FastQCFastQC Report
Sat 18 Jun 2016
SRR3554057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567466
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC12730.22433062068916904No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG12530.22080618045838868No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC10950.19296310263522395No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC9290.16371024871974707No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8020.14133005325429188No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT7450.1312853985965679Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC7160.12617496026193642No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6800.11983096784653179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.2104829E-745.0000046
CGTATTT207.0302637E-445.00000429
CTATCGA207.0302637E-445.00000442
GTAGGTC207.0302637E-445.0000044
CCGTACA207.0302637E-445.00000420
ACTCGAT207.0302637E-445.00000433
TACTCGA351.2104829E-745.00000442
ATAGTCG207.0302637E-445.00000437
AGTAGGT207.0302637E-445.0000043
CCGCACG207.0302637E-445.00000437
TACGTAA207.0302637E-445.0000041
TCACGCA253.8883E-545.035
TAGCCGT253.8883E-545.044
ATTGACG253.8883E-545.01
GCGTAAG551.8189894E-1245.01
CGAATAT850.042.35294314
CGACAAT451.9254003E-840.020
TACGCGG403.4540972E-739.3750042
AAGTTAG403.4540972E-739.3750041
AAGGGTA1800.038.755