##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554058_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785140
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14880403494918	33.0	31.0	34.0	30.0	34.0
2	32.24947270550475	34.0	31.0	34.0	30.0	34.0
3	32.28781236467382	34.0	31.0	34.0	30.0	34.0
4	35.84077489364954	37.0	35.0	37.0	35.0	37.0
5	35.71913926178771	37.0	35.0	37.0	35.0	37.0
6	35.71777390019614	37.0	35.0	37.0	33.0	37.0
7	35.915876149476524	37.0	35.0	37.0	35.0	37.0
8	35.780572891458846	37.0	35.0	37.0	35.0	37.0
9	37.456284229564154	39.0	37.0	39.0	35.0	39.0
10	37.0420205313702	39.0	37.0	39.0	33.0	39.0
11	36.981358738568915	39.0	37.0	39.0	33.0	39.0
12	36.77858343734875	39.0	35.0	39.0	33.0	39.0
13	36.68148737804723	39.0	35.0	39.0	33.0	39.0
14	37.805476730264665	40.0	36.0	41.0	33.0	41.0
15	37.94086787069822	40.0	36.0	41.0	33.0	41.0
16	38.02393203759839	40.0	36.0	41.0	33.0	41.0
17	37.95874621086685	40.0	36.0	41.0	33.0	41.0
18	37.94355528950251	40.0	36.0	41.0	33.0	41.0
19	37.9079820159462	40.0	36.0	41.0	33.0	41.0
20	37.85566777899483	40.0	36.0	41.0	33.0	41.0
21	37.74517283541789	40.0	35.0	41.0	33.0	41.0
22	37.73554652673408	40.0	35.0	41.0	33.0	41.0
23	37.665590850039486	40.0	35.0	41.0	33.0	41.0
24	37.64375398018188	40.0	35.0	41.0	33.0	41.0
25	37.52325445143541	39.0	35.0	41.0	33.0	41.0
26	37.48611330463357	39.0	35.0	41.0	33.0	41.0
27	37.44689864228036	39.0	35.0	41.0	33.0	41.0
28	37.34419976055226	39.0	35.0	41.0	33.0	41.0
29	37.31217999337698	39.0	35.0	41.0	33.0	41.0
30	37.17269404182694	39.0	35.0	41.0	32.0	41.0
31	37.050313319917464	39.0	35.0	41.0	32.0	41.0
32	36.951729627837075	39.0	35.0	41.0	31.0	41.0
33	36.854796596785285	39.0	35.0	41.0	31.0	41.0
34	36.74179509386861	39.0	35.0	41.0	31.0	41.0
35	36.65764322286471	39.0	35.0	41.0	31.0	41.0
36	36.56047838602033	39.0	35.0	41.0	31.0	41.0
37	36.484141681738286	39.0	35.0	41.0	31.0	41.0
38	36.35015156532593	38.0	35.0	41.0	30.0	41.0
39	36.29252362635963	38.0	35.0	41.0	30.0	41.0
40	36.11536413887969	38.0	35.0	40.0	30.0	41.0
41	36.00619252617368	38.0	35.0	40.0	30.0	41.0
42	35.91328425503732	38.0	35.0	40.0	30.0	41.0
43	35.77994370430751	38.0	35.0	40.0	29.0	41.0
44	35.66016761341926	38.0	34.0	40.0	29.0	41.0
45	35.56787961382683	37.0	34.0	40.0	29.0	41.0
46	35.43112438545992	37.0	34.0	40.0	28.0	41.0
47	35.28679853274575	37.0	34.0	40.0	28.0	41.0
48	35.209538426267926	37.0	34.0	40.0	28.0	41.0
49	35.10553659220012	37.0	34.0	40.0	27.0	41.0
50	34.976434775963526	36.0	34.0	40.0	27.0	41.0
51	33.44023613623048	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	8.0
12	12.0
13	24.0
14	30.0
15	37.0
16	81.0
17	146.0
18	325.0
19	513.0
20	834.0
21	1272.0
22	1831.0
23	2656.0
24	3746.0
25	4837.0
26	6048.0
27	7675.0
28	9423.0
29	11532.0
30	14754.0
31	18679.0
32	24257.0
33	33701.0
34	60921.0
35	76468.0
36	64859.0
37	92784.0
38	146816.0
39	200760.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02717986601116	20.25918944392083	25.16162722571771	10.552003464350307
2	30.27702065873602	24.267773900196143	26.80732098733984	18.647884453727997
