FastQCFastQC Report
Sat 18 Jun 2016
SRR3554059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485303
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC11680.24067438280826617No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8560.17638465041427726No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC7950.16381518350391405Illumina Single End Adapter 2 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC7410.15268811443572367No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT5860.12074930507332533No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT5580.11497971370463402Illumina Single End Adapter 2 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.10611926981700093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA207.0292526E-445.00000442
CGGTTAG207.0292526E-445.00000443
TAAACGG351.2099918E-745.0000042
GTGATAA207.0292526E-445.0000049
TGTGCGA207.0292526E-445.00000410
TCGATAG302.1629003E-645.0000041
TATGATC207.0292526E-445.00000435
TACGATT302.1629003E-645.00000411
CGCTAGG351.2099918E-745.0000042
TATTACG207.0292526E-445.0000041
TCGTAAG351.2099918E-745.0000041
CGGTCTA600.045.00000431
CGCACGA207.0292526E-445.00000439
CCTATTA207.0292526E-445.00000438
ATTGACG207.0292526E-445.00000440
CGTTTAC302.1629003E-645.00000433
TTTTACG453.8380676E-1045.0000041
CTAATCG207.0292526E-445.00000418
TCACGTC302.1629003E-645.00000438
TAATCGA207.0292526E-445.00000419