##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302444
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.929950668553516	33.0	31.0	34.0	30.0	34.0
2	32.046983904458344	33.0	31.0	34.0	30.0	34.0
3	32.06714962108688	33.0	31.0	34.0	30.0	34.0
4	35.66244660168494	37.0	35.0	37.0	33.0	37.0
5	35.54437185065665	37.0	35.0	37.0	33.0	37.0
6	35.52251987144728	37.0	35.0	37.0	33.0	37.0
7	35.80241631508643	37.0	35.0	37.0	35.0	37.0
8	35.63981100633506	37.0	35.0	37.0	35.0	37.0
9	37.30356032852363	39.0	37.0	39.0	34.0	39.0
10	36.88994987501818	39.0	37.0	39.0	33.0	39.0
11	36.87433045456349	39.0	37.0	39.0	33.0	39.0
12	36.784872571451245	39.0	35.0	39.0	33.0	39.0
13	36.76912750790229	39.0	35.0	39.0	33.0	39.0
14	37.84098543862665	40.0	37.0	41.0	33.0	41.0
15	37.96000912565632	40.0	37.0	41.0	33.0	41.0
16	38.01813558873709	40.0	37.0	41.0	33.0	41.0
17	37.981930539207255	40.0	37.0	41.0	33.0	41.0
18	37.939324304664666	40.0	37.0	41.0	33.0	41.0
19	37.91384851410509	40.0	37.0	41.0	33.0	41.0
20	37.864209572681226	40.0	36.0	41.0	33.0	41.0
21	37.751646585814235	40.0	36.0	41.0	33.0	41.0
22	37.7487601010435	40.0	36.0	41.0	33.0	41.0
23	37.69148999484202	39.0	36.0	41.0	33.0	41.0
24	37.683587705492585	39.0	36.0	41.0	33.0	41.0
25	37.577902024837655	39.0	36.0	41.0	33.0	41.0
26	37.54980756768195	39.0	36.0	41.0	33.0	41.0
27	37.53225390485511	39.0	36.0	41.0	33.0	41.0
28	37.425754850484715	39.0	36.0	41.0	32.0	41.0
29	37.368507889063764	39.0	36.0	41.0	32.0	41.0
30	37.24997354882226	39.0	35.0	41.0	32.0	41.0
31	37.184811072463	39.0	35.0	41.0	32.0	41.0
32	37.08789065083123	39.0	35.0	41.0	31.0	41.0
33	36.99856833000489	39.0	35.0	41.0	31.0	41.0
34	36.90935512028673	39.0	35.0	41.0	31.0	41.0
35	36.860324555950854	39.0	35.0	41.0	31.0	41.0
36	36.75856687519012	39.0	35.0	41.0	31.0	41.0
37	36.703211172977475	39.0	35.0	41.0	31.0	41.0
38	36.58847918953592	39.0	35.0	40.0	30.0	41.0
39	36.52841517768579	39.0	35.0	40.0	30.0	41.0
40	36.39016148444009	39.0	35.0	40.0	30.0	41.0
41	36.28442951422412	38.0	35.0	40.0	30.0	41.0
42	36.18565420375342	38.0	35.0	40.0	30.0	41.0
43	36.09575656981127	38.0	35.0	40.0	30.0	41.0
44	35.97749335414159	38.0	35.0	40.0	29.0	41.0
45	35.92256087077277	38.0	35.0	40.0	29.0	41.0
46	35.77100884791896	38.0	34.0	40.0	29.0	41.0
47	35.67340730846041	38.0	34.0	40.0	28.0	41.0
48	35.57118342569203	38.0	34.0	40.0	28.0	41.0
49	35.492110936239435	38.0	34.0	40.0	28.0	41.0
50	35.37925037362289	37.0	34.0	40.0	28.0	41.0
51	33.77339276031265	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	3.0
14	8.0
15	13.0
16	31.0
17	48.0
18	80.0
19	171.0
20	287.0
21	431.0
22	675.0
23	880.0
24	1261.0
25	1715.0
26	2158.0
27	2768.0
28	3547.0
29	4535.0
30	5695.0
31	7358.0
32	9366.0
33	12880.0
34	20666.0
35	25476.0
36	27007.0
37	38849.0
38	61657.0
39	74847.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52394492864794	19.45649442541429	22.618402084352805	13.401158561584955
2	30.203938580365293	25.3954451071934	25.472153522635594	18.928462789805717
3	32.55842403883033	24.789051857533956	25.575313115816485	17.07721098781923
4	28.950483395273174	26.45051645924535	24.63828014442343	19.960720001058046
5	24.622409437780217	30.853976273293572	24.100990596606316	20.4226236923199
6	24.692835698509477	36.8236764491939	23.716125960508393	14.767361891788234
7	81.49244157596117	5.636745976114586	7.957175543241063	4.913636904683181
8	81.38432238695428	5.001256430942588	7.514779595561492	6.099641586541641
9	75.02380605996481	7.279694753408895	10.453505442329819	7.242993744296465
10	38.84454642842973	24.95139596090516	17.88099615135364	18.323061459311475
11	30.128552723810024	25.681448466492974	23.244964357037997	20.945034452659005
12	26.342066630516726	22.63030511433522	29.553239607993547	21.474388647154516
13	24.33971247569798	24.26631045747312	30.445966856674296	20.948010210154607