3	33.048118806836996	24.779657131212268	25.745090047634818	16.427134014315918
4	28.283872939857858	26.182209542247243	25.24543393534911	20.288483582545787
5	24.99490536719566	31.276460249127545	24.037496497439946	19.691137886236852
6	24.76679318338131	37.11478207708179	22.993988333290876	15.12443640624602
7	84.47563491861324	5.663575922765367	5.868889624780294	3.9918995338410985
8	86.48814224214789	4.526581246656647	5.117303920319943	3.8679725908755125
9	80.69923835239575	7.097715057187254	7.449244720686757	4.753801869730239
10	42.96074585424255	26.09292610235117	15.553786585831828	15.392541457574445
11	35.4427235906972	27.40339302544769	18.720100873729525	18.43378251012558
12	30.997401737269787	23.27457523498994	26.274677127646022	19.453345900094252
13	25.312046259265863	28.777415492778356	25.592633160964922	20.317905086990855
14	20.980207351555137	29.653564969304835	28.000229258476196	21.365998420663832
15	20.958555162136687	26.750133734111113	31.300914486588375	20.990396617163817
16	23.27966986779428	23.690552003464347	32.12968387803449	20.90009425070688
17	22.746516544820032	24.524033930254475	26.757393585857297	25.972055939068188
18	25.42896808212548	24.139516519346866	27.41472858343735	23.016786815090303
19	25.76954428509565	28.08594645540923	23.63387421351606	22.51063504597906
20	28.299156838270882	25.504368647629722	24.45602058231653	21.740453931782866
21	26.69511169982424	27.939730493924653	23.845428840716306	21.51972896553481
22	24.421122347606797	25.0463611585195	24.73749904475635	25.795017449117353
23	24.219374888554906	28.307562982398043	23.765825203148484	23.707236925898567
24	24.440099854802966	25.099217973864533	27.84114934916066	22.61953282217184
25	24.392337672262272	27.111343199938865	25.046743255979827	23.44957587181904
26	22.238326922587053	28.81829992103319	25.633899686680085	23.30947346969967
27	23.684438444099143	28.126066688743407	24.61573732073261	23.573757546424844
28	20.41775988995593	29.849326234811624	27.355885574547212	22.37702830068523
29	23.3113839570013	28.703670682935527	25.554932878212806	22.43001248185037
30	24.235168250248364	26.94156456173421	26.603026211885776	22.220240976131645
31	25.63784802710345	29.554346995440305	22.735053621010266	22.072751356445984
32	25.9945996892274	27.700792215401076	23.74047940494689	22.56412869042464
33	26.00249637007413	28.541024530656955	21.063504597906103	24.39297450136281
34	23.314440736683903	29.00374455511119	25.086099294393353	22.595715413811547
35	23.217133250120998	29.125761010775147	23.460147234888044	24.19695850421581
36	24.546959777874008	30.879970451129736	22.974246631174058	21.598823139822198
37	24.19925108897776	31.144891356955444	22.578393662276792	22.07746389179
38	24.257966732047787	29.56950352803322	22.912983671701863	23.259546068217134
39	25.87678630562702	26.76006826807958	22.43752706523677	24.925618361056625
40	25.12239855312428	25.506024403291132	26.29110731844002	23.08046972514456
41	21.591181190615686	28.3293425376366	24.417683470463867	25.661792801283845
