FastQCFastQC Report
Sat 18 Jun 2016
SRR3554058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785140
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC14100.17958580635300708No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC10080.1283847466693838No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT8870.11297348243625341No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7890.10049163206561887No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT207.031919E-445.011
GGTACGC253.8896724E-545.08
CACGCGT207.031919E-445.040
CGACAAT406.8102963E-945.020
GTTCGGA207.031919E-445.031
CGGATTC253.8896724E-545.020
TCGCTAA253.8896724E-545.030
CGGTCTA406.8102963E-945.031
ATAACGG207.031919E-445.02
CGATCAC207.031919E-445.029
TCGGTAG207.031919E-445.02
ATCGATC207.031919E-445.017
AATCGAT207.031919E-445.016
TAATCCG253.8896724E-545.016
AACGTAG302.1646192E-644.9999961
TTGCGTC302.1646192E-644.99999620
CGTACGT302.1646192E-644.99999614
CGTGTTA302.1646192E-644.99999641
TACGCAT1300.043.2692316
CGCACGG850.042.352942