14	20.975122667336763	28.285236275145152	28.097102273478725	22.64253878403936
15	20.267884302548573	26.300736665300022	31.860112946528947	21.57126608562246
16	22.381664043591538	25.005951514991203	29.694092129452066	22.918292311965192
17	22.066895028501143	24.674319874092394	27.80812315668355	25.450661940722913
18	23.173546177143535	24.970903704487444	29.167383052730422	22.6881670656386
19	24.231593286691087	27.34555818597823	25.890743410350346	22.532105116980333
20	24.680932668527067	26.18534340241499	26.11094946502493	23.02277446403301
21	24.35624446178466	27.87193662297814	26.67865786724154	21.09316104799566
22	22.337027681157505	24.90014680403645	25.879171020089668	26.883654494716374
23	21.517371810979885	27.26124505693616	26.372485484916215	24.84889764716774
24	22.449775826268663	25.40767877689754	27.837550091917844	24.304995304915952
25	22.114507148430786	26.76363227572708	26.11524778140747	25.006612794434673
26	20.643491026437953	27.221237650606394	27.177262567615823	24.95800875533983
27	23.283979844202563	25.464879448757454	26.40125114070704	24.84988956633294
28	20.848157014191056	27.209665260345716	27.394492864794806	24.54768486066842
29	22.041435769927656	25.84379256986417	26.981523852349525	25.133247807858645
30	22.708005448942615	25.40569493856714	26.290156194204545	25.5961434182857
31	23.204626310986495	27.471531919958736	23.118329343614025	26.20551242544074
32	22.922259988625992	27.859041673830525	24.297390591316077	24.9213077462274
33	21.966049913372395	26.81488143259579	23.404332702913596	27.814735951118223
34	20.62927351840341	26.774543386544288	25.942323206940788	26.65385988811152
35	21.688643186837893	25.492322545661345	25.283358241525704	27.535676025975057
36	22.594926664109718	27.83622753303091	23.763076800994565	25.80576900186481
37	21.884051262382457	28.35996085225695	24.923622224279534	24.83236566108106
38	22.26164182460224	29.46099112562987	23.393090952374653	24.884276097393236
39	22.38728491886101	26.404888177646107	24.722923913187234	26.484902990305642
40	23.872518548888387	23.135853248865907	27.5075716496277	25.484056552618007
41	21.376519289521365	25.455952176270646	26.138392561928818	27.029135972279168
42	22.337358320879236	26.624432952877225	26.46010501117562	24.578103715067915
43	21.584161034770073	25.182513126396955	27.371017444551722	25.862308394281253
44	21.88537382126939	25.16994881697108	26.503418814722725	26.441258547036806
45	22.312560341749215	23.835486900054224	26.230310404570762	27.6216423536258
46	23.47608152252979	24.465024930235018	25.86825990927246	26.19063363796273
47	21.385446562008173	25.174577773075345	27.880533255743213	25.559442409173265
48	21.27699673327955	24.89750168626258	27.61767467696499	26.207826903492876
49	22.11219267037865	24.334091600428508	28.335493512848657	25.218222216344184
50	20.94569573210247	22.986404094642314	28.432701591038338	27.635198582216873
51	20.83393950615651	23.25521418841174	26.41844440623719	29.492401899194558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	269.0
1	331.0
2	393.0
3	475.0
4	557.0
5	437.0
6	317.0
7	338.0
8	359.0
9	356.0
10	353.0
11	370.5
12	388.0
13	417.5
14	447.0
15	456.0
16	465.0
17	444.5
18	424.0
19	462.0
20	500.0
21	593.5
22	687.0
23	729.0
24	771.0
25	1199.0
26	1861.0
27	2095.0
28	2528.5
29	2962.0
30	3430.5
31	3899.0
32	4654.5
33	5410.0
34	5858.5
35	6307.0
36	6750.0
37	7193.0
38	7887.5
39	8582.0
40	9826.0
41	11070.0
42	13064.5
43	15059.0
44	16931.5
45	18804.0
46	22565.0
47	26326.0
48	29470.0
49	32614.0
50	32342.5
51	32071.0
52	28014.0
53	23957.0
54	21443.0
55	18929.0
56	17147.5
57	15366.0
58	14735.0
59	14104.0
60	13378.5
61	12653.0
62	11115.5
63	9578.0
64	8587.0
65	7596.0
66	6819.0
67	6042.0
68	5109.0
69	4176.0
70	3695.5
71	3215.0
72	2903.5
73	2592.0
74	2142.0
75	1367.0
76	1042.0
77	899.0
78	756.0
79	580.5
80	405.0
81	280.5
82	156.0
83	147.0
84	138.0
85	87.5
86	37.0
87	38.0
88	39.0
