##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567466
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14739737711158	33.0	31.0	34.0	30.0	34.0
2	32.25147585934664	34.0	31.0	34.0	30.0	34.0
3	32.27502088230837	34.0	31.0	34.0	30.0	34.0
4	35.83973665382596	37.0	35.0	37.0	35.0	37.0
5	35.71552128233233	37.0	35.0	37.0	35.0	37.0
6	35.70954206948081	37.0	35.0	37.0	33.0	37.0
7	35.89372226706094	37.0	35.0	37.0	35.0	37.0
8	35.72120444220447	37.0	35.0	37.0	35.0	37.0
9	37.449878935478075	39.0	37.0	39.0	35.0	39.0
10	37.070039438486184	39.0	37.0	39.0	33.0	39.0
11	37.02652846161708	39.0	37.0	39.0	33.0	39.0
12	36.79600716166255	39.0	35.0	39.0	33.0	39.0
13	36.70360162547183	39.0	35.0	39.0	33.0	39.0
14	37.837715387353605	40.0	36.0	41.0	33.0	41.0
15	37.963647161239614	40.0	36.0	41.0	33.0	41.0
16	38.0517440692482	40.0	36.0	41.0	33.0	41.0
17	37.98666704260696	40.0	36.0	41.0	33.0	41.0
18	37.9675839609774	40.0	36.0	41.0	33.0	41.0
19	37.93629926726888	40.0	36.0	41.0	33.0	41.0
20	37.858310453842165	40.0	36.0	41.0	33.0	41.0
21	37.73886188776068	40.0	36.0	41.0	33.0	41.0
22	37.71482872982698	40.0	36.0	41.0	33.0	41.0
23	37.63977929955275	40.0	35.0	41.0	33.0	41.0
24	37.60943210694561	40.0	35.0	41.0	33.0	41.0
25	37.500733083568	39.0	35.0	41.0	33.0	41.0
26	37.446495472856526	39.0	35.0	41.0	33.0	41.0
27	37.37728604004469	39.0	35.0	41.0	33.0	41.0
28	37.256283195821425	39.0	35.0	41.0	32.0	41.0
29	37.22895116183172	39.0	35.0	41.0	32.0	41.0
30	37.117286321999906	39.0	35.0	41.0	32.0	41.0
31	37.03712997783127	39.0	35.0	41.0	32.0	41.0
32	36.92248698600445	39.0	35.0	41.0	31.0	41.0
33	36.79751914652155	39.0	35.0	41.0	31.0	41.0
34	36.71552656899268	39.0	35.0	41.0	31.0	41.0
35	36.624895588458166	39.0	35.0	41.0	31.0	41.0
36	36.51810504946552	39.0	35.0	41.0	31.0	41.0
37	36.434011905559096	39.0	35.0	41.0	30.0	41.0
38	36.326086496812145	38.0	35.0	40.0	30.0	41.0
39	36.257180165860156	38.0	35.0	40.0	30.0	41.0
40	36.128513426355056	38.0	35.0	40.0	30.0	41.0
41	36.03428399234492	38.0	35.0	40.0	30.0	41.0
42	35.934819002371945	38.0	35.0	40.0	30.0	41.0
43	35.836487119933174	38.0	35.0	40.0	29.0	41.0
44	35.68532564065512	38.0	35.0	40.0	29.0	41.0
45	35.59914250369185	38.0	34.0	40.0	29.0	41.0
46	35.47916703379586	37.0	34.0	40.0	28.0	41.0
47	35.36325700570607	37.0	34.0	40.0	28.0	41.0
48	35.27455036953756	37.0	34.0	40.0	28.0	41.0
49	35.19139825117276	37.0	34.0	40.0	28.0	41.0
50	35.07194087399069	37.0	34.0	40.0	27.0	41.0
51	33.563133297853966	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	3.0
12	9.0
13	9.0
14	15.0
15	31.0
16	52.0
17	115.0
18	204.0
19	383.0
20	647.0
21	979.0
22	1462.0
23	1972.0
24	2716.0
25	3505.0
26	4470.0
27	5536.0
28	6816.0
29	8504.0
30	10463.0
31	13266.0
32	17203.0
33	24258.0
34	42544.0
35	54880.0
36	47985.0
37	67876.0
38	107379.0
39	144122.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4495952180395	18.176948046226556	23.712786316713245	12.6606704190207
2	30.28886312131476	25.15533970317164	25.882960388816244	18.672836786697353
3	32.98840811608096	25.635897128638547	25.145823714548538	16.229871040731954
4	28.66303884285578	25.982878269358867	25.409451843810903	19.94463104397444
5	25.40011207719934	31.572992919399578	23.586082690416696	19.44081231298439
6	24.502965816454203	37.286110533494515	23.25319931061949	14.957724339431792
7	83.10348108961594	6.222927893477318	6.738024833205866	3.935566183700874
8	85.20633835331104	5.557513577905989	5.384287340563135	3.8518607282198407
9	79.90945713047125	6.640045394790173	7.204484497749646	6.246012976988929
10	43.90077995862307	25.545671458730567	14.827496272904456	15.726052309741906
11	34.255620601058034	25.881021946689316	20.370383423852704	19.49297402839994
12	29.544853788597024	23.185530058188508	26.18341891849027	21.086197234724192
13	25.969661618493443	26.48440611419891	27.640070065871786	19.905862201435855
