>50	1.0606489663552017	21.828345374350704
>100	0.06108801008754643	3.4397005078122045
>500	0.005370374513190894	1.132750216668597
>1k	0.002685187256595447	1.548099048226195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2428	0.5144765391917547	RNA PCR Primer, Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2039	0.4320501084892867	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1618	0.3428430973691348	TruSeq Adapter, Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1198	0.25384797938703557	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	789	0.16718368592351507	No Hit
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	732	0.15510577705451586	TruSeq Adapter, Index 7 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	725	0.15362252508814755	No Hit
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	681	0.14429922701383238	Illumina PCR Primer Index 3 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	656	0.13900189856251696	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	603	0.12777156224572822	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	601	0.12734777596962302	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	542	0.11484608082451858	TruSeq Adapter, Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.118931380526173E-4	0.0	0.0	0.34856421209655547	0.0
2	2.118931380526173E-4	0.0	0.0	1.631153376729048	0.0
3	2.118931380526173E-4	0.0	0.0	2.1519867100623813	0.0
4	2.118931380526173E-4	0.0	0.0	2.7560940466503934	0.0
5	2.118931380526173E-4	0.0	0.0	4.591088622186059	0.0
6	2.118931380526173E-4	0.0	0.0	5.873042107404394	0.0
7	2.118931380526173E-4	0.0	0.0	7.09036818551668	0.0
8	2.118931380526173E-4	0.0	0.0	9.098267561703281	0.0
9	2.118931380526173E-4	0.0	0.0	9.883119745050177	0.0
10	2.118931380526173E-4	0.0	0.0	11.567458299430431	0.0
11	2.118931380526173E-4	0.0	0.0	13.692958367236235	0.0
12	2.118931380526173E-4	0.0	0.0	15.259484336859234	0.0
13	2.118931380526173E-4	0.0	0.0	15.850454298887986	0.0
14	2.118931380526173E-4	0.0	0.0	16.10832824789802	0.0
15	2.118931380526173E-4	0.0	0.0	16.539318890697043	0.0
16	2.118931380526173E-4	0.0	0.0	17.71532580688907	0.0
17	2.118931380526173E-4	0.0	0.0	19.126745999457555	0.0
18	2.118931380526173E-4	0.0	0.0	20.712130458367238	0.0
19	2.118931380526173E-4	0.0	0.0	21.38425549227014	0.0
20	2.118931380526173E-4	0.0	0.0	21.99895748576078	0.0
21	2.118931380526173E-4	0.0	0.0	22.69566212367779	0.0
22	2.118931380526173E-4	0.0	0.0	23.424998304854896	0.0
23	2.118931380526173E-4	0.0	0.0	24.16620050176295	0.0
24	2.118931380526173E-4	0.0	0.0	24.73343843232981	0.0
25	2.118931380526173E-4	0.0	0.0	25.213588283157037	0.0
26	2.118931380526173E-4	0.0	0.0	25.668946636832114	0.0
27	2.118931380526173E-4	0.0	0.0	26.094851844317873	0.0
28	2.118931380526173E-4	0.0	0.0	26.55190534309737	0.0
29	2.118931380526173E-4	0.0	0.0	27.002602047735287	0.0
30	2.118931380526173E-4	0.0	0.0	27.553100420395985	0.0
31	2.118931380526173E-4	0.0	0.0	28.03897138595064	0.0
32	2.118931380526173E-4	0.0	0.0	28.492846487659342	0.0
33	2.118931380526173E-4	0.0	0.0	28.92404902359642	0.0
34	2.118931380526173E-4	0.0	0.0	29.343809330078656	0.0
35	2.118931380526173E-4	0.0	0.0	29.853200433957145	0.0
36	2.118931380526173E-4	0.0	0.0	30.27105370219691	0.0
37	2.118931380526173E-4	0.0	0.0	30.69717080282072	0.0
38	2.118931380526173E-4	0.0	0.0	31.088537428803907	0.0
39	2.118931380526173E-4	0.0	0.0	31.48393002441009	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATC	25	3.8872982E-5	45.000004	34
TATTTCG	25	3.8872982E-5	45.000004	1
CGGCGTA	25	3.8872982E-5	45.000004	20
CAAGTCA	25	3.8872982E-5	45.000004	24
TTTACGG	25	3.8872982E-5	45.000004	2
GAACTCG	25	3.8872982E-5	45.000004	16
GCGCCTA	25	3.8872982E-5	45.000004	21
ATCGTAA	25	3.8872982E-5	45.000004	19
TACTGGC	25	3.8872982E-5	45.000004	28
GCTTACG	35	1.2099008E-7	45.000004	1
ATAAGCA	25	3.8872982E-5	45.000004	27
CGGTGAT	25	3.8872982E-5	45.000004	36
CATACGA	70	0.0	45.000004	18
CGTACGC	25	3.8872982E-5	45.000004	15
GTATACG	30	2.1627711E-6	45.000004	1
CGGTCTA	25	3.8872982E-5	45.000004	31
GCAATAG	25	3.8872982E-5	45.000004	1
GGTCTAT	30	2.1627711E-6	45.000004	8
CCCGGTG	30	2.1627711E-6	45.000004	27
TCAAGTG	25	3.8872982E-5	45.000004	25
>>END_MODULE