42	23.623302850447054	27.394732149680312	24.463153068242608	24.518811931630026
43	23.459255674147286	26.498458873576684	24.49117354866648	25.551111903609545
44	23.28310874493721	26.89864228035764	25.35089283439896	24.467356140306187
45	23.849759278599993	26.773314313370868	23.889370048653742	25.487556359375397
46	24.244211223476068	27.817714038260693	23.802379193519627	24.135695544743612
47	22.805359553710165	28.114476399113535	25.679369284458826	23.400794762717478
48	23.56293145171562	25.648292024352344	26.57271314669995	24.216063377232086
49	23.74544667193112	26.89291081845276	25.540158443080212	23.821484066535906
50	21.999133912423265	26.578062511144505	26.548513640879335	24.874289935552895
51	21.888325648928852	27.007285324910207	24.6275823420027	26.476806684158237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1353.0
1	1273.5
2	1194.0
3	1451.0
4	1708.0
5	1683.0
6	1658.0
7	2347.0
8	3036.0
9	3400.5
10	3765.0
11	3762.0
12	3759.0
13	3442.0
14	3125.0
15	2740.0
16	2355.0
17	2220.0
18	2085.0
19	2255.5
20	2426.0
21	2737.5
22	3049.0
23	2950.0
24	2851.0
25	3756.5
26	5031.0
27	5400.0
28	6841.0
29	8282.0
30	9281.5
31	10281.0
32	11238.0
33	12195.0
34	13908.0
35	15621.0
36	17421.5
37	19222.0
38	20998.0
39	22774.0
40	25493.0
41	28212.0
42	33893.0
43	39574.0
44	43201.0
45	46828.0
46	51045.5
47	55263.0
48	59806.5
49	64350.0
50	63531.0
51	62712.0
52	58595.5
53	54479.0
54	51239.5
55	48000.0
56	46125.5
57	44251.0
58	42301.5
59	40352.0
60	40377.0
61	40402.0
62	38644.0
63	36886.0
64	31338.0
65	25790.0
66	22245.5
67	18701.0
68	15572.0
69	12443.0
70	11893.0
71	11343.0
72	10332.5
73	9322.0
74	7831.5
75	5089.0
76	3837.0
77	3040.0
78	2243.0
79	1744.5
80	1246.0
81	1059.0
82	872.0
83	724.5
84	577.0
85	376.0
86	175.0
87	121.0
88	67.0
89	61.0
90	55.0
91	31.5
92	8.0
93	8.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	785140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.54791592504828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28870528963456	18.727287234807353
2	9.17772940544069	4.505882596552028
3	3.1928984838089023	2.3513701061326517
4	1.5074368219814287	1.4801772947328833
5	0.9347670855512626	1.1473291912807406
6	0.6701146639785228	0.9869951058892052
7	0.5147089588110654	0.884452257273422
8	0.4077213949611548	0.8006968419479865
9	0.3216499990614204	0.7106253420826444
>10	4.9459808748094645	30.73618193115253
>50	1.8970866684463392	31.768959323030977
>100	0.13651104288187735	4.99090743627359
>500	0.0036472416037142807	0.5998656076503199
>1k	0.0010420690296326517	0.3092697311937044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	1410	0.17958580635300708	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1008	0.1283847466693838	No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	887	0.11297348243625341	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	789	0.10049163206561887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2736582010851568E-4	0.0	0.0	0.11972387090200474	0.0
2	1.2736582010851568E-4	0.0	0.0	0.54487097842423	0.0
3	1.2736582010851568E-4	0.0	0.0	0.7263672720788649	0.0