14	22.63113560988676	28.798024903694667	26.656222575449455	21.914616910969116
15	21.378901995890505	24.96924925898644	32.182016191278414	21.469832553844636
16	23.980819996264096	23.649346392559202	29.01160598167996	23.358227629496746
17	23.257957304931047	24.311941155945906	26.288447237367524	26.141654301755523
18	25.57228098247296	24.31546559617669	27.44270141294809	22.669552008402267
19	26.846718569923134	25.716254365900337	24.353000884634497	23.084026179542033
20	27.560593938667694	25.297022200449014	24.69504780903173	22.447336051851565
21	26.282631910986737	27.136251334881738	25.04097161768282	21.540145136448704
22	24.9627290445595	24.17484043096855	24.582970609692918	26.279459914779036
23	23.90257742314077	27.418382775355703	24.713374898231788	23.965664903271737
24	24.046374584556606	25.341958813391464	27.05289832342378	23.558768278628147
25	24.04373125438352	26.958971991273483	25.497034183545797	23.500262570797194
26	22.445926275759255	27.882551553749472	24.96554859674412	24.70597357374715
27	23.431535986296975	27.301018915670717	25.38671920432237	23.88072589370993
28	21.996383924323222	27.355471517236275	27.87990822357639	22.768236334864113
29	23.83825638892903	26.635604600099388	25.849125762600757	23.677013248370827
30	23.919318514236977	27.502969340894435	25.54743367884596	23.030278466022633
31	27.230177667032034	26.586614880891542	22.542143494059555	23.64106395801687
32	27.454332065709664	27.29925669555533	22.96842453997244	22.277986698762568
33	25.54443790464979	26.780106649561386	22.518882188536406	25.15657325725242
34	23.877377675490692	27.626677192994826	23.80776998093278	24.68817515058171
35	24.90669044489009	27.117043135623987	24.44939432494634	23.526872094539584
36	26.262013935636674	29.21937173328446	22.760658788367937	21.75795554271093
37	25.227766949914184	28.801196899902372	23.391181145654542	22.579855004528905
38	25.15075793087163	29.74645176979766	22.124673548723624	22.978116750607086
39	25.232348722214194	27.513013995552154	23.557358502535834	23.697278779697815
40	25.143004162363912	25.793968272989044	25.8764401743893	23.186587390257742
41	22.52663595704412	26.20068867562109	25.061589593032885	26.211085774301896
42	24.367274867569158	26.63348993596092	23.988045098737192	25.011190097732726
43	25.27094134274124	24.766065279681953	25.408218289730133	24.55477508784667
44	24.000733083568	25.544085460626714	25.878731060539312	24.576450395265972
45	24.39212217119616	25.967370732343436	24.647644087927734	24.99286300853267
46	25.343897255518392	26.568287791691485	24.561647746296693	23.52616720649343
47	23.489513028093313	26.47348034948349	26.204741781886494	23.832264840536702
48	23.63207663542838	26.534629387487534	25.624442697888504	24.20885127919558
49	23.94751403608322	25.555716113388293	27.231763665135883	23.265006185392608
50	22.925426369156916	25.983935601428104	26.95668110512348	24.1339569242915
51	23.163502306746132	26.139363415605516	24.24691523368801	26.450219043960345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	600.0
1	728.0
2	856.0
3	1039.0
4	1222.0
5	994.0
6	766.0
7	749.0
8	732.0
9	817.0
10	902.0
11	906.5
12	911.0
13	939.5
14	968.0
15	954.5
16	941.0
17	997.5
18	1054.0
19	1035.5
20	1017.0
21	1137.0
22	1257.0
23	1464.5
24	1672.0
25	2552.0
26	3556.5
27	3681.0
28	4705.0
29	5729.0
30	6595.0
31	7461.0
32	8093.5
33	8726.0
34	10072.0
35	11418.0
36	12481.5
37	13545.0
38	14292.0
39	15039.0
40	18458.5
41	21878.0
42	24854.0
43	27830.0
44	30901.5
45	33973.0
46	39243.0
47	44513.0
48	47863.5
49	51214.0
50	49826.0
51	48438.0
52	45029.0
53	41620.0
54	38550.5
55	35481.0
56	33459.0
57	31437.0
58	30698.5
59	29960.0
60	27795.5
61	25631.0
62	24412.5
63	23194.0
64	20564.5
65	17935.0
66	16064.5
67	14194.0
68	12459.0
69	10724.0
70	9547.0
71	8370.0
72	7479.0
73	6588.0
74	5799.0
75	4258.5
76	3507.0
77	2743.5
78	1980.0
79	1520.5
80	1061.0
81	783.0
82	505.0
83	389.0
84	273.0
85	184.0
86	95.0