89	24.0
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	4.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.821981298735054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77081661274204	30.015977501730408
2	9.520905738015712	7.9636628344462475
3	3.9506938498510253	4.956775329164915
4	2.5533646963743153	4.271470823224678
5	2.0385115916540615	4.262729683170539
6	1.643800882803308	4.124820586766647
7	1.417174855525463	4.148834221337642
8	1.2834728969056983	4.294190359345873
9	0.9949803518450644	3.7450844700725794
>10	4.74408880841002	27.790883111981397
>50	0.05294914387991033	1.4873091750870724
>100	0.026079429075179718	2.0019181496671994
>500	0.003161142918203602	0.936343754004817
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	915	0.3025353453862533	No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	723	0.23905251881340014	No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	671	0.22185925328325243	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	524	0.17325521418841172	No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	460	0.15209427199746067	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	315	0.10415151234608722	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2304558860483263	0.0
2	0.0	0.0	0.0	1.0554019917736837	0.0
3	0.0	0.0	0.0	1.4842417108621762	0.0
4	0.0	0.0	0.0	1.9997090370448745	0.0
5	0.0	0.0	0.0	3.578844348044597	0.0
6	0.0	0.0	0.0	4.837920408406184	0.0
7	0.0	0.0	0.0	5.864556744389044	0.0
8	0.0	0.0	0.0	7.645712925368002	0.0
9	0.0	0.0	0.0	8.370475195408076	0.0
10	0.0	0.0	0.0	9.825620610757694	0.0
11	0.0	0.0	0.0	11.533705413233525	0.0
12	0.0	0.0	0.0	12.884699316237056	0.0
13	0.0	0.0	0.0	13.376691222176667	0.0
14	0.0	0.0	0.0	13.566809062173494	0.0
15	0.0	0.0	0.0	13.955972014653952	0.0
16	3.306397217336102E-4	0.0	0.0	14.737604316832208	0.0
17	3.306397217336102E-4	0.0	0.0	15.65281506659084	0.0
18	3.306397217336102E-4	0.0	0.0	16.75450661940723	0.0
19	3.306397217336102E-4	0.0	0.0	17.351972596579863	0.0
20	3.306397217336102E-4	0.0	0.0	17.96001904484797	0.0
21	3.306397217336102E-4	0.0	0.0	18.66791868907963	0.0
22	3.306397217336102E-4	0.0	0.0	19.382761767467695	0.0
23	3.306397217336102E-4	0.0	0.0	20.11116107444684	0.0
24	3.306397217336102E-4	0.0	0.0	20.727142876036556	0.0
25	9.919191652008306E-4	0.0	0.0	21.243932761106187	0.0
26	9.919191652008306E-4	0.0	0.0	21.723690997341656	0.0
27	9.919191652008306E-4	0.0	0.0	22.187578526933912	0.0
28	9.919191652008306E-4	0.0	0.0	22.685852587586464	0.0
29	9.919191652008306E-4	0.0	0.0	23.22148893679491	0.0
30	9.919191652008306E-4	0.0	0.0	23.783245824020316	0.0
31	9.919191652008306E-4	0.0	0.0	24.335744799037176	0.0
32	9.919191652008306E-4	0.0	0.0	24.8809697001759	0.0
33	9.919191652008306E-4	0.0	0.0	25.389162952480458	0.0
34	9.919191652008306E-4	0.0	0.0	25.886114454246076	0.0
35	9.919191652008306E-4	0.0	0.0	26.407533295419977	0.0
36	9.919191652008306E-4	0.0	0.0	26.91407334911587	0.0
37	9.919191652008306E-4	0.0	0.0	27.390194548412268	0.0
38	9.919191652008306E-4	0.0	0.0	27.887807329621353	0.0
39	9.919191652008306E-4	0.0	0.0	28.357977013926543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	25	3.883966E-5	45.000004	1
CATGCGG	25	3.883966E-5	45.000004	2
CGTTAGG	25	3.883966E-5	45.000004	2
GCGATTA	25	3.883966E-5	45.000004	9
ATAACGG	25	3.883966E-5	45.000004	2
TATCAGT	20	7.0250337E-4	45.0	22
ACGCATT	20	7.0250337E-4	45.0	17
TGAACGG	20	7.0250337E-4	45.0	2
AATGCGG	40	6.7866495E-9	45.0	2
GCCGATA	20	7.0250337E-4	45.0	9
TACGCAT	20	7.0250337E-4	45.0	16
CGCTAGG	20	7.0250337E-4	45.0	2
TATCGTA	20	7.0250337E-4	45.0	28
CGGGCGA	60	3.6379788E-12	41.250004	6
CCCTCGT	55	6.002665E-11	40.909092	14
CGCATGG	55	6.002665E-11	40.909092	2
TCGTGTA	55	6.002665E-11	40.909092	17
CGTGTAC	55	6.002665E-11	40.909092	18
ACAACGA	50	1.0750227E-9	40.500004	13
ATGACGG	50	1.0750227E-9	40.500004	2
>>END_MODULE