4	1.2736582010851568E-4	0.0	0.0	0.9483658965280077	0.0
5	1.2736582010851568E-4	0.0	0.0	1.6232773772830322	0.0
6	1.2736582010851568E-4	0.0	0.0	2.1496803117915277	0.0
7	1.2736582010851568E-4	0.0	0.0	2.600045851695239	0.0
8	1.2736582010851568E-4	0.0	0.0	3.4421886542527447	0.0
9	1.2736582010851568E-4	0.0	0.0	3.773212420714777	0.0
10	1.2736582010851568E-4	0.0	0.0	4.4499070229513205	0.0
11	1.2736582010851568E-4	0.0	0.0	5.292941386249586	0.0
12	1.2736582010851568E-4	0.0	0.0	5.934100924675854	0.0
13	1.2736582010851568E-4	0.0	0.0	6.194818758437986	0.0
14	1.2736582010851568E-4	0.0	0.0	6.297602975265558	0.0
15	1.2736582010851568E-4	0.0	0.0	6.5087755050054765	0.0
16	1.2736582010851568E-4	0.0	0.0	7.016583029778129	0.0
17	1.2736582010851568E-4	0.0	0.0	7.604758387039254	0.0
18	1.2736582010851568E-4	0.0	0.0	8.315077565784446	0.0
19	1.2736582010851568E-4	0.0	0.0	8.61336831647859	1.2736582010851568E-4
20	1.2736582010851568E-4	0.0	0.0	8.921084137860763	1.2736582010851568E-4
21	1.2736582010851568E-4	0.0	0.0	9.28445882263036	1.2736582010851568E-4
22	1.2736582010851568E-4	0.0	0.0	9.665664722215146	1.2736582010851568E-4
23	1.2736582010851568E-4	0.0	0.0	10.028912041164633	1.2736582010851568E-4
24	1.2736582010851568E-4	0.0	0.0	10.3484728838169	1.2736582010851568E-4
25	1.2736582010851568E-4	0.0	0.0	10.625748274193137	1.2736582010851568E-4
26	1.2736582010851568E-4	0.0	0.0	10.883664059912881	1.2736582010851568E-4
27	1.2736582010851568E-4	0.0	0.0	11.149349160659245	1.2736582010851568E-4
28	1.2736582010851568E-4	0.0	0.0	11.428152940876787	1.2736582010851568E-4
29	3.82097460325547E-4	0.0	0.0	11.701352625009552	1.2736582010851568E-4
30	3.82097460325547E-4	0.0	0.0	12.033395318032452	1.2736582010851568E-4
31	3.82097460325547E-4	0.0	0.0	12.331686068726597	1.2736582010851568E-4
32	3.82097460325547E-4	0.0	0.0	12.61532975010826	1.2736582010851568E-4
33	3.82097460325547E-4	0.0	0.0	12.90814377053774	1.2736582010851568E-4
34	3.82097460325547E-4	0.0	0.0	13.177649845887357	1.2736582010851568E-4
35	3.82097460325547E-4	0.0	0.0	13.477468986422803	1.2736582010851568E-4
36	3.82097460325547E-4	0.0	0.0	13.781873296482155	1.2736582010851568E-4
37	3.82097460325547E-4	0.0	0.0	14.08793336220292	1.2736582010851568E-4
38	3.82097460325547E-4	0.0	0.0	14.383804162315002	1.2736582010851568E-4
39	3.82097460325547E-4	0.0	0.0	14.685024326871641	1.2736582010851568E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.031919E-4	45.0	11
GGTACGC	25	3.8896724E-5	45.0	8
CACGCGT	20	7.031919E-4	45.0	40
CGACAAT	40	6.8102963E-9	45.0	20
GTTCGGA	20	7.031919E-4	45.0	31
CGGATTC	25	3.8896724E-5	45.0	20
TCGCTAA	25	3.8896724E-5	45.0	30
CGGTCTA	40	6.8102963E-9	45.0	31
ATAACGG	20	7.031919E-4	45.0	2
CGATCAC	20	7.031919E-4	45.0	29
TCGGTAG	20	7.031919E-4	45.0	2
ATCGATC	20	7.031919E-4	45.0	17
AATCGAT	20	7.031919E-4	45.0	16
TAATCCG	25	3.8896724E-5	45.0	16
AACGTAG	30	2.1646192E-6	44.999996	1
TTGCGTC	30	2.1646192E-6	44.999996	20
CGTACGT	30	2.1646192E-6	44.999996	14
CGTGTTA	30	2.1646192E-6	44.999996	41
TACGCAT	130	0.0	43.26923	16
CGCACGG	85	0.0	42.35294	2
>>END_MODULE