87	75.0
88	55.0
89	40.0
90	25.0
91	24.0
92	23.0
93	17.0
94	11.0
95	11.5
96	12.0
97	6.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.471309717122466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35228075707808	18.9384997133798
2	8.69837528310343	4.431180217433805
3	3.0075576631307253	2.298192981891233
4	1.6095808062741752	1.6399252492538086
5	1.074521815664754	1.3684738982300861
6	0.7864404090349735	1.201900033955417
7	0.5699611190976254	1.0162359333877335
8	0.5597572678240735	1.1406200588125797
9	0.4748732550741139	1.0886079380733766
>10	8.035104643717855	50.914366749929776
>50	0.7669945015861377	11.749747035681228
>100	0.05761135170285016	2.7025585059349253
>500	0.004858788697830736	0.8695003749959416
>1k	0.00208233801335603	0.6401913090403221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	1273	0.22433062068916904	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG	1253	0.22080618045838868	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC	1095	0.19296310263522395	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	929	0.16371024871974707	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	802	0.14133005325429188	No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT	745	0.1312853985965679	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	716	0.12617496026193642	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	680	0.11983096784653179	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13903916710428466	0.0
2	0.0	0.0	0.0	0.6465585603366545	0.0
3	0.0	0.0	0.0	0.8405789950411126	0.0
4	0.0	0.0	0.0	1.099801574015007	0.0
5	0.0	0.0	0.0	1.9668138707869722	0.0
6	0.0	0.0	0.0	2.5457031786926443	0.0
7	0.0	0.0	0.0	3.0576281222134893	0.0
8	0.0	0.0	0.0	3.9040224436353896	0.0
9	0.0	0.0	0.0	4.235496047340281	0.0
10	0.0	0.0	0.0	5.117839659116141	0.0
11	0.0	0.0	0.0	5.987319064049652	0.0
12	0.0	0.0	0.0	6.864023571456263	0.0
13	0.0	0.0	0.0	7.147741010034081	0.0
14	0.0	0.0	0.0	7.261756651499826	0.0
15	0.0	0.0	0.0	7.49419348471979	0.0
16	0.0	0.0	0.0	7.922412972759601	0.0
17	0.0	0.0	0.0	8.479450751234435	0.0
18	0.0	0.0	0.0	9.08671180299789	0.0
19	0.0	0.0	0.0	9.43439783176437	0.0
20	0.0	0.0	0.0	9.748777900349976	0.0
21	0.0	0.0	0.0	10.100340813370316	0.0
22	0.0	0.0	0.0	10.479218138179204	0.0
23	0.0	0.0	0.0	10.863205901322722	0.0
24	0.0	0.0	0.0	11.188511734623749	0.0
25	0.0	0.0	0.0	11.46447540469385	0.0
26	0.0	0.0	0.0	11.721583319529275	0.0
27	1.7622201153901732E-4	0.0	0.0	11.99331766132244	0.0
28	1.7622201153901732E-4	0.0	0.0	12.271219773519471	0.0
29	1.7622201153901732E-4	0.0	0.0	12.550355439797274	0.0
30	1.7622201153901732E-4	0.0	0.0	12.875485051086761	0.0
31	1.7622201153901732E-4	0.0	0.0	13.16977581035692	0.0
32	1.7622201153901732E-4	0.0	0.0	13.463537903592462	0.0
33	1.7622201153901732E-4	0.0	0.0	13.75994332700109	0.0
34	3.5244402307803464E-4	0.0	0.0	14.035730775059651	0.0
35	5.28666034617052E-4	0.0	0.0	14.346586403414477	0.0
36	5.28666034617052E-4	0.0	0.0	14.626250735726899	0.0
37	5.28666034617052E-4	0.0	0.0	14.916840832754739	0.0
38	5.28666034617052E-4	0.0	0.0	15.21588958633645	0.0
39	5.28666034617052E-4	0.0	0.0	15.521634776356645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2104829E-7	45.000004	6
CGTATTT	20	7.0302637E-4	45.000004	29
CTATCGA	20	7.0302637E-4	45.000004	42
GTAGGTC	20	7.0302637E-4	45.000004	4
CCGTACA	20	7.0302637E-4	45.000004	20
ACTCGAT	20	7.0302637E-4	45.000004	33
TACTCGA	35	1.2104829E-7	45.000004	42
ATAGTCG	20	7.0302637E-4	45.000004	37
AGTAGGT	20	7.0302637E-4	45.000004	3
CCGCACG	20	7.0302637E-4	45.000004	37
TACGTAA	20	7.0302637E-4	45.000004	1
TCACGCA	25	3.8883E-5	45.0	35
TAGCCGT	25	3.8883E-5	45.0	44
ATTGACG	25	3.8883E-5	45.0	1
GCGTAAG	55	1.8189894E-12	45.0	1
CGAATAT	85	0.0	42.352943	14
CGACAAT	45	1.9254003E-8	40.0	20
TACGCGG	40	3.4540972E-7	39.375004	2
AAGTTAG	40	3.4540972E-7	39.375004	1
AAGGGTA	180	0.0	38.75	5
>>END_MODULE